The Experts below are selected from a list of 168 Experts worldwide ranked by ideXlab platform
Terukazu Nagumo - One of the best experts on this subject based on the ideXlab platform.
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Effects of a fucoidan on the activation of plasminogen by u-PA and t-PA.
Thrombosis research, 2000Co-Authors: Takashi Nishino, Terukazu Nagumo, Tomoko Yamauchi, Mariko Horie, Haruo SuzukiAbstract:The effect of an anticoagulant fucoidan (C-I-H) from the brown seaweed Ecklonia kurome on the fibrinolytic system was studied in vitro using S-2251 as a substrate of plasmin. C-I-H enhanced the activation of Glu- and Lys-plasminogen by high molecular weight urokinase-type plasminogen activator (HMW u-PA) very effectively, but the activation by low molecular weight u-PA was hardly enhanced with C-I-H. C-I-H also potentiated moderately the activation by single- and two-chain tissue-type plasminogen activators (sct- and tct-PA). These effects of C-I-H were higher than those of heparin used. But C-I-H had no effect on the amidolytic activity of plasmin to S-2251. These results indicate that C-I-H promotes the generation of plasmin in the plasminogen activation by HMW u-PA and t-PA, but not the activity of generated plasmin. Kinetic analyses suggest that C-I-H enhances the HMW u-PA-mediated plasminogen activation by increasing the affinity of the activator for Glu- and Lys-plasminogen and by increasing the molecular activity of the activator. On the other hand, C-I-H had no effect on the affinity of tct-PA for both plasminogens. The catalytic efficiencies of HMW u-PA and tct-PA for the activation of both plasminogens were increased with C-I-H about 8- and 2-fold, respectively. The present results suggest that C-I-H has the fibrinolytic activity by stimulating the plasminogen activation by HMW u-PA and t-PA. The mechanism of the enhancement effect of C-I-H on the activation is presumed to be that C-I-H binds to plasminogen, thereby inducing a structural change of plasminogen susceptible to the action of plasminogen activators.
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Inhibition of the generation of thrombin and factor Xa by a fucoidan from the brown seaweed Ecklonia kurome.
Thrombosis research, 1999Co-Authors: Takashi Nishino, Terukazu Nagumo, Michio Fujihara, Akihiro Fukuda, Eisuke KajiAbstract:The effects of a fucoidan (C-II), which was purified from the brown seaweed Ecklonia kurome, on the generation of thrombin and factor Xa have been investigated by measuring the amidolytic activities by using the respective specific chromogenic substrates in both plasma and purified systems. C-II inhibited significantly the generation of thrombin in both the intrinsic and the extrinsic pathways, although the intrinsic inhibitory effect by C-II was more remarkable than the extrinsic one. On the other hand, C-II was a good inhibitor of the factor Xa generation in the intrinsic pathway, while it was a poor one in the extrinsic pathway. In the purified systems C-II also inhibited the formation of prothrombin-activating complex (i.e., prothrombinase), but not its activity. The concentration of C-II required for 50% inhibition of thrombin generation was about one-tenth to one-seventh of that of the activity of the generated thrombin in plasma. These results indicate that C-II has an inhibitory effect on the generation of thrombin by blocking the formation of prothrombinase and by preventing the generation of intrinsic factor Xa in addition to its antithrombin activity, and also that the generation-inhibitory effect is more remarkable than C-II's enhancement effect on the antithrombin activity by heparin cofactor II in plasma.
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Anticoagulant and antithrombin activities of oversulfated fucans
Carbohydrate research, 1992Co-Authors: Takashi Nishino, Terukazu NagumoAbstract:Abstract Three species of oversulfated fucans having different sulfate contents (the ratio of sulfate/total sugar residues, 1.38–1.98) were prepared by chemical sulfation of a fucan sulfate (sulfate/sugar ratio, 1.28) isolated from the brown seaweed Ecklonia kurome . The anticoagulant activities of the oversulfated fucans were compared with that of a parent fucan with respect to activated partial thromboplastin time (APTT) and thrombin time (TT) in plasma. The respective activities (for (APTT and TT) of the oversulfated fucans increased to 110–119% and 108–140% of the original values increased in their sulfate content. The anticoagulant activity with respect to APTT (173 units/mg) of an oversulfated fucan (sulfate/sugar ratio, 1.98) was higher than that (167 units/mg) of heparin used as a standard. The heparin cofactor II-mediated antithrombin activity of the oversulfated fucans also increased significantly with increase in sulfate content. The maximum activity was higher than those of the parent fucan and heparin. However, the increment of the anticoagulant and the antithrombin effects gradually decreased with increase in the sulfate content of the fucans. These results indicate that the effects of the fucan sulfate are dependent on its sulfate content until a plateau is reached.
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The influence of sulfate content and molecular weight of a fucan sulfate from the brown seaweed Ecklonia kurome on its antithrombin activity.
Thrombosis research, 1991Co-Authors: Takashi Nishino, Y. Aizu, Terukazu NagumoAbstract:The antithrombin effects of the sub-fractionated fucans with different molecular weights and sulfate contents, which were prepared from a fucan sulfate isolated from the brown seaweed Ecklonia kurome, were examined for their abilities to inhibit thrombin-fibrinogen reaction and amidolytic activity of thrombin, and to bind to fibrinogen. The inhibitory effects of the fucans on both fibrinogen clotting by thrombin and amidolysis of the protein in the presence of heparin cofactor II were improved with increase in their molecular weights and reduced with decrease in their sulfate contents. The binding abilities of the fucans with almost the same sulfate content to fibrinogen were unchanged independently of their molecular weights, although the ability diminished with decrease in the sulphate content. These results suggest that heparin cofactor II-mediated antithrombin activity of the fucan sulfate is dependent on both its sulfate content and molecular weight, and also that the inhibitory effect of the polysaccharide on fibrinogen clotting by thrombin may be attributable to the steric hindrance by its binding to fibrinogen.
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Antithrombin activity of a fucan sulfate from the brown seaweed Ecklonia kurome.
Thrombosis research, 1991Co-Authors: Takashi Nishino, Y. Aizu, Terukazu NagumoAbstract:The mechanism of antithrombin action of a fucan sulfate (C-II), which was isolated from the brown seaweed Ecklonia kurome, was examined by clotting method using a thrombin-fibrinogen system and by amidolytic method using a chromogenic substrate in the presence and the absence of antithrombin III (AT III) or heparin cofactor II (HG II). C-II significantly inhibited the clotting of fibrinogen by thrombin even in the absence of the protease inhibitors, and the anidolytic activity of the protein only in the presence of HC III. C-III was not adsorbed on an AT III-agarose column and its anticoagulant activity in AT III-depleted plasma was the same as that in normal one. Examination of interaction of C-III with fibrinogen by g-el filtration chromatography demonstrated that C-III bound to the protein. These results indicated that the antithrombin activity of C-III was mediated by HC III and not by AT III, and that the polysaceharide bound to fibrinogen, thereby blocking thrombin action, and also that its direct thrombin inhibition was very weak.
Takashi Nishino - One of the best experts on this subject based on the ideXlab platform.
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Effects of a fucoidan on the activation of plasminogen by u-PA and t-PA.
Thrombosis research, 2000Co-Authors: Takashi Nishino, Terukazu Nagumo, Tomoko Yamauchi, Mariko Horie, Haruo SuzukiAbstract:The effect of an anticoagulant fucoidan (C-I-H) from the brown seaweed Ecklonia kurome on the fibrinolytic system was studied in vitro using S-2251 as a substrate of plasmin. C-I-H enhanced the activation of Glu- and Lys-plasminogen by high molecular weight urokinase-type plasminogen activator (HMW u-PA) very effectively, but the activation by low molecular weight u-PA was hardly enhanced with C-I-H. C-I-H also potentiated moderately the activation by single- and two-chain tissue-type plasminogen activators (sct- and tct-PA). These effects of C-I-H were higher than those of heparin used. But C-I-H had no effect on the amidolytic activity of plasmin to S-2251. These results indicate that C-I-H promotes the generation of plasmin in the plasminogen activation by HMW u-PA and t-PA, but not the activity of generated plasmin. Kinetic analyses suggest that C-I-H enhances the HMW u-PA-mediated plasminogen activation by increasing the affinity of the activator for Glu- and Lys-plasminogen and by increasing the molecular activity of the activator. On the other hand, C-I-H had no effect on the affinity of tct-PA for both plasminogens. The catalytic efficiencies of HMW u-PA and tct-PA for the activation of both plasminogens were increased with C-I-H about 8- and 2-fold, respectively. The present results suggest that C-I-H has the fibrinolytic activity by stimulating the plasminogen activation by HMW u-PA and t-PA. The mechanism of the enhancement effect of C-I-H on the activation is presumed to be that C-I-H binds to plasminogen, thereby inducing a structural change of plasminogen susceptible to the action of plasminogen activators.
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Inhibition of the generation of thrombin and factor Xa by a fucoidan from the brown seaweed Ecklonia kurome.
Thrombosis research, 1999Co-Authors: Takashi Nishino, Terukazu Nagumo, Michio Fujihara, Akihiro Fukuda, Eisuke KajiAbstract:The effects of a fucoidan (C-II), which was purified from the brown seaweed Ecklonia kurome, on the generation of thrombin and factor Xa have been investigated by measuring the amidolytic activities by using the respective specific chromogenic substrates in both plasma and purified systems. C-II inhibited significantly the generation of thrombin in both the intrinsic and the extrinsic pathways, although the intrinsic inhibitory effect by C-II was more remarkable than the extrinsic one. On the other hand, C-II was a good inhibitor of the factor Xa generation in the intrinsic pathway, while it was a poor one in the extrinsic pathway. In the purified systems C-II also inhibited the formation of prothrombin-activating complex (i.e., prothrombinase), but not its activity. The concentration of C-II required for 50% inhibition of thrombin generation was about one-tenth to one-seventh of that of the activity of the generated thrombin in plasma. These results indicate that C-II has an inhibitory effect on the generation of thrombin by blocking the formation of prothrombinase and by preventing the generation of intrinsic factor Xa in addition to its antithrombin activity, and also that the generation-inhibitory effect is more remarkable than C-II's enhancement effect on the antithrombin activity by heparin cofactor II in plasma.
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Anticoagulant and antithrombin activities of oversulfated fucans
Carbohydrate research, 1992Co-Authors: Takashi Nishino, Terukazu NagumoAbstract:Abstract Three species of oversulfated fucans having different sulfate contents (the ratio of sulfate/total sugar residues, 1.38–1.98) were prepared by chemical sulfation of a fucan sulfate (sulfate/sugar ratio, 1.28) isolated from the brown seaweed Ecklonia kurome . The anticoagulant activities of the oversulfated fucans were compared with that of a parent fucan with respect to activated partial thromboplastin time (APTT) and thrombin time (TT) in plasma. The respective activities (for (APTT and TT) of the oversulfated fucans increased to 110–119% and 108–140% of the original values increased in their sulfate content. The anticoagulant activity with respect to APTT (173 units/mg) of an oversulfated fucan (sulfate/sugar ratio, 1.98) was higher than that (167 units/mg) of heparin used as a standard. The heparin cofactor II-mediated antithrombin activity of the oversulfated fucans also increased significantly with increase in sulfate content. The maximum activity was higher than those of the parent fucan and heparin. However, the increment of the anticoagulant and the antithrombin effects gradually decreased with increase in the sulfate content of the fucans. These results indicate that the effects of the fucan sulfate are dependent on its sulfate content until a plateau is reached.
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The influence of sulfate content and molecular weight of a fucan sulfate from the brown seaweed Ecklonia kurome on its antithrombin activity.
Thrombosis research, 1991Co-Authors: Takashi Nishino, Y. Aizu, Terukazu NagumoAbstract:The antithrombin effects of the sub-fractionated fucans with different molecular weights and sulfate contents, which were prepared from a fucan sulfate isolated from the brown seaweed Ecklonia kurome, were examined for their abilities to inhibit thrombin-fibrinogen reaction and amidolytic activity of thrombin, and to bind to fibrinogen. The inhibitory effects of the fucans on both fibrinogen clotting by thrombin and amidolysis of the protein in the presence of heparin cofactor II were improved with increase in their molecular weights and reduced with decrease in their sulfate contents. The binding abilities of the fucans with almost the same sulfate content to fibrinogen were unchanged independently of their molecular weights, although the ability diminished with decrease in the sulphate content. These results suggest that heparin cofactor II-mediated antithrombin activity of the fucan sulfate is dependent on both its sulfate content and molecular weight, and also that the inhibitory effect of the polysaccharide on fibrinogen clotting by thrombin may be attributable to the steric hindrance by its binding to fibrinogen.
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Antithrombin activity of a fucan sulfate from the brown seaweed Ecklonia kurome.
Thrombosis research, 1991Co-Authors: Takashi Nishino, Y. Aizu, Terukazu NagumoAbstract:The mechanism of antithrombin action of a fucan sulfate (C-II), which was isolated from the brown seaweed Ecklonia kurome, was examined by clotting method using a thrombin-fibrinogen system and by amidolytic method using a chromogenic substrate in the presence and the absence of antithrombin III (AT III) or heparin cofactor II (HG II). C-II significantly inhibited the clotting of fibrinogen by thrombin even in the absence of the protease inhibitors, and the anidolytic activity of the protein only in the presence of HC III. C-III was not adsorbed on an AT III-agarose column and its anticoagulant activity in AT III-depleted plasma was the same as that in normal one. Examination of interaction of C-III with fibrinogen by g-el filtration chromatography demonstrated that C-III bound to the protein. These results indicated that the antithrombin activity of C-III was mediated by HC III and not by AT III, and that the polysaceharide bound to fibrinogen, thereby blocking thrombin action, and also that its direct thrombin inhibition was very weak.
Takashi Kuda - One of the best experts on this subject based on the ideXlab platform.
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Anti-glycation properties of the aqueous extract solutions of dried algae products and effect of lactic acid fermentation on the properties.
Food chemistry, 2015Co-Authors: Takashi Kuda, Mika Eda, Manami Kataoka, Maki Nemoto, Miho Kawahara, Satoshi Oshio, Hajime Takahashi, Bon KimuraAbstract:The antioxidant and anti-glycation properties in aqueous extract solutions (AESs) of 11 dried algae products were investigated. AESs of brown algae Ecklonia kurome (kurome) and Ecklonia stolonifera (tsuruarame) showed a strong DPPH radical-scavenging capacity and Fe-reducing power with high total phenolic compound content. On the other hand, superoxide anion radical-scavenging capacities of Porphyra sp. (iwanori, red alga), sporophyll of Undaria pinnatifida (mekabu, brown alga), and Gelidiaceae sp. (tengusa, red alga) were also high. Anti-glycation activities in BSA-fructose and BSA-methylglyoxal glycation were also high in kurome, while iwanori showed high activity. Results of the BSA-fructose model agreed with those of superoxide anion radical-scavenging. On the other hand, those of the BSA-methylglyoxal model agreed with those of the phenolic content, DPPH radical-scavenging capacity, and Fe-reducing power. Anti-glycation activities of iwanori, U. pinnatifida (wakame), and mekabu in the BSA-fructose model were clearly increased by fermentation with Lactobacillus plantarum AN6.
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Minerals, polysaccharides and antioxidant properties of aqueous solutions obtained from macroalgal beach-casts in the Noto Peninsula, Ishikawa, Japan
Food Chemistry, 2009Co-Authors: Takashi Kuda, Takahiko IkemoriAbstract:Abstract To clarify the useful properties of the macroalgal beach-casts for food, we determined mineral, water-soluble polysaccharide and total phenolic compound contents and antioxidant properties of aqueous solutions obtained from frond of eight brown and four green algae. These algae were washed ashore from the East Sea (the Sea of Japan) to the northeast beaches of the Noto Peninsula in autumn. There were fresh and large amounts of Ecklonia stolonifera (Es), Ecklonia kurome (Ek), Sargassum ringgoldianum subsp. coreanum (Sr) and Sargassum macrocarpum on the beaches. Potassium, magnesium and calcium ions were high in Sr-solution. High molecular weight water-soluble polysaccharide, mainly alginates, and relative viscosity were also high in Sr-solution. Both Es and Ek solutions showed high contents of total phenolic compounds and high antioxidant activities, including DPPH radical- and hydroxyl radical-scavenging and ferrous reducing power. On the other hand, the highest superoxide anion radical-scavenging activity was in the Sr solution. In the case of Sr, the same antioxidant properties were also found in the aqueous solution obtained from stem. These results suggest that some macroalgal beach-casts, especially Sr, can be utilised as new natural resources for functional foods, cosmetics, medical applications and fertilizer instead of being processed to landfill or incineration.
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Varieties of antioxidant and antibacterial properties of Ecklonia stolonifera and Ecklonia kurome products harvested and processed in the Noto peninsula, Japan
Food Chemistry, 2007Co-Authors: Takashi Kuda, Taeko Kunii, Hideyuki Goto, Takamoto Suzuki, Toshihiro YanoAbstract:Abstract Ecklonia stolonifera and Ecklonia kurome are traditional edible brown algae in the fisheries towns in Far East Asia. In the Noto Peninsula area, Ishikawa, Japan, both the Ecklonia are called ‘kajime’ and people believe that the algae improve the property of blood. To determine the varieties of antioxidant and antibacterial properties E. stolonifera and E. kurome products, assays for total phenolic content and antioxidant activities, including DPPH radical-scavenging activity, superoxide anion radical-generated by non-enzymatic system, ferrous-reducing power and WST-8, a tetrazolium salt, redox activity of four dried and two boiled E. stolonifera , and four dried and two raw E. kurome preparations were tested in this study. Furthermore, antibacterial activity of the products was tested. Though the total phenolic content, the antioxidant activity and the antibacterial activities of E. stolorifera and E. kurome products were high, these properties were varied by manufacturers or each product. Especially, two dried and two boiled products of E. stolonifera showed low activities. The results of this study indicate that the contents of functional compounds and their activities were affected or decreased greatly by the processing method.
Sung Hyun Yang - One of the best experts on this subject based on the ideXlab platform.
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description of croceitalea gen nov in the family flavobacteriaceae with two species croceitalea Eckloniae sp nov and croceitalea dokdonensis sp nov isolated from the rhizosphere of the marine alga Ecklonia kurome
International Journal of Systematic and Evolutionary Microbiology, 2008Co-Authors: Kae Kyoung Kwon, Sung Hyun Yang, Chan Hong ParkAbstract:Two novel bacterial strains, designated DOKDO 025T and DOKDO 023T, were isolated on Dokdo Island, Korea, from the rhizosphere of the brown alga Ecklonia kurome. The strains were subjected to a polyphasic taxonomy study and were found to be Gram-negative, aerobic, rod-shaped, non-motile and orange-coloured. The isolates shared 96.3 % 16S rRNA gene sequence similarity. They showed 93.8–95.6 % 16S rRNA gene sequence similarity with respect to members of the genus Muricauda in the family Flavobacteriaceae, but formed a distinct phyletic line. Moreover, the cellular appendages reported for all Muricauda species were absent from strains DOKDO 025T and DOKDO 023T. The predominant cellular fatty acids of strain DOKDO 025T were iso-C15 : 0, iso-C15 : 1 and one with an equivalent chain-length of 13.565 and those of strain DOKDO 023T were iso-C15 : 0, iso-C15 : 1 and iso-C17 : 0 3-OH. The DNA G+C content of strains DOKDO 025T and DOKDO 023T were 59.5 and 66.5 mol%, respectively, higher than any values found in recognized members of the family Flavobacteriaceae. The major respiratory quinone was MK-6. On the basis of evidence from the polyphasic study, strains DOKDO 025T and DOKDO 023T represent two novel species in a new genus, Croceitalea gen. nov., for which the names Croceitalea Eckloniae sp. nov. (the type species) and Croceitalea dokdonensis sp. nov. are proposed. The type strain of Croceitalea Eckloniae sp. nov. is DOKDO 025T (=KCCM 42309T =JCM 13827T) and that of Croceitalea dokdonensis sp. nov. is DOKDO 023T (=KCCM 42308T =JCM 13826T).
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flagellimonas Eckloniae gen nov sp nov a mesophilic marine bacterium of the family flavobacteriaceae isolated from the rhizosphere of Ecklonia kurome
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Kae Kyoung Kwon, Sung Hyun YangAbstract:A marine bacterium, DOKDO 007T, was isolated from the rhizosphere of the marine alga Ecklonia kurome collected from Dokdo Island, Korea, in October 2004. The strain produced orange-coloured colonies on marine agar 2216. 16S rRNA gene sequence analysis indicated that the novel isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarities with members of the genus Muricauda (92.0–94.0 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genera Muricauda and Costertonia. Cells were aerobic, Gram-negative rods producing non-diffusible carotenoid pigments. In contrast to all other members of the family Flavobacteriaceae, cells of DOKDO 007T were motile by means of a polar flagellum. Optimal growth occurred in the presence of 3.5–4 % (w/v) sea salts (corresponding to 2.7–3.1 % NaCl), at pH 8 and at temperatures of 26–29 °C. The novel strain required Ca2+ ions in addition to NaCl for growth. The dominant fatty acids were iso-15 : 0, iso-15 : 1ω10c and 10-methyl-16 : 0. The major respiratory quinone was MK-6. The DNA G+C content was 56.3 mol%, an unusually high value for members of the family Flavobacteriaceae. On the basis of these polyphasic taxonomic data, strain DOKDO 007T should be classified as representing a new genus and novel species in the family Flavobacteriaceae, for which the name Flagellimonas Eckloniae gen. nov., sp. nov. is proposed. The type strain is DOKDO 007T (=KCCM 42307T=JCM 13831T).
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Flagellimonas Eckloniae gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from the rhizosphere of Ecklonia kurome.
International journal of systematic and evolutionary microbiology, 2007Co-Authors: Seung Seob Bae, Kae Kyoung Kwon, Sung Hyun Yang, Hee-soon Lee, Sang-jin Kim, Jung-hyun LeeAbstract:A marine bacterium, DOKDO 007(T), was isolated from the rhizosphere of the marine alga Ecklonia kurome collected from Dokdo Island, Korea, in October 2004. The strain produced orange-coloured colonies on marine agar 2216. 16S rRNA gene sequence analysis indicated that the novel isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarities with members of the genus Muricauda (92.0-94.0 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genera Muricauda and Costertonia. Cells were aerobic, Gram-negative rods producing non-diffusible carotenoid pigments. In contrast to all other members of the family Flavobacteriaceae, cells of DOKDO 007(T) were motile by means of a polar flagellum. Optimal growth occurred in the presence of 3.5-4 % (w/v) sea salts (corresponding to 2.7-3.1 % NaCl), at pH 8 and at temperatures of 26-29 degrees C. The novel strain required Ca(2+) ions in addition to NaCl for growth. The dominant fatty acids were iso-15 : 0, iso-15 : 1omega10c and 10-methyl-16 : 0. The major respiratory quinone was MK-6. The DNA G+C content was 56.3 mol%, an unusually high value for members of the family Flavobacteriaceae. On the basis of these polyphasic taxonomic data, strain DOKDO 007(T) should be classified as representing a new genus and novel species in the family Flavobacteriaceae, for which the name Flagellimonas Eckloniae gen. nov., sp. nov. is proposed. The type strain is DOKDO 007(T) (=KCCM 42307(T)=JCM 13831(T)).
Kae Kyoung Kwon - One of the best experts on this subject based on the ideXlab platform.
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description of croceitalea gen nov in the family flavobacteriaceae with two species croceitalea Eckloniae sp nov and croceitalea dokdonensis sp nov isolated from the rhizosphere of the marine alga Ecklonia kurome
International Journal of Systematic and Evolutionary Microbiology, 2008Co-Authors: Kae Kyoung Kwon, Sung Hyun Yang, Chan Hong ParkAbstract:Two novel bacterial strains, designated DOKDO 025T and DOKDO 023T, were isolated on Dokdo Island, Korea, from the rhizosphere of the brown alga Ecklonia kurome. The strains were subjected to a polyphasic taxonomy study and were found to be Gram-negative, aerobic, rod-shaped, non-motile and orange-coloured. The isolates shared 96.3 % 16S rRNA gene sequence similarity. They showed 93.8–95.6 % 16S rRNA gene sequence similarity with respect to members of the genus Muricauda in the family Flavobacteriaceae, but formed a distinct phyletic line. Moreover, the cellular appendages reported for all Muricauda species were absent from strains DOKDO 025T and DOKDO 023T. The predominant cellular fatty acids of strain DOKDO 025T were iso-C15 : 0, iso-C15 : 1 and one with an equivalent chain-length of 13.565 and those of strain DOKDO 023T were iso-C15 : 0, iso-C15 : 1 and iso-C17 : 0 3-OH. The DNA G+C content of strains DOKDO 025T and DOKDO 023T were 59.5 and 66.5 mol%, respectively, higher than any values found in recognized members of the family Flavobacteriaceae. The major respiratory quinone was MK-6. On the basis of evidence from the polyphasic study, strains DOKDO 025T and DOKDO 023T represent two novel species in a new genus, Croceitalea gen. nov., for which the names Croceitalea Eckloniae sp. nov. (the type species) and Croceitalea dokdonensis sp. nov. are proposed. The type strain of Croceitalea Eckloniae sp. nov. is DOKDO 025T (=KCCM 42309T =JCM 13827T) and that of Croceitalea dokdonensis sp. nov. is DOKDO 023T (=KCCM 42308T =JCM 13826T).
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flagellimonas Eckloniae gen nov sp nov a mesophilic marine bacterium of the family flavobacteriaceae isolated from the rhizosphere of Ecklonia kurome
International Journal of Systematic and Evolutionary Microbiology, 2007Co-Authors: Kae Kyoung Kwon, Sung Hyun YangAbstract:A marine bacterium, DOKDO 007T, was isolated from the rhizosphere of the marine alga Ecklonia kurome collected from Dokdo Island, Korea, in October 2004. The strain produced orange-coloured colonies on marine agar 2216. 16S rRNA gene sequence analysis indicated that the novel isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarities with members of the genus Muricauda (92.0–94.0 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genera Muricauda and Costertonia. Cells were aerobic, Gram-negative rods producing non-diffusible carotenoid pigments. In contrast to all other members of the family Flavobacteriaceae, cells of DOKDO 007T were motile by means of a polar flagellum. Optimal growth occurred in the presence of 3.5–4 % (w/v) sea salts (corresponding to 2.7–3.1 % NaCl), at pH 8 and at temperatures of 26–29 °C. The novel strain required Ca2+ ions in addition to NaCl for growth. The dominant fatty acids were iso-15 : 0, iso-15 : 1ω10c and 10-methyl-16 : 0. The major respiratory quinone was MK-6. The DNA G+C content was 56.3 mol%, an unusually high value for members of the family Flavobacteriaceae. On the basis of these polyphasic taxonomic data, strain DOKDO 007T should be classified as representing a new genus and novel species in the family Flavobacteriaceae, for which the name Flagellimonas Eckloniae gen. nov., sp. nov. is proposed. The type strain is DOKDO 007T (=KCCM 42307T=JCM 13831T).
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Flagellimonas Eckloniae gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from the rhizosphere of Ecklonia kurome.
International journal of systematic and evolutionary microbiology, 2007Co-Authors: Seung Seob Bae, Kae Kyoung Kwon, Sung Hyun Yang, Hee-soon Lee, Sang-jin Kim, Jung-hyun LeeAbstract:A marine bacterium, DOKDO 007(T), was isolated from the rhizosphere of the marine alga Ecklonia kurome collected from Dokdo Island, Korea, in October 2004. The strain produced orange-coloured colonies on marine agar 2216. 16S rRNA gene sequence analysis indicated that the novel isolate belonged to the family Flavobacteriaceae and showed relatively high sequence similarities with members of the genus Muricauda (92.0-94.0 %). Phylogenetic analysis based on nearly complete 16S rRNA gene sequences revealed that the novel isolate shared a lineage with members of the genera Muricauda and Costertonia. Cells were aerobic, Gram-negative rods producing non-diffusible carotenoid pigments. In contrast to all other members of the family Flavobacteriaceae, cells of DOKDO 007(T) were motile by means of a polar flagellum. Optimal growth occurred in the presence of 3.5-4 % (w/v) sea salts (corresponding to 2.7-3.1 % NaCl), at pH 8 and at temperatures of 26-29 degrees C. The novel strain required Ca(2+) ions in addition to NaCl for growth. The dominant fatty acids were iso-15 : 0, iso-15 : 1omega10c and 10-methyl-16 : 0. The major respiratory quinone was MK-6. The DNA G+C content was 56.3 mol%, an unusually high value for members of the family Flavobacteriaceae. On the basis of these polyphasic taxonomic data, strain DOKDO 007(T) should be classified as representing a new genus and novel species in the family Flavobacteriaceae, for which the name Flagellimonas Eckloniae gen. nov., sp. nov. is proposed. The type strain is DOKDO 007(T) (=KCCM 42307(T)=JCM 13831(T)).
