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Anisur Rahman Khuda-bukhsh - One of the best experts on this subject based on the ideXlab platform.
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Anticancer potential of Conium maculatum extract against cancer cells in vitro: Drug-DNA Interaction and its ability to induce apoptosis through ROS generation.
Pharmacognosy magazine, 2014Co-Authors: Jesmin Mondal, Ashis Kumar Panigrahi, Anisur Rahman Khuda-bukhshAbstract:Conium maculatum extract is used as a traditional medicine for cervix carcinoma including homeopathy. However, no systematic work has so far been carried out to test its anti-cancer potential against cervix cancer cells in vitro. Thus, in this study, we investigated whether ethanolic extract of conium is capable of inducing cytotoxicity in different normal and cancer cell lines including an elaborate study in HeLa cells. Conium's effects on cell cycle, reactive oxygen species (ROS) accumulation, mitochondrial membrane potential (MMP) and apoptosis, if any, were analyzed through flow cytometry. Whether Conium could damage DNA and induce morphological changes were also determined microscopically. Expression of different proteins related to cell death and survival was critically studied by western blotting and ELISA methods. If Conium could interact directly with DNA was also determined by circular dichroism (CD) spectroscopy. Conium treatment reduced cell viability and colony formation at 48 h and inhibited cell proliferation, arresting cell cycle at sub-G stage. Conium treatment lead to increased generation of reactive oxygen species (ROS) at 24 h, increase in MMP depolarization, morphological changes and DNA damage in HeLa cells along with externalization of phosphatidyl serine at 48 hours. While cytochrome c release and caspase-3 activation led HeLa cells toward apoptosis, down-regulation of Akt and NFkB inhibited cellular proliferation, indicating the signaling pathway to be mediated via the mitochondria-mediated caspase-3-dependent pathway. CD-spectroscopy revealed that Conium interacted with DNA molecule. Overall results validate anti-cancer potential of Conium and provide support for its use in traditional systems of medicine.
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Anticancer potential of Conium maculatum extract against cancer cells in vitro: Drug-DNA Interaction and its ability to induce apoptosis through ROS generation.
Pharmacognosy Magazine, 2014Co-Authors: Jesmin Mondal, Ashis Kumar Panigrahi, Anisur Rahman Khuda-bukhshAbstract:Objective: Conium maculatum extract is used as a traditional medicine for cervix carcinoma including homeopathy. However, no systematic work has so far been carried out to test its anti-cancer potential against cervix cancer cells in vitro . Thus, in this study, we investigated whether ethanolic extract of conium is capable of inducing cytotoxicity in different normal and cancer cell lines including an elaborate study in HeLa cells. Materials and Methods: Conium's effects on cell cycle, reactive oxygen species (ROS) accumulation, mitochondrial membrane potential (MMP) and apoptosis, if any, were analyzed through flow cytometry. Whether Conium could damage DNA and induce morphological changes were also determined microscopically. Expression of different proteins related to cell death and survival was critically studied by western blotting and ELISA methods. If Conium could interact directly with DNA was also determined by circular dichroism (CD) spectroscopy. Results: Conium treatment reduced cell viability and colony formation at 48 h and inhibited cell proliferation, arresting cell cycle at sub-G stage. Conium treatment lead to increased generation of reactive oxygen species (ROS) at 24 h, increase in MMP depolarization, morphological changes and DNA damage in HeLa cells along with externalization of phosphatidyl serine at 48 hours. While cytochrome c release and caspase-3 activation led HeLa cells toward apoptosis, down-regulation of Akt and NFkB inhibited cellular proliferation, indicating the signaling pathway to be mediated via the mitochondria-mediated caspase-3-dependent pathway. CD-spectroscopy revealed that Conium interacted with DNA molecule. Conclusion: Overall results validate anti-cancer potential of Conium and provide support for its use in traditional systems of medicine.
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Low doses of ethanolic extract of Boldo (Peumus boldus) can ameliorate toxicity generated by cisplatin in normal liver cells of mice in vivo and in WRL-68 cells in vitro, but not in cancer cells in vivo or in vitro
Journal of integrative medicine, 2014Co-Authors: Jesmin Mondal, Kausik Bishayee, Ashis Kumar Panigrahi, Anisur Rahman Khuda-bukhshAbstract:Objective Use of cisplatin, a conventional anticancer Drug, is restricted because it generates strong hepatotoxicity by accumulating in liver. Therefore its anticancer potential can only be fully exploited if its own toxicity is considerably reduced. Towards this goal, ethanolic extract of the plant, Boldo ( Peumus boldus ), known for its antihepatotoxic effects, was used simultaneously with cisplatin, to test its ability to reduce cisplatin's cytotoxicity without affecting its anticancer potential. Methods The cytotoxicity of Boldo extract (BE) and cisplatin, administered alone and in combination, was determined in three cancer cell lines (A549, HeLa, and HepG2) and in normal liver cells (WRL-68). Drug-DNA Interaction, DNA damage, cell cycle, apoptosis, reactive oxygen species (ROS) and mitochondrial membrane potential (MMP, ΔΨ) were also studied. Hepatotoxicity and antioxidant activity levels were determined by alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and glutathione assays in mice. The cytotoxicity of related proteins was tested by Western blotting. Results Co-administration of BE and cisplatin increased viability of normal cells, but had no effect on the viability of cancer cells. Boldo protected liver from damage and normalized different antioxidant enzyme levels in vivo and also reduced ROS and re-polarized MMP in vitro. Bax and cytochrome c translocation was reduced with caspase 3 down-regulation. Further, a Drug-DNA Interaction study revealed that BE reduced cisplatin's DNA-binding capacity, resulting in a reduction in DNA damage. Conclusion Results indicated that a low dose of BE could be used beneficially in combination with cisplatin to reduce its toxicity without hampering cisplatin's anticancer effect. These findings signify a potential future use of BE in cancer therapy.
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Oleanolic acid isolated from ethanolic extract of Phytolacca decandra induces apoptosis in A375 skin melanoma cells： Drug-DNA Interaction and signaling cascade
Journal of integrative medicine, 2014Co-Authors: Samrat Ghosh, Kausik Bishayee, Anisur Rahman Khuda-bukhshAbstract:Objective Oleanolic acid (OA) has been reported to have anticancer effects, but the extent of its cytotoxicity, its ability to interact with nuclear DNA, its action against skin melanoma, as well as the molecular mechanism of its action against cell proliferation and in support of cell death are still unexplored. This led us to examine the efficacy of OA, a bioactive compound isolated from Phytolacca decandra , on these issues in the present investigation. Methods Studies related to analyses of cell viability, Drug-DNA Interaction, cell proliferation, cell cycle and epidermal growth factor receptor (EGFR) activity were performed. To investigate whether cells undergo apoptosis, studies like fluorescence microscopy, poly (ADP-ribose) polymerase (PARP) degradation, annexin V-fluorescein isothiocyanate/propidium iodide assay, alteration in mitochondrial membrane potential and activity of some relevant signaling proteins were performed. Results OA displayed a minimal and negligible cytotoxic effect on normal HaCaT cells (skin keratinocytes) and peripheral blood mononuclear cells but by contrast it reduced A375 cell viability significantly. OA interacted with nuclear DNA quickly after exposure. It acted as an antiproliferative agent. It suppressed EGFR activity. OA administration led the cells to mitochondria-dependent caspase 3-mediated apoptosis. Conclusion OA interacts with cellular DNA, inhibits proliferation possibly through modulating EGFR activity and induces mitochondria-dependent caspase 3-mediated apoptosis in A375 cells which would qualify it as a potent anticancer agent.
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Ethanolic extract of the Goldenseal, Hydrastis canadensis, has demonstrable chemopreventive effects on HeLa cells in vitro: Drug-DNA Interaction with calf thymus DNA as target.
Environmental toxicology and pharmacology, 2013Co-Authors: Santu Kumar Saha, Avinaba Mukherjee, Kakali Bhadra, Sourav Sikdar, Naoual Boujedaini, Anisur Rahman Khuda-bukhshAbstract:Abstract This study tested chemotherapeutic potential of Hydrastis canadensis (HC) extract in HeLa cells in vitro, with emphasis on its Drug–DNA Interaction and apoptosis induction ability. Nuclear uptake of HC by DAPI, Ao/Eb staining and internucleosomal DNA damage by comet assay was studied through fluorescence microscopy. Possible changes in MMP and apoptotic signalling events were critically analyzed. Cell cycle progression studied through FACS and fragmented DNA through “TUNEL” assay were critically analyzed. RT-PCR studies were conducted for analyzing Cyt-C and Bax translocation in mitochondrial and cytosolic extracts, and Caspase 3 in whole cell lysate. Role of p53-mediated regulation of NF-κβ and TNF-α was elucidated by Western blot analysis. Data of CD and Tm profile of CT-DNA were analyzed. Overall results indicated anti-cancer potential of HC through its ability to induce apoptosis, and Interaction with CT-DNA that changed structural conformation of DNA, proving HC to be a promising candidate for chemoprevention.
V D Gupta - One of the best experts on this subject based on the ideXlab platform.
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Drug DNA Interaction a theoretical study on the binding of thionine with DNAs of varying base composition
Current Applied Physics, 2013Co-Authors: Ghazala Yunus, Seema Srivastava, Mohammed Kuddus, V D GuptaAbstract:Abstract The recent studies carried out on the binding of small molecule to deoxyribonucleic acids suggested that the intercalation of a tricyclic heteroaromatic molecule, thionine, with natural DNAs provided thermal stabilization to the DNA complex. In the present study, we reported theoretical analysis of thionine binding with natural DNAs of varying base composition by using an amended Zimm and Bragg theory, to explain the melting behaviour and heat capacity of DNAs with and without thionine binding. We used experimental models of Paul et al. for implementing this study (Paul et al., 2010). The sharpness of transition has been examined in terms of half width and sensitivity parameter (Δ H / σ ). The results of theoretical analysis concluded that the various parameters such as heat capacity curve, transition profile, half widths and sharpness of the transition are in good agreement with the experimental measurements for binding of thionine determined through DSC. The theoretical analysis proposed in this study, therefore, may be useful to understand Interaction of small molecules with deoxyribonucleic acids. This approach may also be applied to design DNA binding therapeutic molecules and in the process of Drug formulation and development.
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Drug–DNA Interaction: A theoretical study on the binding of thionine with DNAs of varying base composition
Current Applied Physics, 2013Co-Authors: Ghazala Yunus, Seema Srivastava, Mohammed Kuddus, V D GuptaAbstract:Abstract The recent studies carried out on the binding of small molecule to deoxyribonucleic acids suggested that the intercalation of a tricyclic heteroaromatic molecule, thionine, with natural DNAs provided thermal stabilization to the DNA complex. In the present study, we reported theoretical analysis of thionine binding with natural DNAs of varying base composition by using an amended Zimm and Bragg theory, to explain the melting behaviour and heat capacity of DNAs with and without thionine binding. We used experimental models of Paul et al. for implementing this study (Paul et al., 2010). The sharpness of transition has been examined in terms of half width and sensitivity parameter (Δ H / σ ). The results of theoretical analysis concluded that the various parameters such as heat capacity curve, transition profile, half widths and sharpness of the transition are in good agreement with the experimental measurements for binding of thionine determined through DSC. The theoretical analysis proposed in this study, therefore, may be useful to understand Interaction of small molecules with deoxyribonucleic acids. This approach may also be applied to design DNA binding therapeutic molecules and in the process of Drug formulation and development.
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Drug-DNA Interaction: A Theoretical Study of the Stability of CP-DNA Binding with Thionine
Open Journal of Applied Sciences, 2012Co-Authors: Ghazala Yunus, Seema Srivastava, V D GuptaAbstract:The recent study on binding of small molecules to double stranded DNA suggested that the intercalation of a tricyclic heteroaromatic molecule, thionine, with natural DNA provided thermal stabilization to the complex. In the present study, we reported theoretical analysis of thionine binding with Clostridium perfringenes DNA duplex (CP-DNA) by using an amended Zimm and Bragg theory, to explain the melting behaviour and heat capacity of CP-DNA with and without thionine binding. The experimental models of Paul et al. (2010) have been used for the study. The sharpness of transition has been examined in terms of half width and sensitivity parameter (?H/σ). The results of theoretical approach suggested that the various parameters such as transition profile, sharpness of the transition, heat capacity curve and half widths are in good agreement with the experimental measurements for binding of thionine. Therefore, the proposed theoretical analysis may be useful in order to understand Interaction of small molecules to DNA that may be applied in the process of Drug development and for designing more potential DNA binding therapeutic molecules.
Erkang Wang - One of the best experts on this subject based on the ideXlab platform.
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oligonucleotide stabilized silver nanoclusters as fluorescence probe for Drug DNA Interaction investigation
Analytica Chimica Acta, 2011Co-Authors: Jipei Yuan, Weiwei Guo, Erkang WangAbstract:In this work, oligonucleotide stabilized silver nanoclusters as novel fluorescent probes were successfully utilized for the Drug-DNA Interaction study. Silver nanoclusters were proved to be sensitive probes for the Drugs investigated (including of two kinds of intercalators, daunorubicin and quinacrine, as well as a non-intercalating binder bisBenzimide H 33258), as the detection limits at 10(-8) mol L(-1) level of studied Drugs can be achieved. The Interactions of Drugs and calf thymus DNA were investigated using non-linear fit analysis, and the binding constants as well as binding site sizes were obtained. As biocompatible materials, silver nanoclusters are promising in the chemical especially biochemical analysis fields.
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Oligonucleotide stabilized silver nanoclusters as fluorescence probe for Drug–DNA Interaction investigation
Analytica chimica acta, 2011Co-Authors: Jipei Yuan, Weiwei Guo, Erkang WangAbstract:In this work, oligonucleotide stabilized silver nanoclusters as novel fluorescent probes were successfully utilized for the Drug-DNA Interaction study. Silver nanoclusters were proved to be sensitive probes for the Drugs investigated (including of two kinds of intercalators, daunorubicin and quinacrine, as well as a non-intercalating binder bisBenzimide H 33258), as the detection limits at 10(-8) mol L(-1) level of studied Drugs can be achieved. The Interactions of Drugs and calf thymus DNA were investigated using non-linear fit analysis, and the binding constants as well as binding site sizes were obtained. As biocompatible materials, silver nanoclusters are promising in the chemical especially biochemical analysis fields.
Anisur Rahman Khudabukhsh - One of the best experts on this subject based on the ideXlab platform.
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nano encapsulated chlorophyllin significantly delays progression of lung cancer both in in vitro and in vivo models through activation of mitochondrial signaling cascades and Drug DNA Interaction
Environmental Toxicology and Pharmacology, 2016Co-Authors: Jayeeta Das, Jesmin Mondal, Asmita Samadder, Suresh K Abraham, Anisur Rahman KhudabukhshAbstract:Chlorophyllin (CHL), a sodium-copper-salt derived from chlorophyll, has been widely used as a food-dye, also reportedly having some anti-cancer effect. We tested if PLGA-loaded CHL (NCHL) could have additional protective abilities through its faster and targeted Drug delivery in cancer cells. Physico-chemical characterization of NCHL was done through atomic-force microscopy and UV-spectroscopy. NCHL demonstrated greater ability of Drug uptake and strong anti-cancer potentials in non-small cell lung cancer cells, A549, as revealed from data of% cell viability, generation of reactive-oxygen-species and expression of bax, bcl2, caspase3, p53 and cytochrome c proteins. Circular dichroic spectral data indicated strong binding of NCHL with calf-thymus-DNA, causing a conformational/structural change in DNA. Further, NCHL could cross the blood-brain-barrier in mice and showed greater efficacy in recovery process of tissue damage, reduction in chromosomal aberrations and% of micronuclei in co-mutagens (Sodiumarsenite+Benzo[a]Pyrene)-treated mice at a much reduced dose, indicating its use in therapeutic oncology.
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diarylheptanoid myricanone isolated from ethanolic extract of myrica cerifera shows anticancer effects on hela and pc3 cell lines signalling pathway and Drug DNA Interaction
Journal of Integrative Medicine, 2013Co-Authors: Avijit Paul, Kausik Bishayee, Ratan Sadhukhan, Asmita Samadder, Sreemanti Das, Jayeeta Das, Anisur Rahman KhudabukhshAbstract:Objective To test if myricanone (C 21 H 24 O 5 ), a cyclic diarylheptanoid, has anticancer effects on two different cancer cell lines HeLa and PC3. The present study was conducted with a note on the Drug-DNA Interaction and apoptotic signalling pathway. Methods Several studies like cytotoxicity, nuclear damage, annexin-V-fluorescein isothiocyanate (FITC)/propidium iodide (PI)-labelled apoptotic assay and cell cycle arrest, immunoblot and reverse transcriptase-polymerase chain reaction (RT-PCR) were used following standard protocols. Circular dichroism (CD) spectroscopy was also done to evaluate whether myricanone effectively interacted with DNA to bring about conformational changes that could strongly inhibit the cancer cell proliferation. Results Myricanone showed a greater cytotoxic effect on PC3 cells than on HeLa cells. Myricanone promoted G 0 /G 1 arrest in HeLa cells and S phase arrest in PC3 cells. Nuclear condensation and annexin V-FITC/PI studies revealed that myricanone promoted apoptotic cell death. CD spectroscopic data indicated that myricanone had an Interaction with calf thymus DNA that changed DNA structural conformation. RT-PCR and immunoblot studies revealed that myricanone activated the apoptotic signalling cascades through down-regulation of transcription factors like nuclear factor-κB (NF-κB) (p65), and signal transducers and activators of transcription 3 (STAT3); cell cycle regulators like cyclin D1, and survivin and other signal proteins like Bcl-2 and up-regulation of Bax, caspase-9 and caspase-3. Conclusion Myricanone induced apoptosis in both types of cancer cells by triggering caspase activation, and suppression of cell proliferation by down-regulation of NF-κB and STAT3 signalling cascades, which makes it a suitable candidate for possible use in the formulation of therapeutic agent for combating cancer.
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quercetin induces cytochrome c release and ros accumulation to promote apoptosis and arrest the cell cycle in g2 m in cervical carcinoma signal cascade and Drug DNA Interaction
Cell Proliferation, 2013Co-Authors: Kausik Bishayee, Avinaba Mukherjee, Ratan Sadhukhan, Jesmin Mondal, Samrat Ghosh, Anisur Rahman KhudabukhshAbstract:Objectives Small aromatic compounds like flavonoids can intercalate with DNA molecules bringing about conformational changes leading to reduced replication and transcription. Here, we have examined one dietary flavonoid, quercetin (found in many fruit and vegetables), for possible anti-cancer effects, on HeLa cells originally derived from a case of human cervical cancer. Material and methods By circular dichroism spectroscopy we tested whether quercetin effectively interacted with DNA to bring about conformational changes that would strongly inhibit proliferation and migration of the HeLa cells. Cytotoxic effects of quercetin on cancer/normal cells, if any, were determined by MTT assay and such depolarization of mitochondrial membrane potential, as a consequence of quercetin treatment, and accumulation of reactive oxygen species (ROS) also were studied, by FACS analysis and expression profiles of different anti- and pro-apoptotic genes and their products were determined. Results Quercetin intercalated with calf thymus cell DNA and HeLa cell DNA and inhibition of anti-apoptotic AKT and Bcl-2 expression were observed. Levels of mitochondrial cytochrome-c were elevated and depolarization of mitochondrial membrane potential occurred with increase of ROS; upregulation of expression of p53 and caspase-3 activity were also noted. These alterations in signalling proteins and externalization of phosphotidyl serine residues were involved with initiation of apoptosis. Reduced AKT expression suggested reduction in cell proliferation and metastasis potential, with arrest of the cell cycle at G2/M. Conclusion Quercetin would have potential for use in cervical cancer chemotherapy.
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6 gingerol induces caspase 3 dependent apoptosis and autophagy in cancer cells Drug DNA Interaction and expression of certain signal genes in hela cells
European Journal of Pharmacology, 2012Co-Authors: Debrup Chakraborty, Kausik Bishayee, Samrat Ghosh, Raktim Biswas, Sushil Kumar Mandal, Anisur Rahman KhudabukhshAbstract:Abstract [6]-Gingerol, a pharmacologically important bioactive component of ginger, has been reported to have anti-hyperglycemic, anti-cancer and anti-oxidative properties, but mechanisms through which these are achieved are largely unclear. The present study focuses on apoptosis and autophagy, two key events of anti-cancer activity, in HeLa cells treated with [6]-gingerol. The treated cells showed several morphological changes, including externalization of phosphatidyl serine, degradation of DNA and increase in TUNEL positivity. Furthermore, there was depolarization of mitochondrial membrane potential, providing evidence of mitochondria mediated apoptosis. The expression of caspase 3 and PARP was increased in cells exposed to [6]-gingerol. Circular dichroism study for testing Drug–DNA Interaction with both calf thymus and nuclear DNA as target revealed that the Drug had potential to bind with the nuclear DNA and induce conformational changes of DNA. The over-expression of NFkβ, AKT and Bcl2 genes in cancer cells was down-regulated by [6]-gingerol treatment. On the other hand the expression levels of TNFα, Bax and cytochrome c were enhanced in [6]-gingerol treated cells. Thus, overall results suggest that [6]-gingerol has potential to bind with DNA and induce cell death by autophagy and caspase 3 mediated apoptosis.
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possible signaling cascades involved in attenuation of alloxan induced oxidative stress and hyperglycemia in mice by ethanolic extract of syzygium jambolanum Drug DNA Interaction with calf thymus DNA as target
European Journal of Pharmaceutical Sciences, 2011Co-Authors: Asmita Samadder, Debrup Chakraborty, Arnab Kumar De, Soumya Sundar Bhattacharyya, Kakali Bhadra, Anisur Rahman KhudabukhshAbstract:Abstract We injected alloxan (100 mg/kg b.w.) in mice ( Mus musculus ) intra-peritoneally to induce hyperglycemia and divided the hyperglycemic mice into two sub-groups: one was fed ethanolic extract of Syzygium jambolanum (EESJ) (20 mg/kg b.w. for 8 weeks) and the other 85% ethyl alcohol (“vehicle”-control). Chromatographic and mass spectroscopic studies of EESJ revealed two principal components, one corresponding to an iridoid glycoside. We estimated blood glucose, glycosylated hemoglobin, glucokinase, and fructosamine and analyzed the expression of marker proteins like insulin, GLUT2, and GLUT4. We also studied anti-oxidant biomarkers like lipid peroxidase, superoxide dismutase, total thiole and catalase. We assayed generation of reactive oxygen species (ROS) and several inflammatory and apoptotic signal proteins like NFkB, IFNγ, iNOS, Bcl 2, Bax, STAT1 and Caspase3. We further evaluated the effects of hyperglycemia on DNA through comet assay and DNA fragmentation study and assessed Drug-DNA Interaction by comparative analysis of circular dichroism (CD) spectral data and melting temperature profiles ( T m ) of calf thymus DNA treated with or without EESJ. We observed an elevation of all biomarkers for oxidative stress, generation of ROS and activation of NFkB and down regulation in expression of insulin, GLUT2 and glucokinase in hyperglycemic mice. Administration of EESJ reversed these changes. Histo-pathological observations of pancreas, liver and kidney also revealed relevant changes. Data of CD and ( T m ) indicated an Interaction of EESJ with calf thymus DNA, indicating change in structure and conformation. Thus, EESJ has anti-oxidant as well as anti-hyperglycemic activities in diabetic mice, and potentially useful in management of hyperglycemia.
Arzum Erdem - One of the best experts on this subject based on the ideXlab platform.
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Carbon quantum dot modified electrodes developed for electrochemical monitoring of Daunorubicin-DNA Interaction
Journal of Electroanalytical Chemistry, 2020Co-Authors: Ece Eksin, Huseyin Senturk, Erhan Zor, Haluk Bingol, Arzum ErdemAbstract:Abstract The carbon quantum dot (cQD) modified disposable pencil graphite electrodes were developed for the first time in this study for electrochemical monitoring of Drug-DNA Interaction. The biomolecular Interaction between calf thymus double stranded DNA (ctDNA) and an anthracycline antineoplastic Drug, Daunorubicin (DNR) was investigated by differential pulse voltammetry (DPV) technique. For this purpose, experimental conditions, such as, the concentration of ctDNA, concentration of DNR and Interaction time were optimized. Under optimum conditions, the detection limits of DNR and ctDNA were found to be 0.02 μg/mL and 0.89 μg/mL, respectively. The effect of Interaction time between DNR and ctDNA was explored upon to the changes at both DNR and guanine oxidation signals. Drug-DNA Interaction process was also examined by electrochemical impedance spectroscopy (EIS) technique.
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Chitosan modified graphite electrodes developed for electrochemical monitoring of Interaction between daunorubicin and DNA
Sensing and bio-sensing research, 2019Co-Authors: Gulsah Congur, Ece Eksin, Arzum ErdemAbstract:Abstract Chitosan (CHIT) modified single-use electrodes were developed for the first time in the present study and applied for electrochemical monitoring of anticancer Drug-DNA Interaction. Under this aim, pencil graphite electrode (PGE) was used as the biosensor platform and the modification of PGEs using biopolymer, CHIT was performed by passive adsorption process. Microscopic and electrochemical characterizations of CHIT modified PGEs were performed. An anticancer Drug, Daunorubicin (DNR) was analyzed by using both PGEs and CHIT-PGEs. Moreover, the effect of CHIT modification on biosensor development upon to the sensitivity of voltammetric detection of DNR and DNA was also investigated. The Interaction between DNA and DNR was then performed and accordingly, the voltammetric measurements were performed before/after Interaction process by using differential pulse voltammetry (DPV) technique.
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gold nanoparticle polymer nanocomposite for highly sensitive Drug DNA Interaction
Analyst, 2015Co-Authors: Filiz Kuralay, Arzum ErdemAbstract:The Interaction of the anticancer Drug mitomycin C (MC) and DNA immobilized on gold a nanoparticle/polyvinylferrocenium (AuNP/PVF+) coated electrode is presented. This is the first attempt to prepare a biocompatible nanoparticle/redox polymer composite in a one-step and easy electropolymerization procedure and then use the coated electrode for MC–DNA Interaction. The prepared electrode exhibits high sensitivity for the investigation of Drug–DNA Interaction.
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Gold nanoparticle/polymer nanocomposite for highly sensitive Drug–DNA Interaction
The Analyst, 2015Co-Authors: Filiz Kuralay, Arzum ErdemAbstract:The Interaction of the anticancer Drug mitomycin C (MC) and DNA immobilized on gold a nanoparticle/polyvinylferrocenium (AuNP/PVF+) coated electrode is presented. This is the first attempt to prepare a biocompatible nanoparticle/redox polymer composite in a one-step and easy electropolymerization procedure and then use the coated electrode for MC–DNA Interaction. The prepared electrode exhibits high sensitivity for the investigation of Drug–DNA Interaction.
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Chitosan/Ionic Liquid Composite Electrode for Electrochemical Monitoring of the Surface-Confined Interaction Between Mitomycin C and DNA
Electroanalysis, 2013Co-Authors: Ece Eksin, Mihrican Muti, Arzum ErdemAbstract:In the present study a chitosan/ionic liquid modified pencil graphite electrode (CHIT-IL-PGEs) was developed for the first time for enhanced electrochemical monitoring of nucleic acid, and the Interaction of the anticancer Drug Mitomycin C (MC) and calf thymus double stranded DNA (dsDNA) by measuring the oxidation signals of MC and guanine in the same voltammetric scale. Differential pulse voltammetry, cyclic voltammetry and electrochemical impedance spectroscopy techniques were used to evaluate the performance of the CHIT-IL based biosensor on electrochemical monitoring of DNA, and Drug-DNA Interaction. The experimental parameters, IL, dsDNA and MC concentration and the Interaction time were then optimized.
