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Dabing Zhang - One of the best experts on this subject based on the ideXlab platform.
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molecular characterization of a Duck Hepatitis Virus 3 like astroVirus
Veterinary Microbiology, 2014Co-Authors: Ning Liu, Fumin Wang, Jiajian Shi, Lisha Zheng, Xiaoyan Wang, Dabing ZhangAbstract:Using an ORF1b-based astroVirus-specific reverse transcription (RT)-PCR assay, a Duck Hepatitis Virus type 3 (DHV-3)-like astroVirus was detected from four intestinal samples collected from diseased Ducks in China. Complete genome sequencing and comparative sequence analysis showed that the four Duck astroVirus (DAstV) isolates were closely related and possessed a typical astroVirus genome organization. Genetic analysis of the complete ORF2 region revealed that mean amino acid genetic distances between the DHV-3-like isolates and previously known avastroVirus species were between 0.579 and 0.721, suggesting that the DHV-3-like isolates could be classified as an additional avastroVirus species. In the ORF1a and ORF1b regions, however, mean amino acid genetic distances between the DHV-3-like Viruses and the turkey astroVirus 2 (TAstV-2)-like isolates were substantially less than those between TAstV-2-like isolates and DAstV/C-NGB-like astroViruses belonging to the same species. Pairwise comparisons and phylogenetic analyses demonstrated that the DHV-3-like isolates were most closely related to TAstV-2-like Viruses in ORF1a and ORF1b, while showed highest similarity with the chicken astroVirus (CAstV) 612-like Viruses in ORF2. These findings provide evidence that recombination events may have occurred during evolution of the avastroViruses and support the view that genomic analysis is required for classification of the avastroViruses.
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Molecular analysis of Duck Hepatitis Virus type 1.
Virology, 2007Co-Authors: Chunyu Ding, Dabing ZhangAbstract:Abstract The genome sequence of a Duck Hepatitis Virus type 1 (DHV-1) strain was determined. Comparative sequence analysis showed that the genome possesses a typical picornarivus organization and also exhibits several unique features, such as the similarity of internal ribosome entry site to that of Porcine teschoVirus 1 and Hepatitis C Virus , the presence of a longest 3′ untranslated region and a shorter leader protein in the Picornaviridae , the absence of a predicted maturation cleavage of VP0, the association of an aphthoVirus-like 2A1 and parechoVirus-like 2A2, and the unprecedented presence of an AIG1 domain in the N-terminus of 2A2. It is concluded that DHV-1 belongs to a new group of the family Picornaviridae that may form a separate genus most closely related to the genus ParechoVirus .
Zhang Gui-hong - One of the best experts on this subject based on the ideXlab platform.
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Isolation and Identification Analysis of Duck Hepatitis Virus GD Strain by RT-PCR
China Animal Husbandry & Veterinary Medicine, 2012Co-Authors: Zhang Gui-hongAbstract:In order to complete a Duck Hepatitis Virus(DHV) stain's isolation and sero-genotyping research,the suspected DHV liver from died Duckling in Guangdong province was selected as sample,and then was carried on regression analysis,ELD50 determination and Duck embryo neutralization test after viral isolation,Duck embryo inoculation and purification.The results showed that the serotype of the Duck Hepatitis Virus was typeⅠ.According to RT-PCR detect and sequence analysis,it was also identified as genetype Ⅰ of DHV.Named the isolated Virus strain as GD.
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Determination and analysis of the complete genomic sequence of Duck Hepatitis Virus GD strain
Heilongjiang Animal Science and Veterinary Medicine, 2012Co-Authors: Zhang Gui-hongAbstract:To lay the foundation for the pathogenesis of Duck Hepatitis Virus at the molecular level,further enriching the information of genetic variation and evolution for DHV,the complete sequence of Duck Hepatitis Virus type 1(DHV-1) GD strain was amplified by RT-PCR.After being sequenced,the phylogenetic tree was analyzed.The results showed that the full genomic length of GD consisted of 7 690 nt without Poly(A) tail,and had 5′-and 3′-terminal non-coding regions of 626 and 314 nucleotides respectively,including a single open reading frame(ORF)627~7 376 nt,which encoded a polypeptide of 2 249 aminoacids.The comparative genome analysis with other reference strains of DHV-1 indicated that the nucleotide sequence homology was 94.8% to 99.7% and the amino acid sequence homology was 97.7% to 99.6%.Compared to the new DHV(DHV-N) strains isolated in Taiwan Province of China and South Korea,the homologies of the nucleotide sequence and amino acid sequence were only 72.7% to 73.2% and 81.8% to 83.4% respectively.The phylogenetic analysis indicated that the GD strain had a closer genetic relationship with the reference strains of DHV-1 located at the same branch of the tree,farther than the DHV-N strains located at the different branches of the tree.
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Whole-genome analysis of one strain of Duck Hepatitis Virus type I
Heilongjiang Animal Science and Veterinary Medicine, 2011Co-Authors: Zhang Gui-hongAbstract:To investigate the variation and genetic evolution of Duck Hepatitis Virus type Ⅰ,the genome sequence of Duck Hepatitis Virus which isolated from foshan was analyzed.The results show that the full length of genome was 7 690 bp,including one single open reading frame(ORF) encoding a polypeptide of 2 249 amino acids(aa).Compared to the other genome of DHV-Ⅰ from GenBank,VP1 was the largest variation.The hypervariable regions were from 180 to 220 aa.The amino acid sequences shared 97.5% to 99.2% amino acid identity with those of classical DHV-Ⅰ.The whole nucleotide sequence shared 94.0%-97.4% identity with those genomes.The isolate was situated in the DHV-Ⅰ branch of the phylogenetic tree and was less related with Taiwan strains and Korean strains which were in different branches.
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Construction of tandem expression vector VP1 gene for Duck Hepatitis Virus(DHV) type 1
Heilongjiang Animal Science and Veterinary Medicine, 2011Co-Authors: Zhang Gui-hongAbstract:To construct a recombinant expressing plasmid including two VP1 genes(2VP1) of DHV,according to complete genome of Duck Hepatitis Virus 1(DHV-1),two special primer pair were designed to amplify VP1genes(2VP1) of DHV.The gene of VP1was cloned into the vector T,then it was subcloned into the prokaryotic expression vector pET-30a(+)after the sequence was identified by BamHⅠ,XhoⅡ,BgⅢ digestion and sequencing analysis.The results showed that the ligation direction of the objective gene fragment was correct.It indicates that the prokaryotic expression vector pET-30a-2VP1 has been constructed successfully.
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Development of identify RT-PCR method for Duck Hepatitis Virus type I(DHV-I) and new serotype Duck Hepatitis Virus
Heilongjiang Animal Science and Veterinary Medicine, 2009Co-Authors: Zhang Gui-hongAbstract:In the study,identify RT-PCR method for Duck Hepatitis Virus type Ⅰ(DHV-Ⅰ) and new serotype Duck Hepatitis Virus was developed successfully using two pairs of primers designed with Primer Primier 5.0 software according to the complete genome sequence of DHV-Ⅰ and new serotype Duck Hepatitis Virus deposited in GenBank(GenBank accession numer are EU841005 and EF585200,respectively)and ZJ strain and R strain in our lab.The result indicated that this method was shown to specifically amplify a 471 bp fragment from DHV-Ⅰ or a 705 bp fragment from new serotype Duck Hepatitis Virus FS strain.Test on clinical samples showed that this method was highly specific and can detect DHV-Ⅰ to 0.8 ng/μL and new serotype Duck Hepatitis Virus to 1 ng/μL in template respectively.Therefore,the identify RT-PCR could be used as an effective tool for diagnosis of DHV-Ⅰ and new serotype Duck Hepatitis Virus in clinical samples,and molecule epidemiological investigations.
Shen Zhiqiang - One of the best experts on this subject based on the ideXlab platform.
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Establishment and application of a nest RT-PCR method for detection of Duck Hepatitis Virus new type
Chinese journal of veterinary science, 2013Co-Authors: Shen ZhiqiangAbstract:Based on N-DHV gene sequence in GenBank,synthesized outer and inner two pairs of primers,established a nested PCR for N-DHV detection.The method was used for detection of Duck Hepatitis Virus type Ⅰ(DHV-Ⅰ),Newcastle disease Virus(NDV),infection bursal disease Virus(IBDV),Duck enteritis Virus(DEV),Duck parvoVirus(DPV),all of the PCR results were negative;the method sensitivity first amplification is 10 pg,the second amplification sensitivity is 0.1 pg,100 times higher than the first amplification.This method had good specificity,sensitivity and reproducibility,could be used for rapid diagnosis of N-DHV disease.
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Isolation and identification of new serotype Duck Hepatitis Virus and Pasteurella multocida from 10-day-old meat Ducklings
Chinese journal of veterinary science, 2012Co-Authors: Shen ZhiqiangAbstract:This experiment was conducted to identify the pathogen of 10-day-old meat Ducklings which was suspected of infecting Duck Hepatitis Virus and Pasteurella multocida(P.mutocida) simultaneously.The conventional test of Virus and bacterium,RT-PCR and PCR methods were all used to identify the viral isolate and bacterial isolate respectively.Results indicated that the isolated Virus is new serotype Duck Hepatitis Virus,the isolated bacterium is P.multocida subsp.mutocida serotype A.In animal pathogenicity test,P.multocida isolate is similar in virulent to national standard velogen strain C48-1,the result of pathogenicity test on 10-day-old meat Ducklings demonstrated that Pasteurella multocida is lethiferous to 10-day-old Ducklings in certain CFUs.The meat Ducklings were infected by new serotype Duck Hepatitis Virus and Pasteurella multocida subsp.mutocida serotype A simultaneously.This is the first report that Pasteurella multocida was isolated from 10-day-old Ducklings which affected new serotype Duck Hepatitis Virus in China.
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Establishment and application of a nested RT-PCR method for detection of Duck Hepatitis Virus typeI
Chinese Veterinary Science, 2011Co-Authors: Shen ZhiqiangAbstract:Based on the genome sequence of Duck Hepatitis Virus typeⅠ(DHV-Ⅰ) in GenBank,two pairs of specific primers were designed using Oligo6.0 software.A nested RT-PCR method for detection of DHV-Ⅰ was established.This method had good specificity,sensitivity and reproducibility,and might detect low content(1 pg) DHV-Ⅰ accurately and rapidly.This method will solve the difficulties of the disease detection in tissues,and could be used for rapid diagnosis and helpful for further study and elimination of Duck viral Hepatitis typeⅠ.
Hsiang-jung Tsai - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of a new serotype of Duck Hepatitis Virus.
Virus research, 2007Co-Authors: Chun-hsien Tseng, Hsiang-jung TsaiAbstract:Duck Hepatitis strains 90D and 04G were determined to be antigenically unrelated to type 1 Duck Hepatitis Virus (DHV-1) by in vitro cross-neutralization assay. The genome sequences of 90D and 04G revealed that both strains of the new serotype DHV (N-DHV) possessed a typical picornaVirus genome organization apart from the unique possession of three in-tandem 2A genes present in DHV-1. The 2A1, 2A2, and 2A3 proteins represented an aphthoVirus-like 2A protein, AIG1-like protein, and human parechoVirus-like 2A protein, respectively. The N-DHV genome displayed unique features, compared to the DHV-1 genome. The 366 nt 3'UTR of N-DHV, the largest determined thus far among picornaViruses, was 52 nt longer than DHV-1. The pairwise percent identity of the nucleic acid and amino acid sequences at 1D region of N-DHV and DHV-1 were only 69.1-69.7 and 70.1-70.5%, respectively. Finally, phylogenetic and evolutionary analysis of N-DHV revealed that the N-DHV and DHV-1 belong to two different clusters of a novel genus in the Picornaviridae family.
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Molecular characterization of a new serotype of Duck Hepatitis Virus
Virus Research, 2007Co-Authors: Chun-hsien Tseng, Hsiang-jung TsaiAbstract:Duck Hepatitis strains 90D and 04G were determined to be antigenically unrelated to type 1 Duck Hepatitis Virus (DHV-1) by in vitro crossneutralization assay. The genome sequences of 90D and 04G revealed that both strains of the new serotype DHV (N-DHV) possessed a typical picornaVirus genome organization apart from the unique possession of three in-tandem 2A genes present in DHV-1. The 2A1, 2A2, and 2A3 proteins represented an aphthoVirus-like 2A protein, AIG1-like protein, and human parechoVirus-like 2A protein, respectively. The N-DHV genome displayed unique features, compared to the DHV-1 genome. The 366 nt 3 � UTR of N-DHV, the largest determined thus far among picornaViruses, was 52 nt longer than DHV-1. The pairwise percent identity of the nucleic acid and amino acid sequences at 1D region of N-DHV and DHV-1 were only 69.1–69.7 and 70.1–70.5%, respectively. Finally, phylogenetic and evolutionary analysis of N-DHV revealed that the N-DHV and DHV-1 belong to two different clusters of a novel genus in the Picornaviridae family. © 2007 Elsevier B.V. All rights reserved.
Chun-hsien Tseng - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of a new serotype of Duck Hepatitis Virus.
Virus research, 2007Co-Authors: Chun-hsien Tseng, Hsiang-jung TsaiAbstract:Duck Hepatitis strains 90D and 04G were determined to be antigenically unrelated to type 1 Duck Hepatitis Virus (DHV-1) by in vitro cross-neutralization assay. The genome sequences of 90D and 04G revealed that both strains of the new serotype DHV (N-DHV) possessed a typical picornaVirus genome organization apart from the unique possession of three in-tandem 2A genes present in DHV-1. The 2A1, 2A2, and 2A3 proteins represented an aphthoVirus-like 2A protein, AIG1-like protein, and human parechoVirus-like 2A protein, respectively. The N-DHV genome displayed unique features, compared to the DHV-1 genome. The 366 nt 3'UTR of N-DHV, the largest determined thus far among picornaViruses, was 52 nt longer than DHV-1. The pairwise percent identity of the nucleic acid and amino acid sequences at 1D region of N-DHV and DHV-1 were only 69.1-69.7 and 70.1-70.5%, respectively. Finally, phylogenetic and evolutionary analysis of N-DHV revealed that the N-DHV and DHV-1 belong to two different clusters of a novel genus in the Picornaviridae family.
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Molecular characterization of a new serotype of Duck Hepatitis Virus
Virus Research, 2007Co-Authors: Chun-hsien Tseng, Hsiang-jung TsaiAbstract:Duck Hepatitis strains 90D and 04G were determined to be antigenically unrelated to type 1 Duck Hepatitis Virus (DHV-1) by in vitro crossneutralization assay. The genome sequences of 90D and 04G revealed that both strains of the new serotype DHV (N-DHV) possessed a typical picornaVirus genome organization apart from the unique possession of three in-tandem 2A genes present in DHV-1. The 2A1, 2A2, and 2A3 proteins represented an aphthoVirus-like 2A protein, AIG1-like protein, and human parechoVirus-like 2A protein, respectively. The N-DHV genome displayed unique features, compared to the DHV-1 genome. The 366 nt 3 � UTR of N-DHV, the largest determined thus far among picornaViruses, was 52 nt longer than DHV-1. The pairwise percent identity of the nucleic acid and amino acid sequences at 1D region of N-DHV and DHV-1 were only 69.1–69.7 and 70.1–70.5%, respectively. Finally, phylogenetic and evolutionary analysis of N-DHV revealed that the N-DHV and DHV-1 belong to two different clusters of a novel genus in the Picornaviridae family. © 2007 Elsevier B.V. All rights reserved.
