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Saeed Ahmad - One of the best experts on this subject based on the ideXlab platform.
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synthesis x ray structures and antibacterial activities of silver i complexes of 1 3 bis diphenylphosphano propane dppp and n n Dimethylthiourea dmtu
Polyhedron, 2020Co-Authors: Saeed Ahmad, Anvarhusein A. Isab, Mohammed Fettouhi, Muhammad Aziz Choudhary, Muhammad Nawaz Tahir, Muhammad Waqar Aslam, Mshari A Alotaibi, Abdulrahman I AlharthiAbstract:Abstract Three silver(I) complexes, [Ag(Dppp)2]NO3·0.5H2O (1), [Ag2(Dppp)2(Dmtu)4](NO3)2 (2) and [Ag(PPh3)2(Dmtu)2]NO3·0.5H2O (3) (where Dppp = 1,3-bis(diphenylphosphano)propane, PPh3 = triphenylphosphane and Dmtu = N,N′-Dimethylthiourea), were prepared and characterized by IR and NMR (1H, 13C and 31P) spectroscopic methods. The crystal structures of the complexes were determined by X-ray crystallography. All three complexes are ionic, containing nitrate as a counter ion. In complex 1, two Dppp ligands are coordinated to an Ag(I) ion, each acting as a chelating donor to form a six membered ring. Complex 2 is dinuclear with the individual silver(I) ions bound to two phosphorus atoms of two different Dppp ligands and two sulfur atoms of N,N′-Dimethylthiourea. The geometry around the Ag(I) atoms in both complexes is distorted tetrahedral. Complex 3 is mononuclear having the silver(I) ion coordinated by two PPh3 and two Dmtu ligands in a nearly tetrahedral environment. The Dmtu ligands in complexes 2 and 3 are coordinated in a terminal mode. The complexes were evaluated for their antibacterial activity and they were found to exhibit moderate activity against most of the bacteria.
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Synthesis, X-ray structures and antibacterial activities of silver(I) complexes of 1,3-bis(diphenylphosphano)propane (Dppp) and N,N′-Dimethylthiourea (Dmtu)
Polyhedron, 2019Co-Authors: Seerat-ur-rehman, Saeed Ahmad, Anvarhusein A. Isab, Mohammed Fettouhi, Muhammad Aziz Choudhary, Muhammad Nawaz Tahir, Muhammad Waqar Aslam, Mshari A Alotaibi, Abdulrahman I AlharthiAbstract:Abstract Three silver(I) complexes, [Ag(Dppp)2]NO3·0.5H2O (1), [Ag2(Dppp)2(Dmtu)4](NO3)2 (2) and [Ag(PPh3)2(Dmtu)2]NO3·0.5H2O (3) (where Dppp = 1,3-bis(diphenylphosphano)propane, PPh3 = triphenylphosphane and Dmtu = N,N′-Dimethylthiourea), were prepared and characterized by IR and NMR (1H, 13C and 31P) spectroscopic methods. The crystal structures of the complexes were determined by X-ray crystallography. All three complexes are ionic, containing nitrate as a counter ion. In complex 1, two Dppp ligands are coordinated to an Ag(I) ion, each acting as a chelating donor to form a six membered ring. Complex 2 is dinuclear with the individual silver(I) ions bound to two phosphorus atoms of two different Dppp ligands and two sulfur atoms of N,N′-Dimethylthiourea. The geometry around the Ag(I) atoms in both complexes is distorted tetrahedral. Complex 3 is mononuclear having the silver(I) ion coordinated by two PPh3 and two Dmtu ligands in a nearly tetrahedral environment. The Dmtu ligands in complexes 2 and 3 are coordinated in a terminal mode. The complexes were evaluated for their antibacterial activity and they were found to exhibit moderate activity against most of the bacteria.
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crystal structure and antimicrobial activity of a transplatin adduct of n n Dimethylthiourea trans pt nh3 2 dmtu 2 cl2
Monatshefte Fur Chemie, 2017Co-Authors: Saeed Ahmad, Anvarhusein A. Isab, Tobias Ruffer, Tanzeel Khalid, Abdul Rahman Alarfaj, Muhammad Saleem, Islam Ullah Khan, Muhammad Aziz ChoudharyAbstract:The crystal structure of a transplatin-derived complex, trans-[Pt(NH3)2(dmtu)2]Cl2 (dmtu = N,N ’ -Dimethylthiourea) was determined by X-ray crystallography and its antimicrobial activities were evaluated by minimum inhibitory concentration. The structure consists of trans-[Pt(NH3)2(dmtu)2]2+ cation and two chloride ions. In trans-[Pt(NH3)2(dmtu)2]2+, the geometry at platinum is nearly regular square planar with the average cis and trans angles of 90 and 178.6°, respectively. The 1H and 13C NMR spectral data indicated the coordination of both ligands to platinum(II). The results of antimicrobial studies showed that the complex exhibited significant activity against gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), while relatively poor activities were observed against molds (Aspergillus niger, Penicillium citrinum) and yeasts (Candida albicans, Saccharomyces cerevisiae).
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Crystal structure and antimicrobial activity of a transplatin adduct of N,N′-Dimethylthiourea, trans-[Pt(NH3)2(dmtu)2]Cl2
Monatshefte Fur Chemie, 2016Co-Authors: Saeed Ahmad, Anvarhusein A. Isab, Tobias Ruffer, Seerat-ur-rehman, Tanzeel Khalid, Muhammad Saleem, Islam Ullah Khan, Abdul Rahman Al-arfaj, Ejaz, Muhammad Aziz ChoudharyAbstract:The crystal structure of a transplatin-derived complex, trans-[Pt(NH3)2(dmtu)2]Cl2 (dmtu = N,N ’ -Dimethylthiourea) was determined by X-ray crystallography and its antimicrobial activities were evaluated by minimum inhibitory concentration. The structure consists of trans-[Pt(NH3)2(dmtu)2]2+ cation and two chloride ions. In trans-[Pt(NH3)2(dmtu)2]2+, the geometry at platinum is nearly regular square planar with the average cis and trans angles of 90 and 178.6°, respectively. The 1H and 13C NMR spectral data indicated the coordination of both ligands to platinum(II). The results of antimicrobial studies showed that the complex exhibited significant activity against gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), while relatively poor activities were observed against molds (Aspergillus niger, Penicillium citrinum) and yeasts (Candida albicans, Saccharomyces cerevisiae).
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Synthesis and Structural Characterization of Dibromidobis(N,N′-Dimethylthiourea-κS)cadmium(II) and Diiodidobis(N,N′-Dimethylthiourea-κS)cadmium(II)
Journal of Chemical Crystallography, 2011Co-Authors: Saeed Ahmad, Muhammad Altaf, Helen Stoeckli-evans, Anvarhusein A. Isab, Muhammam Riaz Malik, Shaukat ShujaAbstract:Cadmium(II) complexes, dibromidobis( N,N ′-Dimethylthiourea- S )cadmium(II), [Cd(Dmtu)_2Br_2] ( 1 ) and diiodidobis( N,N ′-Dimethylthiourea- S )cadmium(II), [Cd(Dmtu)_2I_2] ( 2 ), have been prepared and their structures have been determined by X-ray crystal structure analysis. Compound 1 crystallized in the monoclinic space group C 2/ c , and the metal ion is situated on a twofold rotation axis. Compound 2 also crystallized in a monoclinic space group, P 2_1/ c , but here the molecules have no crystallographic symmetry. In both compounds the cadmium atom is bonded to two halide ions and to two Dimethylthiourea molecules through the sulfur atoms in a tetrahedral environment. The molecules are linked via N–H⋯Halide hydrogen bonds to form infinite one-dimensional chains in 1 and infinite two dimensional networks in 2 . The complexes were also characterized by IR and NMR spectroscopy and the data are consistent with the structures of the compounds. Graphical Abstract Cadmium(II) complexes, dibromidobis( N,N ′-Dimethylthiourea- S )cadmium(II), [Cd(Dmtu)_2Br_2] ( 1 ) and diiodidobis( N,N ′-Dimethylthiourea- S )cadmium(II), [Cd(Dmtu)_2I_2] ( 2 ), have been prepared and their structures have been determined by X-ray crystal structure analysis. In both compounds the cadmium atom is bonded to two halide ions and to two Dimethylthiourea molecules through the sulfur atoms in a tetrahedral environment. The molecules are linked via N–H⋯Halide hydrogen bonds to form infinite one-dimensional chains in 1 and infinite two dimensional networks in 2 . The complexes were also characterized by IR and NMR spectroscopy and the data are consistent with the structures of the compounds.
M De Agazio - One of the best experts on this subject based on the ideXlab platform.
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Dimethylthiourea a hydrogen peroxide trap partially prevents stress effects and ascorbate peroxidase increase in spermidine treated maize roots
Plant Cell and Environment, 2001Co-Authors: M De Zacchini, M De AgazioAbstract:Inhibition of root growth and accumulation of putrescine caused by exogenous spermidine in roots of maize seedlings (Zea mays L., cv Samodek) were partially prevented by a concomitant treatment with Dimethylthiourea (DMTU), that traps H2O2 produced from spermidine by the activity of polyamine oxidase (PAO) in the apoplast. Treatment with spermidine caused a strong increase of ascorbate peroxidase (APX) gene expression, that was induced to a lesser extent by removing spermidine-generated H2O2 by DMTU. Over-expression of APX was associated with increased APX activity in spermidine-treated seedlings whereas the addition of DMTU to spermidine completely prevented spermidine-induced increase of APX activity. Thus, DMTU permitted the demonstration that exogenous spermidine supplied to maize seedlings causes an oxidative stress and induces APX, a key enzyme of the antioxidant defence mechanism, through H2O2, a spermidine catabolic product.
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Dimethylthiourea, a hydrogen peroxide trap, partially prevents stress effects and ascorbate peroxidase increase in spermidine‐treated maize roots
Plant Cell and Environment, 2001Co-Authors: M De Zacchini, M De AgazioAbstract:Inhibition of root growth and accumulation of putrescine caused by exogenous spermidine in roots of maize seedlings (Zea mays L., cv Samodek) were partially prevented by a concomitant treatment with Dimethylthiourea (DMTU), that traps H2O2 produced from spermidine by the activity of polyamine oxidase (PAO) in the apoplast. Treatment with spermidine caused a strong increase of ascorbate peroxidase (APX) gene expression, that was induced to a lesser extent by removing spermidine-generated H2O2 by DMTU. Over-expression of APX was associated with increased APX activity in spermidine-treated seedlings whereas the addition of DMTU to spermidine completely prevented spermidine-induced increase of APX activity. Thus, DMTU permitted the demonstration that exogenous spermidine supplied to maize seedlings causes an oxidative stress and induces APX, a key enzyme of the antioxidant defence mechanism, through H2O2, a spermidine catabolic product.
Anvarhusein A. Isab - One of the best experts on this subject based on the ideXlab platform.
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synthesis x ray structures and antibacterial activities of silver i complexes of 1 3 bis diphenylphosphano propane dppp and n n Dimethylthiourea dmtu
Polyhedron, 2020Co-Authors: Saeed Ahmad, Anvarhusein A. Isab, Mohammed Fettouhi, Muhammad Aziz Choudhary, Muhammad Nawaz Tahir, Muhammad Waqar Aslam, Mshari A Alotaibi, Abdulrahman I AlharthiAbstract:Abstract Three silver(I) complexes, [Ag(Dppp)2]NO3·0.5H2O (1), [Ag2(Dppp)2(Dmtu)4](NO3)2 (2) and [Ag(PPh3)2(Dmtu)2]NO3·0.5H2O (3) (where Dppp = 1,3-bis(diphenylphosphano)propane, PPh3 = triphenylphosphane and Dmtu = N,N′-Dimethylthiourea), were prepared and characterized by IR and NMR (1H, 13C and 31P) spectroscopic methods. The crystal structures of the complexes were determined by X-ray crystallography. All three complexes are ionic, containing nitrate as a counter ion. In complex 1, two Dppp ligands are coordinated to an Ag(I) ion, each acting as a chelating donor to form a six membered ring. Complex 2 is dinuclear with the individual silver(I) ions bound to two phosphorus atoms of two different Dppp ligands and two sulfur atoms of N,N′-Dimethylthiourea. The geometry around the Ag(I) atoms in both complexes is distorted tetrahedral. Complex 3 is mononuclear having the silver(I) ion coordinated by two PPh3 and two Dmtu ligands in a nearly tetrahedral environment. The Dmtu ligands in complexes 2 and 3 are coordinated in a terminal mode. The complexes were evaluated for their antibacterial activity and they were found to exhibit moderate activity against most of the bacteria.
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Synthesis, X-ray structures and antibacterial activities of silver(I) complexes of 1,3-bis(diphenylphosphano)propane (Dppp) and N,N′-Dimethylthiourea (Dmtu)
Polyhedron, 2019Co-Authors: Seerat-ur-rehman, Saeed Ahmad, Anvarhusein A. Isab, Mohammed Fettouhi, Muhammad Aziz Choudhary, Muhammad Nawaz Tahir, Muhammad Waqar Aslam, Mshari A Alotaibi, Abdulrahman I AlharthiAbstract:Abstract Three silver(I) complexes, [Ag(Dppp)2]NO3·0.5H2O (1), [Ag2(Dppp)2(Dmtu)4](NO3)2 (2) and [Ag(PPh3)2(Dmtu)2]NO3·0.5H2O (3) (where Dppp = 1,3-bis(diphenylphosphano)propane, PPh3 = triphenylphosphane and Dmtu = N,N′-Dimethylthiourea), were prepared and characterized by IR and NMR (1H, 13C and 31P) spectroscopic methods. The crystal structures of the complexes were determined by X-ray crystallography. All three complexes are ionic, containing nitrate as a counter ion. In complex 1, two Dppp ligands are coordinated to an Ag(I) ion, each acting as a chelating donor to form a six membered ring. Complex 2 is dinuclear with the individual silver(I) ions bound to two phosphorus atoms of two different Dppp ligands and two sulfur atoms of N,N′-Dimethylthiourea. The geometry around the Ag(I) atoms in both complexes is distorted tetrahedral. Complex 3 is mononuclear having the silver(I) ion coordinated by two PPh3 and two Dmtu ligands in a nearly tetrahedral environment. The Dmtu ligands in complexes 2 and 3 are coordinated in a terminal mode. The complexes were evaluated for their antibacterial activity and they were found to exhibit moderate activity against most of the bacteria.
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crystal structure and antimicrobial activity of a transplatin adduct of n n Dimethylthiourea trans pt nh3 2 dmtu 2 cl2
Monatshefte Fur Chemie, 2017Co-Authors: Saeed Ahmad, Anvarhusein A. Isab, Tobias Ruffer, Tanzeel Khalid, Abdul Rahman Alarfaj, Muhammad Saleem, Islam Ullah Khan, Muhammad Aziz ChoudharyAbstract:The crystal structure of a transplatin-derived complex, trans-[Pt(NH3)2(dmtu)2]Cl2 (dmtu = N,N ’ -Dimethylthiourea) was determined by X-ray crystallography and its antimicrobial activities were evaluated by minimum inhibitory concentration. The structure consists of trans-[Pt(NH3)2(dmtu)2]2+ cation and two chloride ions. In trans-[Pt(NH3)2(dmtu)2]2+, the geometry at platinum is nearly regular square planar with the average cis and trans angles of 90 and 178.6°, respectively. The 1H and 13C NMR spectral data indicated the coordination of both ligands to platinum(II). The results of antimicrobial studies showed that the complex exhibited significant activity against gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), while relatively poor activities were observed against molds (Aspergillus niger, Penicillium citrinum) and yeasts (Candida albicans, Saccharomyces cerevisiae).
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Crystal structure and antimicrobial activity of a transplatin adduct of N,N′-Dimethylthiourea, trans-[Pt(NH3)2(dmtu)2]Cl2
Monatshefte Fur Chemie, 2016Co-Authors: Saeed Ahmad, Anvarhusein A. Isab, Tobias Ruffer, Seerat-ur-rehman, Tanzeel Khalid, Muhammad Saleem, Islam Ullah Khan, Abdul Rahman Al-arfaj, Ejaz, Muhammad Aziz ChoudharyAbstract:The crystal structure of a transplatin-derived complex, trans-[Pt(NH3)2(dmtu)2]Cl2 (dmtu = N,N ’ -Dimethylthiourea) was determined by X-ray crystallography and its antimicrobial activities were evaluated by minimum inhibitory concentration. The structure consists of trans-[Pt(NH3)2(dmtu)2]2+ cation and two chloride ions. In trans-[Pt(NH3)2(dmtu)2]2+, the geometry at platinum is nearly regular square planar with the average cis and trans angles of 90 and 178.6°, respectively. The 1H and 13C NMR spectral data indicated the coordination of both ligands to platinum(II). The results of antimicrobial studies showed that the complex exhibited significant activity against gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), while relatively poor activities were observed against molds (Aspergillus niger, Penicillium citrinum) and yeasts (Candida albicans, Saccharomyces cerevisiae).
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structural characterization of dicyanidobis n n Dimethylthiourea κs cadmium ii
Journal of Structural Chemistry, 2013Co-Authors: Muhammad Riaz Malik, Anvarhusein A. Isab, Tobias Ruffer, Heinrich Lang, Sajjad Ahmad, Helen StoecklievansAbstract:A cadmium(II) complex dicyanidobis(N,N′-Dimethylthiourea-S) cadmium(II) [Cd(Dmtu)2(CN)2] (1) is prepared and its structure in the solid state is determined by single crystal X-ray structural analysis. The cadmium(II) ion is four-coordinate having a distorted tetrahedral geometry composed of two cyanide C atoms and two thione S atoms of N,N′-Dimethylthiourea (dmtu). The molecular structure is stabilized by intermolecular N-H…N(CN) hydrogen bonding interactions that lead to a 3D network structure. The complex was also characterized by IR and NMR spectroscopy.
Muhammad Aziz Choudhary - One of the best experts on this subject based on the ideXlab platform.
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synthesis x ray structures and antibacterial activities of silver i complexes of 1 3 bis diphenylphosphano propane dppp and n n Dimethylthiourea dmtu
Polyhedron, 2020Co-Authors: Saeed Ahmad, Anvarhusein A. Isab, Mohammed Fettouhi, Muhammad Aziz Choudhary, Muhammad Nawaz Tahir, Muhammad Waqar Aslam, Mshari A Alotaibi, Abdulrahman I AlharthiAbstract:Abstract Three silver(I) complexes, [Ag(Dppp)2]NO3·0.5H2O (1), [Ag2(Dppp)2(Dmtu)4](NO3)2 (2) and [Ag(PPh3)2(Dmtu)2]NO3·0.5H2O (3) (where Dppp = 1,3-bis(diphenylphosphano)propane, PPh3 = triphenylphosphane and Dmtu = N,N′-Dimethylthiourea), were prepared and characterized by IR and NMR (1H, 13C and 31P) spectroscopic methods. The crystal structures of the complexes were determined by X-ray crystallography. All three complexes are ionic, containing nitrate as a counter ion. In complex 1, two Dppp ligands are coordinated to an Ag(I) ion, each acting as a chelating donor to form a six membered ring. Complex 2 is dinuclear with the individual silver(I) ions bound to two phosphorus atoms of two different Dppp ligands and two sulfur atoms of N,N′-Dimethylthiourea. The geometry around the Ag(I) atoms in both complexes is distorted tetrahedral. Complex 3 is mononuclear having the silver(I) ion coordinated by two PPh3 and two Dmtu ligands in a nearly tetrahedral environment. The Dmtu ligands in complexes 2 and 3 are coordinated in a terminal mode. The complexes were evaluated for their antibacterial activity and they were found to exhibit moderate activity against most of the bacteria.
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Synthesis, X-ray structures and antibacterial activities of silver(I) complexes of 1,3-bis(diphenylphosphano)propane (Dppp) and N,N′-Dimethylthiourea (Dmtu)
Polyhedron, 2019Co-Authors: Seerat-ur-rehman, Saeed Ahmad, Anvarhusein A. Isab, Mohammed Fettouhi, Muhammad Aziz Choudhary, Muhammad Nawaz Tahir, Muhammad Waqar Aslam, Mshari A Alotaibi, Abdulrahman I AlharthiAbstract:Abstract Three silver(I) complexes, [Ag(Dppp)2]NO3·0.5H2O (1), [Ag2(Dppp)2(Dmtu)4](NO3)2 (2) and [Ag(PPh3)2(Dmtu)2]NO3·0.5H2O (3) (where Dppp = 1,3-bis(diphenylphosphano)propane, PPh3 = triphenylphosphane and Dmtu = N,N′-Dimethylthiourea), were prepared and characterized by IR and NMR (1H, 13C and 31P) spectroscopic methods. The crystal structures of the complexes were determined by X-ray crystallography. All three complexes are ionic, containing nitrate as a counter ion. In complex 1, two Dppp ligands are coordinated to an Ag(I) ion, each acting as a chelating donor to form a six membered ring. Complex 2 is dinuclear with the individual silver(I) ions bound to two phosphorus atoms of two different Dppp ligands and two sulfur atoms of N,N′-Dimethylthiourea. The geometry around the Ag(I) atoms in both complexes is distorted tetrahedral. Complex 3 is mononuclear having the silver(I) ion coordinated by two PPh3 and two Dmtu ligands in a nearly tetrahedral environment. The Dmtu ligands in complexes 2 and 3 are coordinated in a terminal mode. The complexes were evaluated for their antibacterial activity and they were found to exhibit moderate activity against most of the bacteria.
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crystal structure and antimicrobial activity of a transplatin adduct of n n Dimethylthiourea trans pt nh3 2 dmtu 2 cl2
Monatshefte Fur Chemie, 2017Co-Authors: Saeed Ahmad, Anvarhusein A. Isab, Tobias Ruffer, Tanzeel Khalid, Abdul Rahman Alarfaj, Muhammad Saleem, Islam Ullah Khan, Muhammad Aziz ChoudharyAbstract:The crystal structure of a transplatin-derived complex, trans-[Pt(NH3)2(dmtu)2]Cl2 (dmtu = N,N ’ -Dimethylthiourea) was determined by X-ray crystallography and its antimicrobial activities were evaluated by minimum inhibitory concentration. The structure consists of trans-[Pt(NH3)2(dmtu)2]2+ cation and two chloride ions. In trans-[Pt(NH3)2(dmtu)2]2+, the geometry at platinum is nearly regular square planar with the average cis and trans angles of 90 and 178.6°, respectively. The 1H and 13C NMR spectral data indicated the coordination of both ligands to platinum(II). The results of antimicrobial studies showed that the complex exhibited significant activity against gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), while relatively poor activities were observed against molds (Aspergillus niger, Penicillium citrinum) and yeasts (Candida albicans, Saccharomyces cerevisiae).
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Crystal structure and antimicrobial activity of a transplatin adduct of N,N′-Dimethylthiourea, trans-[Pt(NH3)2(dmtu)2]Cl2
Monatshefte Fur Chemie, 2016Co-Authors: Saeed Ahmad, Anvarhusein A. Isab, Tobias Ruffer, Seerat-ur-rehman, Tanzeel Khalid, Muhammad Saleem, Islam Ullah Khan, Abdul Rahman Al-arfaj, Ejaz, Muhammad Aziz ChoudharyAbstract:The crystal structure of a transplatin-derived complex, trans-[Pt(NH3)2(dmtu)2]Cl2 (dmtu = N,N ’ -Dimethylthiourea) was determined by X-ray crystallography and its antimicrobial activities were evaluated by minimum inhibitory concentration. The structure consists of trans-[Pt(NH3)2(dmtu)2]2+ cation and two chloride ions. In trans-[Pt(NH3)2(dmtu)2]2+, the geometry at platinum is nearly regular square planar with the average cis and trans angles of 90 and 178.6°, respectively. The 1H and 13C NMR spectral data indicated the coordination of both ligands to platinum(II). The results of antimicrobial studies showed that the complex exhibited significant activity against gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), while relatively poor activities were observed against molds (Aspergillus niger, Penicillium citrinum) and yeasts (Candida albicans, Saccharomyces cerevisiae).
M De Zacchini - One of the best experts on this subject based on the ideXlab platform.
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Dimethylthiourea a hydrogen peroxide trap partially prevents stress effects and ascorbate peroxidase increase in spermidine treated maize roots
Plant Cell and Environment, 2001Co-Authors: M De Zacchini, M De AgazioAbstract:Inhibition of root growth and accumulation of putrescine caused by exogenous spermidine in roots of maize seedlings (Zea mays L., cv Samodek) were partially prevented by a concomitant treatment with Dimethylthiourea (DMTU), that traps H2O2 produced from spermidine by the activity of polyamine oxidase (PAO) in the apoplast. Treatment with spermidine caused a strong increase of ascorbate peroxidase (APX) gene expression, that was induced to a lesser extent by removing spermidine-generated H2O2 by DMTU. Over-expression of APX was associated with increased APX activity in spermidine-treated seedlings whereas the addition of DMTU to spermidine completely prevented spermidine-induced increase of APX activity. Thus, DMTU permitted the demonstration that exogenous spermidine supplied to maize seedlings causes an oxidative stress and induces APX, a key enzyme of the antioxidant defence mechanism, through H2O2, a spermidine catabolic product.
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Dimethylthiourea, a hydrogen peroxide trap, partially prevents stress effects and ascorbate peroxidase increase in spermidine‐treated maize roots
Plant Cell and Environment, 2001Co-Authors: M De Zacchini, M De AgazioAbstract:Inhibition of root growth and accumulation of putrescine caused by exogenous spermidine in roots of maize seedlings (Zea mays L., cv Samodek) were partially prevented by a concomitant treatment with Dimethylthiourea (DMTU), that traps H2O2 produced from spermidine by the activity of polyamine oxidase (PAO) in the apoplast. Treatment with spermidine caused a strong increase of ascorbate peroxidase (APX) gene expression, that was induced to a lesser extent by removing spermidine-generated H2O2 by DMTU. Over-expression of APX was associated with increased APX activity in spermidine-treated seedlings whereas the addition of DMTU to spermidine completely prevented spermidine-induced increase of APX activity. Thus, DMTU permitted the demonstration that exogenous spermidine supplied to maize seedlings causes an oxidative stress and induces APX, a key enzyme of the antioxidant defence mechanism, through H2O2, a spermidine catabolic product.