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Gérard Duvallet - One of the best experts on this subject based on the ideXlab platform.
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microsatellite loci in the stable fly stomoxys niger niger diptera muscidae on la reunion island
Molecular Ecology Notes, 2005Co-Authors: Jeremie R. L. Gilles, Isabelle Litrico, Gérard DuvalletAbstract:Six polymorphic microsatellite markers have been isolated from a microsatellite-enriched DNA Library from the stable fly, Stomoxys niger niger. These loci exhibited five to 10 alleles per locus and an expected heterozygosity ranging from 0.57 to 0.81 in the studied populations from La Reunion Island (Indian Ocean). They should therefore be useful for the study of genetic diversity and population structure of S. niger. In addition, cross-species amplification of four of these six loci in the closely allied species Stomoxys calcitrans produced interpretable results, two of which would be useful for population biology studies. [Resume d'auteur]
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Microsatellite DNA markers for the Stable Fly, Stomoxys calcitrans (Diptera: Muscidae)
Molecular Ecology Notes, 2004Co-Authors: Jeremie R. L. Gilles, Isabelle Litrico, P. Sourrouille, Gérard DuvalletAbstract:Eight polymorphic microsatellite markers have been isolated from a microsatellite-enriched DNA Library from the Stable Fly, Stomoxys calcitrans . These loci exhibited four to 15 alleles per locus and an expected heterozygosity ranging from 0.19 to 0.84 in the three populations studied from La Reunion island (Indian Ocean). They should therefore be valuable for studying genetic diversity and population structure. Cross-species amplification of these eight loci in the closely allied species Stomoxys niger niger was successful for four of these loci, one of which was monomorphic and one of which strongly departed from Hardy– Weinberg expectations.
Paul Chalhoub - One of the best experts on this subject based on the ideXlab platform.
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High-resolution mapping and chromosome landing at the root-knot nematode resistance locus Ma from Myrobalan plum using a large-insert BAC DNA Library
TAG Theoretical and Applied Genetics, 2004Co-Authors: Michel Claverie, Elisabeth Dirlewanger, Patrick Cosson, Nathalie Bosselut, C. Lecouls, Roger Voisin, Marc Kleinhentz, Bernard Lafargue, Michel Caboche, Paul ChalhoubAbstract:The Ma gene for root-knot nematode (RKN) resistance from Myrobalan plum (Prunus cerasifera L.) confers a complete-spectrum and a heat-stable resistance to Meloidogyne spp., conversely to Mi-1 from tomato, which has a more restricted spectrum and a reduced efficiency at high temperature. This gene was identified from a perennial self-incompatible near-wild rootstock species and lies in cosegregation with the SCAR marker SCAFLP2 on the Prunus linkage group 7 in a 2.3 cM interval between the SCAR SCAL19 and SSR pchgms6 markers. We initiated a map-based cloning of Ma and report here the strategy that rapidly led to fine mapping and direct chromosome landing at the locus. Three pairs of bulks, totaling 90 individuals from half-sibling progenies derived from the Ma-heterozygous resistant accession P.2175, were constructed using mapping data, and saturation of the Ma region was performed by bulked segregant analysis (BSA) of 320 AFLP primer pair combinations. The closest three AFLP markers were transformed into codominant SCARs or CAPS designated SCAFLP3, SCAFLP4 and SCAFLP5. By completing the mapping population up to 1,332 offspring from P.2175, Ma and SCAFLP2 were mapped in a 0.8 cM interval between SCAFLP3 and SCAFLP4. A large-insert bacterial artificial chromosome (BAC) DNA Library of P.2175, totaling 30,720 clones with a mean insert size of 145 kb and a 14-15x Prunus haploid genome coverage was constructed and used to land on the Ma spanning interval with few BAC clones. As P.2175 is heterozygous for the gene, we constructed the resistant and susceptible physical contigs by PCR screening of the Library with codominant markers. Additional microsatellite markers were then designed from BAC subcloning or BAC end sequencing. In the resistant contig, a single 280 kb BAC clone was shown to carry the Ma gene; this BAC contains two flanking markers on each side of the gene as well as two cosegregating markers. These results should allow future cloning of the Ma gene in this perennial species.
Jeremie R. L. Gilles - One of the best experts on this subject based on the ideXlab platform.
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microsatellite loci in the stable fly stomoxys niger niger diptera muscidae on la reunion island
Molecular Ecology Notes, 2005Co-Authors: Jeremie R. L. Gilles, Isabelle Litrico, Gérard DuvalletAbstract:Six polymorphic microsatellite markers have been isolated from a microsatellite-enriched DNA Library from the stable fly, Stomoxys niger niger. These loci exhibited five to 10 alleles per locus and an expected heterozygosity ranging from 0.57 to 0.81 in the studied populations from La Reunion Island (Indian Ocean). They should therefore be useful for the study of genetic diversity and population structure of S. niger. In addition, cross-species amplification of four of these six loci in the closely allied species Stomoxys calcitrans produced interpretable results, two of which would be useful for population biology studies. [Resume d'auteur]
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Microsatellite DNA markers for the Stable Fly, Stomoxys calcitrans (Diptera: Muscidae)
Molecular Ecology Notes, 2004Co-Authors: Jeremie R. L. Gilles, Isabelle Litrico, P. Sourrouille, Gérard DuvalletAbstract:Eight polymorphic microsatellite markers have been isolated from a microsatellite-enriched DNA Library from the Stable Fly, Stomoxys calcitrans . These loci exhibited four to 15 alleles per locus and an expected heterozygosity ranging from 0.19 to 0.84 in the three populations studied from La Reunion island (Indian Ocean). They should therefore be valuable for studying genetic diversity and population structure. Cross-species amplification of these eight loci in the closely allied species Stomoxys niger niger was successful for four of these loci, one of which was monomorphic and one of which strongly departed from Hardy– Weinberg expectations.
Isabelle Litrico - One of the best experts on this subject based on the ideXlab platform.
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microsatellite loci in the stable fly stomoxys niger niger diptera muscidae on la reunion island
Molecular Ecology Notes, 2005Co-Authors: Jeremie R. L. Gilles, Isabelle Litrico, Gérard DuvalletAbstract:Six polymorphic microsatellite markers have been isolated from a microsatellite-enriched DNA Library from the stable fly, Stomoxys niger niger. These loci exhibited five to 10 alleles per locus and an expected heterozygosity ranging from 0.57 to 0.81 in the studied populations from La Reunion Island (Indian Ocean). They should therefore be useful for the study of genetic diversity and population structure of S. niger. In addition, cross-species amplification of four of these six loci in the closely allied species Stomoxys calcitrans produced interpretable results, two of which would be useful for population biology studies. [Resume d'auteur]
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Microsatellite DNA markers for the Stable Fly, Stomoxys calcitrans (Diptera: Muscidae)
Molecular Ecology Notes, 2004Co-Authors: Jeremie R. L. Gilles, Isabelle Litrico, P. Sourrouille, Gérard DuvalletAbstract:Eight polymorphic microsatellite markers have been isolated from a microsatellite-enriched DNA Library from the Stable Fly, Stomoxys calcitrans . These loci exhibited four to 15 alleles per locus and an expected heterozygosity ranging from 0.19 to 0.84 in the three populations studied from La Reunion island (Indian Ocean). They should therefore be valuable for studying genetic diversity and population structure. Cross-species amplification of these eight loci in the closely allied species Stomoxys niger niger was successful for four of these loci, one of which was monomorphic and one of which strongly departed from Hardy– Weinberg expectations.
Michel Claverie - One of the best experts on this subject based on the ideXlab platform.
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High-resolution mapping and chromosome landing at the root-knot nematode resistance locus Ma from Myrobalan plum using a large-insert BAC DNA Library
TAG Theoretical and Applied Genetics, 2004Co-Authors: Michel Claverie, Elisabeth Dirlewanger, Patrick Cosson, Nathalie Bosselut, C. Lecouls, Roger Voisin, Marc Kleinhentz, Bernard Lafargue, Michel Caboche, Paul ChalhoubAbstract:The Ma gene for root-knot nematode (RKN) resistance from Myrobalan plum (Prunus cerasifera L.) confers a complete-spectrum and a heat-stable resistance to Meloidogyne spp., conversely to Mi-1 from tomato, which has a more restricted spectrum and a reduced efficiency at high temperature. This gene was identified from a perennial self-incompatible near-wild rootstock species and lies in cosegregation with the SCAR marker SCAFLP2 on the Prunus linkage group 7 in a 2.3 cM interval between the SCAR SCAL19 and SSR pchgms6 markers. We initiated a map-based cloning of Ma and report here the strategy that rapidly led to fine mapping and direct chromosome landing at the locus. Three pairs of bulks, totaling 90 individuals from half-sibling progenies derived from the Ma-heterozygous resistant accession P.2175, were constructed using mapping data, and saturation of the Ma region was performed by bulked segregant analysis (BSA) of 320 AFLP primer pair combinations. The closest three AFLP markers were transformed into codominant SCARs or CAPS designated SCAFLP3, SCAFLP4 and SCAFLP5. By completing the mapping population up to 1,332 offspring from P.2175, Ma and SCAFLP2 were mapped in a 0.8 cM interval between SCAFLP3 and SCAFLP4. A large-insert bacterial artificial chromosome (BAC) DNA Library of P.2175, totaling 30,720 clones with a mean insert size of 145 kb and a 14-15x Prunus haploid genome coverage was constructed and used to land on the Ma spanning interval with few BAC clones. As P.2175 is heterozygous for the gene, we constructed the resistant and susceptible physical contigs by PCR screening of the Library with codominant markers. Additional microsatellite markers were then designed from BAC subcloning or BAC end sequencing. In the resistant contig, a single 280 kb BAC clone was shown to carry the Ma gene; this BAC contains two flanking markers on each side of the gene as well as two cosegregating markers. These results should allow future cloning of the Ma gene in this perennial species.