The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Peter D White - One of the best experts on this subject based on the ideXlab platform.
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a solid phase linker strategy for the direct synthesis of EDANS labelled peptide substrates
Tetrahedron Letters, 2005Co-Authors: Joerg Beythien, Peter D WhiteAbstract:A novel linker strategy for the efficient synthesis of peptides C-terminally labelled with the EDANS fluorophore is described. Using this support, FRET peptide substrates bearing EDANS/Dabcyl fluorescent donor/acceptor groups can be readily prepared using standard Fmoc solid phase methods.
Joerg Beythien - One of the best experts on this subject based on the ideXlab platform.
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a solid phase linker strategy for the direct synthesis of EDANS labelled peptide substrates
Tetrahedron Letters, 2005Co-Authors: Joerg Beythien, Peter D WhiteAbstract:A novel linker strategy for the efficient synthesis of peptides C-terminally labelled with the EDANS fluorophore is described. Using this support, FRET peptide substrates bearing EDANS/Dabcyl fluorescent donor/acceptor groups can be readily prepared using standard Fmoc solid phase methods.
Antonello Pessi - One of the best experts on this subject based on the ideXlab platform.
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an improved solid phase synthesis of resonance energy transfer fluorescent peptides and depsipeptides employing the EDANS dabcyl donor acceptor pair
Letters in Peptide Science, 1997Co-Authors: Marina Taliani, Elisabetta Bianchi, Antonello PessiAbstract:Fluorescent peptide substrates of the resonance energy transfer type, employing thedonor–acceptor pair 5-[(2′-aminoethyl)amino]naphthalene sulphonic acid(EDANS) and 4-[[4′-(dimethylamino)phenyl]azo]benzoic acid (DABCYL), are widelyused for continuous monitoring of the activity of various proteases. We describe here aflexible synthetic scheme which allows the synthesis of peptides having EDANS andDABCYL in any position of the sequence; the synthesis is entirely performed on solid phasewith standard methods and makes use only of EDANS, DABCYL and commercially availableamino acid derivatives. Moreover, we show that our scheme can be equally applied to thesynthesis of EDANS/DABCYL-derivatised depsipeptides.
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An improved solid-phase synthesis of resonance energy transfer fluorescent peptides and depsipeptides employing the EDANS/DABCYL donor-acceptor pair
Letters in Peptide Science, 1997Co-Authors: Marina Taliani, Elisabetta Bianchi, Antonello PessiAbstract:Fluorescent peptide substrates of the resonance energy transfer type, employing thedonor–acceptor pair 5-[(2′-aminoethyl)amino]naphthalene sulphonic acid(EDANS) and 4-[[4′-(dimethylamino)phenyl]azo]benzoic acid (DABCYL), are widelyused for continuous monitoring of the activity of various proteases. We describe here aflexible synthetic scheme which allows the synthesis of peptides having EDANS andDABCYL in any position of the sequence; the synthesis is entirely performed on solid phasewith standard methods and makes use only of EDANS, DABCYL and commercially availableamino acid derivatives. Moreover, we show that our scheme can be equally applied to thesynthesis of EDANS/DABCYL-derivatised depsipeptides.
Susana P G Costa - One of the best experts on this subject based on the ideXlab platform.
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intermolecular quenching of EDANS dabcyl donor acceptor fret pair
2019Co-Authors: Catia D F Martins, Manuela M M Raposo, Susana P G CostaAbstract:The intermolecular quenching between 5-(2′-aminoethyl)aminonaphthalene sulfonic acid (EDANS) and 4-[[4′-(N,N-dimethylamino)phenyl]diazenyl]benzoic acid (Dabcyl) was studied by photometric and fluorimetric measurements at pH 7.5 in phosphate buffer. The spectral properties of the EDANS/Dabcyl donor–acceptor pair were determined and Dabcyl exhibited an intense absorption band at 463 nm, contributing to the quenching efficiency. We also found the primary requirement for fluorescence resonance energy transfer (FRET), the excellent overlap between the fluorescence emission spectrum of the donor molecule and the absorption spectrum of the acceptor molecule, resulting in efficient energy transfer. The quenching mechanism was studied using the Stern–Volmer plot, confirming that this FRET pair was involved in a dynamic quenching process.
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Intermolecular quenching of EDANS/Dabcyl Donor–Acceptor FRET pair
Proceedings, 2019Co-Authors: Catia D F Martins, Manuela M M Raposo, Susana P G CostaAbstract:The intermolecular quenching between 5-(2′-aminoethyl)aminonaphthalene sulfonic acid (EDANS) and 4-[[4′-(N,N-dimethylamino)phenyl]diazenyl]benzoic acid (Dabcyl) was studied by photometric and fluorimetric measurements at pH 7.5 in phosphate buffer. The spectral properties of the EDANS/Dabcyl donor–acceptor pair were determined and Dabcyl exhibited an intense absorption band at 463 nm, contributing to the quenching efficiency. We also found the primary requirement for fluorescence resonance energy transfer (FRET), the excellent overlap between the fluorescence emission spectrum of the donor molecule and the absorption spectrum of the acceptor molecule, resulting in efficient energy transfer. The quenching mechanism was studied using the Stern–Volmer plot, confirming that this FRET pair was involved in a dynamic quenching process.
Marina Taliani - One of the best experts on this subject based on the ideXlab platform.
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an improved solid phase synthesis of resonance energy transfer fluorescent peptides and depsipeptides employing the EDANS dabcyl donor acceptor pair
Letters in Peptide Science, 1997Co-Authors: Marina Taliani, Elisabetta Bianchi, Antonello PessiAbstract:Fluorescent peptide substrates of the resonance energy transfer type, employing thedonor–acceptor pair 5-[(2′-aminoethyl)amino]naphthalene sulphonic acid(EDANS) and 4-[[4′-(dimethylamino)phenyl]azo]benzoic acid (DABCYL), are widelyused for continuous monitoring of the activity of various proteases. We describe here aflexible synthetic scheme which allows the synthesis of peptides having EDANS andDABCYL in any position of the sequence; the synthesis is entirely performed on solid phasewith standard methods and makes use only of EDANS, DABCYL and commercially availableamino acid derivatives. Moreover, we show that our scheme can be equally applied to thesynthesis of EDANS/DABCYL-derivatised depsipeptides.
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An improved solid-phase synthesis of resonance energy transfer fluorescent peptides and depsipeptides employing the EDANS/DABCYL donor-acceptor pair
Letters in Peptide Science, 1997Co-Authors: Marina Taliani, Elisabetta Bianchi, Antonello PessiAbstract:Fluorescent peptide substrates of the resonance energy transfer type, employing thedonor–acceptor pair 5-[(2′-aminoethyl)amino]naphthalene sulphonic acid(EDANS) and 4-[[4′-(dimethylamino)phenyl]azo]benzoic acid (DABCYL), are widelyused for continuous monitoring of the activity of various proteases. We describe here aflexible synthetic scheme which allows the synthesis of peptides having EDANS andDABCYL in any position of the sequence; the synthesis is entirely performed on solid phasewith standard methods and makes use only of EDANS, DABCYL and commercially availableamino acid derivatives. Moreover, we show that our scheme can be equally applied to thesynthesis of EDANS/DABCYL-derivatised depsipeptides.
