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Salida de campo a la laguna de La Nava (Fuentes de Nava, Palencia) los días 1, 2 y 3 de noviembre de 1952
2009Co-Authors: Valverde Gómez, José AntonioAbstract:Salida de campo a la laguna de La Nava, en Fuentes de Nava, recorriendo también Grijota y Villamartín de Campos, en la provincia de Palencia, los días 1, 2 y 3 de noviembre de 1952, de la que se anotaron observaciones sobre el pez Carassius sp. (Pez rojo), el reptil Natrix maura (Culebra viperina, llamada Tropidonotus viperinus por el autor), los siguientes anfibios: Pelobates cultripes (Sapo de espuelas) y Pelophylax esculentus (Rana verde europea, llamada Rana sculenta o R.esculenta ridibunda por el autor), los siguientes mamíferos: Apodemus sp. (Ratón), Dama dama (Gamo), Equus caballus (Caballo), Lepus sp. (Liebre), Mula, Ovis aries (Oveja doméstica) y "Pytimis" (llamado así por el autor, siendo posiblemente el actual género Microtus), y las siguientes aves: Acrocephalus sp. (Carricero), Actitis hypoleucos (Andarríos chico, llamado Actynioides hypoleucus por el autor), Alauda arvensis (Alondra común), Anas crecca (Cerceta común), Anas penelope (Silbón europeo), Anas platyrhynchos (Ánade azulón, también llamado Pato bravío por el autor), Anas querquedula (Cerceta carretona), Anser anser (Ánsar común, también llamado Anser sylvestris por el autor), Anser fabalis (Ánsar campestre), Anthus pratensis (Bisbita común), Anthus spinoletta (Bisbita alpino), Ardea cinerea (Garza real), Ardea purpurea (Garza imperial), Athene sp. (Mochuelo), Botaurus stellaris (Avetoro común), Calandrella sp. (Terrera), Chlidonias sp. (Fumarel), Circus aeruginosus (Aguilucho lagunero occidental), Circus cyaneus (Aguilucho pálido), Corvus corone (Corneja negra), Coturnix coturnix (Codorniz común), Falco tinnunculus (Cernícalo vulgar), Fulica sp. (Focha), Galerida cristata (Cogujada común), Galerida malabarica (Cogujada malabar), Gallinago gallinago (Agachadiza común, llamada Capella gallinago por el autor), Grus grus (Grulla común), Himantopus himantopus (Cigüeñuela común), Hirundo sp. (Golondrina), Larus ridibundus (Gaviota reidora), Lullula arborea (Totovía), Melanocorypha calandra (Calandria), Milvus milvus (Milano real), Motacilla alba (Lavandera blanca), Otis tarda (Avutarda común), Passer sp. (Gorrión), Phalaropus fulicarius (Falaporo picogrueso), Phoenicurus ochruros (Colirrojo tizón), Pluvialis apricaria (Chorlito Dorado Europeo, llamado Charadrius apricarius por el autor), Sturnus sp. (Estornino), Sturnus unicolor (Estornino negro), Tringa nebularia (Archibebe claro), Tringa ochropus (Andarríos grande), Turdus ericetorum (considerado actualmente Turdus philomelos, el Zorzal común), Turdus merula (Mirlo común), Turdus musicus (considerado actualmente Turdus philomelos, el Zorzal común), Tyto alba (Lechuza común) y Vanellus vanellus (Avefría europea). Se incluyen análisis de algunos contenidos estomacales, una descripción detallada sobre ecología de Grullas, y algunas pequeñas ilustraciones.Field trip to the lake of La Nava, at Fuentes de Nava, also travelling through Grijota and Villamartín de Campos, at the province of Palencia, the 1st, 2nd and 3rd of November of 1952, of which there were noted observations about the fish Carassius sp. (Goldfish), the reptil Natrix maura (Viperine Snake, refered as Tropidonotus viperinus by the author), , the following mammals: Apodemus sp. (Field mouse), Dama dama (Fallow Deer), Equus caballus (Horse), Lepus sp. (Hare), Mule, Ovis aries (Domestic Sheep), and "Pytimis" (as refered by the author, being possibly the current genus Microtus),the following amphibians: Pelobates cultripes (Western Spadefoot) and Pelophylax esculentus (Edible Frog, refered as Rana sculenta or R.esculenta ridibunda by the author), and the following birds: Acrocephalus sp. (Warbler), Actitis hypoleucos (Common Sandpiper, refered as Actynioides hypoleucus by the author), Alauda arvensis (Eurasian Skylark), Anas crecca (Common Teal), Anas penelope (Eurasian Wigeon), Anas platyrhynchos (Mallard), Anas querquedula (Garganey), Anser anser (Greylag Goose, also refered as Anser sylvestris by the author), Anser fabalis (Bean Goose), Anthus pratensis (Meadow Pipit), Anthus spinoletta (Water Pipit), Ardea cinerea (Grey Heron), Ardea purpurea (Purple Heron), Athene sp. (Owl), Botaurus stellaris (Great Bittern), Calandrella sp. (Lark), Chlidonias sp. (Tern), Circus aeruginosus (Western Marsh-Harrier), Circus cyaneus (Northern Harrier), Corvus corone (Carrion Crow), Coturnix coturnix (Common Quail), Falco tinnunculus (Common Kestrel), Fulica sp. (Coot), Galerida cristata (Crested Lark), Galerida malabarica (Malabar Lark), Gallinago gallinago (Common Snipe, refered as Capella gallinago by the author), Grus grus (Common Crane), Himantopus himantopus (Black-winged Stilt), Hirundo sp. (Swallow), Larus ridibundus (Common Black-headed Gull), Lullula arborea (Wood Lark), Melanocorypha calandra (Calandria Lark), Milvus milvus (Red Kite), Motacilla alba (White Wagtail), Otis tarda (Great Bustard), Passer sp. (Sparrow), Phalaropus fulicarius (Grey Phalapore), Phoenicurus ochruros (Black Redstart), Pluvialis apricaria (Eurasian Golden Plover, refered as Charadrius apricarius by the author), Sturnus sp. (Starling), Sturnus unicolor (Spotless Starling), Tringa nebularia (Common Greenshank), Tringa ochropus (Green Sandpiper), Turdus ericetorum (currently considered Turdus philomelos, the Song Thrush), Turdus merula (Eurasian Blackbird), Turdus musicus (currently considered Turdus philomelos, the Song Thrush), Tyto alba (Barn Owl) and Vanellus vanellus (Northern Lapwing). Analysis of some stomach contents, a detailed description about the ecology of Cranes, and some little illustrations are included
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Salida de campo a Laguna de Duero (Valladolid) el 19 de mayo de 1952
2009Co-Authors: Valverde Gómez, José AntonioAbstract:Salida de campo a Laguna de Duero, en Valladolid, el 19 de mayo de 1952, de la que se anotaron observaciones sobre anidamiento, cría y alimentación de Ciconia ciconia (Cigüeña blanca). Se analizaron los contenidos estomacales de tres pollos, en los que aparecieron, entre insectos y otros elementos, los siguientes herpetos: Natrix maura (Culebra viperina, llamada Tropidonotus viperinus por el autor), Pelophylax esculentus (Rana verde europea, llamada Rana sculenta o R.esculenta ridibunda por el autor) y Rana iberica (Rana patilarga). Además se incluye una pequeña ilustración a bolígrafo de una Cigüeña blanca.Field trip to Laguna de Duero, at Valladolid, the 19th of May of 1952, of which there were noted observations about nesting, breeging and feeding ecology of Ciconia ciconia (White Stork). Stomach contents of three juveniles were analyzed, containing, with insects and other components, the following herpetofauna: Natrix maura (Viperine Snake, refered as Tropidonotus viperinus by the author), Pelophylax esculentus (Edible Frog, refered as Rana sculenta or R.esculenta ridibunda by the author) and Rana iberica (Iberian Frog). A little pen illustration of a White Stork is also included
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Salida de campo a Laguna de Duero, Valdestillas y Villanubla, en Valladolid, el 24 de agosto de 1952
2009Co-Authors: Valverde Gómez, José AntonioAbstract:Salidas de campo simultáneas a Laguna de Duero, Valdestillas y Villanubla, en Valladolid, del autor, Mariano Sáiz Pardo y Luis Valverde Gómez, respectivamente, el 24 de agosto de 1952, de las que el autor anotó observaciones sobre los siguientes anfibios: ranas (sin concretar la especie), Pelophylax esculentus (Rana verde europea, llamada Rana sculenta o R.esculenta ridibunda por el autor) y Pleurodeles waltl (Gallipato, que en este caso puede referirse a una observación errónea, ya que el autor lo idenifica con salamanquesas), los siguientes reptiles: Malpolon monspessulanus (Culebra bastarda, llamada Coleopeltis mouspessulanus por el autor), Psammodromus sp. (referente al antiguo P.algirus), los siguientes mamíferos: Apodemus sp. (Ratón), Eptesicus serotinus (Murciélago hortelano), Lepus sp. (Liebre ibérica), Psammodromus sp. y las siguientes aves: Anthus campestris (Bisbita campestre), Apus apus (Vencejo común), Calandrella sp. (Terrera), Caprimulgus sp. (Chotacabras), Carduelis cannabina (Pardillo común, llamada Acanthis cannabina por el autor), Carduelis chloris (Verderón común, llamado Chloris chloris por el autor), Certhia sp. (Agateador, también conocido como Chapin), Ciconia ciconia (Cigüeña blanca), Circus pygargus (Aguilucho cenizo), Circus sp. (Aguilucho), Coracias garrulus (Carraca europea, también llamada Azulejo por el autor), Corvus frugilegus (Graja), Coturnix coturnix (Codorniz común), Cuculus canorus (Cuco común) , Cyanopica cooki (Rabilargo, llamado "Charro" y C. cyana por el autor), Delichon urbica (Avión común, llamado Chelidon urbica por el autor), Falco naumanni (Cernícalo primilla), Falco subbuteo (Alcotán), Falco tinnunculus (Cernícalo vulgar), Ficedula hypoleuca (Papamoscas cerrojillo, llamado Muscicapa hypoleuca por el autor), Fringilla sp. (Pinzón), Galerida sp. (Cogujada), Hippolais sp. (Zarcero), Lullula arborea (Totovía), Melanocorypha calandra (Calandria), Merops apiaster (Abejaruco europeo), Miliaria calandra (Triguero, llamada Emberiza calandra por el autor), Motacilla flava (Lavandera boyera), Oriolus oriolus (Oropéndola), Parus major (Carbonero común, también conocido como Chapin), Passer montanus (Gorrión molinero), Phylloscopus sp. (Mosquitero), Phylloscopus trochilus (Mosquitero musical), Pica pica (Urraca, llamada "marica" por el autor), Serinus serinus (Verdecillo), Streptopelia sp. (Tórtola), Sturnus unicolor (Estornino negro) y Upupa epops (Abubilla, también llamada Bubillo por el autor).Simoultaneus field trips to Laguna de Duero, Valdestillas and Villanubla, at Valladolid, carried out by the author, Mariano, Sáiz Pardo and Luis Valverde Gómez, respectively, the 24th of August of 1952, of which the author noted observations about the following amphibians: Frogs (without specifying the species), Pelophylax esculentus (Edible Frog, refered as Rana sculenta or R.esculenta ridibunda by the author) and Pleurodeles waltl (Iberian Ribbed Newt; that might be a wrong observation because the author identified it with a gecko), the following reptiles: Malpolon monspessulanus (Montpellier Snake, refered as Coleopeltis mouspessulanus by the author) and Psammodromus sp. (that it refered to the old P.algirus)the follwing mammals: Apodemus sp. (Field mouse), Eptesicus serotinus (Serotine Bat), Lepus sp. (Hare), Psammodromus sp. and the following birds: Anthus campestris (Tawny Pipit), Apus apus (Common Swift), Calandrella sp. (Lark), Caprimulgus sp. (Nightjar), Carduelis cannabina (Eurasian Linnet, refered as Acanthis cannabina by the author), Carduelis chloris (European Greenfinch, refered as Chloris chloris by the author), Certhia sp. (Tree-creeper), Ciconia ciconia (White Stork), Circus pygargus (Montagu´s Harrier), Circus sp. (Harrier), Coracias garrulus (European Roller), Corvus frugilegus (Rook), Coturnix coturnix (Common Quail), Cuculus canorus (Common Cuckoo), Cyanopica cooki (Azure-winged Magpie, refered as C. cyana by the author), Delichon urbica (House Martin, refered as Chelidon urbica by the author), Falco naumanni (Lesser Kestrel), Falco subbuteo (Eurasian Hobby), Falco tinnunculus (Common Kestrel), Ficedula hypoleuca (European Pied Flycatcher, refered as Muscicapa hypoleuca by the author), Fringilla sp. (Chaffinch), Galerida sp. (Lark), Hippolais sp. (Warbler), Melanocorypha calandra (Calandria Lark), Merops apiaster (European Bee-eater), Miliaria calandra (Corn Bunting, refered as Emberiza calandra by the author), Motacilla flava (Yellow Wagtail), Lullula arborea (Wood Lark), Oriolus oriolus (Eurasian Golden-oriole), Parus major (Great Tit), Passer montanus (Eurasian Tree Sparrow), Phylloscopus sp. (Common), Phylloscopus trochilus (Willow Warbler), Pica pica (Black-billed Magpie), Serinus serinus (European Serin), Streptopelia sp. (Turtle-dove), Sturnus unicolor (Spotless Starling) and Upupa epops (Eurasia Hoopoe)
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Salida de campo a la laguna de Gallocanta (Zaragoza) el 12 y 13 de julio de 1952
2009Co-Authors: Valverde Gómez, José AntonioAbstract:Salida de campo a la laguna de Gallocanta, en Zaragoza, del autor acompañado de Francisco Bernis, el 12 y 13 de julio de 1952, de la que se anotaron observaciones sobre el anfibio Pelophylax esculentus (Rana verde europea, llamada Rana sculenta o R.esculenta ridibunda por el autor), el reptil Natrix maura (Culebra viperina, llamada Tropidonotus viperinus por el autor), el mamífero Apodemus sylvaticus (Ratón de campo), y las siguientes aves: Acrocephalus arundinaceus (Carricero tordal), Actitis hypoleucos (Andarríos chico, llamado, Actynioides hypoleucus por el autor), Alauda arvensis (Alondra común), Anas crecca (Cerceta común), Anas platyrhynchos (Ánade azulón, también llamado Pato bravío por el autor), Anthus campestris (Bisbita campestre), Ardea cinerea (Garza real), Aythya ferina (Porrón europeo), Burhinus oedicnemus (Alcaraván común), Carduelis cannabina (Pardillo común, llamada Colorín y Acanthis cannabina por el autor), Calandrella sp. (Terrera), Carduelis sp. (seguramente, el Jilguero, C.carduelis), Charadrius alexandrinus (Chorlitejo patinegro), Chlidonias leucopterus (Fumarel aliblanco), Ciconia ciconia (Cigüeña blanca), Circus aeruginosus (Aguilucho lagunero occidental), Circus cyaneus (Aguilucho pálido), Corvus corone (Corneja negra), Cuculus canorus (Cuco común), Falco subbuteo (Alcotán), Falco tinnunculus (Cernícalo vulgar), Fulica sp. (Focha), Glareola pratincola (Canastera común), Gyps fulvus (Buitre leonado), Himantopus himantopus (Cigüeñuela común), Larus ridibundus (Gaviota reidora), Limosa limosa (Aguja colinegra), Numenius arquata (Zarapito real), Melanocorypha calandra (Calandria), Miliaria calandra (Triguero, llamada, Emberiza calandra por el autor), Motacilla cinerea (Lavandera cascadeña), Motacilla flava (Lavandera boyera), Oenanthe oenanthe (Collalba gris), Philomachus pugnax (Combatiente), Phylloscopus bonelli (Mosquitero papialbo), Phylloscopus trochilus (Mosquitero musical), Pica pica (Urraca, llamada "marica" y "picarza" por el autor), Pterocles orientalis (Ganga ortega), Rallus aquaticus (Rascón europeo), Tringa glareola (Andarríos bastardo), Tringa ochropus (Andarríos grande), Tringa totanus (Archibebe común) y Vencejo (Apus sp. o Tachymarptis melba). Se incluyen descripciones de vegetación y un esquema a bolígrafo de la laguna.Field trip to the lake of Gallocanta, at Zaragoza, of the author and Francisco Bernis, the 12th and 13th of July of 1952, of which there were noted observations about the amphibian Pelophylax esculentus (Edible Frog, refered as Rana sculenta or R.esculenta ridibunda by the author), the reptil Natrix maura (Viperine Snake, refered as Tropidonotus viperinus by the author), the mammal Arvicola sapidus (Southern Water Vole), and the following birds: Acrocephalus arundinaceus (Great Reed-warbler), Actitis hypoleucos (Common Sandpiper, refered as Actynioides hypoleucus by the author), Alauda arvensis (Eurasian Skylark), Anas crecca (Common Teal), Anas platyrhynchos (Mallard), Anthus campestris (Tawny Pipit), Ardea cinerea (Grey Heron), Aythya ferina (Common Pochard), Burhinus oedicnemus (Eurasian Thick-Knee), Calandrella sp. (Lark), Carduelis cannabina (Eurasian Linnet, refered as, Acanthis cannabina by the author), Carduelis sp. (probably, the European Goldfinch, C.Carduelis), Charadrius alexandrinus (Kentish Plover), Chlidonias leucopterus (White-winged Tern), Ciconia ciconia (White Stork), Circus aeruginosus (Western Marsh-Harrier), Circus cyaneus (Northern Harrier), Corvus corone (Carrion Crow), Cuculus canorus (Common Cuckoo), Falco subbuteo (Eurasian Hobby), Falco tinnunculus (Common Kestrel), Fulica sp. (Coot), Glareola pratincola (Collared Pratincole), Gyps fulvus (Eurasian Griffon), Himantopus himantopus (Black-winged Stilt), Larus ridibundus (Common Black-headed Gull), Limosa limosa (Black-tailed Godwit), Numenius arquata (Eurasian Curlew), Melanocorypha calandra (Calandria Lark), Miliaria calandra (Corn Bunting, refered as Emberiza calandra by the author), Motacilla cinerea (Grey Wagtail), Motacilla flava (Yellow Wagtail), Oenanthe oenanthe (Northern Wheatear), Philomachus pugnax (Ruff), Phylloscopus bonelli (Bonelli´s Warbler), Phylloscopus trochilus (Willow Warbler), Pica pica (Black-billed Magpie), Pterocles orientalis (Black-bellied Sandgrouse), Rallus aquaticus (Water Rail), Swift (Apus sp. or Tachymarptis melba), Tringa glareola (Wood Sandpiper), Tringa ochropus (Green Sandpiper) and Tringa totanus (Common Redshank). Description of the vegetation and a pen diagram of the lake are included
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Salida de campo a Laguna de Duero (Valladolid) el 31 de marzo de 1951
2009Co-Authors: Valverde Gómez, José AntonioAbstract:Salida de campo a Laguna de Duero (Valladolid) el 31 de marzo de 1951, de la que se anotaron observaciones sobre las siguientes aves: Acrocephalus sp. (Carricero), Alectoris rufa (Perdiz roja), Anas platyrhynchos (Ánade azulón), Anser anser (Ánsar común), Anthus spinoletta (Bisbita alpino), Ardea sp. (Garza), Carduelis cannabina (Pardillo común, llamada Acanthis cannabina por el autor), Ciconia ciconia (Cigüeña blanca), Circus aeruginosus (Aguilucho lagunero occidental), Clamator glandarius (Críalo europeo), Corvus corone (Corneja negra), Falco tinnunculus (Cernícalo vulgar), Fringilla sp. (Pinzón), Galerida cristata (Cogujada común), Gallinula chloropus (Gallineta común), Larus ridibundus (Gaviota reidora), Miliaria calandra (Triguero, llamada Emberiza calandra por el autor), Milvus migrans (Milano negro), Motacilla cinerea (Lavandera cascadeña), Motacilla flava (Lavandera boyera), Phoenicurus ochruros (Colirrojo tizón), Phoenicurus phoenicurus (Colirrojo real), Phylloscopus collybita (Mosquitero común), Phylloscopus trochilus (Mosquitero musical), Pica pica (Urraca), Saxicola torquata (Tarabilla común), Serinus serinus (Verdecillo), Sturnus unicolor (Estornino negro), Tringa ochropus (Andarríos grande), Tringa totanus (Archibebe común) and Vanellus vanellus (Avefría europea), los siguientes anfibios: Epidalea calamita (Sapo corredor, llamado Bufo calamita por el autor), Pelobates cultripes (Sapo de espuelas), Pelophylax esculentus (Rana verde europea, llamada Rana sculenta por el autor) y Pleurodeles waltl (Gallipato), y topillos (Pytimis sp.), además de analizarse la vegetación de la zona.Field trip to Laguna de Duero (Valladolid) the 31st of March of 1951, of which there were noted observations of the following birds: Acrocephalus sp. (Reed Warbler), Alectoris rufa (Red-leged Partridge), Anas platyrhynchos (Mallard), Anser anser (Greylag Goose), Anthus spinoletta (Water Pipit), Ardea sp. (Heron), Carduelis cannabina (Eurasian Linnet, refered as Acanthis cannabina by the author), Ciconia ciconia (White Stork), Circus aeruginosus (Western Marsh-Harrier), Clamator glandarius (Great Spotted Cuckoo), Corvus corone (Carrion Crow), Falco tinnunculus (Common Kestrel), Fringilla sp. (Chaffinch), Galerida cristata (Crested Lark), Gallinula chloropus (Common Moorhen), Larus ridibundus (Common Black-headed Gull), Miliaria calandra (Corn Bunting, refered as Emberiza calandra by the author), Milvus migrans (Black Kite), Motacilla cinerea (Grey Wagtail), Motacilla flava (Yellow Wagtail), Phoenicurus ochruros (Black Redstart), Phoenicurus phoenicurus (Common Redstart), Phylloscopus collybita (Common Chiffchaff), Phylloscopus trochilus (Willow Warbler), Pica pica (Black-billed Magpie), Saxicola torquata (African Stonechat), Serinus serinus (European Serin), Sturnus unicolor (Spotless Starling), Tringa ochropus (Green Sandpiper), Tringa totanus (Common Redshank) and Vanellus vanellus (Northern Lapwing), amphibians: Epidalea calamita (Natterjack Toad, refered as Bufo calamita by the author), Pelobates cultripes (Western Spadefoot), Pelophylax esculentus (Edible Frog, refered as Rana sculenta by the author) and Pleurodeles waltl (Iberian Ribbed Newt), and little moles (Pytimis sp.). The vegetation of the habitat was also analysed
Valverde Gómez - One of the best experts on this subject based on the ideXlab platform.
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Salida de campo a la laguna de La Nava (Fuentes de Nava, Palencia) los días 1, 2 y 3 de noviembre de 1952
2009Co-Authors: Valverde Gómez, José AntonioAbstract:Salida de campo a la laguna de La Nava, en Fuentes de Nava, recorriendo también Grijota y Villamartín de Campos, en la provincia de Palencia, los días 1, 2 y 3 de noviembre de 1952, de la que se anotaron observaciones sobre el pez Carassius sp. (Pez rojo), el reptil Natrix maura (Culebra viperina, llamada Tropidonotus viperinus por el autor), los siguientes anfibios: Pelobates cultripes (Sapo de espuelas) y Pelophylax esculentus (Rana verde europea, llamada Rana sculenta o R.esculenta ridibunda por el autor), los siguientes mamíferos: Apodemus sp. (Ratón), Dama dama (Gamo), Equus caballus (Caballo), Lepus sp. (Liebre), Mula, Ovis aries (Oveja doméstica) y "Pytimis" (llamado así por el autor, siendo posiblemente el actual género Microtus), y las siguientes aves: Acrocephalus sp. (Carricero), Actitis hypoleucos (Andarríos chico, llamado Actynioides hypoleucus por el autor), Alauda arvensis (Alondra común), Anas crecca (Cerceta común), Anas penelope (Silbón europeo), Anas platyrhynchos (Ánade azulón, también llamado Pato bravío por el autor), Anas querquedula (Cerceta carretona), Anser anser (Ánsar común, también llamado Anser sylvestris por el autor), Anser fabalis (Ánsar campestre), Anthus pratensis (Bisbita común), Anthus spinoletta (Bisbita alpino), Ardea cinerea (Garza real), Ardea purpurea (Garza imperial), Athene sp. (Mochuelo), Botaurus stellaris (Avetoro común), Calandrella sp. (Terrera), Chlidonias sp. (Fumarel), Circus aeruginosus (Aguilucho lagunero occidental), Circus cyaneus (Aguilucho pálido), Corvus corone (Corneja negra), Coturnix coturnix (Codorniz común), Falco tinnunculus (Cernícalo vulgar), Fulica sp. (Focha), Galerida cristata (Cogujada común), Galerida malabarica (Cogujada malabar), Gallinago gallinago (Agachadiza común, llamada Capella gallinago por el autor), Grus grus (Grulla común), Himantopus himantopus (Cigüeñuela común), Hirundo sp. (Golondrina), Larus ridibundus (Gaviota reidora), Lullula arborea (Totovía), Melanocorypha calandra (Calandria), Milvus milvus (Milano real), Motacilla alba (Lavandera blanca), Otis tarda (Avutarda común), Passer sp. (Gorrión), Phalaropus fulicarius (Falaporo picogrueso), Phoenicurus ochruros (Colirrojo tizón), Pluvialis apricaria (Chorlito Dorado Europeo, llamado Charadrius apricarius por el autor), Sturnus sp. (Estornino), Sturnus unicolor (Estornino negro), Tringa nebularia (Archibebe claro), Tringa ochropus (Andarríos grande), Turdus ericetorum (considerado actualmente Turdus philomelos, el Zorzal común), Turdus merula (Mirlo común), Turdus musicus (considerado actualmente Turdus philomelos, el Zorzal común), Tyto alba (Lechuza común) y Vanellus vanellus (Avefría europea). Se incluyen análisis de algunos contenidos estomacales, una descripción detallada sobre ecología de Grullas, y algunas pequeñas ilustraciones.Field trip to the lake of La Nava, at Fuentes de Nava, also travelling through Grijota and Villamartín de Campos, at the province of Palencia, the 1st, 2nd and 3rd of November of 1952, of which there were noted observations about the fish Carassius sp. (Goldfish), the reptil Natrix maura (Viperine Snake, refered as Tropidonotus viperinus by the author), , the following mammals: Apodemus sp. (Field mouse), Dama dama (Fallow Deer), Equus caballus (Horse), Lepus sp. (Hare), Mule, Ovis aries (Domestic Sheep), and "Pytimis" (as refered by the author, being possibly the current genus Microtus),the following amphibians: Pelobates cultripes (Western Spadefoot) and Pelophylax esculentus (Edible Frog, refered as Rana sculenta or R.esculenta ridibunda by the author), and the following birds: Acrocephalus sp. (Warbler), Actitis hypoleucos (Common Sandpiper, refered as Actynioides hypoleucus by the author), Alauda arvensis (Eurasian Skylark), Anas crecca (Common Teal), Anas penelope (Eurasian Wigeon), Anas platyrhynchos (Mallard), Anas querquedula (Garganey), Anser anser (Greylag Goose, also refered as Anser sylvestris by the author), Anser fabalis (Bean Goose), Anthus pratensis (Meadow Pipit), Anthus spinoletta (Water Pipit), Ardea cinerea (Grey Heron), Ardea purpurea (Purple Heron), Athene sp. (Owl), Botaurus stellaris (Great Bittern), Calandrella sp. (Lark), Chlidonias sp. (Tern), Circus aeruginosus (Western Marsh-Harrier), Circus cyaneus (Northern Harrier), Corvus corone (Carrion Crow), Coturnix coturnix (Common Quail), Falco tinnunculus (Common Kestrel), Fulica sp. (Coot), Galerida cristata (Crested Lark), Galerida malabarica (Malabar Lark), Gallinago gallinago (Common Snipe, refered as Capella gallinago by the author), Grus grus (Common Crane), Himantopus himantopus (Black-winged Stilt), Hirundo sp. (Swallow), Larus ridibundus (Common Black-headed Gull), Lullula arborea (Wood Lark), Melanocorypha calandra (Calandria Lark), Milvus milvus (Red Kite), Motacilla alba (White Wagtail), Otis tarda (Great Bustard), Passer sp. (Sparrow), Phalaropus fulicarius (Grey Phalapore), Phoenicurus ochruros (Black Redstart), Pluvialis apricaria (Eurasian Golden Plover, refered as Charadrius apricarius by the author), Sturnus sp. (Starling), Sturnus unicolor (Spotless Starling), Tringa nebularia (Common Greenshank), Tringa ochropus (Green Sandpiper), Turdus ericetorum (currently considered Turdus philomelos, the Song Thrush), Turdus merula (Eurasian Blackbird), Turdus musicus (currently considered Turdus philomelos, the Song Thrush), Tyto alba (Barn Owl) and Vanellus vanellus (Northern Lapwing). Analysis of some stomach contents, a detailed description about the ecology of Cranes, and some little illustrations are included
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Salida de campo a Laguna de Duero (Valladolid) el 19 de mayo de 1952
2009Co-Authors: Valverde Gómez, José AntonioAbstract:Salida de campo a Laguna de Duero, en Valladolid, el 19 de mayo de 1952, de la que se anotaron observaciones sobre anidamiento, cría y alimentación de Ciconia ciconia (Cigüeña blanca). Se analizaron los contenidos estomacales de tres pollos, en los que aparecieron, entre insectos y otros elementos, los siguientes herpetos: Natrix maura (Culebra viperina, llamada Tropidonotus viperinus por el autor), Pelophylax esculentus (Rana verde europea, llamada Rana sculenta o R.esculenta ridibunda por el autor) y Rana iberica (Rana patilarga). Además se incluye una pequeña ilustración a bolígrafo de una Cigüeña blanca.Field trip to Laguna de Duero, at Valladolid, the 19th of May of 1952, of which there were noted observations about nesting, breeging and feeding ecology of Ciconia ciconia (White Stork). Stomach contents of three juveniles were analyzed, containing, with insects and other components, the following herpetofauna: Natrix maura (Viperine Snake, refered as Tropidonotus viperinus by the author), Pelophylax esculentus (Edible Frog, refered as Rana sculenta or R.esculenta ridibunda by the author) and Rana iberica (Iberian Frog). A little pen illustration of a White Stork is also included
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Salida de campo a Laguna de Duero, Valdestillas y Villanubla, en Valladolid, el 24 de agosto de 1952
2009Co-Authors: Valverde Gómez, José AntonioAbstract:Salidas de campo simultáneas a Laguna de Duero, Valdestillas y Villanubla, en Valladolid, del autor, Mariano Sáiz Pardo y Luis Valverde Gómez, respectivamente, el 24 de agosto de 1952, de las que el autor anotó observaciones sobre los siguientes anfibios: ranas (sin concretar la especie), Pelophylax esculentus (Rana verde europea, llamada Rana sculenta o R.esculenta ridibunda por el autor) y Pleurodeles waltl (Gallipato, que en este caso puede referirse a una observación errónea, ya que el autor lo idenifica con salamanquesas), los siguientes reptiles: Malpolon monspessulanus (Culebra bastarda, llamada Coleopeltis mouspessulanus por el autor), Psammodromus sp. (referente al antiguo P.algirus), los siguientes mamíferos: Apodemus sp. (Ratón), Eptesicus serotinus (Murciélago hortelano), Lepus sp. (Liebre ibérica), Psammodromus sp. y las siguientes aves: Anthus campestris (Bisbita campestre), Apus apus (Vencejo común), Calandrella sp. (Terrera), Caprimulgus sp. (Chotacabras), Carduelis cannabina (Pardillo común, llamada Acanthis cannabina por el autor), Carduelis chloris (Verderón común, llamado Chloris chloris por el autor), Certhia sp. (Agateador, también conocido como Chapin), Ciconia ciconia (Cigüeña blanca), Circus pygargus (Aguilucho cenizo), Circus sp. (Aguilucho), Coracias garrulus (Carraca europea, también llamada Azulejo por el autor), Corvus frugilegus (Graja), Coturnix coturnix (Codorniz común), Cuculus canorus (Cuco común) , Cyanopica cooki (Rabilargo, llamado "Charro" y C. cyana por el autor), Delichon urbica (Avión común, llamado Chelidon urbica por el autor), Falco naumanni (Cernícalo primilla), Falco subbuteo (Alcotán), Falco tinnunculus (Cernícalo vulgar), Ficedula hypoleuca (Papamoscas cerrojillo, llamado Muscicapa hypoleuca por el autor), Fringilla sp. (Pinzón), Galerida sp. (Cogujada), Hippolais sp. (Zarcero), Lullula arborea (Totovía), Melanocorypha calandra (Calandria), Merops apiaster (Abejaruco europeo), Miliaria calandra (Triguero, llamada Emberiza calandra por el autor), Motacilla flava (Lavandera boyera), Oriolus oriolus (Oropéndola), Parus major (Carbonero común, también conocido como Chapin), Passer montanus (Gorrión molinero), Phylloscopus sp. (Mosquitero), Phylloscopus trochilus (Mosquitero musical), Pica pica (Urraca, llamada "marica" por el autor), Serinus serinus (Verdecillo), Streptopelia sp. (Tórtola), Sturnus unicolor (Estornino negro) y Upupa epops (Abubilla, también llamada Bubillo por el autor).Simoultaneus field trips to Laguna de Duero, Valdestillas and Villanubla, at Valladolid, carried out by the author, Mariano, Sáiz Pardo and Luis Valverde Gómez, respectively, the 24th of August of 1952, of which the author noted observations about the following amphibians: Frogs (without specifying the species), Pelophylax esculentus (Edible Frog, refered as Rana sculenta or R.esculenta ridibunda by the author) and Pleurodeles waltl (Iberian Ribbed Newt; that might be a wrong observation because the author identified it with a gecko), the following reptiles: Malpolon monspessulanus (Montpellier Snake, refered as Coleopeltis mouspessulanus by the author) and Psammodromus sp. (that it refered to the old P.algirus)the follwing mammals: Apodemus sp. (Field mouse), Eptesicus serotinus (Serotine Bat), Lepus sp. (Hare), Psammodromus sp. and the following birds: Anthus campestris (Tawny Pipit), Apus apus (Common Swift), Calandrella sp. (Lark), Caprimulgus sp. (Nightjar), Carduelis cannabina (Eurasian Linnet, refered as Acanthis cannabina by the author), Carduelis chloris (European Greenfinch, refered as Chloris chloris by the author), Certhia sp. (Tree-creeper), Ciconia ciconia (White Stork), Circus pygargus (Montagu´s Harrier), Circus sp. (Harrier), Coracias garrulus (European Roller), Corvus frugilegus (Rook), Coturnix coturnix (Common Quail), Cuculus canorus (Common Cuckoo), Cyanopica cooki (Azure-winged Magpie, refered as C. cyana by the author), Delichon urbica (House Martin, refered as Chelidon urbica by the author), Falco naumanni (Lesser Kestrel), Falco subbuteo (Eurasian Hobby), Falco tinnunculus (Common Kestrel), Ficedula hypoleuca (European Pied Flycatcher, refered as Muscicapa hypoleuca by the author), Fringilla sp. (Chaffinch), Galerida sp. (Lark), Hippolais sp. (Warbler), Melanocorypha calandra (Calandria Lark), Merops apiaster (European Bee-eater), Miliaria calandra (Corn Bunting, refered as Emberiza calandra by the author), Motacilla flava (Yellow Wagtail), Lullula arborea (Wood Lark), Oriolus oriolus (Eurasian Golden-oriole), Parus major (Great Tit), Passer montanus (Eurasian Tree Sparrow), Phylloscopus sp. (Common), Phylloscopus trochilus (Willow Warbler), Pica pica (Black-billed Magpie), Serinus serinus (European Serin), Streptopelia sp. (Turtle-dove), Sturnus unicolor (Spotless Starling) and Upupa epops (Eurasia Hoopoe)
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Salida de campo a la laguna de Gallocanta (Zaragoza) el 12 y 13 de julio de 1952
2009Co-Authors: Valverde Gómez, José AntonioAbstract:Salida de campo a la laguna de Gallocanta, en Zaragoza, del autor acompañado de Francisco Bernis, el 12 y 13 de julio de 1952, de la que se anotaron observaciones sobre el anfibio Pelophylax esculentus (Rana verde europea, llamada Rana sculenta o R.esculenta ridibunda por el autor), el reptil Natrix maura (Culebra viperina, llamada Tropidonotus viperinus por el autor), el mamífero Apodemus sylvaticus (Ratón de campo), y las siguientes aves: Acrocephalus arundinaceus (Carricero tordal), Actitis hypoleucos (Andarríos chico, llamado, Actynioides hypoleucus por el autor), Alauda arvensis (Alondra común), Anas crecca (Cerceta común), Anas platyrhynchos (Ánade azulón, también llamado Pato bravío por el autor), Anthus campestris (Bisbita campestre), Ardea cinerea (Garza real), Aythya ferina (Porrón europeo), Burhinus oedicnemus (Alcaraván común), Carduelis cannabina (Pardillo común, llamada Colorín y Acanthis cannabina por el autor), Calandrella sp. (Terrera), Carduelis sp. (seguramente, el Jilguero, C.carduelis), Charadrius alexandrinus (Chorlitejo patinegro), Chlidonias leucopterus (Fumarel aliblanco), Ciconia ciconia (Cigüeña blanca), Circus aeruginosus (Aguilucho lagunero occidental), Circus cyaneus (Aguilucho pálido), Corvus corone (Corneja negra), Cuculus canorus (Cuco común), Falco subbuteo (Alcotán), Falco tinnunculus (Cernícalo vulgar), Fulica sp. (Focha), Glareola pratincola (Canastera común), Gyps fulvus (Buitre leonado), Himantopus himantopus (Cigüeñuela común), Larus ridibundus (Gaviota reidora), Limosa limosa (Aguja colinegra), Numenius arquata (Zarapito real), Melanocorypha calandra (Calandria), Miliaria calandra (Triguero, llamada, Emberiza calandra por el autor), Motacilla cinerea (Lavandera cascadeña), Motacilla flava (Lavandera boyera), Oenanthe oenanthe (Collalba gris), Philomachus pugnax (Combatiente), Phylloscopus bonelli (Mosquitero papialbo), Phylloscopus trochilus (Mosquitero musical), Pica pica (Urraca, llamada "marica" y "picarza" por el autor), Pterocles orientalis (Ganga ortega), Rallus aquaticus (Rascón europeo), Tringa glareola (Andarríos bastardo), Tringa ochropus (Andarríos grande), Tringa totanus (Archibebe común) y Vencejo (Apus sp. o Tachymarptis melba). Se incluyen descripciones de vegetación y un esquema a bolígrafo de la laguna.Field trip to the lake of Gallocanta, at Zaragoza, of the author and Francisco Bernis, the 12th and 13th of July of 1952, of which there were noted observations about the amphibian Pelophylax esculentus (Edible Frog, refered as Rana sculenta or R.esculenta ridibunda by the author), the reptil Natrix maura (Viperine Snake, refered as Tropidonotus viperinus by the author), the mammal Arvicola sapidus (Southern Water Vole), and the following birds: Acrocephalus arundinaceus (Great Reed-warbler), Actitis hypoleucos (Common Sandpiper, refered as Actynioides hypoleucus by the author), Alauda arvensis (Eurasian Skylark), Anas crecca (Common Teal), Anas platyrhynchos (Mallard), Anthus campestris (Tawny Pipit), Ardea cinerea (Grey Heron), Aythya ferina (Common Pochard), Burhinus oedicnemus (Eurasian Thick-Knee), Calandrella sp. (Lark), Carduelis cannabina (Eurasian Linnet, refered as, Acanthis cannabina by the author), Carduelis sp. (probably, the European Goldfinch, C.Carduelis), Charadrius alexandrinus (Kentish Plover), Chlidonias leucopterus (White-winged Tern), Ciconia ciconia (White Stork), Circus aeruginosus (Western Marsh-Harrier), Circus cyaneus (Northern Harrier), Corvus corone (Carrion Crow), Cuculus canorus (Common Cuckoo), Falco subbuteo (Eurasian Hobby), Falco tinnunculus (Common Kestrel), Fulica sp. (Coot), Glareola pratincola (Collared Pratincole), Gyps fulvus (Eurasian Griffon), Himantopus himantopus (Black-winged Stilt), Larus ridibundus (Common Black-headed Gull), Limosa limosa (Black-tailed Godwit), Numenius arquata (Eurasian Curlew), Melanocorypha calandra (Calandria Lark), Miliaria calandra (Corn Bunting, refered as Emberiza calandra by the author), Motacilla cinerea (Grey Wagtail), Motacilla flava (Yellow Wagtail), Oenanthe oenanthe (Northern Wheatear), Philomachus pugnax (Ruff), Phylloscopus bonelli (Bonelli´s Warbler), Phylloscopus trochilus (Willow Warbler), Pica pica (Black-billed Magpie), Pterocles orientalis (Black-bellied Sandgrouse), Rallus aquaticus (Water Rail), Swift (Apus sp. or Tachymarptis melba), Tringa glareola (Wood Sandpiper), Tringa ochropus (Green Sandpiper) and Tringa totanus (Common Redshank). Description of the vegetation and a pen diagram of the lake are included
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Salida de campo a Laguna de Duero (Valladolid) el 31 de marzo de 1951
2009Co-Authors: Valverde Gómez, José AntonioAbstract:Salida de campo a Laguna de Duero (Valladolid) el 31 de marzo de 1951, de la que se anotaron observaciones sobre las siguientes aves: Acrocephalus sp. (Carricero), Alectoris rufa (Perdiz roja), Anas platyrhynchos (Ánade azulón), Anser anser (Ánsar común), Anthus spinoletta (Bisbita alpino), Ardea sp. (Garza), Carduelis cannabina (Pardillo común, llamada Acanthis cannabina por el autor), Ciconia ciconia (Cigüeña blanca), Circus aeruginosus (Aguilucho lagunero occidental), Clamator glandarius (Críalo europeo), Corvus corone (Corneja negra), Falco tinnunculus (Cernícalo vulgar), Fringilla sp. (Pinzón), Galerida cristata (Cogujada común), Gallinula chloropus (Gallineta común), Larus ridibundus (Gaviota reidora), Miliaria calandra (Triguero, llamada Emberiza calandra por el autor), Milvus migrans (Milano negro), Motacilla cinerea (Lavandera cascadeña), Motacilla flava (Lavandera boyera), Phoenicurus ochruros (Colirrojo tizón), Phoenicurus phoenicurus (Colirrojo real), Phylloscopus collybita (Mosquitero común), Phylloscopus trochilus (Mosquitero musical), Pica pica (Urraca), Saxicola torquata (Tarabilla común), Serinus serinus (Verdecillo), Sturnus unicolor (Estornino negro), Tringa ochropus (Andarríos grande), Tringa totanus (Archibebe común) and Vanellus vanellus (Avefría europea), los siguientes anfibios: Epidalea calamita (Sapo corredor, llamado Bufo calamita por el autor), Pelobates cultripes (Sapo de espuelas), Pelophylax esculentus (Rana verde europea, llamada Rana sculenta por el autor) y Pleurodeles waltl (Gallipato), y topillos (Pytimis sp.), además de analizarse la vegetación de la zona.Field trip to Laguna de Duero (Valladolid) the 31st of March of 1951, of which there were noted observations of the following birds: Acrocephalus sp. (Reed Warbler), Alectoris rufa (Red-leged Partridge), Anas platyrhynchos (Mallard), Anser anser (Greylag Goose), Anthus spinoletta (Water Pipit), Ardea sp. (Heron), Carduelis cannabina (Eurasian Linnet, refered as Acanthis cannabina by the author), Ciconia ciconia (White Stork), Circus aeruginosus (Western Marsh-Harrier), Clamator glandarius (Great Spotted Cuckoo), Corvus corone (Carrion Crow), Falco tinnunculus (Common Kestrel), Fringilla sp. (Chaffinch), Galerida cristata (Crested Lark), Gallinula chloropus (Common Moorhen), Larus ridibundus (Common Black-headed Gull), Miliaria calandra (Corn Bunting, refered as Emberiza calandra by the author), Milvus migrans (Black Kite), Motacilla cinerea (Grey Wagtail), Motacilla flava (Yellow Wagtail), Phoenicurus ochruros (Black Redstart), Phoenicurus phoenicurus (Common Redstart), Phylloscopus collybita (Common Chiffchaff), Phylloscopus trochilus (Willow Warbler), Pica pica (Black-billed Magpie), Saxicola torquata (African Stonechat), Serinus serinus (European Serin), Sturnus unicolor (Spotless Starling), Tringa ochropus (Green Sandpiper), Tringa totanus (Common Redshank) and Vanellus vanellus (Northern Lapwing), amphibians: Epidalea calamita (Natterjack Toad, refered as Bufo calamita by the author), Pelobates cultripes (Western Spadefoot), Pelophylax esculentus (Edible Frog, refered as Rana sculenta by the author) and Pleurodeles waltl (Iberian Ribbed Newt), and little moles (Pytimis sp.). The vegetation of the habitat was also analysed
C. Shaw - One of the best experts on this subject based on the ideXlab platform.
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identification and bioactivity evaluation of two novel temporins from the skin secretion of the european Edible Frog pelophylax kl esculentus
2016Co-Authors: Xiaole Chen, Mei Zhou, Tianbao Chen, Lei Wang, He Wang, Mu Yang, C. ShawAbstract:The amphibian temporins, amongst the smallest antimicrobial peptides (AMPs), are α-helical, amphipathic, hydrophobic and cationic and are active mainly against Gram-positive bacteria but inactive or weakly active against Gram-negative bacteria. Here, we report two novel members of the temporin family, named temporin-1Ee (FLPVIAGVLSKLFamide) and temporin-1Re (FLPGLLAGLLamide), whose biosynthetic precursor structures were deduced from clones obtained from skin secretion-derived cDNA libraries of the European Edible Frog, Pelophylax kl. esculentus, by 'shotgun' cloning. Deduction of the molecular masses of each mature processed peptide from respective cloned cDNAs was used to locate respective molecules in reverse-phase HPLC fractions of secretion. Temporin-1Ee (MIC = 10 μM) and temporin-1Re (MIC = 60 μM) were both found to be active against Gram-positive Staphylococcus aureus, but retaining a weak haemolytic activity. To our knowledge, Single-site substitutions can dramatically change the spectrum of activity of a given temporin. Compared with temporine-1Ec, just one chemically-conservative substitution (Val8 instead of Leu8), temporin-1Ee bearing a net charge of +2 displays broad-spectrum activity with particularly high potency on the clinically relevant Gram-negative strains, Escherichia coli (MIC = 40 μM). These factors bode well for translating temporins to be potential drug candidates for the design of new and valuable anti-infective agents.
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identification and bioactivity evaluation of two novel temporins from the skin secretion of the european Edible Frog pelophylax kl esculentus
2016Co-Authors: Xiaole Chen, Mei Zhou, Tianbao Chen, Lei Wang, He Wang, Mu Yang, C. ShawAbstract:The amphibian temporins, amongst the smallest antimicrobial peptides (AMPS), are alpha-helical, amphipathic, hydrophobic and cationic and are active mainly against Gram-positive bacteria but inactive or weakly active against Gram-negative bacteria. Here, we report two novel members of the temporin family, named temporin-1Ee (FLINIAGVLSKLF(amide)) and temporin-1 Re (FLPGLLAGLL(amide)), whose biosynthetic precursor structures were deduced from clones obtained from skin secretion-derived cDNA libraries of the European Edible Frog, Pelophylax kl. esculentus, by 'shotgun' cloning. Deduction of the molecular masses of each mature processed peptide from respective cloned cDNAs was used to locate respective molecules in reverse-phase HPLC fractions of secretion. Temporin-1Ee (MIC = 10 mu M) and temporin-1Re (MIC = 60 mu M) were both found to be active against Gram-positive Staphylococcus aureus, but retaining a weak haemolytic activity. To our knowledge, Single-site substitutions can dramatically change the spectrum of activity of a given temporin. Compared with temporine-1Ec, just one chemically-conservative substitution (Val8 instead of Leu8), temporin-1 Ee bearing a net charge of +2 displays broad-spectrum activity with particularly high potency on the clinically relevant Gram-negative strains, Escherichia coli (MIC = 40 mu M). These factors bode well for translating temporins to be potential drug candidates for the design of new and valuable anti-infective agents. (C) 2016 Elsevier Inc. All rights reserved.
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identification of miscellaneous peptides from the skin secretion of the european Edible Frog pelophylax kl esculentus
2016Co-Authors: Xiaole Chen, Mei Zhou, Tianbao Chen, Lei Wang, He Wang, C. ShawAbstract:The chemical compounds synthesised and secreted from the dermal glands of amphibian have diverse bioactivities that play key roles in the hosts' innate immune system and in causing diverse pharmacological effects in predators that may ingest the defensive skin secretions. As new biotechnological methods have developed, increasing numbers of novel peptides with novel activities have been discovered from this source of natural compounds. In this study, a number of defensive skin secretion peptide sequences were obtained from the European Edible Frog, P. kl. esculentus, using a 'shotgun' cloning technique developed previously within our laboratory. Some of these sequences have been previously reported but had either obtained from other species or were isolated using different methods. Two new skin peptides are described here for the first time. Esculentin-2c and Brevinin-2Tbe belong to the Esculentin-2 and Brevinin-2 families, respectively, and both are very similar to their respective analogues but with a few amino acid differences. Further, [Asn-3, Lys-6, Phe-13] 3-14-bombesin isolated previously from the skin of the marsh Frog, Rana ridibunda, was identified here in the skin of P. kl. esculentus. Studies such as this can provide a rapid elucidation of peptide and corresponding DNA sequences from unstudied species of Frogs and can rapidly provide a basis for related scientific studies such as those involved in systematic or the evolution of a large diverse gene family and usage by biomedical researchers as a source of potential novel drug leads or pharmacological agents.
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a fish bradykinin arg0 trp5 leu8 bradykinin from the defensive skin secretion of the european Edible Frog pelophylax kl esculentus structural characterization molecular cloning of skin kininogen cdna and pharmacological effects on mammalian smooth mu
2011Co-Authors: Xiaole Chen, Mei Zhou, Tianbao Chen, Hang Chen, He Wang, C. ShawAbstract:Abstract Extensive studies on bradykinin-related peptides (BRPs) generated from plasma kininogens in representative species of various vertebrate taxa, have confirmed that many amphibian skin BRPs reflect those present in putative vertebrate predators. For example, the (Val 1 , Thr 6 )-bradykinin, present in the defensive skin secretions of many ranids and phyllomedusines, can be generated from plasma kininogens in colubrid snakes—common predators of these Frogs. Here, we report the presence of (Arg 0 , Trp 5 , Leu 8 )-bradykinin in the skin secretion of the European Edible Frog, Pelophylax kl . esculentus , and have found it to be encoded in single copy by a kininogen with an open-reading frame of 68 amino acid residues. This peptide is the archetypal bony fish bradykinin that has been generated from plasma kininogens of the bowfin ( Amia calva ), the long-nosed gar ( Lepisosteus oseus ) and the rainbow trout ( Onchorhynchus mykiss ). More recently, this peptide has been shown to be encoded within cloned kininogens of the Atlantic cod ( Gadus morhua ) spotted wolf-fish ( Anarichas minor ), zebrafish ( Danio rerio ), pufferfish ( Tetraodon nigroviridis ) and Northern pike ( Esox lucius ). The latter species is regarded as a major predator of P. kl. esculentus . Synthetic (Arg 0 , Trp 5 , Leu 8 )-bradykinin was previously reported as having multiphasic effects on arterial blood pressure in conscious trout and here we have demonstrated that it can antagonize the relaxation in rat arterial smooth muscle induced by canonical mammalian bradykinin. The discovery of (Arg 0 , Trp 5 , Leu 8 )-bradykinin in the defensive skin secretion of this amphibian completes the spectrum of vertebrate taxon-specific BRPs identified from this source.
Tianbao Chen - One of the best experts on this subject based on the ideXlab platform.
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identification and target modifications of temporin pe a novel antimicrobial peptide in the defensive skin secretions of the Edible Frog pelophylax kl esculentus
2018Co-Authors: Mengru Sang, James F. Burrows, Qinan Wu, Xinping Xi, Mei Zhou, Lei Wang, Tianbao ChenAbstract:Abstract A potent natural antimicrobial peptide named temporin-PE was identified and encoded from the skin secretions of Pelophylax kl. esculentus via “shotgun” cloning and LC-MS/MS fragmentation analysis. Target-modifications were carried out to further enhance the antimicrobial and anti-proliferative bioactivities, whilst decreasing the hemolytic effect. A range of bioassays demonstrated that replacing a proline with a tyrosine residue resulted in a loss of the bioactivity against Gram-negative bacteria, but dramatically improved the hemolytic and anti-proliferative activity, indicating the FLP- motif influences the hemolytic activity of temporins. Moreover, the coupling of TAT to the peptide dramatically improved its antimicrobial activity, indicating coupling TAT to these peptides could be considered as a potential tool to improve their antimicrobial activity. Overall, we have shown that targeted modifications of this natural antimicrobial peptide can adjust its bioactivities to help its development as an antibiotic or anti-proliferative agent.
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Pharmacological Effects of Two Novel Bombesin-Like Peptides from the Skin Secretions of Chinese Piebald Odorous Frog (Odorrana schmackeri) and European Edible Frog (Pelophylax kl. esculentus) on Smooth Muscle
2017Co-Authors: Xiaowei Zhou, Mei Zhou, Tianbao Chen, Yuning Zhang, Ruimin Zhong, Christopher Shaw, Lei WangAbstract:Bombesin-like peptides, which were identified from a diversity of amphibian skin secretions, have been demonstrated to possess several biological functions such as stimulation of smooth muscle contraction and regulation of food intake. Here, we report two novel bombesin-like peptides, bombesin-OS and bombesin-PE, which were isolated from Odorrana schmackeri and Pelophylax kl. esculentus, respectively. The mature peptides were identified and structurally confirmed by high performance Scliquid chromatography (HPLC) and tandem mass spectrometry (MS/MS). Subsequently, the effects of these purified chemically-synthetic peptides on smooth muscle were determined in bladder, uterus, and ileum. The synthetic replications were revealed to have significant pharmacological effects on these tissues. The EC50 values of bombesin-OS for bladder, uterus and ileum, were 10.8 nM, 33.64 nM, and 12.29 nM, respectively. Furthermore, compared with bombesin-OS, bombesin-PE showed similar contractile activity on ileum smooth muscle and uterus smooth muscle, but had a higher potency on bladder smooth muscle. The EC50 value of bombesin-OS for bladder was around 1000-fold less than that of bombesin-PE. This suggests that bombesin-OS and bombesin-PE have unique binding properties to their receptors. The precursor of bombesin-OS was homologous with that of a bombesin-like peptide, odorranain-BLP-5, and bombesin-PE belongs to the ranatensin subfamily. We identified the structure of bombesin-OS and bombesin-PE, two homologues peptides whose actions may provide a further clue in the classification of ranid Frogs, also in the provision of new drugs for human health.
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identification and bioactivity evaluation of two novel temporins from the skin secretion of the european Edible Frog pelophylax kl esculentus
2016Co-Authors: Xiaole Chen, Mei Zhou, Tianbao Chen, Lei Wang, He Wang, Mu Yang, C. ShawAbstract:The amphibian temporins, amongst the smallest antimicrobial peptides (AMPs), are α-helical, amphipathic, hydrophobic and cationic and are active mainly against Gram-positive bacteria but inactive or weakly active against Gram-negative bacteria. Here, we report two novel members of the temporin family, named temporin-1Ee (FLPVIAGVLSKLFamide) and temporin-1Re (FLPGLLAGLLamide), whose biosynthetic precursor structures were deduced from clones obtained from skin secretion-derived cDNA libraries of the European Edible Frog, Pelophylax kl. esculentus, by 'shotgun' cloning. Deduction of the molecular masses of each mature processed peptide from respective cloned cDNAs was used to locate respective molecules in reverse-phase HPLC fractions of secretion. Temporin-1Ee (MIC = 10 μM) and temporin-1Re (MIC = 60 μM) were both found to be active against Gram-positive Staphylococcus aureus, but retaining a weak haemolytic activity. To our knowledge, Single-site substitutions can dramatically change the spectrum of activity of a given temporin. Compared with temporine-1Ec, just one chemically-conservative substitution (Val8 instead of Leu8), temporin-1Ee bearing a net charge of +2 displays broad-spectrum activity with particularly high potency on the clinically relevant Gram-negative strains, Escherichia coli (MIC = 40 μM). These factors bode well for translating temporins to be potential drug candidates for the design of new and valuable anti-infective agents.
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identification and bioactivity evaluation of two novel temporins from the skin secretion of the european Edible Frog pelophylax kl esculentus
2016Co-Authors: Xiaole Chen, Mei Zhou, Tianbao Chen, Lei Wang, He Wang, Mu Yang, C. ShawAbstract:The amphibian temporins, amongst the smallest antimicrobial peptides (AMPS), are alpha-helical, amphipathic, hydrophobic and cationic and are active mainly against Gram-positive bacteria but inactive or weakly active against Gram-negative bacteria. Here, we report two novel members of the temporin family, named temporin-1Ee (FLINIAGVLSKLF(amide)) and temporin-1 Re (FLPGLLAGLL(amide)), whose biosynthetic precursor structures were deduced from clones obtained from skin secretion-derived cDNA libraries of the European Edible Frog, Pelophylax kl. esculentus, by 'shotgun' cloning. Deduction of the molecular masses of each mature processed peptide from respective cloned cDNAs was used to locate respective molecules in reverse-phase HPLC fractions of secretion. Temporin-1Ee (MIC = 10 mu M) and temporin-1Re (MIC = 60 mu M) were both found to be active against Gram-positive Staphylococcus aureus, but retaining a weak haemolytic activity. To our knowledge, Single-site substitutions can dramatically change the spectrum of activity of a given temporin. Compared with temporine-1Ec, just one chemically-conservative substitution (Val8 instead of Leu8), temporin-1 Ee bearing a net charge of +2 displays broad-spectrum activity with particularly high potency on the clinically relevant Gram-negative strains, Escherichia coli (MIC = 40 mu M). These factors bode well for translating temporins to be potential drug candidates for the design of new and valuable anti-infective agents. (C) 2016 Elsevier Inc. All rights reserved.
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identification of miscellaneous peptides from the skin secretion of the european Edible Frog pelophylax kl esculentus
2016Co-Authors: Xiaole Chen, Mei Zhou, Tianbao Chen, Lei Wang, He Wang, C. ShawAbstract:The chemical compounds synthesised and secreted from the dermal glands of amphibian have diverse bioactivities that play key roles in the hosts' innate immune system and in causing diverse pharmacological effects in predators that may ingest the defensive skin secretions. As new biotechnological methods have developed, increasing numbers of novel peptides with novel activities have been discovered from this source of natural compounds. In this study, a number of defensive skin secretion peptide sequences were obtained from the European Edible Frog, P. kl. esculentus, using a 'shotgun' cloning technique developed previously within our laboratory. Some of these sequences have been previously reported but had either obtained from other species or were isolated using different methods. Two new skin peptides are described here for the first time. Esculentin-2c and Brevinin-2Tbe belong to the Esculentin-2 and Brevinin-2 families, respectively, and both are very similar to their respective analogues but with a few amino acid differences. Further, [Asn-3, Lys-6, Phe-13] 3-14-bombesin isolated previously from the skin of the marsh Frog, Rana ridibunda, was identified here in the skin of P. kl. esculentus. Studies such as this can provide a rapid elucidation of peptide and corresponding DNA sequences from unstudied species of Frogs and can rapidly provide a basis for related scientific studies such as those involved in systematic or the evolution of a large diverse gene family and usage by biomedical researchers as a source of potential novel drug leads or pharmacological agents.
Xiaole Chen - One of the best experts on this subject based on the ideXlab platform.
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identification and bioactivity evaluation of two novel temporins from the skin secretion of the european Edible Frog pelophylax kl esculentus
2016Co-Authors: Xiaole Chen, Mei Zhou, Tianbao Chen, Lei Wang, He Wang, Mu Yang, C. ShawAbstract:The amphibian temporins, amongst the smallest antimicrobial peptides (AMPs), are α-helical, amphipathic, hydrophobic and cationic and are active mainly against Gram-positive bacteria but inactive or weakly active against Gram-negative bacteria. Here, we report two novel members of the temporin family, named temporin-1Ee (FLPVIAGVLSKLFamide) and temporin-1Re (FLPGLLAGLLamide), whose biosynthetic precursor structures were deduced from clones obtained from skin secretion-derived cDNA libraries of the European Edible Frog, Pelophylax kl. esculentus, by 'shotgun' cloning. Deduction of the molecular masses of each mature processed peptide from respective cloned cDNAs was used to locate respective molecules in reverse-phase HPLC fractions of secretion. Temporin-1Ee (MIC = 10 μM) and temporin-1Re (MIC = 60 μM) were both found to be active against Gram-positive Staphylococcus aureus, but retaining a weak haemolytic activity. To our knowledge, Single-site substitutions can dramatically change the spectrum of activity of a given temporin. Compared with temporine-1Ec, just one chemically-conservative substitution (Val8 instead of Leu8), temporin-1Ee bearing a net charge of +2 displays broad-spectrum activity with particularly high potency on the clinically relevant Gram-negative strains, Escherichia coli (MIC = 40 μM). These factors bode well for translating temporins to be potential drug candidates for the design of new and valuable anti-infective agents.
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identification and bioactivity evaluation of two novel temporins from the skin secretion of the european Edible Frog pelophylax kl esculentus
2016Co-Authors: Xiaole Chen, Mei Zhou, Tianbao Chen, Lei Wang, He Wang, Mu Yang, C. ShawAbstract:The amphibian temporins, amongst the smallest antimicrobial peptides (AMPS), are alpha-helical, amphipathic, hydrophobic and cationic and are active mainly against Gram-positive bacteria but inactive or weakly active against Gram-negative bacteria. Here, we report two novel members of the temporin family, named temporin-1Ee (FLINIAGVLSKLF(amide)) and temporin-1 Re (FLPGLLAGLL(amide)), whose biosynthetic precursor structures were deduced from clones obtained from skin secretion-derived cDNA libraries of the European Edible Frog, Pelophylax kl. esculentus, by 'shotgun' cloning. Deduction of the molecular masses of each mature processed peptide from respective cloned cDNAs was used to locate respective molecules in reverse-phase HPLC fractions of secretion. Temporin-1Ee (MIC = 10 mu M) and temporin-1Re (MIC = 60 mu M) were both found to be active against Gram-positive Staphylococcus aureus, but retaining a weak haemolytic activity. To our knowledge, Single-site substitutions can dramatically change the spectrum of activity of a given temporin. Compared with temporine-1Ec, just one chemically-conservative substitution (Val8 instead of Leu8), temporin-1 Ee bearing a net charge of +2 displays broad-spectrum activity with particularly high potency on the clinically relevant Gram-negative strains, Escherichia coli (MIC = 40 mu M). These factors bode well for translating temporins to be potential drug candidates for the design of new and valuable anti-infective agents. (C) 2016 Elsevier Inc. All rights reserved.
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identification of miscellaneous peptides from the skin secretion of the european Edible Frog pelophylax kl esculentus
2016Co-Authors: Xiaole Chen, Mei Zhou, Tianbao Chen, Lei Wang, He Wang, C. ShawAbstract:The chemical compounds synthesised and secreted from the dermal glands of amphibian have diverse bioactivities that play key roles in the hosts' innate immune system and in causing diverse pharmacological effects in predators that may ingest the defensive skin secretions. As new biotechnological methods have developed, increasing numbers of novel peptides with novel activities have been discovered from this source of natural compounds. In this study, a number of defensive skin secretion peptide sequences were obtained from the European Edible Frog, P. kl. esculentus, using a 'shotgun' cloning technique developed previously within our laboratory. Some of these sequences have been previously reported but had either obtained from other species or were isolated using different methods. Two new skin peptides are described here for the first time. Esculentin-2c and Brevinin-2Tbe belong to the Esculentin-2 and Brevinin-2 families, respectively, and both are very similar to their respective analogues but with a few amino acid differences. Further, [Asn-3, Lys-6, Phe-13] 3-14-bombesin isolated previously from the skin of the marsh Frog, Rana ridibunda, was identified here in the skin of P. kl. esculentus. Studies such as this can provide a rapid elucidation of peptide and corresponding DNA sequences from unstudied species of Frogs and can rapidly provide a basis for related scientific studies such as those involved in systematic or the evolution of a large diverse gene family and usage by biomedical researchers as a source of potential novel drug leads or pharmacological agents.
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a fish bradykinin arg0 trp5 leu8 bradykinin from the defensive skin secretion of the european Edible Frog pelophylax kl esculentus structural characterization molecular cloning of skin kininogen cdna and pharmacological effects on mammalian smooth mu
2011Co-Authors: Xiaole Chen, Mei Zhou, Tianbao Chen, Hang Chen, He Wang, C. ShawAbstract:Abstract Extensive studies on bradykinin-related peptides (BRPs) generated from plasma kininogens in representative species of various vertebrate taxa, have confirmed that many amphibian skin BRPs reflect those present in putative vertebrate predators. For example, the (Val 1 , Thr 6 )-bradykinin, present in the defensive skin secretions of many ranids and phyllomedusines, can be generated from plasma kininogens in colubrid snakes—common predators of these Frogs. Here, we report the presence of (Arg 0 , Trp 5 , Leu 8 )-bradykinin in the skin secretion of the European Edible Frog, Pelophylax kl . esculentus , and have found it to be encoded in single copy by a kininogen with an open-reading frame of 68 amino acid residues. This peptide is the archetypal bony fish bradykinin that has been generated from plasma kininogens of the bowfin ( Amia calva ), the long-nosed gar ( Lepisosteus oseus ) and the rainbow trout ( Onchorhynchus mykiss ). More recently, this peptide has been shown to be encoded within cloned kininogens of the Atlantic cod ( Gadus morhua ) spotted wolf-fish ( Anarichas minor ), zebrafish ( Danio rerio ), pufferfish ( Tetraodon nigroviridis ) and Northern pike ( Esox lucius ). The latter species is regarded as a major predator of P. kl. esculentus . Synthetic (Arg 0 , Trp 5 , Leu 8 )-bradykinin was previously reported as having multiphasic effects on arterial blood pressure in conscious trout and here we have demonstrated that it can antagonize the relaxation in rat arterial smooth muscle induced by canonical mammalian bradykinin. The discovery of (Arg 0 , Trp 5 , Leu 8 )-bradykinin in the defensive skin secretion of this amphibian completes the spectrum of vertebrate taxon-specific BRPs identified from this source.
