The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform
Norman L Eberhardt - One of the best experts on this subject based on the ideXlab platform.
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a novel Dynamin III isoform is up regulated in the central nervous system in hypothyroidism
International Journal of Developmental Neuroscience, 2003Co-Authors: Andrew M Arnold, Grant W Anderson, Bryan Mciver, Norman L EberhardtAbstract:Abstract Hypothyroidism in early postnatal development leads to abnormal CNS development that may be controlled in part at the level of gene transcription. Comparing the expression of euthyroid (EuT) and hypothyroid (HypoT) rat brain mRNAs by differential display PCR (ddPCR), we identified a novel Dynamin III mRNA that was up-regulated in the hypothyroid state. Northern analysis of brain mRNA using a probe from the Dynamin III open reading frame (ORF) revealed two transcripts of 3.0 and 7.2 kb size. The 3.0 kb transcript was observed in testis and brain, but not liver or lung RNA. In the brain the 3.0 kb transcript increased from 25 to 57% of adult (Ad) levels from postnatal day (p) p2–p15, but was not significantly regulated by thyroid hormone status. In contrast, the more abundant 7.2 kb transcript increased from 16.8 to 48.0% of adult levels from p2 to p15 in euthyroid rat pups but from 54.0% of adult levels at p2 to 97.9% of adult levels by p15 in hypothyroid pups. Overlapping cDNA clones from a rat brain cDNA library defined the 7.2 kb mRNA, which consisted of the complete ORF, containing a four amino acid insert at the end of the pleckstrin homology domain (PHD), and two unique 3′-flanking regions, that are likely derived from alternative processing. Thus, the 7.2 kb Dynamin III transcript is brain-specific and selectively regulated by thyroid hormone status. The data suggest that the regulation of Dynamin III by altered thyroid hormone status may affect synaptogenesis in the CNS through Dynamin’s essential roles in synaptic vesicle and receptor recycling, neurotransmitter reuptake, and growth factor receptor signaling.
Andrew M Arnold - One of the best experts on this subject based on the ideXlab platform.
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a novel Dynamin III isoform is up regulated in the central nervous system in hypothyroidism
International Journal of Developmental Neuroscience, 2003Co-Authors: Andrew M Arnold, Grant W Anderson, Bryan Mciver, Norman L EberhardtAbstract:Abstract Hypothyroidism in early postnatal development leads to abnormal CNS development that may be controlled in part at the level of gene transcription. Comparing the expression of euthyroid (EuT) and hypothyroid (HypoT) rat brain mRNAs by differential display PCR (ddPCR), we identified a novel Dynamin III mRNA that was up-regulated in the hypothyroid state. Northern analysis of brain mRNA using a probe from the Dynamin III open reading frame (ORF) revealed two transcripts of 3.0 and 7.2 kb size. The 3.0 kb transcript was observed in testis and brain, but not liver or lung RNA. In the brain the 3.0 kb transcript increased from 25 to 57% of adult (Ad) levels from postnatal day (p) p2–p15, but was not significantly regulated by thyroid hormone status. In contrast, the more abundant 7.2 kb transcript increased from 16.8 to 48.0% of adult levels from p2 to p15 in euthyroid rat pups but from 54.0% of adult levels at p2 to 97.9% of adult levels by p15 in hypothyroid pups. Overlapping cDNA clones from a rat brain cDNA library defined the 7.2 kb mRNA, which consisted of the complete ORF, containing a four amino acid insert at the end of the pleckstrin homology domain (PHD), and two unique 3′-flanking regions, that are likely derived from alternative processing. Thus, the 7.2 kb Dynamin III transcript is brain-specific and selectively regulated by thyroid hormone status. The data suggest that the regulation of Dynamin III by altered thyroid hormone status may affect synaptogenesis in the CNS through Dynamin’s essential roles in synaptic vesicle and receptor recycling, neurotransmitter reuptake, and growth factor receptor signaling.
Peter Roepstorff - One of the best experts on this subject based on the ideXlab platform.
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improved detection of hydrophilic phosphopeptides using graphite powder microcolumns and mass spectrometry evidence for in vivo doubly phosphorylated Dynamin i and Dynamin III
Molecular & Cellular Proteomics, 2004Co-Authors: Martin R. Larsen, Mark E Graham, Phillip J. Robinson, Peter RoepstorffAbstract:A common strategy in proteomics to improve the number and quality of peptides detected by mass spectrometry (MS) is to desalt and concentrate proteolytic digests using reversed phase (RP) chromatography prior to analysis. However, this does not allow for detection of small or hydrophilic peptides, or peptides altered in hydrophilicity such as phosphopeptides. We used microcolumns to compare the ability of RP resin or graphite powder to retain phosphopeptides. A number of standard phosphopeptides and a biologically relevant phosphoprotein, Dynamin I, were analyzed. MS revealed that some phosphopeptides did not bind the RP resin but were retained efficiently on the graphite. Those that did bind the RP resin often produced much stronger signals from the graphite powder. In particular, the method revealed a doubly phosphorylated peptide in a tryptic digest of Dynamin I purified from rat brain nerve terminals. The detection of this peptide was greatly enhanced by graphite micropurification. Sequencing by tandem MS confirmed the presence of phosphate at both Ser-774 and Ser-778, while a singly phosphorylated peptide was predominantly phosphorylated only on Ser-774. The method further revealed a singly and doubly phosphorylated peptide in Dynamin III, analogous to the Dynamin I sequence. A pair of Dynamin III phosphorylation sites were found at Ser-759 and Ser-763 by tandem MS. The results directly define the in vivo phosphorylation sites in Dynamins I and III for the first time. The findings indicate a large improvement in the detection of small amounts of phosphopeptides by MS and the approach has major implications for both small- and large-scale projects in phosphoproteomics.
Grant W Anderson - One of the best experts on this subject based on the ideXlab platform.
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a novel Dynamin III isoform is up regulated in the central nervous system in hypothyroidism
International Journal of Developmental Neuroscience, 2003Co-Authors: Andrew M Arnold, Grant W Anderson, Bryan Mciver, Norman L EberhardtAbstract:Abstract Hypothyroidism in early postnatal development leads to abnormal CNS development that may be controlled in part at the level of gene transcription. Comparing the expression of euthyroid (EuT) and hypothyroid (HypoT) rat brain mRNAs by differential display PCR (ddPCR), we identified a novel Dynamin III mRNA that was up-regulated in the hypothyroid state. Northern analysis of brain mRNA using a probe from the Dynamin III open reading frame (ORF) revealed two transcripts of 3.0 and 7.2 kb size. The 3.0 kb transcript was observed in testis and brain, but not liver or lung RNA. In the brain the 3.0 kb transcript increased from 25 to 57% of adult (Ad) levels from postnatal day (p) p2–p15, but was not significantly regulated by thyroid hormone status. In contrast, the more abundant 7.2 kb transcript increased from 16.8 to 48.0% of adult levels from p2 to p15 in euthyroid rat pups but from 54.0% of adult levels at p2 to 97.9% of adult levels by p15 in hypothyroid pups. Overlapping cDNA clones from a rat brain cDNA library defined the 7.2 kb mRNA, which consisted of the complete ORF, containing a four amino acid insert at the end of the pleckstrin homology domain (PHD), and two unique 3′-flanking regions, that are likely derived from alternative processing. Thus, the 7.2 kb Dynamin III transcript is brain-specific and selectively regulated by thyroid hormone status. The data suggest that the regulation of Dynamin III by altered thyroid hormone status may affect synaptogenesis in the CNS through Dynamin’s essential roles in synaptic vesicle and receptor recycling, neurotransmitter reuptake, and growth factor receptor signaling.
Bryan Mciver - One of the best experts on this subject based on the ideXlab platform.
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a novel Dynamin III isoform is up regulated in the central nervous system in hypothyroidism
International Journal of Developmental Neuroscience, 2003Co-Authors: Andrew M Arnold, Grant W Anderson, Bryan Mciver, Norman L EberhardtAbstract:Abstract Hypothyroidism in early postnatal development leads to abnormal CNS development that may be controlled in part at the level of gene transcription. Comparing the expression of euthyroid (EuT) and hypothyroid (HypoT) rat brain mRNAs by differential display PCR (ddPCR), we identified a novel Dynamin III mRNA that was up-regulated in the hypothyroid state. Northern analysis of brain mRNA using a probe from the Dynamin III open reading frame (ORF) revealed two transcripts of 3.0 and 7.2 kb size. The 3.0 kb transcript was observed in testis and brain, but not liver or lung RNA. In the brain the 3.0 kb transcript increased from 25 to 57% of adult (Ad) levels from postnatal day (p) p2–p15, but was not significantly regulated by thyroid hormone status. In contrast, the more abundant 7.2 kb transcript increased from 16.8 to 48.0% of adult levels from p2 to p15 in euthyroid rat pups but from 54.0% of adult levels at p2 to 97.9% of adult levels by p15 in hypothyroid pups. Overlapping cDNA clones from a rat brain cDNA library defined the 7.2 kb mRNA, which consisted of the complete ORF, containing a four amino acid insert at the end of the pleckstrin homology domain (PHD), and two unique 3′-flanking regions, that are likely derived from alternative processing. Thus, the 7.2 kb Dynamin III transcript is brain-specific and selectively regulated by thyroid hormone status. The data suggest that the regulation of Dynamin III by altered thyroid hormone status may affect synaptogenesis in the CNS through Dynamin’s essential roles in synaptic vesicle and receptor recycling, neurotransmitter reuptake, and growth factor receptor signaling.