The Experts below are selected from a list of 2664 Experts worldwide ranked by ideXlab platform
Lorella Severino - One of the best experts on this subject based on the ideXlab platform.
-
investigation of the persistence of penicillin g and Dihydrostreptomycin residues in milk of lactating buffaloes bubalus bubalis using ultra high performance liquid chromatography and tandem mass spectrometry
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Claudia Chirollo, Antonio Vassallo, Fabrizio Dal Piaz, Barbara Lamagna, G Tortora, Gianluca Neglia, Nunziatina De Tommasi, Lorella SeverinoAbstract:The purpose of this research was to evaluate the persistence of penicillin G and Dihydrostreptomycin in milk of lactating buffaloes following intramuscular injection of procaine penicillin G (200000 IU/mL) and Dihydrostreptomycin sulfate (250 mg/mL) every 24 h for 3 days. Milk samples were collected twice daily up to the 13th milking post-treatment and analyzed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry. The analytical method has been validated according to Commission Decision 2002/657/EC. The highest concentrations of penicillin G (275 μg kg-1) and Dihydrostreptomycin (220.5 μg kg-1) were detected in the milk of the first milkings post-treatment, and levels were below the maximum residue limit of 4 and 200 μg kg-1 in all treated buffaloes at milkings 12 and 2, respectively. The results of this study demonstrate that a nine-milking withdrawal time set for bovine milk was not adequate for depletion of penicillin G in lactating buffaloes.
A. J. De Neeling - One of the best experts on this subject based on the ideXlab platform.
-
Bioavailability in the rabbit of penicillin and Dihydrostreptomycin from three commercial penicillin/aminoglycoside fixed combination products for intramuscular injection.
Journal of Veterinary Pharmacology and Therapeutics, 1996Co-Authors: K. Groen, D. P. K. H. Pereboom-de Fauw, A. Van Veen-rutgers, A. G. Vulto, A. J. De NeelingAbstract:: The bioavailability of penicillin and Dihydrostreptomycin from three penicillin/ aminoglycoside fixed combination products for intramuscular injection was investigated in a four-way, randomized, crossover experiment in rabbits. Attention is focused on bioequivalence based on plasma concentration vs. time profiles to study whether the rabbit is a good model to detect differences in in vivo delivery of penicillin and/or Dihydrostreptomycin after intramuscular administration of different products. In all products, penicillin was present as a suspension. Although the extent of absorption of penicillin did not differ between the three products, large differences in the rates of absorption were observed. With respect to Dihydrostreptomycin, no significant differences were observed between the products. The results from this study demonstrate that the rabbit is a good model to detect differences in bioavailability of suspended penicillin from penicillin /Dihydrostreptomycin fixed combination products for intramuscular injection. A study with the same products is presently being carried out in calves to investigate whether bioequivalence studies in rabbits could replace studies in the target animals.
-
bioavailability in the rabbit of penicillin and Dihydrostreptomycin from three commercial penicillin aminoglycoside fixed combination products for intramuscular injection
Journal of Veterinary Pharmacology and Therapeutics, 1996Co-Authors: K. Groen, D. P. K. H. Pereboom-de Fauw, A. G. Vulto, A Van Veenrutgers, A. J. De NeelingAbstract:The bioavailability of penicillin and Dihydrostreptomycin from three penicillin/ aminoglycoside fixed combination products for intramuscular injection was investigated in a four-way, randomized, crossover experiment in rabbits. Attention is focused on bioequivalence based on plasma concentration vs. time profiles to study whether the rabbit is a good model to detect differences in in vivo delivery of penicillin and/or Dihydrostreptomycin after intramuscular administration of different products. In all products, penicillin was present as a suspension. Although the extent of absorption of penicillin did not differ between the three products, large differences in the rates of absorption were observed. With respect to Dihydrostreptomycin, no significant differences were observed between the products. The results from this study demonstrate that the rabbit is a good model to detect differences in bioavailability of suspended penicillin from penicillin /Dihydrostreptomycin fixed combination products for intramuscular injection. A study with the same products is presently being carried out in calves to investigate whether bioequivalence studies in rabbits could replace studies in the target animals.
-
Bioequivalence study in calves of three commercial penicillin/ Dihydrostreptomycin fixed combination products for intramuscular injection
Journal of Veterinary Pharmacology and Therapeutics, 1996Co-Authors: K. Groen, D. P. K. H. Pereboom-de Fauw, A. J. De Neeling, D.j. Mevius, A. G. VultoAbstract:A bioequivalence study with three penicillin/Dihydrostreptomycin fixed combination products for intramuscular administration was performed in dairy calves. In addition to plasma concentrations of penicillin and Dihydrostreptomycin, creatine phosphokinase concentrations were determined during a period of 72 h after administration of the drug products. Considerable differences were observed in the pharmacokinetics of penicillin from the three products. Although the extent of absorption was similar for all products, one product showed a significantly slower release from the site of injection. Except for the AUC, the 90% confidence intervals for these parameters exceeded the acceptable range of 0.80-1.20. Therefore, these products are not bioequivalent with respect to the rate of absorption of penicillin. Concerning the pharmacokinetics of Dihydrostreptomycin in calves, it could not be concluded that the products were bioequivalent, since the 90% confidence intervals of the ratios for Cmax, tmax and MRT exceeded the range of 0.80-1.20. From this study in calves, it was also found that the product with the slowest release of penicillin from the injection site caused the most severe tissue damage, based on plasma creatine phosphokinase concentrations. Comparing the results from this study in calves with those from a previous study in rabbits, it can be concluded that the rabbit is a good animal model that could substitute for large animals, at least calves, in bioequivalence studies for penicillin/Dihydrostreptomycin fixed combination products.
K. Groen - One of the best experts on this subject based on the ideXlab platform.
-
Bioavailability in the rabbit of penicillin and Dihydrostreptomycin from three commercial penicillin/aminoglycoside fixed combination products for intramuscular injection.
Journal of Veterinary Pharmacology and Therapeutics, 1996Co-Authors: K. Groen, D. P. K. H. Pereboom-de Fauw, A. Van Veen-rutgers, A. G. Vulto, A. J. De NeelingAbstract:: The bioavailability of penicillin and Dihydrostreptomycin from three penicillin/ aminoglycoside fixed combination products for intramuscular injection was investigated in a four-way, randomized, crossover experiment in rabbits. Attention is focused on bioequivalence based on plasma concentration vs. time profiles to study whether the rabbit is a good model to detect differences in in vivo delivery of penicillin and/or Dihydrostreptomycin after intramuscular administration of different products. In all products, penicillin was present as a suspension. Although the extent of absorption of penicillin did not differ between the three products, large differences in the rates of absorption were observed. With respect to Dihydrostreptomycin, no significant differences were observed between the products. The results from this study demonstrate that the rabbit is a good model to detect differences in bioavailability of suspended penicillin from penicillin /Dihydrostreptomycin fixed combination products for intramuscular injection. A study with the same products is presently being carried out in calves to investigate whether bioequivalence studies in rabbits could replace studies in the target animals.
-
bioavailability in the rabbit of penicillin and Dihydrostreptomycin from three commercial penicillin aminoglycoside fixed combination products for intramuscular injection
Journal of Veterinary Pharmacology and Therapeutics, 1996Co-Authors: K. Groen, D. P. K. H. Pereboom-de Fauw, A. G. Vulto, A Van Veenrutgers, A. J. De NeelingAbstract:The bioavailability of penicillin and Dihydrostreptomycin from three penicillin/ aminoglycoside fixed combination products for intramuscular injection was investigated in a four-way, randomized, crossover experiment in rabbits. Attention is focused on bioequivalence based on plasma concentration vs. time profiles to study whether the rabbit is a good model to detect differences in in vivo delivery of penicillin and/or Dihydrostreptomycin after intramuscular administration of different products. In all products, penicillin was present as a suspension. Although the extent of absorption of penicillin did not differ between the three products, large differences in the rates of absorption were observed. With respect to Dihydrostreptomycin, no significant differences were observed between the products. The results from this study demonstrate that the rabbit is a good model to detect differences in bioavailability of suspended penicillin from penicillin /Dihydrostreptomycin fixed combination products for intramuscular injection. A study with the same products is presently being carried out in calves to investigate whether bioequivalence studies in rabbits could replace studies in the target animals.
-
Bioequivalence study in calves of three commercial penicillin/ Dihydrostreptomycin fixed combination products for intramuscular injection
Journal of Veterinary Pharmacology and Therapeutics, 1996Co-Authors: K. Groen, D. P. K. H. Pereboom-de Fauw, A. J. De Neeling, D.j. Mevius, A. G. VultoAbstract:A bioequivalence study with three penicillin/Dihydrostreptomycin fixed combination products for intramuscular administration was performed in dairy calves. In addition to plasma concentrations of penicillin and Dihydrostreptomycin, creatine phosphokinase concentrations were determined during a period of 72 h after administration of the drug products. Considerable differences were observed in the pharmacokinetics of penicillin from the three products. Although the extent of absorption was similar for all products, one product showed a significantly slower release from the site of injection. Except for the AUC, the 90% confidence intervals for these parameters exceeded the acceptable range of 0.80-1.20. Therefore, these products are not bioequivalent with respect to the rate of absorption of penicillin. Concerning the pharmacokinetics of Dihydrostreptomycin in calves, it could not be concluded that the products were bioequivalent, since the 90% confidence intervals of the ratios for Cmax, tmax and MRT exceeded the range of 0.80-1.20. From this study in calves, it was also found that the product with the slowest release of penicillin from the injection site caused the most severe tissue damage, based on plasma creatine phosphokinase concentrations. Comparing the results from this study in calves with those from a previous study in rabbits, it can be concluded that the rabbit is a good animal model that could substitute for large animals, at least calves, in bioequivalence studies for penicillin/Dihydrostreptomycin fixed combination products.
Claudia Chirollo - One of the best experts on this subject based on the ideXlab platform.
-
investigation of the persistence of penicillin g and Dihydrostreptomycin residues in milk of lactating buffaloes bubalus bubalis using ultra high performance liquid chromatography and tandem mass spectrometry
Journal of Agricultural and Food Chemistry, 2018Co-Authors: Claudia Chirollo, Antonio Vassallo, Fabrizio Dal Piaz, Barbara Lamagna, G Tortora, Gianluca Neglia, Nunziatina De Tommasi, Lorella SeverinoAbstract:The purpose of this research was to evaluate the persistence of penicillin G and Dihydrostreptomycin in milk of lactating buffaloes following intramuscular injection of procaine penicillin G (200000 IU/mL) and Dihydrostreptomycin sulfate (250 mg/mL) every 24 h for 3 days. Milk samples were collected twice daily up to the 13th milking post-treatment and analyzed by ultra-high-performance liquid chromatography coupled to tandem mass spectrometry. The analytical method has been validated according to Commission Decision 2002/657/EC. The highest concentrations of penicillin G (275 μg kg-1) and Dihydrostreptomycin (220.5 μg kg-1) were detected in the milk of the first milkings post-treatment, and levels were below the maximum residue limit of 4 and 200 μg kg-1 in all treated buffaloes at milkings 12 and 2, respectively. The results of this study demonstrate that a nine-milking withdrawal time set for bovine milk was not adequate for depletion of penicillin G in lactating buffaloes.
Basilio Morelli - One of the best experts on this subject based on the ideXlab platform.
-
determination of a ternary mixture of penicillin g sodium salt penicillin g procain salt and Dihydrostreptomycin sulphate by third derivative spectrophotometry
Talanta, 1994Co-Authors: Basilio MorelliAbstract:Abstract Ternary mixtures of antibiotics, i.e. penicillin-G sodium salt, penicillin-G procain salt and Dihydrostreptomycin sulphate salt, are assayed by ‘zero-crossing’ third-derivative spectrophotometry. Calibration plots follows Beer's law up to 40 μg/ml of penicillin-G sodium at 222.5 nm ( r = 0.9997), 46 μg/ml of penicillin-G procain at 217 nm ( r = 0.9999) and 36 μg/ml of Dihydrostreptomycin sulphate at 211.5 nm ( r = 0.9999), in the presence of one another. Detection limits at p = 0.05 level of significance were calculated to be, respectively, 0.66, 0.41 and 0.25 μg/ml. The procedure is rapid, simple, nondestructive and does not require solution of equations. The method was successfully applied for determining laboratory mixtures and commercial injections.