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Akio Kobayashi - One of the best experts on this subject based on the ideXlab platform.
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separation of polyprenol and Dolichol by monolithic silica capillary column chromatography
Journal of Lipid Research, 2005Co-Authors: Takeshi Bamba, Eiiciro Fukusaki, Hiroshi Minakuchi, Yoshihisa Nakazawa, Akio KobayashiAbstract:We attempted an analysis of naturally occurring polyprenol and Dolichol using a monolithic silica capillary column in HPLC. First, the separation of the polyprenol mix- ture alone was performed using a 250 � 0.2 mm inner diam- eter (ID) octadecylsilyl (ODS)-monolithic silica capillary col- umn. The resolution of the separation between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded by � 2-fold the level recorded when using a conventional ODS- silica particle-packed column (250 � 4.6 mm ID) under the same elution conditions. Next, the mixture of the prenol type (polyprenol) and Dolichol type (dihydropolyprenol) was sub- jected to this capillary HPLC system, and the separation of each homolog was successfully achieved. During the analy- sis of polyprenol fraction derived from Eucommia ulmoides leaves, Dolichols were found as a single peak, including all- trans -polyprenol and cis -polyprenol previously identified. This sensitive high-resolution system is very useful for the analysis of compounds that are structurally close to polypre- nols and Dolichols and that have a low content. —Bamba, T., E. Fukusaki, H. Minakuchi, Y. Nakazawa, and A. Kobayashi. Separation of polyprenol and Dolichol by monolithic silica capillary column chromatography. J. Lipid Res. 2005. 46: 2295- 2298.
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separation of polyprenol and Dolichol by monolithic silica capillary column chromatography
Journal of Lipid Research, 2005Co-Authors: Takeshi Bamba, Eiiciro Fukusaki, Hiroshi Minakuchi, Yoshihisa Nakazawa, Akio KobayashiAbstract:We attempted an analysis of naturally occurring polyprenol and Dolichol using a monolithic silica capillary column in HPLC. First, the separation of the polyprenol mix- ture alone was performed using a 250 � 0.2 mm inner diam- eter (ID) octadecylsilyl (ODS)-monolithic silica capillary col- umn. The resolution of the separation between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded by � 2-fold the level recorded when using a conventional ODS- silica particle-packed column (250 � 4.6 mm ID) under the same elution conditions. Next, the mixture of the prenol type (polyprenol) and Dolichol type (dihydropolyprenol) was sub- jected to this capillary HPLC system, and the separation of each homolog was successfully achieved. During the analy- sis of polyprenol fraction derived from Eucommia ulmoides leaves, Dolichols were found as a single peak, including all- trans -polyprenol and cis -polyprenol previously identified. This sensitive high-resolution system is very useful for the analysis of compounds that are structurally close to polypre- nols and Dolichols and that have a low content. —Bamba, T., E. Fukusaki, H. Minakuchi, Y. Nakazawa, and A. Kobayashi. Separation of polyprenol and Dolichol by monolithic silica capillary column chromatography. J. Lipid Res. 2005. 46: 2295- 2298.
Takeshi Bamba - One of the best experts on this subject based on the ideXlab platform.
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separation of polyprenol and Dolichol by monolithic silica capillary column chromatography
Journal of Lipid Research, 2005Co-Authors: Takeshi Bamba, Eiiciro Fukusaki, Hiroshi Minakuchi, Yoshihisa Nakazawa, Akio KobayashiAbstract:We attempted an analysis of naturally occurring polyprenol and Dolichol using a monolithic silica capillary column in HPLC. First, the separation of the polyprenol mix- ture alone was performed using a 250 � 0.2 mm inner diam- eter (ID) octadecylsilyl (ODS)-monolithic silica capillary col- umn. The resolution of the separation between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded by � 2-fold the level recorded when using a conventional ODS- silica particle-packed column (250 � 4.6 mm ID) under the same elution conditions. Next, the mixture of the prenol type (polyprenol) and Dolichol type (dihydropolyprenol) was sub- jected to this capillary HPLC system, and the separation of each homolog was successfully achieved. During the analy- sis of polyprenol fraction derived from Eucommia ulmoides leaves, Dolichols were found as a single peak, including all- trans -polyprenol and cis -polyprenol previously identified. This sensitive high-resolution system is very useful for the analysis of compounds that are structurally close to polypre- nols and Dolichols and that have a low content. —Bamba, T., E. Fukusaki, H. Minakuchi, Y. Nakazawa, and A. Kobayashi. Separation of polyprenol and Dolichol by monolithic silica capillary column chromatography. J. Lipid Res. 2005. 46: 2295- 2298.
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separation of polyprenol and Dolichol by monolithic silica capillary column chromatography
Journal of Lipid Research, 2005Co-Authors: Takeshi Bamba, Eiiciro Fukusaki, Hiroshi Minakuchi, Yoshihisa Nakazawa, Akio KobayashiAbstract:We attempted an analysis of naturally occurring polyprenol and Dolichol using a monolithic silica capillary column in HPLC. First, the separation of the polyprenol mix- ture alone was performed using a 250 � 0.2 mm inner diam- eter (ID) octadecylsilyl (ODS)-monolithic silica capillary col- umn. The resolution of the separation between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded by � 2-fold the level recorded when using a conventional ODS- silica particle-packed column (250 � 4.6 mm ID) under the same elution conditions. Next, the mixture of the prenol type (polyprenol) and Dolichol type (dihydropolyprenol) was sub- jected to this capillary HPLC system, and the separation of each homolog was successfully achieved. During the analy- sis of polyprenol fraction derived from Eucommia ulmoides leaves, Dolichols were found as a single peak, including all- trans -polyprenol and cis -polyprenol previously identified. This sensitive high-resolution system is very useful for the analysis of compounds that are structurally close to polypre- nols and Dolichols and that have a low content. —Bamba, T., E. Fukusaki, H. Minakuchi, Y. Nakazawa, and A. Kobayashi. Separation of polyprenol and Dolichol by monolithic silica capillary column chromatography. J. Lipid Res. 2005. 46: 2295- 2298.
Mieko Oshima - One of the best experts on this subject based on the ideXlab platform.
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major defect of carbohydrate deficient glycoprotein syndrome is not found in the synthesis of dolichyl phosphate or n acetylglucosaminyl pyrophosphoryl Dolichol
Biochemical and Biophysical Research Communications, 1994Co-Authors: Etsuko Yasugi, M Nakasuji, Taeko Dohi, Mieko OshimaAbstract:The contents of dolichyl phosphate and UDP-N-acetylglucosamine:dolichyl phosphate N-acetylglucosamine 1-phosphate transferase (GlcNAc-1-P transferase) activity in fibroblasts from patients with carbohydrate-deficient-glycoprotein (CDG) syndrome were analyzed. The amount of dolichyl phosphate and GlcNAc-1-P transferase activity in CDG syndrome fibroblasts were similar to those in normal fibroblasts, suggesting that CDG syndrome may not be due to a deficiency of a biosynthetic enzyme for Dolichol-oligosaccharide intermediates, but to a metabolic error in assembly of asparagine-linked oligosaccharide.
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sequential microanalyses of free Dolichol dolichyl fatty acid ester and dolichyl phosphate levels in human serum
Biochimica et Biophysica Acta, 1994Co-Authors: Etsuko Yasugi, Mieko OshimaAbstract:Abstract Sequential microanalyses of free Dolichol, dolichyl fatty acid ester and dolichyl phosphate in human serum were made. To determine the level of each Dolichol, samples were pretreated using three different methods prior to fluorescent derivatization. To estimate the concentrations of free Dolichol, samples were added to alkaline methanol and kept at room temperature for 1 h. In case of dolichyl fatty acid ester, samples were saponified at 100°C for 1 h. To estimate dolichyl phosphate, saponified lipid extracts were treated with acid phosphatase. Each pretreated Dolichol was reacted with anthracene-9-carboxylic acid and amounts of 9-anthroyl derivatives were determined fluorometrically by HPLC. This method is simple and three types of Dolichols can be estimated using the same HPLC system. This analysis is also sufficiently sensitive for measurement of serum Dolichol levels. The contents of free Dolichol, dolichyl fatty acid ester and dolichyl phosphate in human serum were found to be 44.9 ± 10.5 ng/ml (n = 32), 76.4 ± 24.2 ng/ml (n = 32) and 43.5 ± 15.1 ng/ml (n = 13), respectively. These levels had apparently no correlation to age or serum total cholesterols. A linear correlation between Dolichols and high-density lipoprotein cholesterols reflects the fact that the Dolichols are associated with the high-density lipoprotein fraction.
Eiiciro Fukusaki - One of the best experts on this subject based on the ideXlab platform.
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separation of polyprenol and Dolichol by monolithic silica capillary column chromatography
Journal of Lipid Research, 2005Co-Authors: Takeshi Bamba, Eiiciro Fukusaki, Hiroshi Minakuchi, Yoshihisa Nakazawa, Akio KobayashiAbstract:We attempted an analysis of naturally occurring polyprenol and Dolichol using a monolithic silica capillary column in HPLC. First, the separation of the polyprenol mix- ture alone was performed using a 250 � 0.2 mm inner diam- eter (ID) octadecylsilyl (ODS)-monolithic silica capillary col- umn. The resolution of the separation between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded by � 2-fold the level recorded when using a conventional ODS- silica particle-packed column (250 � 4.6 mm ID) under the same elution conditions. Next, the mixture of the prenol type (polyprenol) and Dolichol type (dihydropolyprenol) was sub- jected to this capillary HPLC system, and the separation of each homolog was successfully achieved. During the analy- sis of polyprenol fraction derived from Eucommia ulmoides leaves, Dolichols were found as a single peak, including all- trans -polyprenol and cis -polyprenol previously identified. This sensitive high-resolution system is very useful for the analysis of compounds that are structurally close to polypre- nols and Dolichols and that have a low content. —Bamba, T., E. Fukusaki, H. Minakuchi, Y. Nakazawa, and A. Kobayashi. Separation of polyprenol and Dolichol by monolithic silica capillary column chromatography. J. Lipid Res. 2005. 46: 2295- 2298.
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separation of polyprenol and Dolichol by monolithic silica capillary column chromatography
Journal of Lipid Research, 2005Co-Authors: Takeshi Bamba, Eiiciro Fukusaki, Hiroshi Minakuchi, Yoshihisa Nakazawa, Akio KobayashiAbstract:We attempted an analysis of naturally occurring polyprenol and Dolichol using a monolithic silica capillary column in HPLC. First, the separation of the polyprenol mix- ture alone was performed using a 250 � 0.2 mm inner diam- eter (ID) octadecylsilyl (ODS)-monolithic silica capillary col- umn. The resolution of the separation between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded by � 2-fold the level recorded when using a conventional ODS- silica particle-packed column (250 � 4.6 mm ID) under the same elution conditions. Next, the mixture of the prenol type (polyprenol) and Dolichol type (dihydropolyprenol) was sub- jected to this capillary HPLC system, and the separation of each homolog was successfully achieved. During the analy- sis of polyprenol fraction derived from Eucommia ulmoides leaves, Dolichols were found as a single peak, including all- trans -polyprenol and cis -polyprenol previously identified. This sensitive high-resolution system is very useful for the analysis of compounds that are structurally close to polypre- nols and Dolichols and that have a low content. —Bamba, T., E. Fukusaki, H. Minakuchi, Y. Nakazawa, and A. Kobayashi. Separation of polyprenol and Dolichol by monolithic silica capillary column chromatography. J. Lipid Res. 2005. 46: 2295- 2298.
Hiroshi Minakuchi - One of the best experts on this subject based on the ideXlab platform.
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separation of polyprenol and Dolichol by monolithic silica capillary column chromatography
Journal of Lipid Research, 2005Co-Authors: Takeshi Bamba, Eiiciro Fukusaki, Hiroshi Minakuchi, Yoshihisa Nakazawa, Akio KobayashiAbstract:We attempted an analysis of naturally occurring polyprenol and Dolichol using a monolithic silica capillary column in HPLC. First, the separation of the polyprenol mix- ture alone was performed using a 250 � 0.2 mm inner diam- eter (ID) octadecylsilyl (ODS)-monolithic silica capillary col- umn. The resolution of the separation between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded by � 2-fold the level recorded when using a conventional ODS- silica particle-packed column (250 � 4.6 mm ID) under the same elution conditions. Next, the mixture of the prenol type (polyprenol) and Dolichol type (dihydropolyprenol) was sub- jected to this capillary HPLC system, and the separation of each homolog was successfully achieved. During the analy- sis of polyprenol fraction derived from Eucommia ulmoides leaves, Dolichols were found as a single peak, including all- trans -polyprenol and cis -polyprenol previously identified. This sensitive high-resolution system is very useful for the analysis of compounds that are structurally close to polypre- nols and Dolichols and that have a low content. —Bamba, T., E. Fukusaki, H. Minakuchi, Y. Nakazawa, and A. Kobayashi. Separation of polyprenol and Dolichol by monolithic silica capillary column chromatography. J. Lipid Res. 2005. 46: 2295- 2298.
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separation of polyprenol and Dolichol by monolithic silica capillary column chromatography
Journal of Lipid Research, 2005Co-Authors: Takeshi Bamba, Eiiciro Fukusaki, Hiroshi Minakuchi, Yoshihisa Nakazawa, Akio KobayashiAbstract:We attempted an analysis of naturally occurring polyprenol and Dolichol using a monolithic silica capillary column in HPLC. First, the separation of the polyprenol mix- ture alone was performed using a 250 � 0.2 mm inner diam- eter (ID) octadecylsilyl (ODS)-monolithic silica capillary col- umn. The resolution of the separation between octadecaprenol (prenol 18) and nonadecaprenol (prenol 19) exceeded by � 2-fold the level recorded when using a conventional ODS- silica particle-packed column (250 � 4.6 mm ID) under the same elution conditions. Next, the mixture of the prenol type (polyprenol) and Dolichol type (dihydropolyprenol) was sub- jected to this capillary HPLC system, and the separation of each homolog was successfully achieved. During the analy- sis of polyprenol fraction derived from Eucommia ulmoides leaves, Dolichols were found as a single peak, including all- trans -polyprenol and cis -polyprenol previously identified. This sensitive high-resolution system is very useful for the analysis of compounds that are structurally close to polypre- nols and Dolichols and that have a low content. —Bamba, T., E. Fukusaki, H. Minakuchi, Y. Nakazawa, and A. Kobayashi. Separation of polyprenol and Dolichol by monolithic silica capillary column chromatography. J. Lipid Res. 2005. 46: 2295- 2298.