Echinodermata

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Courtney L Smith - One of the best experts on this subject based on the ideXlab platform.

Jonathan V. Sweedler - One of the best experts on this subject based on the ideXlab platform.

  • Exploring the Sea Urchin Neuropeptide Landscape by Mass Spectrometry
    Journal of The American Society for Mass Spectrometry, 2018
    Co-Authors: Eric B. Monroe, Suresh P. Annangudi, Andinet A. Wadhams, Timothy A. Richmond, Ning Yang, Bruce R. Southey, Elena V. Romanova, Liliane Schoofs, Geert Baggerman, Jonathan V. Sweedler
    Abstract:

    Neuropeptides are essential cell-to-cell signaling messengers and serve important regulatory roles in animals. Although remarkable progress has been made in peptide identification across the Metazoa, for some phyla such as Echinodermata, limited neuropeptides are known and even fewer have been verified on the protein level. We employed peptidomic approaches using bioinformatics and mass spectrometry (MS) to experimentally confirm 23 prohormones and to characterize a new prohormone in nervous system tissue from Strongylocentrotus purpuratus , the purple sea urchin. Ninety-three distinct peptides from known and novel prohormones were detected with MS from extracts of the radial nerves, many of which are reported or experimentally confirmed here for the first time, representing a large-scale study of neuropeptides from the phylum Echinodermata. Many of the identified peptides and their precursor proteins have low homology to known prohormones from other species/phyla and are unique to the sea urchin. By pairing bioinformatics with MS, the capacity to characterize novel peptides and annotate prohormone genes is enhanced. Graphical Abstract

Gary M. Wessel - One of the best experts on this subject based on the ideXlab platform.

  • Phylogenomic Analyses of Echinodermata Support the Sister Groups of Asterozoa and Echinozoa
    PloS one, 2015
    Co-Authors: Adrian Reich, Casey W. Dunn, Koji Akasaka, Gary M. Wessel
    Abstract:

    Echinoderms (sea urchins, sea stars, brittle stars, sea lilies and sea cucumbers) are a group of diverse organisms, second in number within deuterostome species to only the chordates. Echinoderms serve as excellent model systems for developmental biology due to their diverse developmental mechanisms, tractable laboratory use, and close phylogenetic distance to chordates. In addition, echinoderms are very well represented in the fossil record, including some larval features, making echinoderms a valuable system for studying evolutionary development. The internal relationships of Echinodermata have not been consistently supported across phylogenetic analyses, however, and this has hindered the study of other aspects of their biology. In order to test echinoderm phylogenetic relationships, we sequenced 23 de novo transcriptomes from all five clades of echinoderms. Using multiple phylogenetic methods at a variety of sampling depths we have constructed a well-supported phylogenetic tree of Echinodermata, including support for the sister groups of Asterozoa (sea stars and brittle stars) and Echinozoa (sea urchins and sea cucumbers). These results will help inform developmental and evolutionary studies specifically in echinoderms and deuterostomes in general.

Chantal Conand - One of the best experts on this subject based on the ideXlab platform.

Sham V Nair - One of the best experts on this subject based on the ideXlab platform.