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Shailesh Tripathi - One of the best experts on this subject based on the ideXlab platform.

  • The kinetic Dose Limit in room‐temperature time‐resolved macromolecular crystallography
    Journal of Synchrotron Radiation, 2012
    Co-Authors: Marius Schmidt, Vukica Šrajer, Namrta Purwar, Shailesh Tripathi
    Abstract:

    Protein X-ray structures are determined with ionizing radiation that damages the protein at high X-ray Doses. As a result, diffraction patterns deteriorate with the increased absorbed Dose. Several strategies such as sample freezing or scavenging of X-ray-generated free radicals are currently employed to minimize this damage. However, little is known about how the absorbed X-ray Dose affects time-resolved Laue data collected at physiological temperatures where the protein is fully functional in the crystal, and how the kinetic analysis of such data depends on the absorbed Dose. Here, direct evidence for the impact of radiation damage on the function of a protein is presented using time-resolved macromolecular crystallography. The effect of radiation damage on the kinetic analysis of time-resolved X-ray data is also explored.

  • The kinetic Dose Limit in room-temperature time-resolved macromolecular crystallography.
    Journal of synchrotron radiation, 2012
    Co-Authors: Marius Schmidt, Vukica Šrajer, Namrta Purwar, Shailesh Tripathi
    Abstract:

    Protein X-ray structures are determined with ionizing radiation that damages the protein at high X-ray Doses. As a result, diffraction patterns deteriorate with the increased absorbed Dose. Several strategies such as sample freezing or scavenging of X-ray-generated free radicals are currently employed to minimize this damage. However, little is known about how the absorbed X-ray Dose affects time-resolved Laue data collected at physiological temperatures where the protein is fully functional in the crystal, and how the kinetic analysis of such data depends on the absorbed Dose. Here, direct evidence for the impact of radiation damage on the function of a protein is presented using time-resolved macromolecular crystallography. The effect of radiation damage on the kinetic analysis of time-resolved X-ray data is also explored.

Marius Schmidt - One of the best experts on this subject based on the ideXlab platform.

  • The kinetic Dose Limit in room‐temperature time‐resolved macromolecular crystallography
    Journal of Synchrotron Radiation, 2012
    Co-Authors: Marius Schmidt, Vukica Šrajer, Namrta Purwar, Shailesh Tripathi
    Abstract:

    Protein X-ray structures are determined with ionizing radiation that damages the protein at high X-ray Doses. As a result, diffraction patterns deteriorate with the increased absorbed Dose. Several strategies such as sample freezing or scavenging of X-ray-generated free radicals are currently employed to minimize this damage. However, little is known about how the absorbed X-ray Dose affects time-resolved Laue data collected at physiological temperatures where the protein is fully functional in the crystal, and how the kinetic analysis of such data depends on the absorbed Dose. Here, direct evidence for the impact of radiation damage on the function of a protein is presented using time-resolved macromolecular crystallography. The effect of radiation damage on the kinetic analysis of time-resolved X-ray data is also explored.

  • The kinetic Dose Limit in room-temperature time-resolved macromolecular crystallography.
    Journal of synchrotron radiation, 2012
    Co-Authors: Marius Schmidt, Vukica Šrajer, Namrta Purwar, Shailesh Tripathi
    Abstract:

    Protein X-ray structures are determined with ionizing radiation that damages the protein at high X-ray Doses. As a result, diffraction patterns deteriorate with the increased absorbed Dose. Several strategies such as sample freezing or scavenging of X-ray-generated free radicals are currently employed to minimize this damage. However, little is known about how the absorbed X-ray Dose affects time-resolved Laue data collected at physiological temperatures where the protein is fully functional in the crystal, and how the kinetic analysis of such data depends on the absorbed Dose. Here, direct evidence for the impact of radiation damage on the function of a protein is presented using time-resolved macromolecular crystallography. The effect of radiation damage on the kinetic analysis of time-resolved X-ray data is also explored.

Namrta Purwar - One of the best experts on this subject based on the ideXlab platform.

  • The kinetic Dose Limit in room‐temperature time‐resolved macromolecular crystallography
    Journal of Synchrotron Radiation, 2012
    Co-Authors: Marius Schmidt, Vukica Šrajer, Namrta Purwar, Shailesh Tripathi
    Abstract:

    Protein X-ray structures are determined with ionizing radiation that damages the protein at high X-ray Doses. As a result, diffraction patterns deteriorate with the increased absorbed Dose. Several strategies such as sample freezing or scavenging of X-ray-generated free radicals are currently employed to minimize this damage. However, little is known about how the absorbed X-ray Dose affects time-resolved Laue data collected at physiological temperatures where the protein is fully functional in the crystal, and how the kinetic analysis of such data depends on the absorbed Dose. Here, direct evidence for the impact of radiation damage on the function of a protein is presented using time-resolved macromolecular crystallography. The effect of radiation damage on the kinetic analysis of time-resolved X-ray data is also explored.

  • The kinetic Dose Limit in room-temperature time-resolved macromolecular crystallography.
    Journal of synchrotron radiation, 2012
    Co-Authors: Marius Schmidt, Vukica Šrajer, Namrta Purwar, Shailesh Tripathi
    Abstract:

    Protein X-ray structures are determined with ionizing radiation that damages the protein at high X-ray Doses. As a result, diffraction patterns deteriorate with the increased absorbed Dose. Several strategies such as sample freezing or scavenging of X-ray-generated free radicals are currently employed to minimize this damage. However, little is known about how the absorbed X-ray Dose affects time-resolved Laue data collected at physiological temperatures where the protein is fully functional in the crystal, and how the kinetic analysis of such data depends on the absorbed Dose. Here, direct evidence for the impact of radiation damage on the function of a protein is presented using time-resolved macromolecular crystallography. The effect of radiation damage on the kinetic analysis of time-resolved X-ray data is also explored.

Vukica Šrajer - One of the best experts on this subject based on the ideXlab platform.

  • The kinetic Dose Limit in room‐temperature time‐resolved macromolecular crystallography
    Journal of Synchrotron Radiation, 2012
    Co-Authors: Marius Schmidt, Vukica Šrajer, Namrta Purwar, Shailesh Tripathi
    Abstract:

    Protein X-ray structures are determined with ionizing radiation that damages the protein at high X-ray Doses. As a result, diffraction patterns deteriorate with the increased absorbed Dose. Several strategies such as sample freezing or scavenging of X-ray-generated free radicals are currently employed to minimize this damage. However, little is known about how the absorbed X-ray Dose affects time-resolved Laue data collected at physiological temperatures where the protein is fully functional in the crystal, and how the kinetic analysis of such data depends on the absorbed Dose. Here, direct evidence for the impact of radiation damage on the function of a protein is presented using time-resolved macromolecular crystallography. The effect of radiation damage on the kinetic analysis of time-resolved X-ray data is also explored.

  • The kinetic Dose Limit in room-temperature time-resolved macromolecular crystallography.
    Journal of synchrotron radiation, 2012
    Co-Authors: Marius Schmidt, Vukica Šrajer, Namrta Purwar, Shailesh Tripathi
    Abstract:

    Protein X-ray structures are determined with ionizing radiation that damages the protein at high X-ray Doses. As a result, diffraction patterns deteriorate with the increased absorbed Dose. Several strategies such as sample freezing or scavenging of X-ray-generated free radicals are currently employed to minimize this damage. However, little is known about how the absorbed X-ray Dose affects time-resolved Laue data collected at physiological temperatures where the protein is fully functional in the crystal, and how the kinetic analysis of such data depends on the absorbed Dose. Here, direct evidence for the impact of radiation damage on the function of a protein is presented using time-resolved macromolecular crystallography. The effect of radiation damage on the kinetic analysis of time-resolved X-ray data is also explored.

Hanna Matikka - One of the best experts on this subject based on the ideXlab platform.

  • Operator’s eye lens Dose in computed tomography–guided interventions
    European Radiology, 2020
    Co-Authors: Siru Kaartinen, Minna Husso, Hanna Matikka
    Abstract:

    Objectives To survey (1) operator’s eye lens Doses in typical computed tomography (CT)-guided interventions, (2) correlation between Dose length product (DLP) and the operator’s Dose, and (3) different ways for estimating the eye lens Dose in clinical settings. Methods Doses of 16 radiologists in 164 CT-guided interventional procedures were prospectively measured during a 6-month time period upon radioprotective garments and descriptive statistical outcomes were calculated. The correlations between DLP and measured Doses were surveyed. Results On average, the operator’s Dose at the eye level (DEL, H_p(0.07)) was 22 μSv per procedure and the personal equivalent Dose H_p(10) at the collar level was 21 μSv per procedure. The mean DLP of a procedure was 320 mGy cm, where 54% resulted from the fluoroscopy, the mean exposure time being 18 s. Based on the results, the operator’s DEL could be estimated from DLP using the equation DEL (μSv) = 0.10 μSv/mGy cm × patient fluoro DLP (mGycm) ( p < 0.001), and the Dose at the collar level (DCL) using the equation DCL (μSv) = 0.12 μSv/mGy cm × patient fluoro DLP (mGy cm) ( p < 0.001). In addition, DEL (μSv) = 0.7 × DCL (μSv). Conclusions The eye lens Doses in CT-guided interventions are generally low even without protective equipment, and it is unlikely that the recommended annual equivalent Dose Limit of 20 mSv for the lens of the eye will be exceeded by conducting CT-guided interventions solely. Eye lens Dose can be roughly estimated based on either DLP of the procedure or Dose measured at the operator’s collar level. Key Points • Eye lens Doses in CT-guided operations are generally low. • It is unlikely that the ICRP recommendation of the yearly equivalent Dose Limit of 20 mSv will be exceeded by conducting CT-guided interventions solely. • Magnitude of eye lens Dose can be estimated based on either DLP of the procedure or Dose measured at the operator’s collar level.

  • Operator's eye lens Dose in computed tomography-guided interventions.
    European radiology, 2020
    Co-Authors: Siru Kaartinen, Minna Husso, Hanna Matikka
    Abstract:

    To survey (1) operator's eye lens Doses in typical computed tomography (CT)-guided interventions, (2) correlation between Dose length product (DLP) and the operator's Dose, and (3) different ways for estimating the eye lens Dose in clinical settings. Doses of 16 radiologists in 164 CT-guided interventional procedures were prospectively measured during a 6-month time period upon radioprotective garments and descriptive statistical outcomes were calculated. The correlations between DLP and measured Doses were surveyed. On average, the operator's Dose at the eye level (DEL, Hp(0.07)) was 22 μSv per procedure and the personal equivalent Dose Hp(10) at the collar level was 21 μSv per procedure. The mean DLP of a procedure was 320 mGy cm, where 54% resulted from the fluoroscopy, the mean exposure time being 18 s. Based on the results, the operator's DEL could be estimated from DLP using the equation DEL (μSv) = 0.10 μSv/mGy cm × patient fluoro DLP (mGycm) (p < 0.001), and the Dose at the collar level (DCL) using the equation DCL (μSv) = 0.12 μSv/mGy cm × patient fluoro DLP (mGy cm) (p < 0.001). In addition, DEL (μSv) = 0.7 × DCL (μSv). The eye lens Doses in CT-guided interventions are generally low even without protective equipment, and it is unlikely that the recommended annual equivalent Dose Limit of 20 mSv for the lens of the eye will be exceeded by conducting CT-guided interventions solely. Eye lens Dose can be roughly estimated based on either DLP of the procedure or Dose measured at the operator's collar level. • Eye lens Doses in CT-guided operations are generally low. • It is unlikely that the ICRP recommendation of the yearly equivalent Dose Limit of 20 mSv will be exceeded by conducting CT-guided interventions solely. • Magnitude of eye lens Dose can be estimated based on either DLP of the procedure or Dose measured at the operator's collar level.