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Aleksandro Schafer Da Silva - One of the best experts on this subject based on the ideXlab platform.
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Diminazene aceturate associated with sodium selenite and vitamin E in the treatment of Trypanosoma evansi infection in rats.
Experimental Parasitology, 2011Co-Authors: Alexandre A. Tonin, Aleksandro Schafer Da Silva, Márcio Machado Costa, Mateus Anderson Otto, Gustavo R. Thomé, Kaio César Simano Tavares, Luiz Claudio Miletti, Marta Lizandra Do Rêgo Leal, Sonia Terezinha Dos Anjos Lopes, Chiara Maria MazzantiAbstract:The aim of this study was to evaluate the utilization of a standard treatment with Diminazene aceturate against the infection caused by Trypanosoma evansi, associated to sodium selenite and vitamin E. In vitro tests showed trypanocidal effect related to the treatment with Diminazene aceturate and sodium selenite, but vitamin E had no harmful effect on the trypanosomes. In vivo experiments utilized a total of 72 adult outbreed females rats, separated into 9 groups (A, B, C, D, E, F, G, H and I), 8 animals each. Group A was the uninfected group; groups B to I were infected with 0.2mL of blood containing 10(6) trypanosomes. Parasitemia was estimated daily by microscopic examination of blood smears. Group B served as positive control; group C was treated with Diminazene aceturate; group D with sodium selenite; group E with vitamin E; group F received an association of Diminazene aceturate and sodium selenite; group G received an association of Diminazene aceturate and vitamin E; group H received an association of Diminazene aceturate, sodium selenite and vitamin E, and group I received an association of sodium selenite and vitamin E. Diminazene aceturate was administrated in a single dose on the 3rd day post infection (PI). Sodium selenite and vitamin E were administered at the 3rd and 23rd day PI. In vivo tests showed increase of longevity in groups treated with Diminazene aceturate associated with sodium selenite (groups F and H). No difference was found between groups C and E, thus the vitamin E did not increase the efficacy of treatment against T. evansi when associated to Diminazene aceturate. The curative efficacy of treatments was 37.5, 87.7, 37.7 and 75% to the groups C, F, G and H, respectively. Other treatments showed no efficacy. The sodium selenite when combined with chemotherapy may represent an alternative in the treatment of trypanosomosis.
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Diminazene aceturate in the control of Trypanosoma evansi infection in cats.
Veterinary Parasitology, 2009Co-Authors: Aleksandro Schafer Da Silva, Márcio Machado Costa, Sonia Terezinha Dos Anjos Lopes, Régis Adriel Zanette, Patrícia Wolkmer, Herakles A. Garcia, Janio Morais Santurio, M.m.g. TeixeiraAbstract:Abstract The aim of this study was to investigate the efficacy of Diminazene aceturate in the control of the infection by Trypanosoma evansi in cats. Fourteen animals were infected with 108 trypomastigote forms each and six were used as negative control (group A). Seven of the infected cats were used as positive control (group B) and seven were treated with Diminazene aceturate (3.5 mg kg−1) for 5 consecutive days (group C). Biochemical and hematological parameters were evaluated during the experiment. Blood with anticoagulant was collected at day 49 post-inoculation and preserved in ethanol for DNA extraction. Samples were analyzed using PCR T. evansi-specific to assess the effectiveness of treatment. The treatment with Diminazene aceturate had an efficacy of 85.7%. Alanine aminotransferase, gamma-glutamyltransferase, urea, and creatinine values remained within the normal physiological range in the treated cats. Hemogram was normalized in all the cured animals. Therefore, the therapy used is effective in controlling T. evansi in cats.
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aceturato de diminazeno e dipropionato de imidocarb no controle de infeccao por trypanosoma evansi em rattus norvegicus infectados experimentalmente
Ciencia Rural, 2008Co-Authors: Aleksandro Schafer Da Silva, Régis Adriel Zanette, Janio Morais Santurio, Camila Tochetto, Felipe Pierezan, Daniel Ricardo Rissi, Silvia Gonzalez MonteiroAbstract:The aim of this study was to evaluate the efficacy of Diminazene aceturate and imidocarb dipropionate in the control of Trypanosoma evansi infection in rats (Rattus norvegicus) experimentally infected. Fifty-four male rats were inoculated through intraperitoneal route with 104 T. evansi trypomastigotes. The rats were evaluated daily by periferic blood smears examination and treated when eight flagellated parasites were observed in 1000x microscopic field. Two therapeutics protocols were used. The first one included Groups A, B, C, D in which the rats were submitted to a single dose of the testing drugs administered by intramuscular route at the day 0 and again when T. evansi was observed in the blood smears. The rats of the second protocol (Groups E, F, G, H) were submitted to the same treatment by five consecutive days. Four rats (Group I) were used as control and were not submitted to any treatment. Tested drugs did not show any curative effect when used in the first protocol, since parasitaemia was evident few days after treatment. The use of Diminazene aceturate in the second protocol resulted in elimination of the trypomastigotes from circulation. In this case the rats were euthanized at the day 90. The infection recurred 30 days after the administration of imidocarb dipropionate. Histologically, no lesions were found in the liver or kidney. Diminazene aceturate is effective in treating trypanosomosis in rats when used five days consecutively.
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Aceturato de diminazeno e dipropionato de imidocarb no controle de infecção por Trypanosoma evansi em Rattus norvegicus infectados experimentalmente Diminazene aceturate and imidocarb dipropionate in the control of Trypanosoma evansi infection in Rat
Universidade Federal de Santa Maria, 2008Co-Authors: Aleksandro Schafer Da Silva, Régis Adriel Zanette, Janio Morais Santurio, Camila Tochetto, Felipe Pierezan, Daniel Ricardo Rissi, Silvia Gonzalez MonteiroAbstract:Este estudo teve como objetivo avaliar o efeito do aceturato de diminazeno e do dipropionato de imidocarb no controle da infecção por Trypanosoma evansi em ratos (Rattus norvegicus) infectados experimentalmente. Cinqüenta e quatro ratos machos foram inoculados via intraperitonial com 104 tripomastigotas de T. evansi/animal. Os ratos foram monitorados diariamente por meio de esfregaço sanguíneo periférico. No momento em que se observassem oito protozoários por campo microscópico de 1000x, era iniciado o tratamento com as drogas (dia zero). O estudo foi dividido em dois protocolos terapêuticos e os fármacos foram administrados via intramuscular. O primeiro protocolo foi aplicado nos grupos A, B, C e D e o segundo protocolo nos grupos E, F, G e H. O grupo controle foi identificado como grupo I, não medicados. No primeiro protocolo, os ratos receberam uma dose única dos fármacos no dia zero e sempre que se observasse T. evansi na circulação periférica. No segundo protocolo, os roedores receberam as mesmas doses, no entanto, por cinco dias consecutivos. No primeiro protocolo, os dois princípios ativos não apresentaram eficácia curativa, ocorrendo reincidência da parasitemia após alguns dias do tratamento. No segundo protocolo, o aceturato de diminazeno eliminou a forma tripomastigota da circulação e os ratos foram eutanasiados após 90 dias do início do tratamento. Os roedores tratados com dipropionato de imidocarb apresentaram recidiva da infecção após 30 dias. Na histopatologia não se observou alteração renal e hepática relacionada à doença ou aos medicamentos testados. Com base nos resultados, foi concluído que o aceturato de diminazeno, quando administrado por cinco dias consecutivos, é efetivo no tratamento da tripanossomose em ratos.The aim of this study was to evaluate the efficacy of Diminazene aceturate and imidocarb dipropionate in the control of Trypanosoma evansi infection in rats (Rattus norvegicus) experimentally infected. Fifty-four male rats were inoculated through intraperitoneal route with 104 T. evansi trypomastigotes. The rats were evaluated daily by periferic blood smears examination and treated when eight flagellated parasites were observed in 1000x microscopic field. Two therapeutics protocols were used. The first one included Groups A, B, C, D in which the rats were submitted to a single dose of the testing drugs administered by intramuscular route at the day 0 and again when T. evansi was observed in the blood smears. The rats of the second protocol (Groups E, F, G, H) were submitted to the same treatment by five consecutive days. Four rats (Group I) were used as control and were not submitted to any treatment. Tested drugs did not show any curative effect when used in the first protocol, since parasitaemia was evident few days after treatment. The use of Diminazene aceturate in the second protocol resulted in elimination of the trypomastigotes from circulation. In this case the rats were euthanized at the day 90. The infection recurred 30 days after the administration of imidocarb dipropionate. Histologically, no lesions were found in the liver or kidney. Diminazene aceturate is effective in treating trypanosomosis in rats when used five days consecutively
T Baltz - One of the best experts on this subject based on the ideXlab platform.
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in vivo and in vitro sensitivity of trypanosoma evansi and t equiperdum to Diminazene suramin melcy quinapyramine and isometamidium
Acta Tropica, 1991Co-Authors: Z Q Zhang, C Giroud, T BaltzAbstract:Abstract The sensitivity of three Trypanosoma equiperdum clones and thirteen Trypanosoma evansi clones originating from the People's Republic of China, the Philippines, Ethiopia and elsewhere to a series of drugs was determined in vivo and in vitro. The drugs tested were Diminazene aceturate (Berenil), suramin (Naganol), MelCy (Cymelarsan), quinapyramine sulfate (Trypacide) and isometamidium chloride (Samorin). The activity of each drug was expressed as: 1) in vitro: the minimal effective concentration which killed trypanosome population by 100% within 24 h of drug exposure (MEC 100 ); the maximum tolerated concentration in which trypanosomes could propagate at the same rate as the controls during 48 h of drug exposure (MTC 100 ); 2) in vivo: the curative dosage in 100% of infected mice (CD 100 ); the highest ineffective dosage: 100% of infected mice remain infected (ID 100 ). MEC 100 values of Diminazene aceturate ranged from 0.0556 μg/ml to 14.24 μg/ml for the eleven tested clones (differed by 256-fold); CD 100 values of this drug ranged from 2.25 mg/kg to > 89 mg/kg (differed by >40-fold). Diminazene aceturate at up to 89 mg/kg had no effect on T. evansi SHBR, T. equiperdum PBR (Berenil resistant organisms selected by continual passage of the organisms through mice treated with increasing concentrations of drug), or T. evansi AH (strain isolated in the field). Comparable MEC 100 values for other trypanocides tested were 1–8 μg/ml for suramin, 0.005–0.04 μg/ml for MelCy, 1–16 μg/ml for quinapyramine sulfate and 1–4 μg/ml for isometamidium chloride. Clones selected for resistance to Diminazene aceturate were not cross-resistant to suramin and isometamidium chloride. In contrast, the clones resistant to Diminazene were shown to be more sensitive to quinapyramine sulfate than the normal strains in in vivo tests. The results indicate that resistance to Diminazene aceturate by T. evansi and T. equiperdum clones in vivo also occurred in vitro. Resistance to isometamidium chloride in the clones tested in vivo was not observed in vitro, except for T. equiperdum SA. It therefore appears that drug bioavailability is altered or drug biotransformation occurs during the in vivo test. We conclude that the in vitro assay procedure may be of potential use for screening new trypanocides and also for the rapid detection of drug resistant isolates of T. evansi and T. equiperdum .
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in vivo and in vitro sensitivity of trypanosoma evansi and t equiperdum to Diminazene suramin melcy quinapyramine and isometamidium
Acta Tropica, 1991Co-Authors: Z Q Zhang, C Giroud, T BaltzAbstract:The sensitivity of three Trypanosoma equiperdum clones and thirteen Trypanosoma evansi clones originating from the People's Republic of China, the Philippines, Ethiopia and elsewhere to a series of drugs was determined in vivo and in vitro. The drugs tested were Diminazene aceturate (Berenil), suramin (Naganol), MelCy (Cymelarsan), quinapyramine sulfate (Trypacide) and isometamidium chloride (Samorin). The activity of each drug was expressed as: 1) in vitro: the minimal effective concentration which killed trypanosome population by 100% within 24 h of drug exposure (MEC100); the maximum tolerated concentration in which trypanosomes could propagate at the same rate as the controls during 48 h of drug exposure (MTC100); 2) in vivo: the curative dosage in 100% of infected mice (CD100); the highest ineffective dosage: 100% of infected mice remain infected (ID100). MEC100 values of Diminazene aceturate ranged from 0.0556 microgram/ml to 14.24 micrograms/ml for the eleven tested clones (differed by 256-fold); CD100 values of this drug ranged from 2.25 mg/kg to greater than 89 mg/kg (differed by greater than 40-fold). Diminazene aceturate at up to 89 mg/kg had no effect on T. evansi SHBR, T. equiperdum PBR (Berenil resistant organisms selected by continual passage of the organisms through mice treated with increasing concentrations of drug), or T. evansi AH (strain isolated in the field). Comparable MEC100 values for other trypanocides tested were 1-8 micrograms/ml for suramin, 0.005-0.04 microgram/ml for MelCy, 1-16 micrograms/ml for quinapyramine sulfate and 1-4 micrograms/ml for isometamidium chloride. Clones selected for resistance to Diminazene aceturate were not cross-resistant to suramin and isometamidium chloride. In contrast, the clones resistant to Diminazene were shown to be more sensitive to quinapyramine sulfate than the normal strains in in vivo tests. The results indicate that resistance to Diminazene aceturate by T. evansi and T. equiperdum clones in vivo also occurred in vitro. Resistance to isometamidium chloride in the clones tested in vivo was not observed in vitro, except for T. equiperdum SA. It therefore appears that drug bioavailability is altered or drug biotransformation occurs during the in vivo test. We conclude that the in vitro assay procedure may be of potential use for screening new trypanocides and also for the rapid detection of drug resistant isolates of T. evansi and T. equiperdum.
Régis Adriel Zanette - One of the best experts on this subject based on the ideXlab platform.
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Diminazene aceturate in the control of Trypanosoma evansi infection in cats.
Veterinary Parasitology, 2009Co-Authors: Aleksandro Schafer Da Silva, Márcio Machado Costa, Sonia Terezinha Dos Anjos Lopes, Régis Adriel Zanette, Patrícia Wolkmer, Herakles A. Garcia, Janio Morais Santurio, M.m.g. TeixeiraAbstract:Abstract The aim of this study was to investigate the efficacy of Diminazene aceturate in the control of the infection by Trypanosoma evansi in cats. Fourteen animals were infected with 108 trypomastigote forms each and six were used as negative control (group A). Seven of the infected cats were used as positive control (group B) and seven were treated with Diminazene aceturate (3.5 mg kg−1) for 5 consecutive days (group C). Biochemical and hematological parameters were evaluated during the experiment. Blood with anticoagulant was collected at day 49 post-inoculation and preserved in ethanol for DNA extraction. Samples were analyzed using PCR T. evansi-specific to assess the effectiveness of treatment. The treatment with Diminazene aceturate had an efficacy of 85.7%. Alanine aminotransferase, gamma-glutamyltransferase, urea, and creatinine values remained within the normal physiological range in the treated cats. Hemogram was normalized in all the cured animals. Therefore, the therapy used is effective in controlling T. evansi in cats.
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aceturato de diminazeno e dipropionato de imidocarb no controle de infeccao por trypanosoma evansi em rattus norvegicus infectados experimentalmente
Ciencia Rural, 2008Co-Authors: Aleksandro Schafer Da Silva, Régis Adriel Zanette, Janio Morais Santurio, Camila Tochetto, Felipe Pierezan, Daniel Ricardo Rissi, Silvia Gonzalez MonteiroAbstract:The aim of this study was to evaluate the efficacy of Diminazene aceturate and imidocarb dipropionate in the control of Trypanosoma evansi infection in rats (Rattus norvegicus) experimentally infected. Fifty-four male rats were inoculated through intraperitoneal route with 104 T. evansi trypomastigotes. The rats were evaluated daily by periferic blood smears examination and treated when eight flagellated parasites were observed in 1000x microscopic field. Two therapeutics protocols were used. The first one included Groups A, B, C, D in which the rats were submitted to a single dose of the testing drugs administered by intramuscular route at the day 0 and again when T. evansi was observed in the blood smears. The rats of the second protocol (Groups E, F, G, H) were submitted to the same treatment by five consecutive days. Four rats (Group I) were used as control and were not submitted to any treatment. Tested drugs did not show any curative effect when used in the first protocol, since parasitaemia was evident few days after treatment. The use of Diminazene aceturate in the second protocol resulted in elimination of the trypomastigotes from circulation. In this case the rats were euthanized at the day 90. The infection recurred 30 days after the administration of imidocarb dipropionate. Histologically, no lesions were found in the liver or kidney. Diminazene aceturate is effective in treating trypanosomosis in rats when used five days consecutively.
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Aceturato de diminazeno e dipropionato de imidocarb no controle de infecção por Trypanosoma evansi em Rattus norvegicus infectados experimentalmente Diminazene aceturate and imidocarb dipropionate in the control of Trypanosoma evansi infection in Rat
Universidade Federal de Santa Maria, 2008Co-Authors: Aleksandro Schafer Da Silva, Régis Adriel Zanette, Janio Morais Santurio, Camila Tochetto, Felipe Pierezan, Daniel Ricardo Rissi, Silvia Gonzalez MonteiroAbstract:Este estudo teve como objetivo avaliar o efeito do aceturato de diminazeno e do dipropionato de imidocarb no controle da infecção por Trypanosoma evansi em ratos (Rattus norvegicus) infectados experimentalmente. Cinqüenta e quatro ratos machos foram inoculados via intraperitonial com 104 tripomastigotas de T. evansi/animal. Os ratos foram monitorados diariamente por meio de esfregaço sanguíneo periférico. No momento em que se observassem oito protozoários por campo microscópico de 1000x, era iniciado o tratamento com as drogas (dia zero). O estudo foi dividido em dois protocolos terapêuticos e os fármacos foram administrados via intramuscular. O primeiro protocolo foi aplicado nos grupos A, B, C e D e o segundo protocolo nos grupos E, F, G e H. O grupo controle foi identificado como grupo I, não medicados. No primeiro protocolo, os ratos receberam uma dose única dos fármacos no dia zero e sempre que se observasse T. evansi na circulação periférica. No segundo protocolo, os roedores receberam as mesmas doses, no entanto, por cinco dias consecutivos. No primeiro protocolo, os dois princípios ativos não apresentaram eficácia curativa, ocorrendo reincidência da parasitemia após alguns dias do tratamento. No segundo protocolo, o aceturato de diminazeno eliminou a forma tripomastigota da circulação e os ratos foram eutanasiados após 90 dias do início do tratamento. Os roedores tratados com dipropionato de imidocarb apresentaram recidiva da infecção após 30 dias. Na histopatologia não se observou alteração renal e hepática relacionada à doença ou aos medicamentos testados. Com base nos resultados, foi concluído que o aceturato de diminazeno, quando administrado por cinco dias consecutivos, é efetivo no tratamento da tripanossomose em ratos.The aim of this study was to evaluate the efficacy of Diminazene aceturate and imidocarb dipropionate in the control of Trypanosoma evansi infection in rats (Rattus norvegicus) experimentally infected. Fifty-four male rats were inoculated through intraperitoneal route with 104 T. evansi trypomastigotes. The rats were evaluated daily by periferic blood smears examination and treated when eight flagellated parasites were observed in 1000x microscopic field. Two therapeutics protocols were used. The first one included Groups A, B, C, D in which the rats were submitted to a single dose of the testing drugs administered by intramuscular route at the day 0 and again when T. evansi was observed in the blood smears. The rats of the second protocol (Groups E, F, G, H) were submitted to the same treatment by five consecutive days. Four rats (Group I) were used as control and were not submitted to any treatment. Tested drugs did not show any curative effect when used in the first protocol, since parasitaemia was evident few days after treatment. The use of Diminazene aceturate in the second protocol resulted in elimination of the trypomastigotes from circulation. In this case the rats were euthanized at the day 90. The infection recurred 30 days after the administration of imidocarb dipropionate. Histologically, no lesions were found in the liver or kidney. Diminazene aceturate is effective in treating trypanosomosis in rats when used five days consecutively
Janio Morais Santurio - One of the best experts on this subject based on the ideXlab platform.
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Diminazene aceturate in the control of Trypanosoma evansi infection in cats.
Veterinary Parasitology, 2009Co-Authors: Aleksandro Schafer Da Silva, Márcio Machado Costa, Sonia Terezinha Dos Anjos Lopes, Régis Adriel Zanette, Patrícia Wolkmer, Herakles A. Garcia, Janio Morais Santurio, M.m.g. TeixeiraAbstract:Abstract The aim of this study was to investigate the efficacy of Diminazene aceturate in the control of the infection by Trypanosoma evansi in cats. Fourteen animals were infected with 108 trypomastigote forms each and six were used as negative control (group A). Seven of the infected cats were used as positive control (group B) and seven were treated with Diminazene aceturate (3.5 mg kg−1) for 5 consecutive days (group C). Biochemical and hematological parameters were evaluated during the experiment. Blood with anticoagulant was collected at day 49 post-inoculation and preserved in ethanol for DNA extraction. Samples were analyzed using PCR T. evansi-specific to assess the effectiveness of treatment. The treatment with Diminazene aceturate had an efficacy of 85.7%. Alanine aminotransferase, gamma-glutamyltransferase, urea, and creatinine values remained within the normal physiological range in the treated cats. Hemogram was normalized in all the cured animals. Therefore, the therapy used is effective in controlling T. evansi in cats.
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aceturato de diminazeno e dipropionato de imidocarb no controle de infeccao por trypanosoma evansi em rattus norvegicus infectados experimentalmente
Ciencia Rural, 2008Co-Authors: Aleksandro Schafer Da Silva, Régis Adriel Zanette, Janio Morais Santurio, Camila Tochetto, Felipe Pierezan, Daniel Ricardo Rissi, Silvia Gonzalez MonteiroAbstract:The aim of this study was to evaluate the efficacy of Diminazene aceturate and imidocarb dipropionate in the control of Trypanosoma evansi infection in rats (Rattus norvegicus) experimentally infected. Fifty-four male rats were inoculated through intraperitoneal route with 104 T. evansi trypomastigotes. The rats were evaluated daily by periferic blood smears examination and treated when eight flagellated parasites were observed in 1000x microscopic field. Two therapeutics protocols were used. The first one included Groups A, B, C, D in which the rats were submitted to a single dose of the testing drugs administered by intramuscular route at the day 0 and again when T. evansi was observed in the blood smears. The rats of the second protocol (Groups E, F, G, H) were submitted to the same treatment by five consecutive days. Four rats (Group I) were used as control and were not submitted to any treatment. Tested drugs did not show any curative effect when used in the first protocol, since parasitaemia was evident few days after treatment. The use of Diminazene aceturate in the second protocol resulted in elimination of the trypomastigotes from circulation. In this case the rats were euthanized at the day 90. The infection recurred 30 days after the administration of imidocarb dipropionate. Histologically, no lesions were found in the liver or kidney. Diminazene aceturate is effective in treating trypanosomosis in rats when used five days consecutively.
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Aceturato de diminazeno e dipropionato de imidocarb no controle de infecção por Trypanosoma evansi em Rattus norvegicus infectados experimentalmente Diminazene aceturate and imidocarb dipropionate in the control of Trypanosoma evansi infection in Rat
Universidade Federal de Santa Maria, 2008Co-Authors: Aleksandro Schafer Da Silva, Régis Adriel Zanette, Janio Morais Santurio, Camila Tochetto, Felipe Pierezan, Daniel Ricardo Rissi, Silvia Gonzalez MonteiroAbstract:Este estudo teve como objetivo avaliar o efeito do aceturato de diminazeno e do dipropionato de imidocarb no controle da infecção por Trypanosoma evansi em ratos (Rattus norvegicus) infectados experimentalmente. Cinqüenta e quatro ratos machos foram inoculados via intraperitonial com 104 tripomastigotas de T. evansi/animal. Os ratos foram monitorados diariamente por meio de esfregaço sanguíneo periférico. No momento em que se observassem oito protozoários por campo microscópico de 1000x, era iniciado o tratamento com as drogas (dia zero). O estudo foi dividido em dois protocolos terapêuticos e os fármacos foram administrados via intramuscular. O primeiro protocolo foi aplicado nos grupos A, B, C e D e o segundo protocolo nos grupos E, F, G e H. O grupo controle foi identificado como grupo I, não medicados. No primeiro protocolo, os ratos receberam uma dose única dos fármacos no dia zero e sempre que se observasse T. evansi na circulação periférica. No segundo protocolo, os roedores receberam as mesmas doses, no entanto, por cinco dias consecutivos. No primeiro protocolo, os dois princípios ativos não apresentaram eficácia curativa, ocorrendo reincidência da parasitemia após alguns dias do tratamento. No segundo protocolo, o aceturato de diminazeno eliminou a forma tripomastigota da circulação e os ratos foram eutanasiados após 90 dias do início do tratamento. Os roedores tratados com dipropionato de imidocarb apresentaram recidiva da infecção após 30 dias. Na histopatologia não se observou alteração renal e hepática relacionada à doença ou aos medicamentos testados. Com base nos resultados, foi concluído que o aceturato de diminazeno, quando administrado por cinco dias consecutivos, é efetivo no tratamento da tripanossomose em ratos.The aim of this study was to evaluate the efficacy of Diminazene aceturate and imidocarb dipropionate in the control of Trypanosoma evansi infection in rats (Rattus norvegicus) experimentally infected. Fifty-four male rats were inoculated through intraperitoneal route with 104 T. evansi trypomastigotes. The rats were evaluated daily by periferic blood smears examination and treated when eight flagellated parasites were observed in 1000x microscopic field. Two therapeutics protocols were used. The first one included Groups A, B, C, D in which the rats were submitted to a single dose of the testing drugs administered by intramuscular route at the day 0 and again when T. evansi was observed in the blood smears. The rats of the second protocol (Groups E, F, G, H) were submitted to the same treatment by five consecutive days. Four rats (Group I) were used as control and were not submitted to any treatment. Tested drugs did not show any curative effect when used in the first protocol, since parasitaemia was evident few days after treatment. The use of Diminazene aceturate in the second protocol resulted in elimination of the trypomastigotes from circulation. In this case the rats were euthanized at the day 90. The infection recurred 30 days after the administration of imidocarb dipropionate. Histologically, no lesions were found in the liver or kidney. Diminazene aceturate is effective in treating trypanosomosis in rats when used five days consecutively
Josue De Moraes - One of the best experts on this subject based on the ideXlab platform.
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therapeutic effect of Diminazene aceturate on parasitic blood fluke schistosoma mansoni infection
Antimicrobial Agents and Chemotherapy, 2020Co-Authors: Mariana Brito, George Laylson Da Silva Oliveira, Ana C Mengarda, Maria E Cirino, Tais C Silva, Rosimeire Nunes De Oliveira, Silmara Marques Allegretti, Josue De MoraesAbstract:Praziquantel is currently the only drug available to treat schistosomiasis, a disease of enormous public health significance caused by a blood fluke of the genus Schistosoma Diminazene, a drug approved by the FDA, has been successfully used to treat diseases caused by blood protozoan parasites. In this study, we evaluated the antiparasitic properties of Diminazene against Schistosoma mansoniex vivo and in mice harboring either chronic or early S. mansoni infections. In vitro, we monitored phenotypic and tegumental changes as well as the effects of the drug on pairing and egg production. In mice infected with either adult (chronic infection) or immature (early infection) worms, Diminazene was administered intraperitoneally (10 to 100 mg/kg of body weight) or by oral gavage (100 to 400 mg/kg), and we studied the influence of the drug on worm burden and egg production. Liver and spleen pathologies and serum aminotransferase levels were also analyzed. In vitro, 50% effective concentrations (EC50) and EC90 revealed that Diminazene is able to kill both immature and adult parasites, and its effect was time and concentration dependent. In addition, confocal laser scanning microscopy showed morphological alterations in the teguments of schistosomes. In an animal model, the influence of the drug on worm burden, egg production, hepatomegaly, and splenomegaly depended on the dosing regimen applied and the route of administration. Diminazene also caused a significant reduction in aminotransferase levels. Comparatively, Diminazene treatment was more effective in chronic infection than in early infection. In tandem, our study revealed that Diminazene possesses anthelmintic properties and inhibits liver injury caused by Schistosoma eggs.
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therapeutic effect of Diminazene aceturate on parasitic blood fluke schistosoma mansoni infection
Antimicrobial Agents and Chemotherapy, 2020Co-Authors: Mariana Brito, George Laylson Da Silva Oliveira, Ana C Mengarda, Maria E Cirino, Tais C Silva, Rosimeire Nunes De Oliveira, Silmara Marques Allegretti, Josue De MoraesAbstract:Praziquantel is currently the only drug available to treat schistosomiasis, a disease of enormous public health significance caused by a blood fluke of the genus Schistosoma Diminazene, a drug approved by the FDA, has been successfully used to treat diseases caused by blood protozoan parasites. In this study, we evaluated the antiparasitic properties of Diminazene against S. mansoni ex vivo and in mice harboring either chronic or early S. mansoni infection. In vitro, we monitored phenotypic and tegumental changes as well as the effects of the drug on pairing and egg production. In a mouse infected with either adult (chronic infection) or immature (early infection) worms, Diminazene was administered intraperitoneally (10-100 mg/kg) or by oral gavage (100-400 mg/kg), and we studied the influence of drug on worm burden and egg production. Liver and spleen pathologies and serum aminotransferase levels were also analyzed. In vitro, EC50 and EC90 values revealed that Diminazene is able to kill both immature and adult parasites, and its effect was time- and concentration-dependent. In addition, confocal laser scanning microscopy showed morphological alterations in the tegument of schistosomes. In an animal model, the influence of the drug on worm burden, egg production, hepatomegaly, and splenomegaly depended on the dosing regimen applied and route of administration. Diminazene also caused a significant reduction in aminotransferase levels. Comparatively, Diminazene treatment was more effective in chronic infection than early infection. In tandem, our study revealed that Diminazene possesses anthelmintic properties and it improves liver injury caused by Schistosoma eggs.