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Yoshiya Shimada - One of the best experts on this subject based on the ideXlab platform.
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phosphorus localization and its involvement in the formation of concentrated Uranium in the renal proximal tubules of rats exposed to uranyl acetate
International Journal of Molecular Sciences, 2019Co-Authors: Shino Hommatakeda, Keisuke Kitahara, Yasuko Terada, Chiya Numako, Takanori Yoshida, Masakazu Oikawa, Toshiaki Kokubo, Yoshiya ShimadaAbstract:Although the kidneys comprise a critical target of Uranium exposure, the dynamics of renal Uranium distribution have remained obscure. Uranium is considered to function physiologically in the form of uranyl ions that have high affinity for phosphate groups. The present study applied microbeam-based elemental analysis to precisely determine the distribution of phosphorus and Uranium in the kidneys of male Wistar rats exposed to Uranium. One day after a single subcutaneous injection of uranyl acetate (2 mg/kg), areas of concentrated phosphorus were scattered in the S3 segments of the proximal tubule of the kidneys, whereas the S3 segments in control rats and in rats given a lower dose of Uranium (0.5 mg/kg) contained phosphorus without concentrated phosphorus. Areas with concentrated phosphorus contained Uranium 4- to 14-fold more than the mean Uranium concentration (126–472 vs. 33.1 ± 4.6 μg/g). The chemical form of Uranium in the concentrated phosphorus examined by XAFS was Uranium (VI), suggesting that the interaction of uranyl ions with the phosphate groups of biomolecules could be involved in the formation of Uranium concentration in the proximal tubules of kidneys in rats exposed to Uranium.
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cellular localization of Uranium in the renal proximal tubules during acute renal Uranium toxicity
Journal of Applied Toxicology, 2015Co-Authors: Shino Hommatakeda, Keisuke Kitahara, Kyoko Suzuki, Benjamin J Blyth, Noriyoshi Suya, Teruaki Konishi, Yasuko Terada, Yoshiya ShimadaAbstract:Renal toxicity is a hallmark of Uranium exposure, with Uranium accumulating specifically in the S3 segment of the proximal tubules causing tubular damage. As the distribution, concentration and dynamics of accumulated Uranium at the cellular level is not well understood, here, we report on high-resolution quantitative in situ measurements by high-energy synchrotron radiation X-ray fluorescence analysis in renal sections from a rat model of Uranium-induced acute renal toxicity. One day after subcutaneous administration of Uranium acetate to male Wistar rats at a dose of 0.5 mg Uranium kg(-1) body weight, Uranium concentration in the S3 segment of the proximal tubules was 64.9 ± 18.2 µg g(-1) , sevenfold higher than the mean renal Uranium concentration (9.7 ± 2.4 µg g(-1) ). Uranium distributed into the epithelium of the S3 segment of the proximal tubules and highly concentrated Uranium (50-fold above mean renal concentration) in micro-regions was found near the nuclei. These Uranium levels were maintained up to 8 days post-administration, despite more rapid reductions in mean renal concentration. Two weeks after Uranium administration, damaged areas were filled with regenerating tubules and morphological signs of tissue recovery, but areas of high Uranium concentration (100-fold above mean renal concentration) were still found in the epithelium of regenerating tubules. These data indicate that site-specific accumulation of Uranium in micro-regions of the S3 segment of the proximal tubules and retention of Uranium in concentrated areas during recovery are characteristics of Uranium behavior in the kidney.
Shino Hommatakeda - One of the best experts on this subject based on the ideXlab platform.
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two dimensional μxafs analysis for accumulated Uranium in kidneys of rats exposed to uranyl acetate
Radiation Physics and Chemistry, 2020Co-Authors: Shino Hommatakeda, Chiya Numako, Takanori Yoshida, Akihiro Uehara, Oki Sekizawa, Kiyofumi Nitta, Nobuaki SatoAbstract:Abstract Kidneys are critical targets of Uranium exposure, meaning that effective analytical methods for characterizing Uranium accumulated in kidneys are required in toxicological studies of Uranium. In this work, synchrotron radiation micro-X-ray fluorescence (SR-μXRF) imaging and two-dimensional μXAFS measurements were combined to analyze renal section specimens. By histochemical staining of the serial sections, the distribution of Uranium chemical condition within the Uranium-concentrated areas of the proximal tubules, which are toxic target sites of Uranium, were obtained with 1 µm × 1 µm spatial resolution.
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phosphorus localization and its involvement in the formation of concentrated Uranium in the renal proximal tubules of rats exposed to uranyl acetate
International Journal of Molecular Sciences, 2019Co-Authors: Shino Hommatakeda, Keisuke Kitahara, Yasuko Terada, Chiya Numako, Takanori Yoshida, Masakazu Oikawa, Toshiaki Kokubo, Yoshiya ShimadaAbstract:Although the kidneys comprise a critical target of Uranium exposure, the dynamics of renal Uranium distribution have remained obscure. Uranium is considered to function physiologically in the form of uranyl ions that have high affinity for phosphate groups. The present study applied microbeam-based elemental analysis to precisely determine the distribution of phosphorus and Uranium in the kidneys of male Wistar rats exposed to Uranium. One day after a single subcutaneous injection of uranyl acetate (2 mg/kg), areas of concentrated phosphorus were scattered in the S3 segments of the proximal tubule of the kidneys, whereas the S3 segments in control rats and in rats given a lower dose of Uranium (0.5 mg/kg) contained phosphorus without concentrated phosphorus. Areas with concentrated phosphorus contained Uranium 4- to 14-fold more than the mean Uranium concentration (126–472 vs. 33.1 ± 4.6 μg/g). The chemical form of Uranium in the concentrated phosphorus examined by XAFS was Uranium (VI), suggesting that the interaction of uranyl ions with the phosphate groups of biomolecules could be involved in the formation of Uranium concentration in the proximal tubules of kidneys in rats exposed to Uranium.
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cellular localization of Uranium in the renal proximal tubules during acute renal Uranium toxicity
Journal of Applied Toxicology, 2015Co-Authors: Shino Hommatakeda, Keisuke Kitahara, Kyoko Suzuki, Benjamin J Blyth, Noriyoshi Suya, Teruaki Konishi, Yasuko Terada, Yoshiya ShimadaAbstract:Renal toxicity is a hallmark of Uranium exposure, with Uranium accumulating specifically in the S3 segment of the proximal tubules causing tubular damage. As the distribution, concentration and dynamics of accumulated Uranium at the cellular level is not well understood, here, we report on high-resolution quantitative in situ measurements by high-energy synchrotron radiation X-ray fluorescence analysis in renal sections from a rat model of Uranium-induced acute renal toxicity. One day after subcutaneous administration of Uranium acetate to male Wistar rats at a dose of 0.5 mg Uranium kg(-1) body weight, Uranium concentration in the S3 segment of the proximal tubules was 64.9 ± 18.2 µg g(-1) , sevenfold higher than the mean renal Uranium concentration (9.7 ± 2.4 µg g(-1) ). Uranium distributed into the epithelium of the S3 segment of the proximal tubules and highly concentrated Uranium (50-fold above mean renal concentration) in micro-regions was found near the nuclei. These Uranium levels were maintained up to 8 days post-administration, despite more rapid reductions in mean renal concentration. Two weeks after Uranium administration, damaged areas were filled with regenerating tubules and morphological signs of tissue recovery, but areas of high Uranium concentration (100-fold above mean renal concentration) were still found in the epithelium of regenerating tubules. These data indicate that site-specific accumulation of Uranium in micro-regions of the S3 segment of the proximal tubules and retention of Uranium in concentrated areas during recovery are characteristics of Uranium behavior in the kidney.
Keisuke Kitahara - One of the best experts on this subject based on the ideXlab platform.
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phosphorus localization and its involvement in the formation of concentrated Uranium in the renal proximal tubules of rats exposed to uranyl acetate
International Journal of Molecular Sciences, 2019Co-Authors: Shino Hommatakeda, Keisuke Kitahara, Yasuko Terada, Chiya Numako, Takanori Yoshida, Masakazu Oikawa, Toshiaki Kokubo, Yoshiya ShimadaAbstract:Although the kidneys comprise a critical target of Uranium exposure, the dynamics of renal Uranium distribution have remained obscure. Uranium is considered to function physiologically in the form of uranyl ions that have high affinity for phosphate groups. The present study applied microbeam-based elemental analysis to precisely determine the distribution of phosphorus and Uranium in the kidneys of male Wistar rats exposed to Uranium. One day after a single subcutaneous injection of uranyl acetate (2 mg/kg), areas of concentrated phosphorus were scattered in the S3 segments of the proximal tubule of the kidneys, whereas the S3 segments in control rats and in rats given a lower dose of Uranium (0.5 mg/kg) contained phosphorus without concentrated phosphorus. Areas with concentrated phosphorus contained Uranium 4- to 14-fold more than the mean Uranium concentration (126–472 vs. 33.1 ± 4.6 μg/g). The chemical form of Uranium in the concentrated phosphorus examined by XAFS was Uranium (VI), suggesting that the interaction of uranyl ions with the phosphate groups of biomolecules could be involved in the formation of Uranium concentration in the proximal tubules of kidneys in rats exposed to Uranium.
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cellular localization of Uranium in the renal proximal tubules during acute renal Uranium toxicity
Journal of Applied Toxicology, 2015Co-Authors: Shino Hommatakeda, Keisuke Kitahara, Kyoko Suzuki, Benjamin J Blyth, Noriyoshi Suya, Teruaki Konishi, Yasuko Terada, Yoshiya ShimadaAbstract:Renal toxicity is a hallmark of Uranium exposure, with Uranium accumulating specifically in the S3 segment of the proximal tubules causing tubular damage. As the distribution, concentration and dynamics of accumulated Uranium at the cellular level is not well understood, here, we report on high-resolution quantitative in situ measurements by high-energy synchrotron radiation X-ray fluorescence analysis in renal sections from a rat model of Uranium-induced acute renal toxicity. One day after subcutaneous administration of Uranium acetate to male Wistar rats at a dose of 0.5 mg Uranium kg(-1) body weight, Uranium concentration in the S3 segment of the proximal tubules was 64.9 ± 18.2 µg g(-1) , sevenfold higher than the mean renal Uranium concentration (9.7 ± 2.4 µg g(-1) ). Uranium distributed into the epithelium of the S3 segment of the proximal tubules and highly concentrated Uranium (50-fold above mean renal concentration) in micro-regions was found near the nuclei. These Uranium levels were maintained up to 8 days post-administration, despite more rapid reductions in mean renal concentration. Two weeks after Uranium administration, damaged areas were filled with regenerating tubules and morphological signs of tissue recovery, but areas of high Uranium concentration (100-fold above mean renal concentration) were still found in the epithelium of regenerating tubules. These data indicate that site-specific accumulation of Uranium in micro-regions of the S3 segment of the proximal tubules and retention of Uranium in concentrated areas during recovery are characteristics of Uranium behavior in the kidney.
Yasuko Terada - One of the best experts on this subject based on the ideXlab platform.
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phosphorus localization and its involvement in the formation of concentrated Uranium in the renal proximal tubules of rats exposed to uranyl acetate
International Journal of Molecular Sciences, 2019Co-Authors: Shino Hommatakeda, Keisuke Kitahara, Yasuko Terada, Chiya Numako, Takanori Yoshida, Masakazu Oikawa, Toshiaki Kokubo, Yoshiya ShimadaAbstract:Although the kidneys comprise a critical target of Uranium exposure, the dynamics of renal Uranium distribution have remained obscure. Uranium is considered to function physiologically in the form of uranyl ions that have high affinity for phosphate groups. The present study applied microbeam-based elemental analysis to precisely determine the distribution of phosphorus and Uranium in the kidneys of male Wistar rats exposed to Uranium. One day after a single subcutaneous injection of uranyl acetate (2 mg/kg), areas of concentrated phosphorus were scattered in the S3 segments of the proximal tubule of the kidneys, whereas the S3 segments in control rats and in rats given a lower dose of Uranium (0.5 mg/kg) contained phosphorus without concentrated phosphorus. Areas with concentrated phosphorus contained Uranium 4- to 14-fold more than the mean Uranium concentration (126–472 vs. 33.1 ± 4.6 μg/g). The chemical form of Uranium in the concentrated phosphorus examined by XAFS was Uranium (VI), suggesting that the interaction of uranyl ions with the phosphate groups of biomolecules could be involved in the formation of Uranium concentration in the proximal tubules of kidneys in rats exposed to Uranium.
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cellular localization of Uranium in the renal proximal tubules during acute renal Uranium toxicity
Journal of Applied Toxicology, 2015Co-Authors: Shino Hommatakeda, Keisuke Kitahara, Kyoko Suzuki, Benjamin J Blyth, Noriyoshi Suya, Teruaki Konishi, Yasuko Terada, Yoshiya ShimadaAbstract:Renal toxicity is a hallmark of Uranium exposure, with Uranium accumulating specifically in the S3 segment of the proximal tubules causing tubular damage. As the distribution, concentration and dynamics of accumulated Uranium at the cellular level is not well understood, here, we report on high-resolution quantitative in situ measurements by high-energy synchrotron radiation X-ray fluorescence analysis in renal sections from a rat model of Uranium-induced acute renal toxicity. One day after subcutaneous administration of Uranium acetate to male Wistar rats at a dose of 0.5 mg Uranium kg(-1) body weight, Uranium concentration in the S3 segment of the proximal tubules was 64.9 ± 18.2 µg g(-1) , sevenfold higher than the mean renal Uranium concentration (9.7 ± 2.4 µg g(-1) ). Uranium distributed into the epithelium of the S3 segment of the proximal tubules and highly concentrated Uranium (50-fold above mean renal concentration) in micro-regions was found near the nuclei. These Uranium levels were maintained up to 8 days post-administration, despite more rapid reductions in mean renal concentration. Two weeks after Uranium administration, damaged areas were filled with regenerating tubules and morphological signs of tissue recovery, but areas of high Uranium concentration (100-fold above mean renal concentration) were still found in the epithelium of regenerating tubules. These data indicate that site-specific accumulation of Uranium in micro-regions of the S3 segment of the proximal tubules and retention of Uranium in concentrated areas during recovery are characteristics of Uranium behavior in the kidney.
N Shikazono - One of the best experts on this subject based on the ideXlab platform.
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stable isotopes minor elements and diagenetic alteration associated with tono sandstone type Uranium deposits in japan
Mineralogical Magazine, 1994Co-Authors: N ShikazonoAbstract:Sandstone-type Uranium deposits are the most important Uranium producers among various types of Uranium deposits. Geologic setting and diagenetic alteration mineralogy of this type of deposits have been well documented. The genesis of sandstone-type Uranium deposits have been argued based on geologic and mineralogic studies (Nash et al., 1981). However, it is essentially important to estimate chemical features (Eh, pH) and isotopic compositions of fluids responsible for Uranium mineralization and associated diagenetic alteration in order to elucidate genesis of the Uranium deposits and diagenetic alteration mechanism. A few such geochemical studies on the sandstone-type deposits have been carried out. In this study, the geochemical features of the diagenetic alteration in the Tone mine area are used to provide constraints on the relationship between Uranium mineralization and diagenetic alteration process and the estimation of chemical environments responsible for Uranium mineralization.
