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Antonia Eliene Duarte - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon Echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs.
Molecules (Basel Switzerland), 2016Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, João Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs.
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antimicrobial activity and modulatory effect of essential oil from the leaf of rhaphiodon Echinus nees mart schauer on some antimicrobial drugs
Molecules, 2016Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, João Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs.
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estudo etnobotânico microbiologico e perfil toxicologico in vitro e in vivo de rhaphiodon Echinus nees mart schauer
2016Co-Authors: Antonia Eliene DuarteAbstract:As plantas medicinais tem sido muito utilizadas na medicina tradicional para o tratamento de varias doencas, especialmente porque tem baixo custo e baixa toxicidade em comparacao com as drogas sinteticas. O interesse toxico-farmacologicos destes produtos tem gerado grande interesse para a comunidade cientifica. Neste contexto, especies da familia Lamiaceae apresentam importâncias particulares, nao so por causa de suas aplicabilidades na industria de alimentos e cosmeticos, mas tambem devido ao seu uso etnofarmacologico. A especie Rhaphiodon Echinus (Nees & Mart) Schauer, popularmente conhecida como “betonica”, e distribuida na parte Nordeste do Brasil e as infusoes de suas folhas sao usadas como cha para o tratamento da inflamacao, infeccao microbiana e como agente antitussigeno. Apesar de seu uso, nao existe relatos sobre a toxicidade desta planta. Alem disso, nao ha nenhuma evidencia cientifica que justifica o uso terapeutico de R. Echinus na medicina popular. Sendo assim, o objetivo deste estudo foi investigar o perfil etnobotânico e toxicologico de R. Echinus em diferentes modelos experimentais, bem como avaliar seu perfil fitoquimico e seu potencial antioxidante em modelos in vitro. Particularmente, avaliamos o perfil toxicologico da infusao do extrato das folhas de R. Echinus (AEREi) em diferentes especies (Artemia salina, Rhamdia quelen, Rattus norvegicus) e o potencial antioxidante e citotoxico do extrato aquoso (EARE) e etanolico (EERE) das folhas de R. Echinus. Adicionalmente, o oleo essencial obtido a partir das folhas de R. Echinus foi testado sozinho e em combinacao com antifungico (fluconazol e nistatina) e antibioticos (gentamicina, amicacina, ciprofloxacina e imipenem) contra varias cepas bacterianas (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa) e fungicas (Candida albicans, Candida krusei and Candida tropicalis). Alem disso, a composicao quimica do oleo essencial bem como dos extratos (infusao (EAREi), EARE, EERE) foi avaliado pelo CG-MS e HPLC, respectivamente. Os resultados indicaram que os extratos (extratos infusao (EAREi), EARE, EERE) contem o acido galico, clorogenico, cafeico, elagico, e quercetina e rutina. O valor estimado de IC50 as 24 h e 48 h foi 181,70 μg/mL (124,62-264,93) and 100,63 μg/mL (71,01-142,59), respectivamente com a Artemia salina (intervalo de confianca de 95%). No entanto, a exposicao de jundia (Rhamdia quelen) a diferentes concentracoes de EAREi (1000-5000 mg/mL) nao causou mortalidade, revelando ausencia de atividade pro-oxidante, como observado em ensaios de geracao de especies reativas (RS) e de peroxidacao lipidica (PL) em brânquias, rim, figado e cerebro. Os niveis plasmaticos de alanina aminotransferase (ALT), alanina aminotransferase (ALT) e gama-glutamil transferase (GGT), indicaram que as diferentes concentracoes de EAREi nao foram hepatotoxicas para os jundias. Na segunda parte deste trabalho, os extratos aquosos (EARE) e etanolicos (EERE) de R. Echinus apresentaram atividade antioxidante evidenciado pela sequestramento do radical DPPH (IC50 = 111.9 μg/mL (EERE) and IC50 = 227.9 μg/mL (EARE)) e pelo seu potential de inibir a formacao de substâncias reativas ao acido tiobarbiturico (TBARS) induzido pelo Fe2+ (10 μM) nos homogeneizados de cerebro e figado de rato. EARE e EERE (30-480 μg/mL) nao induziram genotoxicidade, citotoxicidade ou fragilidade osmotica em celulas de sangue humano. Os resultados obtidos pelo metodo de microdiluicao revelaram baixa atividade antifungica e antibacteriana do oleo essencial das folhas de R. Echinus (MIC ≥ 1024 μg/mL). No entanto, ele foi capaz de modular a atividade das drogas antimicrobianas testadas, quando adicionado ao meio de crescimento na concentracao sub-inibitoria (ou seja, MIC/8). O oleo essencial das folhas de R. Echinus mostrou atividade quelante ao ferro (II), indicando que ele poderia ser de importância relevante no tratamento de doencas neurodegenerativas e infecciosas. O oleo essencial revelou a presenca de monoterpenos e sesquiterpenos, os quais podem ser pelo menos em parte, responsaveis pela acao moduladora do oleo, sendo um produto natural capaz de aumentar a atividade antibacteriana e antifungica, de medicamentos antimicrobianos. Contudo, os resultados sugerem que R. Echinus pode prevenir/proteger contra doencas associadas aos danos oxidativos, e indicam que o oleo essencial de suas folhas pode modular a atividade antibacteriana e antifungica de drogas antimicrobianas.
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Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon Echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs
MDPI AG, 2016Co-Authors: Antonia Eliene Duarte, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, Aline Boligon, João Batista Teixeira Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs
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Polyphenolic Composition and Evaluation of Antioxidant Activity, Osmotic Fragility and Cytotoxic Effects of Raphiodon Echinus (Nees & Mart.) Schauer.
Molecules (Basel Switzerland), 2015Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Katiane Roversi, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Francisco Assis Bezerra Da Cunha, Irwin Rose Alencar De Menezes, José Galberto Martins Da Costa, Adedayo O. AdemiluyiAbstract:Raphiodon Echinus (R. Echinus) is used in Brazilian folk medicine for the treatment of inflammation, coughs, and infectious diseases. However, no information is available on the potential antioxidant, cytotoxicity and genotoxicity of this plant. In this study, the polyphenolic constituents, antioxidant capacity and potential toxic effects of aqueous and ethanolic extracts of R. Echinus on human erythrocytes and leukocytes were investigated for the first time. R. Echinus extracts showed the presence of Gallic, chlorogenic, caffeic and ellagic acids, rutin, quercitrin and quercetin. Aqueous and ethanolic extracts of R. Echinus exhibited antioxidant activity in DPPH radical scavenging with IC50 = 111.9 μg/mL (EtOH extract) and IC50 = 227.9 μg/mL (aqueous extract). The extracts inhibited Fe2+ (10 μM) induced thiobarbituric acid reactive substances (TBARS) formation in rat brain and liver homogenates. The extracts (30–480 μg/mL) did not induce genotoxicity, cytotoxicity or osmotic fragility in human blood cells. The findings of this present study therefore suggest that the therapeutic effect of R. Echinus may be, in part, related to its antioxidant potential. Nevertheless, further in vitro and in vivo studies are required to ascertain the safety margin of its use in folk medicine.
Luiz Marivando Barros - One of the best experts on this subject based on the ideXlab platform.
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rhaphiodon Echinus nees mart schauer chemical toxicological activity and increased antibiotic activity of antifungal drug activity and antibacterial
Microbial Pathogenesis, 2017Co-Authors: Adrielle Rodrigues Costa, Aline Augusti Boligon, Luiz Marivando Barros, José Galberto Martins Da Costa, Kátia Regina Rodrigues Lima, Maria Ivaneide Rocha, Jean Paul Kamdem, Joara Nályda Pereira Carneiro, Juliana Lima Silva, Nadghia F. LeiteAbstract:The association of herbal products with standard antimicrobial drugs has recently gained more attention as a hope to overcome infectious diseases caused by multidrug-resistant microorganisms. Here, we investigated for the first time the antimicrobial (antifungal and antibacterial) activity of ethanolic and aqueous extracts of R. Echinus against multiresistant strains of bacteria (E. coli, P. aeruginosa and S. aureus) and fungi (C. albicans, C. krusei and C. tropicalis), as well as potential to enhance the activity of antibiotics drugs. In addition, both extract were chemically characterized and their toxicity was assessed in Artemia salina. Our results demonstrate that aqueous extract of R. Echinus caused a significant increase in the activity of antibiotics gentamicin and imipenem, while the ethanolic extract strongly enhanced the antibiotic activity of gentamicin, amikacin, imipenem and ciprofloxacin against P. aeruginosa. However, neither the ethanolic nor the aqueous extracts significantly affect the antibiotic activity of the drugs when tested against S. aureus. Phytochemical analysis of the extracts indicated ellagic acid, caffeic acid and chlorogenic acid as the major components which can be at least in part responsible for the enhanced activity of antibiotics. None of the extracts showed toxicity in A. salina even at the highest concentration tested (1000 μg/mL). All together, our results suggest that the leaf extract of R. Echinus can be an effective source of modulating agents.
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Rhaphiodon Echinus (Nees & Mart.) Schauer: Chemical, toxicological activity and increased antibiotic activity of antifungal drug activity and antibacterial
Microbial pathogenesis, 2017Co-Authors: Adrielle Rodrigues Costa, Aline Augusti Boligon, Luiz Marivando Barros, José Galberto Martins Da Costa, Juliana De Lima Silva, Kátia Regina Rodrigues Lima, Maria Ivaneide Rocha, Jean Paul Kamdem, Joara Nályda Pereira Carneiro, Nadghia F. LeiteAbstract:The association of herbal products with standard antimicrobial drugs has recently gained more attention as a hope to overcome infectious diseases caused by multidrug-resistant microorganisms. Here, we investigated for the first time the antimicrobial (antifungal and antibacterial) activity of ethanolic and aqueous extracts of R. Echinus against multiresistant strains of bacteria (E. coli, P. aeruginosa and S. aureus) and fungi (C. albicans, C. krusei and C. tropicalis), as well as potential to enhance the activity of antibiotics drugs. In addition, both extract were chemically characterized and their toxicity was assessed in Artemia salina. Our results demonstrate that aqueous extract of R. Echinus caused a significant increase in the activity of antibiotics gentamicin and imipenem, while the ethanolic extract strongly enhanced the antibiotic activity of gentamicin, amikacin, imipenem and ciprofloxacin against P. aeruginosa. However, neither the ethanolic nor the aqueous extracts significantly affect the antibiotic activity of the drugs when tested against S. aureus. Phytochemical analysis of the extracts indicated ellagic acid, caffeic acid and chlorogenic acid as the major components which can be at least in part responsible for the enhanced activity of antibiotics. None of the extracts showed toxicity in A. salina even at the highest concentration tested (1000 μg/mL). All together, our results suggest that the leaf extract of R. Echinus can be an effective source of modulating agents.
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Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon Echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs.
Molecules (Basel Switzerland), 2016Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, João Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs.
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antimicrobial activity and modulatory effect of essential oil from the leaf of rhaphiodon Echinus nees mart schauer on some antimicrobial drugs
Molecules, 2016Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, João Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs.
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Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon Echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs
MDPI AG, 2016Co-Authors: Antonia Eliene Duarte, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, Aline Boligon, João Batista Teixeira Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs
Aline Augusti Boligon - One of the best experts on this subject based on the ideXlab platform.
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Immunotoxicological Evaluation of Schinus molle L. (Anacardiaceae) Essential Oil in Lymphocytes and Macrophages
Evidence-Based Complementary and Alternative Medicine, 2018Co-Authors: Jonathaline Apollo Duarte, Léa Augusta De Bairros Zambrano, Luciane Dias Quintana, Mariana Balhego Rocha, Elizandra Gomes Schmitt, Aline Augusti Boligon, Marli Matiko Anraku De Campos, Luís Flávio Souza De Oliveira, Michel Mansur MachadoAbstract:Schinus molle L. is used to treat various diseases; however, the literature lacks information regarding its possible immunotoxic effects. The aim of the study was to investigate the immunotoxic effects of essential oil from leaves of Schinus molle L. in cultures of human lymphocytes and macrophages. The cultures were treated with essential oil (EO) of Schinus molle L. and subsequently subjected to genotoxic analysis (comet assay), mutagenic analysis (micronucleus frequency and chromosomal aberration), and cytotoxic (cell viability) and functional parameters (interleukins secretions). Our analyses have determined that the essential oil from leaves of Schinus molle L. presents several compounds with α-pinene being the major compound; in addition, the compound verbenene was firstly identified; genotoxic effects were detected only in macrophages and only at the two highest concentrations tested. An important finding is that Schinus molle L. oil causes an activation of the immune system. This action has its mechanism centered by the cascade nitric oxide-interleukin-10-tumor necrosis factor alpha.
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rhaphiodon Echinus nees mart schauer chemical toxicological activity and increased antibiotic activity of antifungal drug activity and antibacterial
Microbial Pathogenesis, 2017Co-Authors: Adrielle Rodrigues Costa, Aline Augusti Boligon, Luiz Marivando Barros, José Galberto Martins Da Costa, Kátia Regina Rodrigues Lima, Maria Ivaneide Rocha, Jean Paul Kamdem, Joara Nályda Pereira Carneiro, Juliana Lima Silva, Nadghia F. LeiteAbstract:The association of herbal products with standard antimicrobial drugs has recently gained more attention as a hope to overcome infectious diseases caused by multidrug-resistant microorganisms. Here, we investigated for the first time the antimicrobial (antifungal and antibacterial) activity of ethanolic and aqueous extracts of R. Echinus against multiresistant strains of bacteria (E. coli, P. aeruginosa and S. aureus) and fungi (C. albicans, C. krusei and C. tropicalis), as well as potential to enhance the activity of antibiotics drugs. In addition, both extract were chemically characterized and their toxicity was assessed in Artemia salina. Our results demonstrate that aqueous extract of R. Echinus caused a significant increase in the activity of antibiotics gentamicin and imipenem, while the ethanolic extract strongly enhanced the antibiotic activity of gentamicin, amikacin, imipenem and ciprofloxacin against P. aeruginosa. However, neither the ethanolic nor the aqueous extracts significantly affect the antibiotic activity of the drugs when tested against S. aureus. Phytochemical analysis of the extracts indicated ellagic acid, caffeic acid and chlorogenic acid as the major components which can be at least in part responsible for the enhanced activity of antibiotics. None of the extracts showed toxicity in A. salina even at the highest concentration tested (1000 μg/mL). All together, our results suggest that the leaf extract of R. Echinus can be an effective source of modulating agents.
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Rhaphiodon Echinus (Nees & Mart.) Schauer: Chemical, toxicological activity and increased antibiotic activity of antifungal drug activity and antibacterial
Microbial pathogenesis, 2017Co-Authors: Adrielle Rodrigues Costa, Aline Augusti Boligon, Luiz Marivando Barros, José Galberto Martins Da Costa, Juliana De Lima Silva, Kátia Regina Rodrigues Lima, Maria Ivaneide Rocha, Jean Paul Kamdem, Joara Nályda Pereira Carneiro, Nadghia F. LeiteAbstract:The association of herbal products with standard antimicrobial drugs has recently gained more attention as a hope to overcome infectious diseases caused by multidrug-resistant microorganisms. Here, we investigated for the first time the antimicrobial (antifungal and antibacterial) activity of ethanolic and aqueous extracts of R. Echinus against multiresistant strains of bacteria (E. coli, P. aeruginosa and S. aureus) and fungi (C. albicans, C. krusei and C. tropicalis), as well as potential to enhance the activity of antibiotics drugs. In addition, both extract were chemically characterized and their toxicity was assessed in Artemia salina. Our results demonstrate that aqueous extract of R. Echinus caused a significant increase in the activity of antibiotics gentamicin and imipenem, while the ethanolic extract strongly enhanced the antibiotic activity of gentamicin, amikacin, imipenem and ciprofloxacin against P. aeruginosa. However, neither the ethanolic nor the aqueous extracts significantly affect the antibiotic activity of the drugs when tested against S. aureus. Phytochemical analysis of the extracts indicated ellagic acid, caffeic acid and chlorogenic acid as the major components which can be at least in part responsible for the enhanced activity of antibiotics. None of the extracts showed toxicity in A. salina even at the highest concentration tested (1000 μg/mL). All together, our results suggest that the leaf extract of R. Echinus can be an effective source of modulating agents.
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Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon Echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs.
Molecules (Basel Switzerland), 2016Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, João Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs.
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antimicrobial activity and modulatory effect of essential oil from the leaf of rhaphiodon Echinus nees mart schauer on some antimicrobial drugs
Molecules, 2016Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, João Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs.
Diogo O. Souza - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon Echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs.
Molecules (Basel Switzerland), 2016Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, João Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs.
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antimicrobial activity and modulatory effect of essential oil from the leaf of rhaphiodon Echinus nees mart schauer on some antimicrobial drugs
Molecules, 2016Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, João Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs.
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Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon Echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs
MDPI AG, 2016Co-Authors: Antonia Eliene Duarte, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, Aline Boligon, João Batista Teixeira Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs
Irwin Rose Alencar De Menezes - One of the best experts on this subject based on the ideXlab platform.
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Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon Echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs.
Molecules (Basel Switzerland), 2016Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, João Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs.
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antimicrobial activity and modulatory effect of essential oil from the leaf of rhaphiodon Echinus nees mart schauer on some antimicrobial drugs
Molecules, 2016Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, João Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs.
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Antimicrobial Activity and Modulatory Effect of Essential Oil from the Leaf of Rhaphiodon Echinus (Nees & Mart) Schauer on Some Antimicrobial Drugs
MDPI AG, 2016Co-Authors: Antonia Eliene Duarte, Emily Pansera Waczuk, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Irwin Rose Alencar De Menezes, Maria Flaviana Bezerra Morais Braga, Nadghia F. Leite, Aline Boligon, João Batista Teixeira Rocha, Diogo O. SouzaAbstract:Background: Rhaphiodon Echinus is a weed plant used in the Brazilian folk medicinal for the treatment of infectious diseases. In this study, the essential oil of R. Echinus leaf was investigated for its antimicrobial properties. Methods: The chemical constituents of the essential oil were characterized by GC-MS. The antimicrobial properties were determined by studying by the microdilution method the effect of the oil alone, and in combination with antifungal or antibiotic drugs against the fungi Candida albicans, Candida krusei and Candida tropicalis and the microbes Escherichia coli, Staphylococcus aureus and Pseudomonas. In addition, the iron (II) chelation potential of the oil was determined. Results: The results showed the presence of β-caryophyllene and bicyclogermacrene in major compounds, and revealed a low antifungal and antibacterial activity of the essential oil, but a strong modulatory effect on antimicrobial drugs when associated with the oil. The essential oil showed iron (II) chelation activity. Conclusions: The GC-MS characterization revealed the presence of monoterpenes and sesquiterpenes in the essential oil and metal chelation potential, which may be responsible in part for the modulatory effect of the oil. These findings suggest that essential oil of R. Echinus is a natural product capable of enhancing the antibacterial and antifungal activity of antimicrobial drugs
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Polyphenolic Composition and Evaluation of Antioxidant Activity, Osmotic Fragility and Cytotoxic Effects of Raphiodon Echinus (Nees & Mart.) Schauer.
Molecules (Basel Switzerland), 2015Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Katiane Roversi, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Francisco Assis Bezerra Da Cunha, Irwin Rose Alencar De Menezes, José Galberto Martins Da Costa, Adedayo O. AdemiluyiAbstract:Raphiodon Echinus (R. Echinus) is used in Brazilian folk medicine for the treatment of inflammation, coughs, and infectious diseases. However, no information is available on the potential antioxidant, cytotoxicity and genotoxicity of this plant. In this study, the polyphenolic constituents, antioxidant capacity and potential toxic effects of aqueous and ethanolic extracts of R. Echinus on human erythrocytes and leukocytes were investigated for the first time. R. Echinus extracts showed the presence of Gallic, chlorogenic, caffeic and ellagic acids, rutin, quercitrin and quercetin. Aqueous and ethanolic extracts of R. Echinus exhibited antioxidant activity in DPPH radical scavenging with IC50 = 111.9 μg/mL (EtOH extract) and IC50 = 227.9 μg/mL (aqueous extract). The extracts inhibited Fe2+ (10 μM) induced thiobarbituric acid reactive substances (TBARS) formation in rat brain and liver homogenates. The extracts (30–480 μg/mL) did not induce genotoxicity, cytotoxicity or osmotic fragility in human blood cells. The findings of this present study therefore suggest that the therapeutic effect of R. Echinus may be, in part, related to its antioxidant potential. Nevertheless, further in vitro and in vivo studies are required to ascertain the safety margin of its use in folk medicine.
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polyphenolic composition and evaluation of antioxidant activity osmotic fragility and cytotoxic effects of raphiodon Echinus nees mart schauer
Molecules, 2015Co-Authors: Antonia Eliene Duarte, Aline Augusti Boligon, Emily Pansera Waczuk, Katiane Roversi, Maria Arlene Pessoa Da Silva, Luiz Marivando Barros, Francisco Assis Bezerra Da Cunha, Irwin Rose Alencar De Menezes, José Galberto Martins Da Costa, Adedayo O. AdemiluyiAbstract:Raphiodon Echinus (R. Echinus) is used in Brazilian folk medicine for the treatment of inflammation, coughs, and infectious diseases. However, no information is available on the potential antioxidant, cytotoxicity and genotoxicity of this plant. In this study, the polyphenolic constituents, antioxidant capacity and potential toxic effects of aqueous and ethanolic extracts of R. Echinus on human erythrocytes and leukocytes were investigated for the first time. R. Echinus extracts showed the presence of Gallic, chlorogenic, caffeic and ellagic acids, rutin, quercitrin and quercetin. Aqueous and ethanolic extracts of R. Echinus exhibited antioxidant activity in DPPH radical scavenging with IC50 = 111.9 μg/mL (EtOH extract) and IC50 = 227.9 μg/mL (aqueous extract). The extracts inhibited Fe2+ (10 μM) induced thiobarbituric acid reactive substances (TBARS) formation in rat brain and liver homogenates. The extracts (30–480 μg/mL) did not induce genotoxicity, cytotoxicity or osmotic fragility in human blood cells. The findings of this present study therefore suggest that the therapeutic effect of R. Echinus may be, in part, related to its antioxidant potential. Nevertheless, further in vitro and in vivo studies are required to ascertain the safety margin of its use in folk medicine.
