The Experts below are selected from a list of 243 Experts worldwide ranked by ideXlab platform
Tsehay Kassa - One of the best experts on this subject based on the ideXlab platform.
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cost effectiveness analysis of metronidazole versus metronidazole with Diloxanide furoate in the treatment of amoebiasis in ethiopia
ClinicoEconomics and Outcomes Research, 2021Co-Authors: Nanati Legese, Temesgen Aferu, Tsehay KassaAbstract:Introduction Amoebiasis is one of the world's most prevalent and fatal infectious diseases. Several surveys revealed that amoebiasis is one of the most widely distributed diseases in Ethiopia. The combination of metronidazole with Diloxanide furoate represents a new approach for the treatment of the infection. Objective This study aimed to analyze the cost-effectiveness of Diloxanide plus metronidazole compared with metronidazole alone in the treatment of amoebiasis in Ethiopia. Methods An analytical decision model was used to analyze costs and effectiveness from a societal perspective by taking adult amoebic patients as the study population with a time horizon of two months. The potential impacts of uncertainty in single parameters were explored in one-way sensitivity analyses. Results Metronidazole with Diloxanide had a higher cost and effect compared to metronidazole alone with an incremental cost-effectiveness ratio (ICER) of 8 US$ per amoebic case cured. The result was sensitive to the decrease in the effectiveness of metronidazole with Diloxanide. Conclusion This study revealed the addition of Diloxanide to standard treatment to be a more effective and more costly treatment strategy. Therefore, a decision for choosing the medication should be based on the ability of patients to pay for the treatment.
Ghada M Hadad - One of the best experts on this subject based on the ideXlab platform.
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development and optimization of hplc analysis of metronidazole Diloxanide spiramycin and cliquinol in pharmaceutical dosage forms using experimental design
Journal of Chromatographic Science, 2016Co-Authors: Mahmoud M Elkhoudary, Randa Abdel A Salam, Ghada M HadadAbstract:A new simple, sensitive, rapid and accurate gradient reversed-phase high-performance liquid chromatography with photodiode array detector (RP-HPLC-DAD) was developed and validated for simultaneous analysis of Metronidazole (MNZ), Spiramycin (SPY), Diloxanidefuroate (DIX) and Cliquinol (CLQ) using statistical experimental design. Initially, a resolution V fractional factorial design was used in order to screen five independent factors: the column temperature (°C), pH, phosphate buffer concentration (mM), flow rate (ml/min) and the initial fraction of mobile phase B (%). pH, flow rate and initial fraction of mobile phase B were identified as significant, using analysis of variance. The optimum conditions of separation determined with the aid of central composite design were: (1) initial mobile phase concentration: phosphate buffer/methanol (50/50, v/v), (2) phosphate buffer concentration (50 mM), (3) pH (4.72), (4) column temperature 30°C and (5) mobile phase flow rate (0.8 ml min-1). Excellent linearity was observed for all of the standard calibration curves, and the correlation coefficients were above 0.9999. Limits of detection for all of the analyzed compounds ranged between 0.02 and 0.11 μg ml-1; limits of quantitation ranged between 0.06 and 0.33 μg ml-1 The proposed method showed good prediction ability. The optimized method was validated according to ICH guidelines. Three commercially available tablets were analyzed showing good % recovery and %RSD.
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comparative artificial neural network and partial least squares models for analysis of metronidazole Diloxanide spiramycin and cliquinol in pharmaceutical preparations
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2014Co-Authors: Mahmoud M Elkhoudary, Randa Abdel A Salam, Ghada M HadadAbstract:Metronidazole (MNZ) is a widely used antibacterial and amoebicide drug. Therefore, it is important to develop a rapid and specific analytical method for the determination of MNZ in mixture with Spiramycin (SPY), Diloxanide (DIX) and Cliquinol (CLQ) in pharmaceutical preparations. This work describes simple, sensitive and reliable six multivariate calibration methods, namely linear and nonlinear artificial neural networks preceded by genetic algorithm (GA-ANN) and principle component analysis (PCA-ANN) as well as partial least squares (PLS) either alone or preceded by genetic algorithm (GA-PLS) for UV spectrophotometric determination of MNZ, SPY, DIX and CLQ in pharmaceutical preparations with no interference of pharmaceutical additives. The results manifest the problem of nonlinearity and how models like ANN can handle it. Analytical performance of these methods was statistically validated with respect to linearity, accuracy, precision and specificity. The developed methods indicate the ability of the previously mentioned multivariate calibration models to handle and solve UV spectra of the four components' mixtures using easy and widely used UV spectrophotometer.
Nanati Legese - One of the best experts on this subject based on the ideXlab platform.
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cost effectiveness analysis of metronidazole versus metronidazole with Diloxanide furoate in the treatment of amoebiasis in ethiopia
ClinicoEconomics and Outcomes Research, 2021Co-Authors: Nanati Legese, Temesgen Aferu, Tsehay KassaAbstract:Introduction Amoebiasis is one of the world's most prevalent and fatal infectious diseases. Several surveys revealed that amoebiasis is one of the most widely distributed diseases in Ethiopia. The combination of metronidazole with Diloxanide furoate represents a new approach for the treatment of the infection. Objective This study aimed to analyze the cost-effectiveness of Diloxanide plus metronidazole compared with metronidazole alone in the treatment of amoebiasis in Ethiopia. Methods An analytical decision model was used to analyze costs and effectiveness from a societal perspective by taking adult amoebic patients as the study population with a time horizon of two months. The potential impacts of uncertainty in single parameters were explored in one-way sensitivity analyses. Results Metronidazole with Diloxanide had a higher cost and effect compared to metronidazole alone with an incremental cost-effectiveness ratio (ICER) of 8 US$ per amoebic case cured. The result was sensitive to the decrease in the effectiveness of metronidazole with Diloxanide. Conclusion This study revealed the addition of Diloxanide to standard treatment to be a more effective and more costly treatment strategy. Therefore, a decision for choosing the medication should be based on the ability of patients to pay for the treatment.
Nour E Wagieh - One of the best experts on this subject based on the ideXlab platform.
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simultaneous determination of Diloxanide furoate and metronidazole in presence of Diloxanide furoate degradation products
Journal of AOAC International, 2011Co-Authors: Samah S Abbas, Nour E Wagieh, Mohamed Abdelkawy, Maha M AbdelrahmanAbstract:Three methods are presented for the simultaneous determination of Diloxanide furoate (DLX) and metronidazole (MTR), used for their antiprotozoal and antiamoebic effect, in the presence of DLX alkaline degradates and in pharmaceutical formulations, without previous separation. The first method is chemometric-assisted spectrophotometry, in which principal component regression and partial least squares were applied. These two approaches were successfully applied to quantify each drug in the mixture using the information included in, the absorption spectra in the range of 225-320 nm. The second method is TLC-densitometry, in which the binary mixture and degradates were separated on silica gel plates using a chloroform-acetone-glacial acetic acid (9.5 + 0.5 + 0.07, v/v/v) mobile phase and the bands were scanned at 254 nm. The last method is HPLC, in which DLX, MTR, and degradates were separated using the mobile phase acetonitrile-0.05 M dibasic potassium phosphate (25 + 75, v/v), adjusted to pH 4 with orthophosphoric acid, at a flow rate of 1 mL/min, on a C18 analytical column. Detection was at 254 nm. The proposed methods were successfully applied for the analysis of DLX and MTR in pharmaceutical formulations, and the results were statistically compared with a reported spectrophotometric method.
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stability indicating methods for the determination of Diloxanide furoate
Journal of Pharmaceutical and Biomedical Analysis, 2002Co-Authors: Nagiba Y Hasan, Mohamed A Elkawy, Badr E Elzeany, Nour E WagiehAbstract:Abstract Five new selective, precise and accurate methods are described for the determination of Diloxanide furoate (DI) in presence of its degradation products. Method A utilizes the first and second derivative spectrophotometry at 270 and 280 nm, respectively. Method B is a RSD1 spectrophotometric method based on the simultaneous use of the first derivative of ratio spectra and measurement at 270 nm. Method C is a pH-induced difference spectrophotometry using UV measurement at 295 nm. Method D is a densitometric one, after separation on silica gel plate using chloroform: methanol as mobile phase and the spots were scanned at 258 nm. Method E is reversed phase high performance liquid chromatography using methanol: water (80:20% v/v) as mobile phase at a flow rate of 1 ml/min and UV detection at 258 nm. Regression analysis showed good correlation in the concentration ranges 5–30, 5–25, 10–40 μg/ml, 100–500 ng/spot, 2–50 μg/ml with percentage recoveries of 99.92±0.56 and 99.79±0.47, 99.23±0.38, 99.96±0.06, 99.03±0.51, 98.81±0.68 for methods A, B, C, D and E, respectively. These methods are suitable as stability indicating methods for the determination of DI in presence of its degradation products either in bulk powder or in pharmaceutical formulations.
Mahmoud M Elkhoudary - One of the best experts on this subject based on the ideXlab platform.
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development and optimization of hplc analysis of metronidazole Diloxanide spiramycin and cliquinol in pharmaceutical dosage forms using experimental design
Journal of Chromatographic Science, 2016Co-Authors: Mahmoud M Elkhoudary, Randa Abdel A Salam, Ghada M HadadAbstract:A new simple, sensitive, rapid and accurate gradient reversed-phase high-performance liquid chromatography with photodiode array detector (RP-HPLC-DAD) was developed and validated for simultaneous analysis of Metronidazole (MNZ), Spiramycin (SPY), Diloxanidefuroate (DIX) and Cliquinol (CLQ) using statistical experimental design. Initially, a resolution V fractional factorial design was used in order to screen five independent factors: the column temperature (°C), pH, phosphate buffer concentration (mM), flow rate (ml/min) and the initial fraction of mobile phase B (%). pH, flow rate and initial fraction of mobile phase B were identified as significant, using analysis of variance. The optimum conditions of separation determined with the aid of central composite design were: (1) initial mobile phase concentration: phosphate buffer/methanol (50/50, v/v), (2) phosphate buffer concentration (50 mM), (3) pH (4.72), (4) column temperature 30°C and (5) mobile phase flow rate (0.8 ml min-1). Excellent linearity was observed for all of the standard calibration curves, and the correlation coefficients were above 0.9999. Limits of detection for all of the analyzed compounds ranged between 0.02 and 0.11 μg ml-1; limits of quantitation ranged between 0.06 and 0.33 μg ml-1 The proposed method showed good prediction ability. The optimized method was validated according to ICH guidelines. Three commercially available tablets were analyzed showing good % recovery and %RSD.
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comparative artificial neural network and partial least squares models for analysis of metronidazole Diloxanide spiramycin and cliquinol in pharmaceutical preparations
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2014Co-Authors: Mahmoud M Elkhoudary, Randa Abdel A Salam, Ghada M HadadAbstract:Metronidazole (MNZ) is a widely used antibacterial and amoebicide drug. Therefore, it is important to develop a rapid and specific analytical method for the determination of MNZ in mixture with Spiramycin (SPY), Diloxanide (DIX) and Cliquinol (CLQ) in pharmaceutical preparations. This work describes simple, sensitive and reliable six multivariate calibration methods, namely linear and nonlinear artificial neural networks preceded by genetic algorithm (GA-ANN) and principle component analysis (PCA-ANN) as well as partial least squares (PLS) either alone or preceded by genetic algorithm (GA-PLS) for UV spectrophotometric determination of MNZ, SPY, DIX and CLQ in pharmaceutical preparations with no interference of pharmaceutical additives. The results manifest the problem of nonlinearity and how models like ANN can handle it. Analytical performance of these methods was statistically validated with respect to linearity, accuracy, precision and specificity. The developed methods indicate the ability of the previously mentioned multivariate calibration models to handle and solve UV spectra of the four components' mixtures using easy and widely used UV spectrophotometer.