The Experts below are selected from a list of 567 Experts worldwide ranked by ideXlab platform
David L. Kaplan - One of the best experts on this subject based on the ideXlab platform.
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tensan silk inspired hierarchical fibers for smart textile applications
ACS Nano, 2018Co-Authors: Jiajia Zhong, Yuzhao Tang, Chao Ye, Markus J. Buehler, Shengjie Ling, Wenwen Zhang, Ke Zheng, David L. KaplanAbstract:Tensan silk, a natural fiber produced by the Japanese oak silk moth (Antherea yamamai, abbreviated to A. yamamai), features superior characteristics, such as compressive elasticity and chemical resistance, when compared to the more common silk produced from the Domesticated Silkworm, Bombyx mori (B. mori). In this study, the “structure-property” relationships within A. yamamai silk are disclosed from the different structural hierarchies, confirming the outstanding toughness as dominated by the unique mesoscale fibrillar architectures. Inspired by this hierarchical construction, we fabricated A. yamamai silk-like regenerated B. mori silk fibers (RBSFs) with mechanical properties (extensibility and modulus) comparable to natural A. yamamai silk. These RBSFs were further functionalized to form conductive RBSFs that were sensitive to force and temperature stimuli for applications in smart textiles. This study provides a blueprint in exploiting novel designs from one type of silk (A. yamanmai, but rare and exp...
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Tensan Silk-Inspired Hierarchical Fibers for Smart Textile Applications
ACS Nano, 2018Co-Authors: Wenwen Zhang, Yimin Fan, Jiajia Zhong, Yuzhao Tang, Chao Ye, Markus J. Buehler, Shengjie Ling, Ke Zheng, David L. KaplanAbstract:Tensan silk, a natural fiber produced by the Japanese oak silk moth ( Antherea yamamai, abbreviated to A. yamamai), features superior characteristics, such as compressive elasticity and chemical resistance, when compared to the more common silk produced from the Domesticated Silkworm, Bombyx mori ( B. mori). In this study, the "structure-property" relationships within A. yamamai silk are disclosed from the different structural hierarchies, confirming the outstanding toughness as dominated by the distinct mesoscale fibrillar architectures. Inspired by this hierarchical construction, we fabricated A. yamamai silk-like regenerated B. mori silk fibers (RBSFs) with mechanical properties (extensibility and modulus) comparable to natural A. yamamai silk. These RBSFs were further functionalized to form conductive RBSFs that were sensitive to force and temperature stimuli for applications in smart textiles. This study provides a blueprint in exploiting rational designs from A. yamanmai, which is rare and expensive in comparison to the common and cost-effective B. mori silk to empower enhanced material properties.
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Evaluation of the Spectral Response of Functionalized Silk Inverse Opals as Colorimetric Immunosensors
ACS Applied Materials & Interfaces, 2016Co-Authors: Kelly A. Burke, David L. Kaplan, Mark A. Brenckle, Fiorenzo G. OmenettoAbstract:Regenerated silk fibroin is a high molecular weight protein obtained by purifying the cocoons of the Domesticated Silkworm, Bombyx mori. This report exploits the aqueous processing and tunable β sheet secondary structure of regenerated silk to produce nanostructures (i.e., inverse opals) that can be used as colorimetric immunosensors. Such sensors would enable direct detection of antigens by changes in reflectance spectra induced by binding events within the nanostructure. Silk inverse opals were prepared by solution casting and annealing in a humidified atmosphere to render the silk insoluble. Next, antigen sensing capabilities were imparted to silk through a three step synthesis: coupling of avidin to silk surfaces, coupling of biotin to antibodies, and lastly antibody attachment to silk through avidin–biotin interactions. Varying the antibody enables detection of different antigens, as demonstrated using different protein antigens: antibodies, red fluorescent protein, and the beta subunit of cholera to...
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Silk Fibroin Aqueous-Based Adhesives Inspired by Mussel Adhesive Proteins.
Biomacromolecules, 2015Co-Authors: Kelly A. Burke, Dane C. Roberts, David L. KaplanAbstract:Silk fibroin from the Domesticated Silkworm Bombyx mori is a naturally occurring biopolymer with charged hydrophilic terminal regions that end-cap a hydrophobic core consisting of repeating sequences of glycine, alanine, and serine residues. Taking inspiration from mussels that produce proteins rich in l-3,4-dihydroxyphenylalanine (DOPA) to adhere to a variety of organic and inorganic surfaces, the silk fibroin was functionalized with catechol groups. Silk fibroin was selected for its high molecular weight, tunable mechanical and degradation properties, aqueous processability, and wide availability. The synthesis of catechol-functionalized silk fibroin polymers containing varying amounts of hydrophilic polyethylene glycol (PEG, 5000 g/mol) side chains was carried out to balance silk hydrophobicity with PEG hydrophilicity. The efficiency of the catechol functionalization reaction did not vary with PEG conjugation over the range studied, although tuning the amount of PEG conjugated was essential for aqueous...
Wenwen Zhang - One of the best experts on this subject based on the ideXlab platform.
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tensan silk inspired hierarchical fibers for smart textile applications
ACS Nano, 2018Co-Authors: Jiajia Zhong, Yuzhao Tang, Chao Ye, Markus J. Buehler, Shengjie Ling, Wenwen Zhang, Ke Zheng, David L. KaplanAbstract:Tensan silk, a natural fiber produced by the Japanese oak silk moth (Antherea yamamai, abbreviated to A. yamamai), features superior characteristics, such as compressive elasticity and chemical resistance, when compared to the more common silk produced from the Domesticated Silkworm, Bombyx mori (B. mori). In this study, the “structure-property” relationships within A. yamamai silk are disclosed from the different structural hierarchies, confirming the outstanding toughness as dominated by the unique mesoscale fibrillar architectures. Inspired by this hierarchical construction, we fabricated A. yamamai silk-like regenerated B. mori silk fibers (RBSFs) with mechanical properties (extensibility and modulus) comparable to natural A. yamamai silk. These RBSFs were further functionalized to form conductive RBSFs that were sensitive to force and temperature stimuli for applications in smart textiles. This study provides a blueprint in exploiting novel designs from one type of silk (A. yamanmai, but rare and exp...
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Tensan Silk-Inspired Hierarchical Fibers for Smart Textile Applications
ACS Nano, 2018Co-Authors: Wenwen Zhang, Yimin Fan, Jiajia Zhong, Yuzhao Tang, Chao Ye, Markus J. Buehler, Shengjie Ling, Ke Zheng, David L. KaplanAbstract:Tensan silk, a natural fiber produced by the Japanese oak silk moth ( Antherea yamamai, abbreviated to A. yamamai), features superior characteristics, such as compressive elasticity and chemical resistance, when compared to the more common silk produced from the Domesticated Silkworm, Bombyx mori ( B. mori). In this study, the "structure-property" relationships within A. yamamai silk are disclosed from the different structural hierarchies, confirming the outstanding toughness as dominated by the distinct mesoscale fibrillar architectures. Inspired by this hierarchical construction, we fabricated A. yamamai silk-like regenerated B. mori silk fibers (RBSFs) with mechanical properties (extensibility and modulus) comparable to natural A. yamamai silk. These RBSFs were further functionalized to form conductive RBSFs that were sensitive to force and temperature stimuli for applications in smart textiles. This study provides a blueprint in exploiting rational designs from A. yamanmai, which is rare and expensive in comparison to the common and cost-effective B. mori silk to empower enhanced material properties.
Boxiong Zhong - One of the best experts on this subject based on the ideXlab platform.
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Comparative Proteomic Analysis of Posterior Silk Glands of Wild and Domesticated Silkworms Reveals Functional Evolution during Domestication
Journal of Proteome Research, 2017Co-Authors: Jianying Li, Xiaogang Ye, Jianshe Liang, Jian-ke Li, Meiyu Wu, Dan Zhao, Zhen-dong Jiang, Boxiong ZhongAbstract:The wild Silkworm Bombyx mandarina was Domesticated to produce silk in China approximately 5000 years ago. Silk production is greatly improved in the Domesticated Silkworm B. mori, but the molecular basis of the functional evolution of silk gland remains elusive. We performed shotgun proteomics with label-free quantification analysis and identified 1012 and 822 proteins from the posterior silk glands (PSGs) of wild Silkworms on the third and fifth days of the fifth instar, respectively, with 128 of these differentially expressed. Bioinformatics analysis revealed that, with the development of the PSG, the up-regulated proteins were mainly involved in the ribosome pathway, similar to what we previously reported for B. mori. Additionally, we screened 50 proteins with differential expression between wild and Domesticated Silkworms that might be involved in domestication at the two stages. Interestingly, the up-regulated proteins in Domesticated compared to wild Silkworms were enriched in the ribosome pathway,...
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shotgun proteomic analysis of the fat body during metamorphosis of Domesticated Silkworm bombyx mori
Amino Acids, 2010Co-Authors: Huijuan Yang, Zhonghua Zhou, Ming Chen, Jianying Li, Huarong Zhang, Boxiong ZhongAbstract:Protein expression profiles in the fat bodies of larval, pupal, and moth stages of Silkworm were determined using shotgun proteomics and MS sequencing. We identified 138, 217, and 86 proteins from the larval, pupal and moth stages, respectively, of which 12 were shared by the 3 stages. There were 92, 150, and 45 specific proteins identified in the larval, pupal and moth stages, respectively, of which 17, 68, and 9 had functional annotations. Among the specific proteins identified in moth fat body, sex-specific storage-protein 1 precursor and chorion protein B8 were unique to the moth stage, indicating that the moth stage fat body is more important for adult sexual characteristics. Many ribosomal proteins (L23, L4, L5, P2, S10, S11, S15A and S3) were found in pupal fat bodies, whereas only three (L14, S20, and S7) and none were identified in larval and moth fat bodies, respectively. Twenty-three metabolic enzymes were identified in the pupal stage, while only four and two were identified in the larval and moth stages, respectively. In addition, an important protein, gloverin2, was only identified in larval fat bodies. Gene ontology (GO) analysis of the proteins specific to the three stages linked them to the cellular component, molecular function, and biological process categories. The most diverse GO functional classes were involved by the relatively less specific proteins identified in larva. GO analysis of the proteins shared among the three stages showed that the pupa and moth stages shared the most similar protein functions in the fat body.
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proteomic and bioinformatic analysis on endocrine organs of Domesticated Silkworm bombyx mori l for a comprehensive understanding of their roles and relations
Journal of Proteome Research, 2009Co-Authors: Jianying Li, Zhonghua Zhou, Huijuan Yang, Ming Chen, Jine Chen, Xiang Chen, Hossein Hosseini S Moghaddam, Boxiong ZhongAbstract:Three organs of Silkworm larva endocrine system, including brain (Br), subesophageal ganglion (SG) and prothoracic glands (PG), were studied employing shotgun LC-MS/MS combined with bioinformatic analysis to comprehensively understand their roles and relations. Totally, 3430, 2683, and 3395 proteins were identified including 1885 common and 652, 253, and 790 organ-specific ones in Br, SG, and PG, respectively. Identified common-expressed proteins indicated the existence of intrinsic complex interactions among these parts of endocrine system. Most of the reputed organs-specific proteins were identified by this approach. KEGG pathway analysis showed 162 same pathways among the 169, 164, and 171 relating Br, SG, and PG. This analysis revealed functional similarities with exceptional resemblance in their metabolism and signaling pathways of the three organs. On the other hand, 70, 57, and 114 organ-specific enzymes related pathways were detected for Br, SG, and PG confirming their functional differences. Thes...
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comparative proteomic analysis between the Domesticated Silkworm bombyx mori reared on fresh mulberry leaves and on artificial diet
Journal of Proteome Research, 2008Co-Authors: Zhonghua Zhou, Huijuan Yang, Ming Chen, Yaozhou Zhang, Keping Chen, Yong Wang, Meilan Yu, Fang Yu, Jianying Li, Boxiong ZhongAbstract:To gain an insight into the effects of different diets on growth and development of the Domesticated Silkworm at protein level, we employed comparative proteomic approach to investigate the proteomic differences of midgut, hemolymph, fat body and posterior silk gland of the Silkworms reared on fresh mulberry leaves and on artificial diet. Seventy-six differentially expressed proteins were identified by MALDI TOF/TOF MS, and among them, 41 proteins were up-regulated, and 35 proteins were down-regulated. Database searches, combined with GO analysis and KEGG pathway analysis revealed that some hemolymph proteins such as Nuecin, Gloverin-like proteins, PGRP, P50 and β-N-acetylglucosamidase were related to innate immunity of the Silkworm, and some proteins identified in Silkworm midgut including Myosin 1 light chain, Tropomyosin 1, Profilin, Serpin-2 and GSH-Px were involved in digestion and nutrition absorption. Moreover, two up-regulated enzymes in fat body of larvae reared on artificial diet were identified...
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from genome to proteome great progress in the Domesticated Silkworm bombyx mori l
Acta Biochimica et Biophysica Sinica, 2008Co-Authors: Zhonghua Zhou, Huijuan Yang, Boxiong ZhongAbstract:As the only truly Domesticated insect, the Silkworm not only has great economic value, but it also has value as a model for genetics and molecular biology research. Genomics and proteomics have recently shown vast potential to be essential tools in Domesticated Silkworm research, especially after the completion of the Bombyxmori genome sequence. This paper reviews the progress of the Domesticated Silkworm genome, particularly focusing on its genetic map, physical map and functional genome. This review also presents proteomics, the proteomic technique and its application in Silkworm research.
Shengjie Ling - One of the best experts on this subject based on the ideXlab platform.
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tensan silk inspired hierarchical fibers for smart textile applications
ACS Nano, 2018Co-Authors: Jiajia Zhong, Yuzhao Tang, Chao Ye, Markus J. Buehler, Shengjie Ling, Wenwen Zhang, Ke Zheng, David L. KaplanAbstract:Tensan silk, a natural fiber produced by the Japanese oak silk moth (Antherea yamamai, abbreviated to A. yamamai), features superior characteristics, such as compressive elasticity and chemical resistance, when compared to the more common silk produced from the Domesticated Silkworm, Bombyx mori (B. mori). In this study, the “structure-property” relationships within A. yamamai silk are disclosed from the different structural hierarchies, confirming the outstanding toughness as dominated by the unique mesoscale fibrillar architectures. Inspired by this hierarchical construction, we fabricated A. yamamai silk-like regenerated B. mori silk fibers (RBSFs) with mechanical properties (extensibility and modulus) comparable to natural A. yamamai silk. These RBSFs were further functionalized to form conductive RBSFs that were sensitive to force and temperature stimuli for applications in smart textiles. This study provides a blueprint in exploiting novel designs from one type of silk (A. yamanmai, but rare and exp...
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Tensan Silk-Inspired Hierarchical Fibers for Smart Textile Applications
ACS Nano, 2018Co-Authors: Wenwen Zhang, Yimin Fan, Jiajia Zhong, Yuzhao Tang, Chao Ye, Markus J. Buehler, Shengjie Ling, Ke Zheng, David L. KaplanAbstract:Tensan silk, a natural fiber produced by the Japanese oak silk moth ( Antherea yamamai, abbreviated to A. yamamai), features superior characteristics, such as compressive elasticity and chemical resistance, when compared to the more common silk produced from the Domesticated Silkworm, Bombyx mori ( B. mori). In this study, the "structure-property" relationships within A. yamamai silk are disclosed from the different structural hierarchies, confirming the outstanding toughness as dominated by the distinct mesoscale fibrillar architectures. Inspired by this hierarchical construction, we fabricated A. yamamai silk-like regenerated B. mori silk fibers (RBSFs) with mechanical properties (extensibility and modulus) comparable to natural A. yamamai silk. These RBSFs were further functionalized to form conductive RBSFs that were sensitive to force and temperature stimuli for applications in smart textiles. This study provides a blueprint in exploiting rational designs from A. yamanmai, which is rare and expensive in comparison to the common and cost-effective B. mori silk to empower enhanced material properties.
Chao Ye - One of the best experts on this subject based on the ideXlab platform.
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tensan silk inspired hierarchical fibers for smart textile applications
ACS Nano, 2018Co-Authors: Jiajia Zhong, Yuzhao Tang, Chao Ye, Markus J. Buehler, Shengjie Ling, Wenwen Zhang, Ke Zheng, David L. KaplanAbstract:Tensan silk, a natural fiber produced by the Japanese oak silk moth (Antherea yamamai, abbreviated to A. yamamai), features superior characteristics, such as compressive elasticity and chemical resistance, when compared to the more common silk produced from the Domesticated Silkworm, Bombyx mori (B. mori). In this study, the “structure-property” relationships within A. yamamai silk are disclosed from the different structural hierarchies, confirming the outstanding toughness as dominated by the unique mesoscale fibrillar architectures. Inspired by this hierarchical construction, we fabricated A. yamamai silk-like regenerated B. mori silk fibers (RBSFs) with mechanical properties (extensibility and modulus) comparable to natural A. yamamai silk. These RBSFs were further functionalized to form conductive RBSFs that were sensitive to force and temperature stimuli for applications in smart textiles. This study provides a blueprint in exploiting novel designs from one type of silk (A. yamanmai, but rare and exp...
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Tensan Silk-Inspired Hierarchical Fibers for Smart Textile Applications
ACS Nano, 2018Co-Authors: Wenwen Zhang, Yimin Fan, Jiajia Zhong, Yuzhao Tang, Chao Ye, Markus J. Buehler, Shengjie Ling, Ke Zheng, David L. KaplanAbstract:Tensan silk, a natural fiber produced by the Japanese oak silk moth ( Antherea yamamai, abbreviated to A. yamamai), features superior characteristics, such as compressive elasticity and chemical resistance, when compared to the more common silk produced from the Domesticated Silkworm, Bombyx mori ( B. mori). In this study, the "structure-property" relationships within A. yamamai silk are disclosed from the different structural hierarchies, confirming the outstanding toughness as dominated by the distinct mesoscale fibrillar architectures. Inspired by this hierarchical construction, we fabricated A. yamamai silk-like regenerated B. mori silk fibers (RBSFs) with mechanical properties (extensibility and modulus) comparable to natural A. yamamai silk. These RBSFs were further functionalized to form conductive RBSFs that were sensitive to force and temperature stimuli for applications in smart textiles. This study provides a blueprint in exploiting rational designs from A. yamanmai, which is rare and expensive in comparison to the common and cost-effective B. mori silk to empower enhanced material properties.