The Experts below are selected from a list of 18 Experts worldwide ranked by ideXlab platform
Hubert Motschmann - One of the best experts on this subject based on the ideXlab platform.
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the influence of lanthanide iii nitrates on adsorbed monolayers of Dodecylphosphorylcholine at the air water interface
Journal of Colloid and Interface Science, 2019Co-Authors: Matthias J Hofmann, Epameinondas Leontidis, Hubert MotschmannAbstract:Abstract Adsorption layers at the air-water interface formed from the soluble zwitterionic surfactant Dodecylphosphorylcholine (DPC) serving as a soluble model substance for phospho-lipids were characterized with respect to their equilibrium and dynamic surface properties. To clarify the effect of ionic interactions with electrolyte present in the bulk phase, surface rheological properties upon addition of Ce(NO3)3 and Yb(NO3)3 were determined. In order to account for the surface activity of the nitrate ion, comparative measurements using NaNO3 were carried out additionally. Further experimental information on the bulk hydration characteristics of the aqueous solutions was obtained using dielectric relaxation spectroscopy (DRS). A possible mechanism causing the deviating surface dilatational modulus E in terms of ion specificity is suggested.
Matthias J Hofmann - One of the best experts on this subject based on the ideXlab platform.
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the influence of lanthanide iii nitrates on adsorbed monolayers of Dodecylphosphorylcholine at the air water interface
Journal of Colloid and Interface Science, 2019Co-Authors: Matthias J Hofmann, Epameinondas Leontidis, Hubert MotschmannAbstract:Abstract Adsorption layers at the air-water interface formed from the soluble zwitterionic surfactant Dodecylphosphorylcholine (DPC) serving as a soluble model substance for phospho-lipids were characterized with respect to their equilibrium and dynamic surface properties. To clarify the effect of ionic interactions with electrolyte present in the bulk phase, surface rheological properties upon addition of Ce(NO3)3 and Yb(NO3)3 were determined. In order to account for the surface activity of the nitrate ion, comparative measurements using NaNO3 were carried out additionally. Further experimental information on the bulk hydration characteristics of the aqueous solutions was obtained using dielectric relaxation spectroscopy (DRS). A possible mechanism causing the deviating surface dilatational modulus E in terms of ion specificity is suggested.
Koji Tomoo - One of the best experts on this subject based on the ideXlab platform.
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x ray crystal structure and molecular dynamics simulation of bovine pancreas phospholipase a2 n Dodecylphosphorylcholine complex
Proteins, 1994Co-Authors: Koji Tomoo, Hirofumi Ohishi, Toshimasa Ishida, Masatoshi Inoue, Kiyoshi Ikeda, Shigeyuki Sumiya, Kunihiro KitamuraAbstract:The crystal structure of n-Dodecylphosphorylcholine (n-C12PC)-bovine pancreas phospholipase A2 (PLA2) complex provided the following structural characteristics: (1) the dodecyl chain of n-C12PC was located at the PLA2 N-terminal helical region by hydrophobic interactions, which corresponds to the binding pocket of 2-acyl fatty acid chain (beta-chain) of the substrate phospholipid, (2) the region from Lys-53 to Lys-56 creates a choline-receiving pocket of n-C12PC and (3) the N-terminal group of Ala-1 shifts significantly toward the Tyr-52 OH group by the binding of the n-C12PC inhibitor. Since the accuracy of the X-ray analysis (R = 0.275 at 2.3 A resolution) was insufficient to establish these important X-ray insights, the complex structure was further investigated through the molecular dynamics (MD) simulation, assuming a system in aqueous solution at 310K. The MD simulation covering 176 ps showed that the structural characteristics observed by X-ray analysis are intrinsic and also stable in the dynamic state. Furthermore, the MD simulation made clear that the PLA2 binding pocket is large enough to permit the conformational fluctuation of the n-C12PC hydrocarbon chain.
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interaction mode of n Dodecylphosphorylcholine a substrate analogue with bovine pancreas phospholipase a2 as determined by x ray crystal analysis
Biochemical and Biophysical Research Communications, 1992Co-Authors: Koji Tomoo, Hirofumi Ohishi, Mitsunobu Doi, Toshimasa Ishida, Masatoshi Inoue, Kiyoshi Ikeda, Hiroshi MizunoAbstract:Abstract Three-dimensional structure of a bovine pancreas phospholipase A 2 (PLA 2 ) crystal complexed with n -Dodecylphosphorylcholine ( n -C 12 PC), a substrate-type inhibitor, has been determined by the X-ray diffraction method. The present conventional R value is 0.275 at 2.3A resolution. The binding mode of n -C 12 PC to the PLA 2 was clearly indicated, where the dodecyl chain was stably held by the hydrophobic contacts with the N-terminal rigion of PLA 2 (Leu-2, Phe-5, and Ile-9), and the choline moiety was contacted with the hydrophobic space created by the side chains of Lys-53 and 56. The present result indicates that remarkable changes from the native PLA 2 structure are caused at the N-terminal and middle (residues 60 to 70) regions by the binding of n -C 12 PC to the enzyme.
Hiroshi Mizuno - One of the best experts on this subject based on the ideXlab platform.
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interaction mode of n Dodecylphosphorylcholine a substrate analogue with bovine pancreas phospholipase a2 as determined by x ray crystal analysis
Biochemical and Biophysical Research Communications, 1992Co-Authors: Koji Tomoo, Hirofumi Ohishi, Mitsunobu Doi, Toshimasa Ishida, Masatoshi Inoue, Kiyoshi Ikeda, Hiroshi MizunoAbstract:Abstract Three-dimensional structure of a bovine pancreas phospholipase A 2 (PLA 2 ) crystal complexed with n -Dodecylphosphorylcholine ( n -C 12 PC), a substrate-type inhibitor, has been determined by the X-ray diffraction method. The present conventional R value is 0.275 at 2.3A resolution. The binding mode of n -C 12 PC to the PLA 2 was clearly indicated, where the dodecyl chain was stably held by the hydrophobic contacts with the N-terminal rigion of PLA 2 (Leu-2, Phe-5, and Ile-9), and the choline moiety was contacted with the hydrophobic space created by the side chains of Lys-53 and 56. The present result indicates that remarkable changes from the native PLA 2 structure are caused at the N-terminal and middle (residues 60 to 70) regions by the binding of n -C 12 PC to the enzyme.
Epameinondas Leontidis - One of the best experts on this subject based on the ideXlab platform.
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the influence of lanthanide iii nitrates on adsorbed monolayers of Dodecylphosphorylcholine at the air water interface
Journal of Colloid and Interface Science, 2019Co-Authors: Matthias J Hofmann, Epameinondas Leontidis, Hubert MotschmannAbstract:Abstract Adsorption layers at the air-water interface formed from the soluble zwitterionic surfactant Dodecylphosphorylcholine (DPC) serving as a soluble model substance for phospho-lipids were characterized with respect to their equilibrium and dynamic surface properties. To clarify the effect of ionic interactions with electrolyte present in the bulk phase, surface rheological properties upon addition of Ce(NO3)3 and Yb(NO3)3 were determined. In order to account for the surface activity of the nitrate ion, comparative measurements using NaNO3 were carried out additionally. Further experimental information on the bulk hydration characteristics of the aqueous solutions was obtained using dielectric relaxation spectroscopy (DRS). A possible mechanism causing the deviating surface dilatational modulus E in terms of ion specificity is suggested.