The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Calvin J Coffey - One of the best experts on this subject based on the ideXlab platform.
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an appraisal of the computed axial tomographic appearance of the human mesentery based on mesenteric contiguity from the Duodenojejunal Flexure to the mesorectal level
European Radiology, 2016Co-Authors: Calvin J Coffey, Dara Walsh, Kevin Culligan, Leon G Walsh, Rishab Sehgal, Deirdre Mcgrath, Marie Staunton, Michael Moore, Colum P. Dunne, Timothy ScanlonAbstract:Objective The human mesentery is now regarded as contiguous from the Duodenojejunal (DJ) to anorectal level. This interpretation prompts re-appraisal of computed tomography (CT) images of the mesentery.
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mesenteric based surgery exploits gastrointestinal peritoneal mesenteric and fascial continuity from Duodenojejunal Flexure to the anorectal junction a review
Digestive Surgery, 2015Co-Authors: Calvin J Coffey, Dara Walsh, Mary F Dillon, Rishabh Sehgal, Peter Dockery, Fabio Quondamatteo, Leon G WalshAbstract:Introduction: It is now well established that mesenteric-based colorectal surgery is associated with superior outcomes. Recent anatomic observations have demonstr
Timothy Scanlon - One of the best experts on this subject based on the ideXlab platform.
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an appraisal of the computed axial tomographic appearance of the human mesentery based on mesenteric contiguity from the Duodenojejunal Flexure to the mesorectal level
European Radiology, 2016Co-Authors: Calvin J Coffey, Dara Walsh, Kevin Culligan, Leon G Walsh, Rishab Sehgal, Deirdre Mcgrath, Marie Staunton, Michael Moore, Colum P. Dunne, Timothy ScanlonAbstract:Objective The human mesentery is now regarded as contiguous from the Duodenojejunal (DJ) to anorectal level. This interpretation prompts re-appraisal of computed tomography (CT) images of the mesentery.
Leon G Walsh - One of the best experts on this subject based on the ideXlab platform.
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an appraisal of the computed axial tomographic appearance of the human mesentery based on mesenteric contiguity from the Duodenojejunal Flexure to the mesorectal level
European Radiology, 2016Co-Authors: Calvin J Coffey, Dara Walsh, Kevin Culligan, Leon G Walsh, Rishab Sehgal, Deirdre Mcgrath, Marie Staunton, Michael Moore, Colum P. Dunne, Timothy ScanlonAbstract:Objective The human mesentery is now regarded as contiguous from the Duodenojejunal (DJ) to anorectal level. This interpretation prompts re-appraisal of computed tomography (CT) images of the mesentery.
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mesenteric based surgery exploits gastrointestinal peritoneal mesenteric and fascial continuity from Duodenojejunal Flexure to the anorectal junction a review
Digestive Surgery, 2015Co-Authors: Calvin J Coffey, Dara Walsh, Mary F Dillon, Rishabh Sehgal, Peter Dockery, Fabio Quondamatteo, Leon G WalshAbstract:Introduction: It is now well established that mesenteric-based colorectal surgery is associated with superior outcomes. Recent anatomic observations have demonstr
Dara Walsh - One of the best experts on this subject based on the ideXlab platform.
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an appraisal of the computed axial tomographic appearance of the human mesentery based on mesenteric contiguity from the Duodenojejunal Flexure to the mesorectal level
European Radiology, 2016Co-Authors: Calvin J Coffey, Dara Walsh, Kevin Culligan, Leon G Walsh, Rishab Sehgal, Deirdre Mcgrath, Marie Staunton, Michael Moore, Colum P. Dunne, Timothy ScanlonAbstract:Objective The human mesentery is now regarded as contiguous from the Duodenojejunal (DJ) to anorectal level. This interpretation prompts re-appraisal of computed tomography (CT) images of the mesentery.
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mesenteric based surgery exploits gastrointestinal peritoneal mesenteric and fascial continuity from Duodenojejunal Flexure to the anorectal junction a review
Digestive Surgery, 2015Co-Authors: Calvin J Coffey, Dara Walsh, Mary F Dillon, Rishabh Sehgal, Peter Dockery, Fabio Quondamatteo, Leon G WalshAbstract:Introduction: It is now well established that mesenteric-based colorectal surgery is associated with superior outcomes. Recent anatomic observations have demonstr
Sawa Onouchi - One of the best experts on this subject based on the ideXlab platform.
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spatiotemporal distribution of extracellular matrix changes during mouse Duodenojejunal Flexure formation
Cell and Tissue Research, 2016Co-Authors: Sawa Onouchi, Osamu Ichii, Teppei Nakamura, Yaser Hosny Ali ElewaAbstract:Although gut Flexures characterize gut morphology, the mechanisms underlying Flexure formation remain obscure. Previously, we analyzed the mouse Duodenojejunal Flexure (DJF) as a model for its formation and reported asymmetric morphologies between the inner and outer bending sides of the fetal mouse DJF, implying their contribution to DJF formation. We now present the extracellular matrix (ECM) as an important factor for gut morphogenesis. We investigate ECM distribution during mouse DJF formation by histological techniques. In the intercellular space of the gut wall, high Alcian-Blue positivity for proteoglycans shifted from the outer to the inner side of the gut wall during DJF formation. Immunopositivity for fibronectin, collagen I, or pan-tenascin was higher at the inner than at the outer side. Collagen IV and laminins localized to the epithelial basement membrane. Beneath the mesothelium at the pre-formation stage, collagen IV and laminin immunopositivity showed inverse results, corresponding to the different cellular characteristics at this site. At the post-formation stage, however, laminin positivity beneath the mesothelium was the reverse of that observed during the pre-formation stage. High immunopositivity for collagen IV and laminins at the inner gut wall mesenchyme of the post-formation DJF implied a different blood vessel distribution. We conclude that ECM distribution changes spatiotemporally during mouse DJF formation, indicating ECM association with the establishment of asymmetric morphologies during this process.
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Asymmetric morphology of the cells comprising the inner and outer bending sides of the murine Duodenojejunal Flexure
Cell and Tissue Research, 2015Co-Authors: Sawa Onouchi, Osamu Ichii, Saori Otsuka-kanazawaAbstract:The asymmetric shape of component cells determines the asymmetric features of developing organs. Here, we focused on the murine Duodenojejunal Flexure (DJF), which bends without affecting the mesentery, and analyzed the morphological asymmetries of the mucosal epithelium and gut wall cells between the inner and outer bending sides at embryonic days 10.75–11.75. In the mucosal epithelium, the cell shape and the expression of epithelial markers (Cdx2, E-cadherin) showed no differences between the two DJF sides. In contrast, the gut wall cells comprising the inner and outer sides of the DJF were elongated along the inner–outer axis and perpendicular to this axis, respectively. Furthermore, the gut wall cells in the outer side possessed cytoplasmic processes connecting cells via adherens junctions, but those in the inner side were attached via adherens junctions of juxtaposed cell bodies and were relatively more crowded. In immunohistochemistry experiments, there was no remarkable difference in the positive reactions of markers for mesenchyme (vimentin), smooth muscle cells (αSMA), endothelial cells (LYVE-1, CD34), and undifferentiated neurons (Sox10) between the DJF sides. Interestingly, Tuj1-positive cells, indicating differentiated neurons, were observed in the middle layer of the gut wall, and these cells were significantly more abundant and tended to be larger in the inner side than in the outer side of the DJF. In conclusion, we clarified the asymmetries of gut wall cell morphology and neural differentiation between the inner and outer sides of the DJF. These characteristics of the developing murine DJF indicate its asymmetric formation.
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analysis of Duodenojejunal Flexure formation in mice implications for understanding the genetic basis for gastrointestinal morphology in mammals
Journal of Anatomy, 2013Co-Authors: Sawa Onouchi, Osamu Ichii, Saori Otsuka, Yoshiharu HashimotoAbstract:The mammalian gut undergoes morphological changes during development. We studied the developing mouse Duodenojejunal Flexure (DJF) to elucidate the mechanism of formation. During embryonic days 10.75–13.75, DJF formation was morphologically classified into three stages: the expansion stage, Flexure formation stage, and Flexure elongation stage. From the expansion to the Flexure formation stages, the DJF wall showed asymmetric morphology and proliferation along the left-right intestinal axis. From the Flexure formation to the Flexure elongation stage, the DJF started to bend dorsally with counterclockwise rotation along the antero-caudal intestinal axis, indicating that the original right side of the duodenum was rotated towards the dorsal body wall during development of the DJF. The direction of attachment of the dorsal mesentery to the DJF did not correspond to the bending direction of the DJF during Flexure formation, and this finding indicated that the dorsal mesentery contributed very little to DJF formation. During DJF formation, Aldh1a2 and hedgehog mRNAs were detected at the DJF, and their expression levels differed along the bending axis. In conclusion, DJF formation might be triggered by asymmetric morphology and proliferation along the left-right intestinal axis under the control of retinoic acid and hedgehog signaling.
