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Bruce D. Hammock - One of the best experts on this subject based on the ideXlab platform.

  • An immunoassay for the detection of triclosan-O-glucuronide, a primary human urinary metabolite of triclosan
    Analytical and Bioanalytical Chemistry, 2015
    Co-Authors: Anupama Ranganathan, Bruce D. Hammock
    Abstract:

    Triclosan- O -glucuronide (TCSG) is one of the primary urinary metabolites of the antibacterial compound triclosan or TCS that is found in many personal care products and consumer goods. We have developed a competitive, indirect heterologous ELISA for the detection of the target TCSG in urine. Such an ELISA for TCSG could be developed as a useful tool to measure this important biomarker of human exposure to TCS. Immunogens were prepared by conjugating TCSG to thyroglobulin, via heterobifunctional cross-linkers AEDP or 3-[(2-aminoethyl)dithio] propionic acid•hydrochloride and TFCS or N -[ε-trifluoroacetylcaproyloxy]succinimide ester. The coating antigen was prepared by the direct conjugation of TCSG to bovine serum albumin. Antibodies raised in rabbits 2619, 2621 (immunogen TCSG-AEDP-Thy), and 2623 (immunogen TCSG-TFCS-Thy), and the coating antigen were screened and characterized to determine their optimal concentrations. The optimized ELISA, developed with antibody 2621, gave an IC_50 value of 2.85 ng/mL, with the linear range (IC_20–IC_80) determined to be 2.6–24.8 ng/mL. Selectivity of the assay was assessed by measuring cross-reactivity of antibody 2621 to related congeners such as the aglycone TCS, triclosan- O -sulfate, triclocarban, a polybrominated Diphenyl Ether Derivative, and 3-phenoxybenzyl alcohol glucuronide. There was virtually no recognition by antibody 2621 to any of these cross-reactants. Graphical Abstract Urinary biomarker analysis of triclosan glucuronide

  • An immunoassay for the detection of triclosan- O -glucuronide, a primary human urinary metabolite of triclosan
    Analytical and Bioanalytical Chemistry, 2015
    Co-Authors: Anupama Ranganathan, Shirley J. Gee, Bruce D. Hammock
    Abstract:

    Triclosan-O-glucuronide (TCSG) is one of the primary urinary metabolites of the antibacterial compound triclosan or TCS that is found in many personal care products and consumer goods. We have developed a competitive, indirect heterologous ELISA for the detection of the target TCSG in urine. Such an ELISA for TCSG could be developed as a useful tool to measure this important biomarker of human exposure to TCS. Immunogens were prepared by conjugating TCSG to thyroglobulin, via heterobifunctional cross-linkers AEDP or 3-[(2-aminoethyl)dithio] propionic acid•hydrochloride and TFCS or N-[e-trifluoroacetylcaproyloxy]succinimide ester. The coating antigen was prepared by the direct conjugation of TCSG to bovine serum albumin. Antibodies raised in rabbits 2619, 2621 (immunogen TCSG-AEDP-Thy), and 2623 (immunogen TCSG-TFCS-Thy), and the coating antigen were screened and characterized to determine their optimal concentrations. The optimized ELISA, developed with antibody 2621, gave an IC50 value of 2.85 ng/mL, with the linear range (IC20–IC80) determined to be 2.6–24.8 ng/mL. Selectivity of the assay was assessed by measuring cross-reactivity of antibody 2621 to related congeners such as the aglycone TCS, triclosan-O-sulfate, triclocarban, a polybrominated Diphenyl Ether Derivative, and 3-phenoxybenzyl alcohol glucuronide. There was virtually no recognition by antibody 2621 to any of these cross-reactants.

Anupama Ranganathan - One of the best experts on this subject based on the ideXlab platform.

  • An immunoassay for the detection of triclosan-O-glucuronide, a primary human urinary metabolite of triclosan
    Analytical and Bioanalytical Chemistry, 2015
    Co-Authors: Anupama Ranganathan, Bruce D. Hammock
    Abstract:

    Triclosan- O -glucuronide (TCSG) is one of the primary urinary metabolites of the antibacterial compound triclosan or TCS that is found in many personal care products and consumer goods. We have developed a competitive, indirect heterologous ELISA for the detection of the target TCSG in urine. Such an ELISA for TCSG could be developed as a useful tool to measure this important biomarker of human exposure to TCS. Immunogens were prepared by conjugating TCSG to thyroglobulin, via heterobifunctional cross-linkers AEDP or 3-[(2-aminoethyl)dithio] propionic acid•hydrochloride and TFCS or N -[ε-trifluoroacetylcaproyloxy]succinimide ester. The coating antigen was prepared by the direct conjugation of TCSG to bovine serum albumin. Antibodies raised in rabbits 2619, 2621 (immunogen TCSG-AEDP-Thy), and 2623 (immunogen TCSG-TFCS-Thy), and the coating antigen were screened and characterized to determine their optimal concentrations. The optimized ELISA, developed with antibody 2621, gave an IC_50 value of 2.85 ng/mL, with the linear range (IC_20–IC_80) determined to be 2.6–24.8 ng/mL. Selectivity of the assay was assessed by measuring cross-reactivity of antibody 2621 to related congeners such as the aglycone TCS, triclosan- O -sulfate, triclocarban, a polybrominated Diphenyl Ether Derivative, and 3-phenoxybenzyl alcohol glucuronide. There was virtually no recognition by antibody 2621 to any of these cross-reactants. Graphical Abstract Urinary biomarker analysis of triclosan glucuronide

  • An immunoassay for the detection of triclosan- O -glucuronide, a primary human urinary metabolite of triclosan
    Analytical and Bioanalytical Chemistry, 2015
    Co-Authors: Anupama Ranganathan, Shirley J. Gee, Bruce D. Hammock
    Abstract:

    Triclosan-O-glucuronide (TCSG) is one of the primary urinary metabolites of the antibacterial compound triclosan or TCS that is found in many personal care products and consumer goods. We have developed a competitive, indirect heterologous ELISA for the detection of the target TCSG in urine. Such an ELISA for TCSG could be developed as a useful tool to measure this important biomarker of human exposure to TCS. Immunogens were prepared by conjugating TCSG to thyroglobulin, via heterobifunctional cross-linkers AEDP or 3-[(2-aminoethyl)dithio] propionic acid•hydrochloride and TFCS or N-[e-trifluoroacetylcaproyloxy]succinimide ester. The coating antigen was prepared by the direct conjugation of TCSG to bovine serum albumin. Antibodies raised in rabbits 2619, 2621 (immunogen TCSG-AEDP-Thy), and 2623 (immunogen TCSG-TFCS-Thy), and the coating antigen were screened and characterized to determine their optimal concentrations. The optimized ELISA, developed with antibody 2621, gave an IC50 value of 2.85 ng/mL, with the linear range (IC20–IC80) determined to be 2.6–24.8 ng/mL. Selectivity of the assay was assessed by measuring cross-reactivity of antibody 2621 to related congeners such as the aglycone TCS, triclosan-O-sulfate, triclocarban, a polybrominated Diphenyl Ether Derivative, and 3-phenoxybenzyl alcohol glucuronide. There was virtually no recognition by antibody 2621 to any of these cross-reactants.

Chang-yun Wang - One of the best experts on this subject based on the ideXlab platform.

  • Antifouling Phenyl Ethers and Other Compounds from the Invertebrates and Their Symbiotic Fungi Collected from the South China Sea
    AMB Express, 2016
    Co-Authors: Chao-yi Wang, Min Chen, Chang-lun Shao, Kai-ling Wang, Pei-yuan Qian, Juan-juan Zheng, Min Liu, Chang-yun Wang
    Abstract:

    Marine organism-derived secondary metabolites are promising potential sources for discovering environmentally safe antifouling agents. In present study, 55 marine secondary metabolites and their synthesized Derivatives were tested and evaluated for their antifouling activities and security. These compounds include 44 natural products isolated from marine invertebrates and their symbiotic microorganisms collected from the South China Sea and 11 structural modified products derived from the isolated compounds. The natural secondary metabolites, covering phenyl Ether Derivatives, terpenoids, 9, 11-secosteroids, anthraquinones, alkaloids, nucleoside Derivatives and peptides, were isolated from two corals, one sponge and five symbiotic fungi. All of the isolated and synthesized compounds were tested for their antifouling activities against the cyprids of barnacle Balanus (Amphibalanus) amphitrite Darwin. Noticeably, five phenyl Ether Derivatives (9, 11, 13–15) exhibited potent anti-larval settlement activity with the EC50 values lower than 3.05 μM and the LC50/EC50 ratios higher than 15. The study of structure–activity relationship (SAR) revealed that the introduction of acetoxy groups and bromine atoms to phenyl Ether Derivatives could significantly improve their antifouling activities. This is the first report on the SAR of phenyl Ether Derivatives on antifouling activity against barnacle B. amphitrite. The polybrominated Diphenyl Ether Derivative, 2, 4, 6, 2′, 4′, 6′-hexabromo-diorcinol (13), which displayed excellent antifouling activity, was considered as a promising candidate of environmentally friendly antifouling agents.

  • Azaphilone and Diphenyl Ether Derivatives from a Gorgonian-Derived Strain of the Fungus Penicillium pinophilum
    Journal of Natural Products, 2015
    Co-Authors: Dong-lin Zhao, Chang-lun Shao, Qiang Zhang, Kai-ling Wang, Fei-fei Guan, Ting Shi, Chang-yun Wang
    Abstract:

    Three new azaphilone Derivatives, pinophilins D–F (1–3), and one new Diphenyl Ether Derivative, hydroxypenicillide (10), togEther with nine known compounds (4–9, 11–13), were isolated from the gorgonian-derived fungus Penicillium pinophilum XS-20090E18. Their structures including absolute configurations were determined by spectroscopic data, chemical conversions, the ECD exciton chirality method, and ECD calculations. Compounds 10–13 exhibited inhibitory activity against the larval settlement of the barnacle Balanus amphitrite at nontoxic concentrations. Compounds 10 and 11 showed cytotoxicity against Hep-2, RD, and HeLa cell lines.

Min Chen - One of the best experts on this subject based on the ideXlab platform.

  • A new Diphenyl Ether Derivative from Mirabilis himalaica
    Natural Product Research, 2016
    Co-Authors: Si-yu Zhou, Guo-wei Wang, Yan-lin Zou, Li-qing Deng, Mingxu Liu, Zhihua Liao, Xiaozhong Lan, Min Chen
    Abstract:

    One new compound, himalain A (1), togEther with 12 known compounds were isolated from Mirabilis himalaica. Their structures were determined by spectroscopic methods, including 2D NMR techniques, and the absolute configuration of the 1,2-diol moiety in 1 was defined through Riguera's method. All isolated compounds were tested for their cytotoxic and antibacterial activities.

  • Antifouling Phenyl Ethers and Other Compounds from the Invertebrates and Their Symbiotic Fungi Collected from the South China Sea
    AMB Express, 2016
    Co-Authors: Chao-yi Wang, Min Chen, Chang-lun Shao, Kai-ling Wang, Pei-yuan Qian, Juan-juan Zheng, Min Liu, Chang-yun Wang
    Abstract:

    Marine organism-derived secondary metabolites are promising potential sources for discovering environmentally safe antifouling agents. In present study, 55 marine secondary metabolites and their synthesized Derivatives were tested and evaluated for their antifouling activities and security. These compounds include 44 natural products isolated from marine invertebrates and their symbiotic microorganisms collected from the South China Sea and 11 structural modified products derived from the isolated compounds. The natural secondary metabolites, covering phenyl Ether Derivatives, terpenoids, 9, 11-secosteroids, anthraquinones, alkaloids, nucleoside Derivatives and peptides, were isolated from two corals, one sponge and five symbiotic fungi. All of the isolated and synthesized compounds were tested for their antifouling activities against the cyprids of barnacle Balanus (Amphibalanus) amphitrite Darwin. Noticeably, five phenyl Ether Derivatives (9, 11, 13–15) exhibited potent anti-larval settlement activity with the EC50 values lower than 3.05 μM and the LC50/EC50 ratios higher than 15. The study of structure–activity relationship (SAR) revealed that the introduction of acetoxy groups and bromine atoms to phenyl Ether Derivatives could significantly improve their antifouling activities. This is the first report on the SAR of phenyl Ether Derivatives on antifouling activity against barnacle B. amphitrite. The polybrominated Diphenyl Ether Derivative, 2, 4, 6, 2′, 4′, 6′-hexabromo-diorcinol (13), which displayed excellent antifouling activity, was considered as a promising candidate of environmentally friendly antifouling agents.

Kai-ling Wang - One of the best experts on this subject based on the ideXlab platform.

  • Antifouling Phenyl Ethers and Other Compounds from the Invertebrates and Their Symbiotic Fungi Collected from the South China Sea
    AMB Express, 2016
    Co-Authors: Chao-yi Wang, Min Chen, Chang-lun Shao, Kai-ling Wang, Pei-yuan Qian, Juan-juan Zheng, Min Liu, Chang-yun Wang
    Abstract:

    Marine organism-derived secondary metabolites are promising potential sources for discovering environmentally safe antifouling agents. In present study, 55 marine secondary metabolites and their synthesized Derivatives were tested and evaluated for their antifouling activities and security. These compounds include 44 natural products isolated from marine invertebrates and their symbiotic microorganisms collected from the South China Sea and 11 structural modified products derived from the isolated compounds. The natural secondary metabolites, covering phenyl Ether Derivatives, terpenoids, 9, 11-secosteroids, anthraquinones, alkaloids, nucleoside Derivatives and peptides, were isolated from two corals, one sponge and five symbiotic fungi. All of the isolated and synthesized compounds were tested for their antifouling activities against the cyprids of barnacle Balanus (Amphibalanus) amphitrite Darwin. Noticeably, five phenyl Ether Derivatives (9, 11, 13–15) exhibited potent anti-larval settlement activity with the EC50 values lower than 3.05 μM and the LC50/EC50 ratios higher than 15. The study of structure–activity relationship (SAR) revealed that the introduction of acetoxy groups and bromine atoms to phenyl Ether Derivatives could significantly improve their antifouling activities. This is the first report on the SAR of phenyl Ether Derivatives on antifouling activity against barnacle B. amphitrite. The polybrominated Diphenyl Ether Derivative, 2, 4, 6, 2′, 4′, 6′-hexabromo-diorcinol (13), which displayed excellent antifouling activity, was considered as a promising candidate of environmentally friendly antifouling agents.

  • Azaphilone and Diphenyl Ether Derivatives from a Gorgonian-Derived Strain of the Fungus Penicillium pinophilum
    Journal of Natural Products, 2015
    Co-Authors: Dong-lin Zhao, Chang-lun Shao, Qiang Zhang, Kai-ling Wang, Fei-fei Guan, Ting Shi, Chang-yun Wang
    Abstract:

    Three new azaphilone Derivatives, pinophilins D–F (1–3), and one new Diphenyl Ether Derivative, hydroxypenicillide (10), togEther with nine known compounds (4–9, 11–13), were isolated from the gorgonian-derived fungus Penicillium pinophilum XS-20090E18. Their structures including absolute configurations were determined by spectroscopic data, chemical conversions, the ECD exciton chirality method, and ECD calculations. Compounds 10–13 exhibited inhibitory activity against the larval settlement of the barnacle Balanus amphitrite at nontoxic concentrations. Compounds 10 and 11 showed cytotoxicity against Hep-2, RD, and HeLa cell lines.