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Akira Ishih - One of the best experts on this subject based on the ideXlab platform.
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Worm Burden and mucosal mast cell response in da and f344 n rat strains infected with hymenolepis diminuta
International Journal for Parasitology, 1994Co-Authors: Akira IshihAbstract:Abstract Worm Burden and mucosal mast cell response in DA and F344/N rat strains infected with Hymenolepis diminuta. International Journal for Parasitology24: 295–298. After oral administration of 1 or 5 cysticercoids of Hymenolepis diminuta, 5-week-old DA male rats showed significant mastocytosis. In F344/N rats, however, neither mastocytosis nor Worm loss occurred during a 6 week infection. With regard to mucosal mast cell response to infection with H. diminuta, DA rats can be looked on as high responders and F344/N rats as low responders.
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Worm Burden and mucosal mast cell response in DA and F344/n rat strains infected with hymenolepis diminuta
International journal for parasitology, 1994Co-Authors: Akira IshihAbstract:Abstract Worm Burden and mucosal mast cell response in DA and F344/N rat strains infected with Hymenolepis diminuta. International Journal for Parasitology24: 295–298. After oral administration of 1 or 5 cysticercoids of Hymenolepis diminuta, 5-week-old DA male rats showed significant mastocytosis. In F344/N rats, however, neither mastocytosis nor Worm loss occurred during a 6 week infection. With regard to mucosal mast cell response to infection with H. diminuta, DA rats can be looked on as high responders and F344/N rats as low responders.
Shuhua Xiao - One of the best experts on this subject based on the ideXlab platform.
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efficacy of tribendimidine and albendazole in treating mice infected with trichinella spiralis
Chinese Journal of Parasitology and Parasitic Diseases, 2010Co-Authors: Jian Xue, Yong-nian Zhang, Shuhua Xiao, Hui-qin QiangAbstract:OBJECTIVE To observe the efficacy of tribendimidine and albendazole against Trichinella spiralis in mice. METHODS A total of 85 Kunming strain mice, infected orally with 100 T. spiralis larvae, was divided into 3 groups: group A (adult stage, 7 d after infection), group B (migrating larva stage, 15 d after infection), and group C (encapsulated larva stage, 35 d after infection). Group A (35 mice) was equally divided into 7 sub-groups, tribendimidine and albendazole were each orally administered to 3 sub-groups both with doses of 6.25, 12.5, and 25 mg/kg respectively, the untreated sub-group served as control. Groups B and C (25 mice each) were both divided equally into 5 sub-groups. Mice in 2 sub-groups were treated respectively with the 2 drugs in a dose of 100 or 200 mg/kg, the untreated sub-group served as control. Mice in group A were sacrificed 2 d post-treatment and adult Worms recovered from the small intestine were counted. Those in groups B and C were sacrificed 15 d post-treatment and intact diaphragm was then removed from each mouse. The muscle of diaphragm was digested by digestive solution and the larvae were counted by stereomicroscope. Mean Worm Burden and mean Worm reduction of each treated group were calculated and statistically compared with the control. RESULTS The mean Worm Burden in sub-groups of group A treated with tribendimidine was significantly lower than that of the control (P<0.01) with a mean Worm reduction of 63.3%, 86.2%, and 98.5%, respectively. In the same batch of mice treated with albendazole at a single dose of 6.25 and 12.5 mg/kg resulted in similar mean Worm Burden compared to the control (P<0.05). While in the sub-group received albendazole at a higher dose of 25 mg/kg, the mean Worm Burden was significantly lower than that of the control (P<0.05), with a mean Worm reduction of 41.2%. The mean Worm Burden in group B was significantly lower than that of the control (P<.01). The mean Worm reduction in the 2 sub-groups treated with tribendimidine or albendazole was 64.4% and 89.6%, or 56.7% and 78.4%, respectively. In group C, significantly lower mean Worm Burden was only found in the subgroup treated with albendazole at a higher dose of 200 mg/kg than the control (P<0.01) with a mean Worm reduction of 71.8%. No effect was seen in the other 3 groups. CONCLUSION Tribendimidine exhibits potential effect against adult and migrating larva stage of T. spiralis in mice, but lacks effect against encapsulated larva stage of the parasite. Albendazole administered at a larger or multiple doses to mice endorses effect against its adult, migrating larva and encapsulated larva stages.
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therapeutic effect of tribendimidine artesunate and praziquantel administered to hamsters infected with clonorchis sinensis
Chinese Journal of Parasitology and Parasitic Diseases, 2009Co-Authors: Jian Xue, Yong-nian Zhang, Hui-qin Qiang, Shuhua XiaoAbstract:OBJECTIVE: To observe the effect of tribendimidine, artesunate and praziquantel in treatment of hamsters (Mesocricetus auratus) infected with Clonorchis sinensis. METHODS: A total of 93 hamsters, each infected with 30 C. sinensis metacercariae, were treated intragastrically with above-mentioned drugs at a single dose. (1) In order to observe the effect of the drugs against juvenile C. sinensis, 20 out of 31 infected hamsters were randomly divided into 4 groups (5 hamsters per group) 14 d post-infection: artesunate 300 mg/kg, tribendimidine 100 mg/kg or 200 mg/kg, and praziquantel 200 mg/kg. Other 6 hamsters were divided equally into 2 groups 24 d post-infection and treated with tribendimidine 200 mg/kg and artesunate 300 mg/kg, respectively. The remained 5 untreated hamsters served as control. (2) Twenty-two hamsters were randomly divided into 5 groups (4-5 hamsters per group) 28 d post-infection and treated with tribendimidine 25 mg/kg or 50 mg/kg, artesunate 25 mg/kg and praziquantel 50 mg/kg, respectively. Other untreated hamsters served as control. (3) Forty hamsters 28 d after infection were randomly divided into 8 groups (4-6 hamsters per group) and treated with tribendimidine 50 mg/kg, 100 mg/kg or 200 mg/kg, artesunate 100 mg/kg or 200 mg/kg, praziquantel 100mg/kg or 200mg/kg, respectively. The remained hamsters served as control. All hamsters were sacrificed 14 d post-treatment and Worms were recovered from the bile duct and liver tissue. The mean Worm Burden and its reduction were calculated. The differences of mean Worm Burden between each treated group and the corresponding control were analyzed statistically. RESULTS: In hamsters infected with 14-d-old C. sinensis and treated orally with tribendimidine at a single dose of 100 or 200 mg/kg, the mean Worm Burdens were significantly lower than that of the control (P<0.01) with a Worm reduction of 90.6% and 85.9% respectively. The mean Worm Burden obtained from the infected hamsters treated with praziquantel at a single dose of 200 mg/kg was also significantly lower than that of the control (P<0.05) with a Worm reduction of 71.9%. However, the difference of mean Worm Burden between artesunate and control groups was not statistically significant. The juvenile parasites developed into adult Worms 24 d after infection. By administering tribendimidine 200 mg/kg to the adult C. sinensis-infected hamsters, the mean Worm Burden was significantly lower than that of the control (P<0.01) with a Worm reduction of 89.8%. Whilst the administration of artesunate at a higher dose of 300 mg/kg, all hamsters were cured. Further tests indicated that tribendimidine in a lower dose of 25 mg/kg to the hamsters 28 d after infection resulted in a significantly lower mean Worm Burden compared to the control (P<0.05) with a Worm reduction of 71.8%. With an increased dose of tribendimidine 100 mg/kg, all hamsters were cured. The Worm reduction was only 20.0% and 56.4% when 25 mg/kg and 100 mg/kg of artesunate were administered. With 200 mg/kg artesunate, the Worm reduction reached as high as 98.5% and the mean Worm Burden was significantly lower than that of the control (P<0.01). Furthermore, administration of praziquantel at a dose of 100 mg/kg or 200 mg/kg at 28 d post-infection resulted in a significantly lower mean Worm Burden than that of the control (P<0.05) with a Worm reduction of 78.9% and 83.5% respectively. CONCLUSION: In hamster model, tribendimidine and praziquantel exhibit promising effect against both juvenile and adult C. sinensis, while artesunate is only efficacious against adult Worms.
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artemether artesunate praziquantel and tribendimidine administered singly at different dosages against clonorchis sinensis a comparative in vivo study
Acta Tropica, 2008Co-Authors: Shuhua Xiao, Marcel Tanner, Jennifer Keiser, Jürg Utzinger, Xue Jian, Zhang Yongnian, Qiang HuiqiangAbstract:We comparatively assessed the in vivo efficacy of artemether, artesunate, praziquantel and tribendimidine against different stages of Clonorchis sinensis. Rats were infected with 40-50 C. sinensis metacercariae, and drugs were administered singly by the oral route at different dosages. Rats were dissected 2-4 weeks post-treatment and C. sinensis trematodes were removed from the liver and bile ducts and counted. We used a negative binomial regression model to test the effect of drug and dosage in terms of Worm Burden reduction. Single 150 mg/kg oral doses of artesunate, artemether, tribendimidine and praziquantel, administered to rats infected with adult C. sinensis, resulted in mean Worm Burden reductions of 100, 100, 89.5 and 80.7%, respectively (all P<0.001). Halving the dose to 75 mg/kg still resulted in highly significant Worm Burden reductions for artesunate, artemether and tribendimidine (71.4-100%), but not for praziquantel (20.7%). In the juvenile infection model, a single 150 mg/kg oral dose of tribendimidine and praziquantel resulted in mean Worm Burden reductions of 99.1 and 90.0%, respectively, whereas considerably lower reductions were observed for artemether (59.2%) and artesunate (57.6%) when used at the same single dose. The in vivo results presented here with the artemisinins and tribendimidine provide further data for clinical investigations to assess the safety and efficacy of these drugs in clonorchiasis patients.
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Artemether, artesunate, praziquantel and tribendimidine administered singly at different dosages against Clonorchis sinensis : a comparative in vivo study
Acta tropica, 2008Co-Authors: Shuhua Xiao, Marcel Tanner, Jennifer Keiser, Jürg Utzinger, Xue Jian, Zhang Yong-nian, Qiang Hui-qiangAbstract:We comparatively assessed the in vivo efficacy of artemether, artesunate, praziquantel and tribendimidine against different stages of Clonorchis sinensis. Rats were infected with 40-50 C. sinensis metacercariae, and drugs were administered singly by the oral route at different dosages. Rats were dissected 2-4 weeks post-treatment and C. sinensis trematodes were removed from the liver and bile ducts and counted. We used a negative binomial regression model to test the effect of drug and dosage in terms of Worm Burden reduction. Single 150 mg/kg oral doses of artesunate, artemether, tribendimidine and praziquantel, administered to rats infected with adult C. sinensis, resulted in mean Worm Burden reductions of 100, 100, 89.5 and 80.7%, respectively (all P
Jerzy M Behnke - One of the best experts on this subject based on the ideXlab platform.
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density dependent regulation of the growth of the hookWorms necator americanus and ancylostoma ceylanicum
Parasitology, 1994Co-Authors: S M B Norozianamiri, Jerzy M BehnkeAbstract:Laboratory bred DSN hamsters were exposed to varying doses of infective larvae of Ancylostoma ceylanicum (orally) or Necator americanus (percutaneously) and were autopsied at times which corresponded to a period immediately before cessation of growth of Worms or soon afterwards. A total of 829 (404 male and 425 female) A. ceylanicum and 1582 (781 male and 801 female) N. americanus were measured. At Worm Burdens of fewer than 100, the length of A. ceylanicum appeared to increase with infection intensity and no evidence was found that growth was retarded under crowded conditions. In an experiment comparing directly low (mean Worm Burden=22) and heavy infections (mean Worm Burden =180) significant negative associations between both weight and width, and Worm Burden were detected, but length again increased with form Burden
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Density-dependent regulation of the growth of the hookWorms Necator americanus and Ancylostoma ceylanicum.
Parasitology, 1994Co-Authors: S. M. B. Norozian-amiri, Jerzy M BehnkeAbstract:Laboratory bred DSN hamsters were exposed to varying doses of infective larvae of Ancylostoma ceylanicum (orally) or Necator americanus (percutaneously) and were autopsied at times which corresponded to a period immediately before cessation of growth of Worms or soon afterwards. A total of 829 (404 male and 425 female) A. ceylanicum and 1582 (781 male and 801 female) N. americanus were measured. At Worm Burdens of fewer than 100, the length of A. ceylanicum appeared to increase with infection intensity and no evidence was found that growth was retarded under crowded conditions. In an experiment comparing directly low (mean Worm Burden = 22) and heavy infections (mean Worm Burden = 180) significant negative associations between both weight and width, and Worm Burden were detected, but length again increased with Worm Burden. In contrast, 5 experiments with N. americanus indicated negative relationships between measures of Worm size (length, width, wet and dry weight) and Worm Burden. It was concluded that N. americanus is subject to regulation by density-dependent processes within the host while A. ceylanicum is not sensitive to the same degree.
Pedro L S Pinto - One of the best experts on this subject based on the ideXlab platform.
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promethazine exhibits antiparasitic properties in vitro and reduces Worm Burden egg production hepatomegaly and splenomegaly in a schistosomiasis animal model
Antimicrobial Agents and Chemotherapy, 2019Co-Authors: Daniel B Roquini, Ramon M Cogo, Ana C Mengarda, Susana F Mazloum, Cristiane S Morais, Rogerio P Xavier, M C Salvadori, F S Teixeira, Luiz E Ferreira, Pedro L S PintoAbstract:: The treatment and control of schistosomiasis, a neglected disease that affects more than 200 million people worldwide, rely on the use of a single drug, praziquantel. A vaccine has yet to be developed and since new drug design and development is a lengthy and costly process, drug repurposing is a promising strategy. In this study, the efficacy of promethazine, a first-generation antihistamine, was evaluated against Schistosoma mansoniex vivo and in a murine model of schistosomiasis. In vitro assays demonstrated that promethazine affected parasite motility, viability, and it induced severe tegumental damage in schistosomes. The LC50 of the drug was 5.84 μM. Similar to promethazine, schistosomes incubated with atropine, a classical anticholinergic drug, displayed reduced motor activity. In an animal model, promethazine treatment was introduced at an oral dose of 100 mg/kg for five successive days at different intervals from the time of infection, for the evaluation of the stage-specific susceptibility (pre-patent and patent infections). Various parasitological criteria indicated the in vivo antischistosomal effects of promethazine: there were significant reductions in Worm Burden, egg production, and hepato- and splenomegaly. The highest Worm Burden reduction was achieved with promethazine in patent infections (> 90%). Taken together, considering the importance of the repositioning of drugs in infectious diseases, especially those related to poverty, our data revealed the possibility of promethazine repositioning as an antischistosomal agent.
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Promethazine exhibits antiparasitic properties in vitro and reduces Worm Burden, egg production, hepato-, and splenomegaly in a schistosomiasis animal model.
Antimicrobial agents and chemotherapy, 2019Co-Authors: Daniel B Roquini, Ramon M Cogo, Ana C Mengarda, Susana F Mazloum, Cristiane S Morais, Luiz E Ferreira, R. Xavier, Maria Cecília Barbosa Da Silveira Salvadori, Fernanda De Sá Teixeira, Pedro L S PintoAbstract:The treatment and control of schistosomiasis, a neglected disease that affects more than 200 million people worldwide, rely on the use of a single drug, praziquantel. A vaccine has yet to be developed and since new drug design and development is a lengthy and costly process, drug repurposing is a promising strategy. In this study, the efficacy of promethazine, a first-generation antihistamine, was evaluated against Schistosoma mansoni ex vivo and in a murine model of schistosomiasis. In vitro assays demonstrated that promethazine affected parasite motility, viability, and it induced severe tegumental damage in schistosomes. The LC50 of the drug was 5.84 μM. Similar to promethazine, schistosomes incubated with atropine, a classical anticholinergic drug, displayed reduced motor activity. In an animal model, promethazine treatment was introduced at an oral dose of 100 mg/kg for five successive days at different intervals from the time of infection, for the evaluation of the stage-specific susceptibility (pre-patent and patent infections). Various parasitological criteria indicated the in vivo antischistosomal effects of promethazine: there were significant reductions in Worm Burden, egg production, and hepato- and splenomegaly. The highest Worm Burden reduction was achieved with promethazine in patent infections (> 90%). Taken together, considering the importance of the repositioning of drugs in infectious diseases, especially those related to poverty, our data revealed the possibility of promethazine repositioning as an antischistosomal agent.
Qiang Huiqiang - One of the best experts on this subject based on the ideXlab platform.
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artemether artesunate praziquantel and tribendimidine administered singly at different dosages against clonorchis sinensis a comparative in vivo study
Acta Tropica, 2008Co-Authors: Shuhua Xiao, Marcel Tanner, Jennifer Keiser, Jürg Utzinger, Xue Jian, Zhang Yongnian, Qiang HuiqiangAbstract:We comparatively assessed the in vivo efficacy of artemether, artesunate, praziquantel and tribendimidine against different stages of Clonorchis sinensis. Rats were infected with 40-50 C. sinensis metacercariae, and drugs were administered singly by the oral route at different dosages. Rats were dissected 2-4 weeks post-treatment and C. sinensis trematodes were removed from the liver and bile ducts and counted. We used a negative binomial regression model to test the effect of drug and dosage in terms of Worm Burden reduction. Single 150 mg/kg oral doses of artesunate, artemether, tribendimidine and praziquantel, administered to rats infected with adult C. sinensis, resulted in mean Worm Burden reductions of 100, 100, 89.5 and 80.7%, respectively (all P<0.001). Halving the dose to 75 mg/kg still resulted in highly significant Worm Burden reductions for artesunate, artemether and tribendimidine (71.4-100%), but not for praziquantel (20.7%). In the juvenile infection model, a single 150 mg/kg oral dose of tribendimidine and praziquantel resulted in mean Worm Burden reductions of 99.1 and 90.0%, respectively, whereas considerably lower reductions were observed for artemether (59.2%) and artesunate (57.6%) when used at the same single dose. The in vivo results presented here with the artemisinins and tribendimidine provide further data for clinical investigations to assess the safety and efficacy of these drugs in clonorchiasis patients.
