The Experts below are selected from a list of 1929 Experts worldwide ranked by ideXlab platform

Guido Favia - One of the best experts on this subject based on the ideXlab platform.

  • unusual organization of the 5s ribosomal spacer in Dirofilaria repens absence of a canonical spliced leader 1 sequence
    Parasitology Research, 2000
    Co-Authors: Guido Favia, Gabriella Cancrini, C Bazzocchi, C Genchi, Claudio Bandi
    Abstract:

    Comparing the 5S ribosomal RNA spacer of Dirofilaria repens and D. immitis, we found that in D. repens PCR generates two amplification products. In both products, the putative spliced leader 1 sequences (SL1) show some nucleotide substitutions compared to the canonical SL1 sequence of nematodes. SL1 is a short sequence transferred to the 5′ end of most messenger RNAs through a trans-splicing reaction. All the other filariae studied thus far amplify a single fragment showing a canonical SL1 sequence. D. repens appears thus peculiar.

  • polymerase chain reaction identification of Dirofilaria repens and Dirofilaria immitis
    Parasitology, 1996
    Co-Authors: Guido Favia, A Lanfrancotti, Augusto Della Torre, Gabriella Cancrini, Mario Coluzzi
    Abstract:

    On the basis of known DNA sequences of Dirofilaria repens and D. immitis we designed specific primers for the amplification by Polymerase Chain Reaction (PCR) of the DNA from the two species. The PCR-based identification was found to be unambiguous and allowed specific diagnosis of microfilariae in blood samples, of developing larvae in the mosquito vector and of immature adults in bioptic material, overcoming the serious constraints of the morphological separation of these filarial parasites at the pre-adult stages. The technique was found to be very sensitive and applicable to samples stored either dry or in various preservation media, with the exception of formalin. The reliable identification of D. repens and D. immitis from bioptic material is expected to greatly enhance the chances of detecting human infections and to further clarify the role of the two parasites as pathogens of man. The possibility of routine identification of developing larvae in the vector will substantially improve the perspectives for epidemiological investigations, particularly in Southern European regions, such as Italy, where the two nematode species are largely sympatric.

Gabriella Cancrini - One of the best experts on this subject based on the ideXlab platform.

  • unusual organization of the 5s ribosomal spacer in Dirofilaria repens absence of a canonical spliced leader 1 sequence
    Parasitology Research, 2000
    Co-Authors: Guido Favia, Gabriella Cancrini, C Bazzocchi, C Genchi, Claudio Bandi
    Abstract:

    Comparing the 5S ribosomal RNA spacer of Dirofilaria repens and D. immitis, we found that in D. repens PCR generates two amplification products. In both products, the putative spliced leader 1 sequences (SL1) show some nucleotide substitutions compared to the canonical SL1 sequence of nematodes. SL1 is a short sequence transferred to the 5′ end of most messenger RNAs through a trans-splicing reaction. All the other filariae studied thus far amplify a single fragment showing a canonical SL1 sequence. D. repens appears thus peculiar.

  • polymerase chain reaction identification of Dirofilaria repens and Dirofilaria immitis
    Parasitology, 1996
    Co-Authors: Guido Favia, A Lanfrancotti, Augusto Della Torre, Gabriella Cancrini, Mario Coluzzi
    Abstract:

    On the basis of known DNA sequences of Dirofilaria repens and D. immitis we designed specific primers for the amplification by Polymerase Chain Reaction (PCR) of the DNA from the two species. The PCR-based identification was found to be unambiguous and allowed specific diagnosis of microfilariae in blood samples, of developing larvae in the mosquito vector and of immature adults in bioptic material, overcoming the serious constraints of the morphological separation of these filarial parasites at the pre-adult stages. The technique was found to be very sensitive and applicable to samples stored either dry or in various preservation media, with the exception of formalin. The reliable identification of D. repens and D. immitis from bioptic material is expected to greatly enhance the chances of detecting human infections and to further clarify the role of the two parasites as pathogens of man. The possibility of routine identification of developing larvae in the vector will substantially improve the perspectives for epidemiological investigations, particularly in Southern European regions, such as Italy, where the two nematode species are largely sympatric.

Mario Coluzzi - One of the best experts on this subject based on the ideXlab platform.

  • polymerase chain reaction identification of Dirofilaria repens and Dirofilaria immitis
    Parasitology, 1996
    Co-Authors: Guido Favia, A Lanfrancotti, Augusto Della Torre, Gabriella Cancrini, Mario Coluzzi
    Abstract:

    On the basis of known DNA sequences of Dirofilaria repens and D. immitis we designed specific primers for the amplification by Polymerase Chain Reaction (PCR) of the DNA from the two species. The PCR-based identification was found to be unambiguous and allowed specific diagnosis of microfilariae in blood samples, of developing larvae in the mosquito vector and of immature adults in bioptic material, overcoming the serious constraints of the morphological separation of these filarial parasites at the pre-adult stages. The technique was found to be very sensitive and applicable to samples stored either dry or in various preservation media, with the exception of formalin. The reliable identification of D. repens and D. immitis from bioptic material is expected to greatly enhance the chances of detecting human infections and to further clarify the role of the two parasites as pathogens of man. The possibility of routine identification of developing larvae in the vector will substantially improve the perspectives for epidemiological investigations, particularly in Southern European regions, such as Italy, where the two nematode species are largely sympatric.

Carla Maia - One of the best experts on this subject based on the ideXlab platform.

  • detection of Dirofilaria repens microfilariae in a dog from portugal
    Parasitology Research, 2016
    Co-Authors: Carla Maia, Susanne Lorentz, Luis Cardoso, Domenico Otranto, Torsten J Naucke
    Abstract:

    Dirofilaria repens causes subcutaneous infection in dogs and cats, and is the main agent of human dirofilariosis in Europe. Detection of D. repens is described in a dog from Portugal, a finding that simultaneously represents the first presumable case of autochthonous infection with the parasite in any animal host species in the country. A mixed D. repens/Dirofilaria immitis infection (20:1 ratio) was found, with an overall density of 1267 microfilariae per millilitre of blood. Morphological features, including morphometry, and acid phosphatase histochemical staining confirmed identity of the two filarioids. Distribution of D. repens in Portugal should be further assessed at the population level both in vertebrate hosts and in vectors. Due to the zoonotic potential of the parasite, preventive measures such as the administration of microfilaricides and insect repellents should be put into practice to protect animals and public health.

Matthias Schmuth - One of the best experts on this subject based on the ideXlab platform.