The Experts below are selected from a list of 110160 Experts worldwide ranked by ideXlab platform
Jeffrey I Cohen - One of the best experts on this subject based on the ideXlab platform.
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varicella zoster virus open Reading Frame 2 encodes a membrane phosphoprotein that is dispensable for viral replication and for establishment of latency
2002Co-Authors: Hitoshi Sato, Lesley Pesnicak, Jeffrey I CohenAbstract:Varicella-zoster virus (VZV) encodes six genes that do not have homologs in herpes simplex virus. One of these genes, VZV open Reading Frame 2 (ORF2), was expressed as a 31-kDa phosphoprotein in the membranes of infected cells. Unlike equine and bovine herpesvirus type 1 ORF2 homologs that are associated with virions, VZV virions contained no detectable ORF2 protein. The ORF2 deletion mutant established a latent infection in cotton rats at a frequency and with a number of VZV genomes similar to that of the parental virus. ORF63 transcripts, a hallmark of latent infection, were present in ganglia latently infected with both the ORF2 deletion mutant and parental VZV. Thus, ORF2 is the first VZV gene shown to be dispensable for establishment of latent infection in an animal model.
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varicella zoster virus vzv virion associated transactivator open Reading Frame 62 protein enhances the infectivity of vzv dna
1994Co-Authors: Masako Moriuchi, Hiroyuki Moriuchi, Stephen E Straus, Jeffrey I CohenAbstract:Abstract Varicella-zoster virus (VZV) open Reading Frame (ORF) 62 protein (the homolog of herpes simplex virus type 1 (HSV-1) ICP4) and ORF10 protein (the homolog of HSV-1 VP16) are virion-associated transactivators. To investigate whether these proteins function during the initial stages of VZV infection, human melanoma cells were cotransfected with purified VZV DNA, devoid of any structural proteins, along with a plasmid expressing VZV ORF62 or ORF10 under the control of the human cytomegalovirus major immediate-early promoter. Expression of ORF62 enhanced the infectivity of VZV DNA up to 70-fold. In contrast, expression of ORF10 enhanced the infectivity of VZV DNA only threefold. These results show that high-level expression of ORF62 protein increases the probability that transfeeted VZV DNA will result in productive infection, suggesting that this virion-associeted transactivator (ORF62) has a critical role in initiating infection.
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varicella zoster virus vzv virion associated transactivator open Reading Frame 62 protein enhances the infectivity of vzv dna
1994Co-Authors: Masako Moriuchi, Hiroyuki Moriuchi, Stephen E Straus, Jeffrey I CohenAbstract:Abstract Varicella-zoster virus (VZV) open Reading Frame (ORF) 62 protein (the homolog of herpes simplex virus type 1 (HSV-1) ICP4) and ORF10 protein (the homolog of HSV-1 VP16) are virion-associated transactivators. To investigate whether these proteins function during the initial stages of VZV infection, human melanoma cells were cotransfected with purified VZV DNA, devoid of any structural proteins, along with a plasmid expressing VZV ORF62 or ORF10 under the control of the human cytomegalovirus major immediate-early promoter. Expression of ORF62 enhanced the infectivity of VZV DNA up to 70-fold. In contrast, expression of ORF10 enhanced the infectivity of VZV DNA only threefold. These results show that high-level expression of ORF62 protein increases the probability that transfeeted VZV DNA will result in productive infection, suggesting that this virion-associeted transactivator (ORF62) has a critical role in initiating infection.
Masako Moriuchi - One of the best experts on this subject based on the ideXlab platform.
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varicella zoster virus vzv virion associated transactivator open Reading Frame 62 protein enhances the infectivity of vzv dna
1994Co-Authors: Masako Moriuchi, Hiroyuki Moriuchi, Stephen E Straus, Jeffrey I CohenAbstract:Abstract Varicella-zoster virus (VZV) open Reading Frame (ORF) 62 protein (the homolog of herpes simplex virus type 1 (HSV-1) ICP4) and ORF10 protein (the homolog of HSV-1 VP16) are virion-associated transactivators. To investigate whether these proteins function during the initial stages of VZV infection, human melanoma cells were cotransfected with purified VZV DNA, devoid of any structural proteins, along with a plasmid expressing VZV ORF62 or ORF10 under the control of the human cytomegalovirus major immediate-early promoter. Expression of ORF62 enhanced the infectivity of VZV DNA up to 70-fold. In contrast, expression of ORF10 enhanced the infectivity of VZV DNA only threefold. These results show that high-level expression of ORF62 protein increases the probability that transfeeted VZV DNA will result in productive infection, suggesting that this virion-associeted transactivator (ORF62) has a critical role in initiating infection.
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varicella zoster virus vzv virion associated transactivator open Reading Frame 62 protein enhances the infectivity of vzv dna
1994Co-Authors: Masako Moriuchi, Hiroyuki Moriuchi, Stephen E Straus, Jeffrey I CohenAbstract:Abstract Varicella-zoster virus (VZV) open Reading Frame (ORF) 62 protein (the homolog of herpes simplex virus type 1 (HSV-1) ICP4) and ORF10 protein (the homolog of HSV-1 VP16) are virion-associated transactivators. To investigate whether these proteins function during the initial stages of VZV infection, human melanoma cells were cotransfected with purified VZV DNA, devoid of any structural proteins, along with a plasmid expressing VZV ORF62 or ORF10 under the control of the human cytomegalovirus major immediate-early promoter. Expression of ORF62 enhanced the infectivity of VZV DNA up to 70-fold. In contrast, expression of ORF10 enhanced the infectivity of VZV DNA only threefold. These results show that high-level expression of ORF62 protein increases the probability that transfeeted VZV DNA will result in productive infection, suggesting that this virion-associeted transactivator (ORF62) has a critical role in initiating infection.
Johan Den T Dunnen - One of the best experts on this subject based on the ideXlab platform.
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entries in the leiden duchenne muscular dystrophy mutation database an overview of mutation types and paradoxical cases that confirm the Reading Frame rule
2006Co-Authors: Annemieke Aartsmarus, Judith C T Van Deutekom, Ivo F A C Fokkema, Gertjan B Van Ommen, Johan Den T DunnenAbstract:The severe Duchenne and milder Becker muscular dystrophy are both caused by mutations in the DMD gene. This gene codes for dystrophin, a protein important for maintaining the stability of muscle-fiber membranes. In 1988, Monaco and colleagues postulated an explanation for the phenotypic difference between Duchenne and Becker patients in the Reading-Frame rule: In Duchenne patients, mutations induce a shift in the Reading Frame leading to prematurely truncated, dysfunctional dystrophins. In Becker patients, in-Frame mutations allow the synthesis of internally deleted, but largely functional dystrophins. Currently, over 4700 mutations have been reported in the Leiden DMD mutation database, of which 91% are in agreement with this rule. In this study we provide an update of the mutational variability in the DMD gene, particularly focusing on genotype-phenotype correlations and mutations that appear to be exceptions to the Reading-Frame rule.
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entries in the leiden duchenne muscular dystrophy mutation database an overview of mutation types and paradoxical cases that confirm the Reading Frame rule
2006Co-Authors: Annemieke Aartsmarus, Ivo F A C Fokkema, Judith C T Van Deutekom, Gertjan B Van Ommen, Johan Den T DunnenAbstract:The severe Duchenne and milder Becker muscular dystrophy are both caused by mutations in the DMD gene. This gene codes for dystrophin, a protein important for maintaining the stability of muscle-fiber membranes. In 1988, Monaco and colleagues postulated an explanation for the phenotypic difference between Duchenne and Becker patients in the Reading-Frame rule: In Duchenne patients, mutations induce a shift in the Reading Frame leading to prematurely truncated, dysfunctional dystrophins. In Becker patients, in-Frame mutations allow the synthesis of internally deleted, but largely functional dystrophins. Currently, over 4700 mutations have been reported in the Leiden DMD mutation database, of which 91% are in agreement with this rule. In this study we provide an update of the mutational variability in the DMD gene, particularly focusing on genotype–phenotype correlations and mutations that appear to be exceptions to the Reading-Frame rule. Muscle Nerve, 2006
Donald H. Gilden - One of the best experts on this subject based on the ideXlab platform.
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Expression of protein encoded by varicella-zoster virus open Reading Frame 63 in latently infected human ganglionic neurons.
1996Co-Authors: Ravy Mahalingam, Mary Wellish, Randall J. Cohrs, Serge Debrus, Jacques Piette, Bernard Rentier, Donald H. GildenAbstract:Abstract The ganglionic cell type in which varicella-zoster virus (VZV) is latent in humans was analyzed by using antibodies raised against in vitro-expressed VZV open Reading Frame 63 protein. VZV open Reading Frame 63 protein was detected exclusively in the cytoplasm of neurons of latently infected human trigeminal and thoracic ganglia. This is, to our knowledge, the first identification of a herpesvirus protein expressed during latency in the human nervous system.
Stephen E Straus - One of the best experts on this subject based on the ideXlab platform.
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varicella zoster virus vzv virion associated transactivator open Reading Frame 62 protein enhances the infectivity of vzv dna
1994Co-Authors: Masako Moriuchi, Hiroyuki Moriuchi, Stephen E Straus, Jeffrey I CohenAbstract:Abstract Varicella-zoster virus (VZV) open Reading Frame (ORF) 62 protein (the homolog of herpes simplex virus type 1 (HSV-1) ICP4) and ORF10 protein (the homolog of HSV-1 VP16) are virion-associated transactivators. To investigate whether these proteins function during the initial stages of VZV infection, human melanoma cells were cotransfected with purified VZV DNA, devoid of any structural proteins, along with a plasmid expressing VZV ORF62 or ORF10 under the control of the human cytomegalovirus major immediate-early promoter. Expression of ORF62 enhanced the infectivity of VZV DNA up to 70-fold. In contrast, expression of ORF10 enhanced the infectivity of VZV DNA only threefold. These results show that high-level expression of ORF62 protein increases the probability that transfeeted VZV DNA will result in productive infection, suggesting that this virion-associeted transactivator (ORF62) has a critical role in initiating infection.
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varicella zoster virus vzv virion associated transactivator open Reading Frame 62 protein enhances the infectivity of vzv dna
1994Co-Authors: Masako Moriuchi, Hiroyuki Moriuchi, Stephen E Straus, Jeffrey I CohenAbstract:Abstract Varicella-zoster virus (VZV) open Reading Frame (ORF) 62 protein (the homolog of herpes simplex virus type 1 (HSV-1) ICP4) and ORF10 protein (the homolog of HSV-1 VP16) are virion-associated transactivators. To investigate whether these proteins function during the initial stages of VZV infection, human melanoma cells were cotransfected with purified VZV DNA, devoid of any structural proteins, along with a plasmid expressing VZV ORF62 or ORF10 under the control of the human cytomegalovirus major immediate-early promoter. Expression of ORF62 enhanced the infectivity of VZV DNA up to 70-fold. In contrast, expression of ORF10 enhanced the infectivity of VZV DNA only threefold. These results show that high-level expression of ORF62 protein increases the probability that transfeeted VZV DNA will result in productive infection, suggesting that this virion-associeted transactivator (ORF62) has a critical role in initiating infection.
