The Experts below are selected from a list of 318 Experts worldwide ranked by ideXlab platform
Shuichi Yamamoto - One of the best experts on this subject based on the ideXlab platform.
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Optimization of elution salt concentration in stepwise elution of protein chromatography using linear gradient elution data. Reducing residual protein A by cation-exchange chromatography in monoclonal antibody purification.
Journal of Chromatography A, 2006Co-Authors: Takashi Ishihara, Toshihiko Kadoya, Naomi Endo, Shuichi YamamotoAbstract:Our simple method for optimization of the elution salt concentration in stepwise elution was applied to the actual protein separation system, which involves several difficulties such as detection of the target. As a model separation system, reducing residual protein A by cation-exchange chromatography in human monoclonal antibody (hMab) purification was chosen. We carried out linear gradient elution experiments and obtained the data for the peak salt concentration of hMab and residual protein A, respectively. An enzyme-linked immunosorbent assay was applied to the measurement of the residual protein A. From these data, we calculated the Distribution Coefficient of the hMab and the residual protein A as a function of salt concentration. The optimal salt concentration of stepwise elution to reduce the residual protein A from the hMab was determined based on the relationship between the Distribution Coefficient and the salt concentration. Using the optimized condition, we successfully performed the separation, resulting in high recovery of hMab and the elimination of residual protein A.
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optimization of monoclonal antibody purification by ion exchange chromatography application of simple methods with linear gradient elution experimental data
Journal of Chromatography A, 2005Co-Authors: Takashi Ishihara, Shuichi YamamotoAbstract:Simple methods for the optimization of ion-exchange chromatography of proteins in our previous papers were applied to cation-exchange chromatography purification of monoclonal antibodies (Mab). We carried out linear gradient elution experiments, and obtained the data for the peak salt concentration and the peak width. From these data, the Distribution Coefficient as a function of salt concentration, and the height equivalent to a theoretical plate (HETP) as a function of mobile phase velocity were calculated. The optimized linear gradient elution conditions were determined based on the relationship between buffer consumption and separation time. The optimal stepwise elution conditions were determined based on the relationship between the Distribution Coefficient and the salt concentration.
S Mishra - One of the best experts on this subject based on the ideXlab platform.
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measurement of uranium Distribution Coefficient and 235u 238u ratio in soils affected by fukushima dai ichi nuclear power plant accident
Journal of Environmental Radioactivity, 2019Co-Authors: S Mishra, S Kasar, Asako Takamasa, Nimelan Veerasamy, Sarata Kumar SahooAbstract:Abstract Fukushima Daiichi Nuclear Power Plant (FDNPP) accident resulted radioactive contamination in soil due to deposition of mainly radiocesium as well as many long-lived radionuclides surrounding a large area around FDNPP. Depending upon environmental conditions, radionuclides in soil can be mobilized in aquatic systems. Therefore, the fate and transfer of these radionuclides in the soil water system is very important for radiation protection and dose assessment. In the present study, soil and water samples were collected from contaminated areas around FDNPP. Inductively coupled plasma mass spectrometry (ICP-MS) is used for total uranium concentration. Emphasis has been given on isotope ratio measurement of 235U/238U ratio using thermal ionization mass spectrometry (TIMS) that gives us the idea about its contamination during accident. For the migration behavior, its Distribution Coefficient (Kd) has been determined using laboratory batch method. Chemical characterization of soil with respect to different parameters has been carried out. The effect of these soil parameters on Distribution Coefficient of uranium has been studied in order to explain the radionuclide mobility in this particular area. The Distribution Coefficient values for uranium are found to vary from 30 to 36000 L/kg. A large variation in the Distribution Coefficient values shows the retention or mobility of uranium is highly dependent on soil characteristics in the particular area. This variation is explained with respect to soil pH, Fe, Mn, CaCO3 and organic content. There is a very good correlation of uranium Kd obtained with Fe content. There is no enrichment of 235U has been noticed in the studied area.
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Mass Spectrometric measurement of uranium isotopes and Distribution Coefficient in Fukushima contaminated soils
2019Co-Authors: Sarata Kumar Sahoo, S Mishra, 賞雅 朝子, 新江 秀樹, 青野 辰雄, Z. PalaczAbstract:Earthquake of magnitude 9.0 followed by Tsunami on 11 March 2011 caused nuclear accident at the Fukushima Daiichi Nuclear Power Plant (FDNPP). This resulted radioactive contamination due to deposition of mainly radiocesium as well as many long-lived radionuclides surrounding the area. Depending upon environmental conditions, radionuclides can be mobilized in aquatic systems. Therefore, the fate and transfer of these radionuclides in the soil water system is very important for radiation protection and dose assessment. In the present study, soil and water samples were collected from contaminated areas around FDNPP. Inductively coupled plasma mass spectrometry (ICP-MS) is used for total uranium concentration and thermal ionization mass spectrometry (TIMS) has been used for uranium isotopes measurement. Extraction chromatography has been used for the separation of uranium. Activity ratio, 234U/238U ratio measured in spiked solution by MC-ICP-MS and non-spiked aliquot using TIMS were compared. Both measured values were in good agreement within 95% accuracy. We have observed, isotope ratio 235U/238U is of natural origin, 234U/238U (activity ratio) as a base line study and in a few soil samples 236U has been detected. For the migration behavior, its Distribution Coefficient (Kd) has been determined using laboratory batch method. Chemical characterization of soil with respect to different parameters has been carried out in order to explain the radionuclide mobility in this particular area [1]. There is a variation with uranium activity ratio where as no enrichment of 235U has been noticed in the studied area. However, contaminated water used for cooling the reactor vessel stored near the reactor after accident, may contain radioactive uranium. This study will be helpful for prediction of radionuclide migration in the contaminated site.\nReferences [1] Mishra S., Sahoo S.K., Arae H., Watanabe Y., Mietelski J.W. J Chromatogr Sep Tech 5: 1000250. Doi: 10.4172/2157-7064.1000250 (2014)The 61st Annual Radiobioassay & Radiochemical Measurements Conferenceに参加し、MASS SPECTROMETRIC MEASUREMENT OF URANIUM ISOTOPES AND Distribution Coefficient IN FUKUSHIMA CONTAMINATED SOILS の口頭発表を行う
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estimation of Distribution Coefficient of natural radionuclides in soil around uranium mines and its effect with ionic strength of water
Radiation Protection Dosimetry, 2012Co-Authors: S Mishra, Sukanta Maity, G G PanditAbstract:The Distribution Coefficient, K(d) in soil is an important parameter to predict the migration of contaminants. In this study, uranium (U) and its decay products thorium (Th), radium (Ra), bismuth (Bi), lead (Pb) and polonium (Po), which may contaminate the soil and ground water around uranium mining areas, have been considered. Soil and ground water samples were collected from a proposed uranium mining site in India. The soil samples were characterised for different parameters affecting the K(d) values. The batch sorption method was employed to measure the K(d) of different radionuclides. The important factors affecting the batch method for K(d) estimation were identified and optimised. The variation of K(d) was observed with different ionic strength water samples. Results showed high K(d) values for Th(IV), Po(IV) and Pb(II) (log K(d) ∼4) and low K(d) (log K(d) ∼2-3) for U(VI), Ra(II) and Bi(III) in all three types of water with different ionic strength.
Sarata Kumar Sahoo - One of the best experts on this subject based on the ideXlab platform.
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measurement of uranium Distribution Coefficient and 235u 238u ratio in soils affected by fukushima dai ichi nuclear power plant accident
Journal of Environmental Radioactivity, 2019Co-Authors: S Mishra, S Kasar, Asako Takamasa, Nimelan Veerasamy, Sarata Kumar SahooAbstract:Abstract Fukushima Daiichi Nuclear Power Plant (FDNPP) accident resulted radioactive contamination in soil due to deposition of mainly radiocesium as well as many long-lived radionuclides surrounding a large area around FDNPP. Depending upon environmental conditions, radionuclides in soil can be mobilized in aquatic systems. Therefore, the fate and transfer of these radionuclides in the soil water system is very important for radiation protection and dose assessment. In the present study, soil and water samples were collected from contaminated areas around FDNPP. Inductively coupled plasma mass spectrometry (ICP-MS) is used for total uranium concentration. Emphasis has been given on isotope ratio measurement of 235U/238U ratio using thermal ionization mass spectrometry (TIMS) that gives us the idea about its contamination during accident. For the migration behavior, its Distribution Coefficient (Kd) has been determined using laboratory batch method. Chemical characterization of soil with respect to different parameters has been carried out. The effect of these soil parameters on Distribution Coefficient of uranium has been studied in order to explain the radionuclide mobility in this particular area. The Distribution Coefficient values for uranium are found to vary from 30 to 36000 L/kg. A large variation in the Distribution Coefficient values shows the retention or mobility of uranium is highly dependent on soil characteristics in the particular area. This variation is explained with respect to soil pH, Fe, Mn, CaCO3 and organic content. There is a very good correlation of uranium Kd obtained with Fe content. There is no enrichment of 235U has been noticed in the studied area.
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Mass Spectrometric measurement of uranium isotopes and Distribution Coefficient in Fukushima contaminated soils
2019Co-Authors: Sarata Kumar Sahoo, S Mishra, 賞雅 朝子, 新江 秀樹, 青野 辰雄, Z. PalaczAbstract:Earthquake of magnitude 9.0 followed by Tsunami on 11 March 2011 caused nuclear accident at the Fukushima Daiichi Nuclear Power Plant (FDNPP). This resulted radioactive contamination due to deposition of mainly radiocesium as well as many long-lived radionuclides surrounding the area. Depending upon environmental conditions, radionuclides can be mobilized in aquatic systems. Therefore, the fate and transfer of these radionuclides in the soil water system is very important for radiation protection and dose assessment. In the present study, soil and water samples were collected from contaminated areas around FDNPP. Inductively coupled plasma mass spectrometry (ICP-MS) is used for total uranium concentration and thermal ionization mass spectrometry (TIMS) has been used for uranium isotopes measurement. Extraction chromatography has been used for the separation of uranium. Activity ratio, 234U/238U ratio measured in spiked solution by MC-ICP-MS and non-spiked aliquot using TIMS were compared. Both measured values were in good agreement within 95% accuracy. We have observed, isotope ratio 235U/238U is of natural origin, 234U/238U (activity ratio) as a base line study and in a few soil samples 236U has been detected. For the migration behavior, its Distribution Coefficient (Kd) has been determined using laboratory batch method. Chemical characterization of soil with respect to different parameters has been carried out in order to explain the radionuclide mobility in this particular area [1]. There is a variation with uranium activity ratio where as no enrichment of 235U has been noticed in the studied area. However, contaminated water used for cooling the reactor vessel stored near the reactor after accident, may contain radioactive uranium. This study will be helpful for prediction of radionuclide migration in the contaminated site.\nReferences [1] Mishra S., Sahoo S.K., Arae H., Watanabe Y., Mietelski J.W. J Chromatogr Sep Tech 5: 1000250. Doi: 10.4172/2157-7064.1000250 (2014)The 61st Annual Radiobioassay & Radiochemical Measurements Conferenceに参加し、MASS SPECTROMETRIC MEASUREMENT OF URANIUM ISOTOPES AND Distribution Coefficient IN FUKUSHIMA CONTAMINATED SOILS の口頭発表を行う
Takashi Ishihara - One of the best experts on this subject based on the ideXlab platform.
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Optimization of elution salt concentration in stepwise elution of protein chromatography using linear gradient elution data. Reducing residual protein A by cation-exchange chromatography in monoclonal antibody purification.
Journal of Chromatography A, 2006Co-Authors: Takashi Ishihara, Toshihiko Kadoya, Naomi Endo, Shuichi YamamotoAbstract:Our simple method for optimization of the elution salt concentration in stepwise elution was applied to the actual protein separation system, which involves several difficulties such as detection of the target. As a model separation system, reducing residual protein A by cation-exchange chromatography in human monoclonal antibody (hMab) purification was chosen. We carried out linear gradient elution experiments and obtained the data for the peak salt concentration of hMab and residual protein A, respectively. An enzyme-linked immunosorbent assay was applied to the measurement of the residual protein A. From these data, we calculated the Distribution Coefficient of the hMab and the residual protein A as a function of salt concentration. The optimal salt concentration of stepwise elution to reduce the residual protein A from the hMab was determined based on the relationship between the Distribution Coefficient and the salt concentration. Using the optimized condition, we successfully performed the separation, resulting in high recovery of hMab and the elimination of residual protein A.
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optimization of monoclonal antibody purification by ion exchange chromatography application of simple methods with linear gradient elution experimental data
Journal of Chromatography A, 2005Co-Authors: Takashi Ishihara, Shuichi YamamotoAbstract:Simple methods for the optimization of ion-exchange chromatography of proteins in our previous papers were applied to cation-exchange chromatography purification of monoclonal antibodies (Mab). We carried out linear gradient elution experiments, and obtained the data for the peak salt concentration and the peak width. From these data, the Distribution Coefficient as a function of salt concentration, and the height equivalent to a theoretical plate (HETP) as a function of mobile phase velocity were calculated. The optimized linear gradient elution conditions were determined based on the relationship between buffer consumption and separation time. The optimal stepwise elution conditions were determined based on the relationship between the Distribution Coefficient and the salt concentration.
Nimelan Veerasamy - One of the best experts on this subject based on the ideXlab platform.
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measurement of uranium Distribution Coefficient and 235u 238u ratio in soils affected by fukushima dai ichi nuclear power plant accident
Journal of Environmental Radioactivity, 2019Co-Authors: S Mishra, S Kasar, Asako Takamasa, Nimelan Veerasamy, Sarata Kumar SahooAbstract:Abstract Fukushima Daiichi Nuclear Power Plant (FDNPP) accident resulted radioactive contamination in soil due to deposition of mainly radiocesium as well as many long-lived radionuclides surrounding a large area around FDNPP. Depending upon environmental conditions, radionuclides in soil can be mobilized in aquatic systems. Therefore, the fate and transfer of these radionuclides in the soil water system is very important for radiation protection and dose assessment. In the present study, soil and water samples were collected from contaminated areas around FDNPP. Inductively coupled plasma mass spectrometry (ICP-MS) is used for total uranium concentration. Emphasis has been given on isotope ratio measurement of 235U/238U ratio using thermal ionization mass spectrometry (TIMS) that gives us the idea about its contamination during accident. For the migration behavior, its Distribution Coefficient (Kd) has been determined using laboratory batch method. Chemical characterization of soil with respect to different parameters has been carried out. The effect of these soil parameters on Distribution Coefficient of uranium has been studied in order to explain the radionuclide mobility in this particular area. The Distribution Coefficient values for uranium are found to vary from 30 to 36000 L/kg. A large variation in the Distribution Coefficient values shows the retention or mobility of uranium is highly dependent on soil characteristics in the particular area. This variation is explained with respect to soil pH, Fe, Mn, CaCO3 and organic content. There is a very good correlation of uranium Kd obtained with Fe content. There is no enrichment of 235U has been noticed in the studied area.