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Pilar Bermejobarrera - One of the best experts on this subject based on the ideXlab platform.
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ultrasound assisted enzymatic hydrolysis for iodinated amino acid extraction from Edible Seaweed before reversed phase high performance liquid chromatography inductively coupled plasma mass spectrometry
Journal of Chromatography A, 2013Co-Authors: Vanessa Romarishortas, Pilar Bermejobarrera, Antonio MoredapineiroAbstract:The combination of reverse phase high performance liquid chromatography (RP-HPLC) with inductively coupled plasma mass spectrometry (ICP-MS) was used for the determination of monoiodotyrosine (MIT) and diiodotyrosine (DIT) in Edible Seaweed. A sample pre-treatment based on ultrasound assisted enzymatic hydrolysis was optimized for the extraction of these iodinated amino acids. Pancreatin was selected as the most adequate type of enzyme, and parameters affecting the extraction efficiency (pH, temperature, mass of enzyme and extraction time) were evaluated by univariate approaches. In addition, extractable inorganic iodine (iodide) was also quantified by anion exchange high performance liquid chromatography (AE-HPLC) coupled with ICP-MS. The proposed procedure offered limits of detection of 1.1 and 4.3ngg(-1) for MIT and DIT, respectively. Total iodine contents in Seaweed, as well as total iodine in enzymatic digests were measured by ICP-MS after microwave assisted alkaline digestion with tetramethylamonium hydroxide (TMAH) for total iodine assessment, and also by treating the pancreatin extracts (extractable total iodine assessment). The optimized procedure was successfully applied to five different types of Edible Seaweed. The highest total iodine content, and also the highest iodide levels, was found in the brown Seaweed Kombu (6646±45μgg(-1)). Regarding iodinated amino acids, Nori (a red Seaweed) was by far the one with the highest amount of both species (42±3 and 0.41±0.024μgg(-1) for MIT and DIT, respectively). In general, MIT concentrations were much higher than the amounts of DIT, which suggests that iodine from iodinated proteins in Seaweed is most likely bound in the form of MIT residues.
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study of cooking on the bioavailability of as co cr cu fe ni se and zn from Edible Seaweed
Microchemical Journal, 2013Co-Authors: Cristina Garciasartal, Antonio Moredapineiro, Maria Carmen Barcielaalonso, Pilar BermejobarreraAbstract:Abstract This study examines the effects of cooking on the bioavailability of As, Co, Cr, Cu, Fe, Ni, Se and Zn from Edible Seaweed. Four Edible Galician Seaweeds (Kombu, Wakame, Sea Lettuce and Nori) were cooked following the manufacturer's recommendations. Cr, Fe, Co, Ni, Cu, Zn, As and Se were analyzed in raw and cooked Seaweeds after subjecting them to an acid digestion. The metals were released into the cooking water during the heat treatment with the exception of Ni and Zn in Kombu, Cr, Fe and Co in Wakame, and Zn in Nori, for which almost 100% was retained in the Seaweed. An in vitro digestion method was developed to assess the bioavailability percentages of those metals in the cooked Seaweed. Results suggested that Fe, Zn and Cu are not bioavailable for body functions in the studied matrices. Low dialyzability percentages were found for Cr, Co, Ni, As and Se, ranging from 1.0–16.0%, 3.4–27%, 8.2–12.9%, 9.7–21.0%, and 14.6–61.3%, respectively. Kombu Seaweed was used as a model to perform a mass balance study for evaluating the accuracy of the bioavailability study of cooked Seaweed. Standard deviation homogeneity was corroborated by means of the Cochran's and Bartlett's statistical tests (p-values > 0.05). An ANOVA test was used to compare the mean metal concentrations between and within groups. p-Values higher than 0.05 showed that there were no statistical differences, and the mass balance was adequate. Finally, the influence of the main food constituents (fat, protein, dietary fiber and carbohydrate) on the metal bioavailability was evaluated. Correlation matrix and multivariate analysis based on multiple linear regression indicated positive and negative correlations between the type of Seaweed and metal concentration.
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speciation of the bio available iodine and bromine forms in Edible Seaweed by high performance liquid chromatography hyphenated with inductively coupled plasma mass spectrometry
Analytica Chimica Acta, 2012Co-Authors: Vanessa Romarishortas, Pilar Bermejobarrera, Jorge Moredapineiro, Antonio MoredapineiroAbstract:A bioavailability study based on an in vitro dialyzability approach has been applied to assess the bio-available fractions of iodine and bromine species from Edible Seaweed. Iodide, iodate, 3-iodo-tyrosine (MIT), 3,5-diiodo-tyrosine (DIT), bromide and bromate were separated by anion exchange chromatography under a gradient elution mode (175 mM ammonium nitrate plus 15% (v/v) methanol, pH 3.8, as a mobile phase, and flow rates within the 0.5-1.5 mL min(-1) range). Inductively coupled plasma-mass spectrometry (ICP-MS) was used as a selective detector for iodine ((127)I) and bromine ((79)Br). Low dialyzability ratios (within the 2.0-18% range) were found for iodine species; whereas, moderate dialyzability percentages (from 9.0 to 40%) were obtained for bromine species. Iodide and bromide were the major species found in the dialyzates from Seaweed, although MIT and bromate were also found in the dialyzates from most of the Seaweed samples analysed. However, DIT was only found in dialyzates from Wakame, Kombu, and NIES 09 (Sargasso) certified reference material; whereas, iodate was not found in any dialyzate. Iodine dialyzability was found to be dependent on the protein content (negative correlation), and on the carbohydrate and dietary fibre levels (positive correlation). However, bromine dialyzability was only dependent on the protein amount in Seaweed (negative correlation).
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development of anion exchange reversed phase high performance liquid chromatography inductively coupled plasma mass spectrometry methods for the speciation of bio available iodine and bromine from Edible Seaweed
Journal of Chromatography A, 2012Co-Authors: Vanessa Romarishortas, Pilar Bermejobarrera, Antonio MoredapineiroAbstract:Abstract Anion exchange high performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry has been novelly applied to assess inorganic (iodide and iodate) and organic (3-iodotyrosine – MIT, and 3,5-diiodotyrosine – DIT) iodine species in a single chromatographic run. The optimized operating conditions (Dionex IonPac AS7, gradient elution with 175 mM ammonium nitrate plus 15% (v/v) methanol, pH 3.8, as a mobile phase and flow rates within the 0.5–1.5 mL min−1 range) have also been used to perform inorganic bromine speciation analysis (bromide and bromate). The developed method has been applied for determining the bio-available contents of iodine and bromine species in dialyzates from Edible Seaweed. Reverse phase high performance liquid chromatography (Zorbax Eclipse XDB-C8, gradient elution with 0.2% (m/m) acetic acid, and 0.2% (m/m) acetic acid in methanol, as mobile phases, and a constant flow rate of 0.75 mL min−1) also hyphenated with inductively coupled plasma-mass spectrometry was used to confirm the presence of organic iodine species (MIT and DIT) in the dialyzates. The verification of the presence of iodinated amino acids (MIT and DIT) in the extracts was also performed by reverse phase high performance liquid chromatography–electrospray ionization-mass spectrometry (LTQ Orbitrap). The developed methods have provided good repeatability (RSD values lower than 10% for both anion exchange and reverse phase separations) and analytical recoveries within the 90–105% range for all cases. The in vitro bio-availability method consisted of a simulated gastric and an intestinal digestion/dialysis (10 kDa molecular weight cut-off – MWCO) two-stage procedure. Iodide and MIT were the main bio-available species quantified, whereas bromide was the major bromine species found in the extracts.
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two dimensional hplc coupled to icp ms and electrospray ionisation esi ms ms for investigating the bioavailability in vitro of arsenic species from Edible Seaweed
Analytical and Bioanalytical Chemistry, 2012Co-Authors: Cristina Garciasartal, Pilar Bermejobarrera, Sutthinun Taebunpakul, Emma Stokes, M C Barcielaalonso, Heidi GoenagainfanteAbstract:Edible Seaweed consumption is a route of exposure to arsenic. However, little attention has been paid to estimate the bioaccessibility and/or bioavailability of arsenosugars in Edible Seaweed and their possible degradation products during gastrointestinal digestion. This work presents first use of combined inductively coupled plasma mass spectroscopy (ICP-MS) with electrospray ionization tandem mass spectrometry (ESI-MS/MS) with two-dimensional HPLC (size exclusion followed by anion exchange) to compare the qualitative and quantitative arsenosugars speciation of different Edible Seaweed with that of their bioavailable fraction as obtained using an in vitro gastrointestinal digestion procedure. Optimal extraction conditions for As species from four Seaweed namely kombu, wakame, nori and sea lettuce were selected as a compromise between As extraction efficiency and preservation of compound identity. For most investigated samples, the use of ammonium acetate buffer as extractant and 1 h sonication in a water bath followed by HPLC-ICP-MS resulted in 40-61% of the total As to be found in the buffered aqueous extract, of which 86-110% was present as arsenosugars (glycerol sugar, phosphate sugar and sulfonate sugar for wakame and kombu and glycerol sugar and phosphate sugar for nori). The exception was sea lettuce, for which the arsenosugar fraction (glycerol sugar, phosphate sugar) only comprised 44% of the total extracted As. Interestingly, the ratio of arsenobetaine and dimethylarsinic acid to arsenosugars in sea lettuce extracts seemed higher than that for the rest of investigated samples. After in vitro gastrointestinal digestion, approximately 11-16% of the total As in the solid sample was found in the dialyzates with arsenosugars comprising 93-120% and 41% of the dialyzable As fraction for kombu, wakame, nori and sea lettuce, respectively. Moreover, the relative As species distribution in Seaweed-buffered extracts and dialyzates was found to be very similar. Collection of specific fractions from the size-exclusion column to be analysed using anion-exchange HPLC-ESI-MS/MS enabled improved chromatographic selectivity, particularly for the less retained arsenosugar (glycerol sugar), facilitating confirmation of the presence of arsenosugars in Seaweed extracts and dialyzates. Using this approach, the presence of arsenobetaine in sea lettuce samples was also confirmed.
Antonio Moredapineiro - One of the best experts on this subject based on the ideXlab platform.
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ultrasound assisted enzymatic hydrolysis for iodinated amino acid extraction from Edible Seaweed before reversed phase high performance liquid chromatography inductively coupled plasma mass spectrometry
Journal of Chromatography A, 2013Co-Authors: Vanessa Romarishortas, Pilar Bermejobarrera, Antonio MoredapineiroAbstract:The combination of reverse phase high performance liquid chromatography (RP-HPLC) with inductively coupled plasma mass spectrometry (ICP-MS) was used for the determination of monoiodotyrosine (MIT) and diiodotyrosine (DIT) in Edible Seaweed. A sample pre-treatment based on ultrasound assisted enzymatic hydrolysis was optimized for the extraction of these iodinated amino acids. Pancreatin was selected as the most adequate type of enzyme, and parameters affecting the extraction efficiency (pH, temperature, mass of enzyme and extraction time) were evaluated by univariate approaches. In addition, extractable inorganic iodine (iodide) was also quantified by anion exchange high performance liquid chromatography (AE-HPLC) coupled with ICP-MS. The proposed procedure offered limits of detection of 1.1 and 4.3ngg(-1) for MIT and DIT, respectively. Total iodine contents in Seaweed, as well as total iodine in enzymatic digests were measured by ICP-MS after microwave assisted alkaline digestion with tetramethylamonium hydroxide (TMAH) for total iodine assessment, and also by treating the pancreatin extracts (extractable total iodine assessment). The optimized procedure was successfully applied to five different types of Edible Seaweed. The highest total iodine content, and also the highest iodide levels, was found in the brown Seaweed Kombu (6646±45μgg(-1)). Regarding iodinated amino acids, Nori (a red Seaweed) was by far the one with the highest amount of both species (42±3 and 0.41±0.024μgg(-1) for MIT and DIT, respectively). In general, MIT concentrations were much higher than the amounts of DIT, which suggests that iodine from iodinated proteins in Seaweed is most likely bound in the form of MIT residues.
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study of cooking on the bioavailability of as co cr cu fe ni se and zn from Edible Seaweed
Microchemical Journal, 2013Co-Authors: Cristina Garciasartal, Antonio Moredapineiro, Maria Carmen Barcielaalonso, Pilar BermejobarreraAbstract:Abstract This study examines the effects of cooking on the bioavailability of As, Co, Cr, Cu, Fe, Ni, Se and Zn from Edible Seaweed. Four Edible Galician Seaweeds (Kombu, Wakame, Sea Lettuce and Nori) were cooked following the manufacturer's recommendations. Cr, Fe, Co, Ni, Cu, Zn, As and Se were analyzed in raw and cooked Seaweeds after subjecting them to an acid digestion. The metals were released into the cooking water during the heat treatment with the exception of Ni and Zn in Kombu, Cr, Fe and Co in Wakame, and Zn in Nori, for which almost 100% was retained in the Seaweed. An in vitro digestion method was developed to assess the bioavailability percentages of those metals in the cooked Seaweed. Results suggested that Fe, Zn and Cu are not bioavailable for body functions in the studied matrices. Low dialyzability percentages were found for Cr, Co, Ni, As and Se, ranging from 1.0–16.0%, 3.4–27%, 8.2–12.9%, 9.7–21.0%, and 14.6–61.3%, respectively. Kombu Seaweed was used as a model to perform a mass balance study for evaluating the accuracy of the bioavailability study of cooked Seaweed. Standard deviation homogeneity was corroborated by means of the Cochran's and Bartlett's statistical tests (p-values > 0.05). An ANOVA test was used to compare the mean metal concentrations between and within groups. p-Values higher than 0.05 showed that there were no statistical differences, and the mass balance was adequate. Finally, the influence of the main food constituents (fat, protein, dietary fiber and carbohydrate) on the metal bioavailability was evaluated. Correlation matrix and multivariate analysis based on multiple linear regression indicated positive and negative correlations between the type of Seaweed and metal concentration.
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speciation of the bio available iodine and bromine forms in Edible Seaweed by high performance liquid chromatography hyphenated with inductively coupled plasma mass spectrometry
Analytica Chimica Acta, 2012Co-Authors: Vanessa Romarishortas, Pilar Bermejobarrera, Jorge Moredapineiro, Antonio MoredapineiroAbstract:A bioavailability study based on an in vitro dialyzability approach has been applied to assess the bio-available fractions of iodine and bromine species from Edible Seaweed. Iodide, iodate, 3-iodo-tyrosine (MIT), 3,5-diiodo-tyrosine (DIT), bromide and bromate were separated by anion exchange chromatography under a gradient elution mode (175 mM ammonium nitrate plus 15% (v/v) methanol, pH 3.8, as a mobile phase, and flow rates within the 0.5-1.5 mL min(-1) range). Inductively coupled plasma-mass spectrometry (ICP-MS) was used as a selective detector for iodine ((127)I) and bromine ((79)Br). Low dialyzability ratios (within the 2.0-18% range) were found for iodine species; whereas, moderate dialyzability percentages (from 9.0 to 40%) were obtained for bromine species. Iodide and bromide were the major species found in the dialyzates from Seaweed, although MIT and bromate were also found in the dialyzates from most of the Seaweed samples analysed. However, DIT was only found in dialyzates from Wakame, Kombu, and NIES 09 (Sargasso) certified reference material; whereas, iodate was not found in any dialyzate. Iodine dialyzability was found to be dependent on the protein content (negative correlation), and on the carbohydrate and dietary fibre levels (positive correlation). However, bromine dialyzability was only dependent on the protein amount in Seaweed (negative correlation).
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development of anion exchange reversed phase high performance liquid chromatography inductively coupled plasma mass spectrometry methods for the speciation of bio available iodine and bromine from Edible Seaweed
Journal of Chromatography A, 2012Co-Authors: Vanessa Romarishortas, Pilar Bermejobarrera, Antonio MoredapineiroAbstract:Abstract Anion exchange high performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry has been novelly applied to assess inorganic (iodide and iodate) and organic (3-iodotyrosine – MIT, and 3,5-diiodotyrosine – DIT) iodine species in a single chromatographic run. The optimized operating conditions (Dionex IonPac AS7, gradient elution with 175 mM ammonium nitrate plus 15% (v/v) methanol, pH 3.8, as a mobile phase and flow rates within the 0.5–1.5 mL min−1 range) have also been used to perform inorganic bromine speciation analysis (bromide and bromate). The developed method has been applied for determining the bio-available contents of iodine and bromine species in dialyzates from Edible Seaweed. Reverse phase high performance liquid chromatography (Zorbax Eclipse XDB-C8, gradient elution with 0.2% (m/m) acetic acid, and 0.2% (m/m) acetic acid in methanol, as mobile phases, and a constant flow rate of 0.75 mL min−1) also hyphenated with inductively coupled plasma-mass spectrometry was used to confirm the presence of organic iodine species (MIT and DIT) in the dialyzates. The verification of the presence of iodinated amino acids (MIT and DIT) in the extracts was also performed by reverse phase high performance liquid chromatography–electrospray ionization-mass spectrometry (LTQ Orbitrap). The developed methods have provided good repeatability (RSD values lower than 10% for both anion exchange and reverse phase separations) and analytical recoveries within the 90–105% range for all cases. The in vitro bio-availability method consisted of a simulated gastric and an intestinal digestion/dialysis (10 kDa molecular weight cut-off – MWCO) two-stage procedure. Iodide and MIT were the main bio-available species quantified, whereas bromide was the major bromine species found in the extracts.
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bioavailability study using an in vitro method of iodine and bromine in Edible Seaweed
Food Chemistry, 2011Co-Authors: Vanessa Romaris Hortas, Raquel Dominguezgonzalez, Antonio Moredapineiro, Cristina Garciasartal, Maria Carmen Barcielaalonso, Pilar BermejobarreraAbstract:Abstract Raw Edible Seaweed harvested in the Galician coast (Northwester Spain), including two red Seaweed types (Dulse and Nori), three brown Seaweed (Kombu, Wakame and Sea Spaghetti), one green Seaweed (Sea Lettuce) and one microalgae ( Spirulina platensis ) were analysed for total iodine and total bromine, as well as for iodine and bromine bioavailability by in-vitro methods (simulated gastric and intestinal digestion/dialysis). Similarly, a cooked Seaweed sample (canned in brine) consisting of a mixture of two brown Seaweed (Sea Spaghetti and Furbelows) and a derived product (agar–agar) from the red Seaweed Gelidiumm sesquipedale , were also included in the study. All measurements were carried out by inductively coupled plasma–mass spectrometry using tellurium and yttrium as internal standards for iodine and bromine, respectively. An optimised microwave assisted alkaline (TMAH) digestion procedure was used as sample pre-treatment for total iodine and bromine determinations, as well as for the determination of both elements in the non-dialyzable fractions. PIPES buffer solution at a pH of 7.0 and dialysis membranes of 10 kDa molecular weight cut off (MWCO) were used for the intestinal digestion. Accuracy of the method (total bromine and iodine determinations) was assessed by analysing a NIES-09 certified reference material. The accuracy of the in-vitro procedure was established by a mass-balance study which led, after statistical evaluation (95% confidence interval), good accuracy of the whole in-vitro process. The highest dialyzability bromine percentages (36 ± 0.7% and 47 ± 3.0%) were obtained for red Seaweed (Dulse and Nori), while higher dialyzability iodine was assessed for the brown Seaweed (Kombu), around 17% ± 0.7%.
Vanessa Romarishortas - One of the best experts on this subject based on the ideXlab platform.
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ultrasound assisted enzymatic hydrolysis for iodinated amino acid extraction from Edible Seaweed before reversed phase high performance liquid chromatography inductively coupled plasma mass spectrometry
Journal of Chromatography A, 2013Co-Authors: Vanessa Romarishortas, Pilar Bermejobarrera, Antonio MoredapineiroAbstract:The combination of reverse phase high performance liquid chromatography (RP-HPLC) with inductively coupled plasma mass spectrometry (ICP-MS) was used for the determination of monoiodotyrosine (MIT) and diiodotyrosine (DIT) in Edible Seaweed. A sample pre-treatment based on ultrasound assisted enzymatic hydrolysis was optimized for the extraction of these iodinated amino acids. Pancreatin was selected as the most adequate type of enzyme, and parameters affecting the extraction efficiency (pH, temperature, mass of enzyme and extraction time) were evaluated by univariate approaches. In addition, extractable inorganic iodine (iodide) was also quantified by anion exchange high performance liquid chromatography (AE-HPLC) coupled with ICP-MS. The proposed procedure offered limits of detection of 1.1 and 4.3ngg(-1) for MIT and DIT, respectively. Total iodine contents in Seaweed, as well as total iodine in enzymatic digests were measured by ICP-MS after microwave assisted alkaline digestion with tetramethylamonium hydroxide (TMAH) for total iodine assessment, and also by treating the pancreatin extracts (extractable total iodine assessment). The optimized procedure was successfully applied to five different types of Edible Seaweed. The highest total iodine content, and also the highest iodide levels, was found in the brown Seaweed Kombu (6646±45μgg(-1)). Regarding iodinated amino acids, Nori (a red Seaweed) was by far the one with the highest amount of both species (42±3 and 0.41±0.024μgg(-1) for MIT and DIT, respectively). In general, MIT concentrations were much higher than the amounts of DIT, which suggests that iodine from iodinated proteins in Seaweed is most likely bound in the form of MIT residues.
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speciation of the bio available iodine and bromine forms in Edible Seaweed by high performance liquid chromatography hyphenated with inductively coupled plasma mass spectrometry
Analytica Chimica Acta, 2012Co-Authors: Vanessa Romarishortas, Pilar Bermejobarrera, Jorge Moredapineiro, Antonio MoredapineiroAbstract:A bioavailability study based on an in vitro dialyzability approach has been applied to assess the bio-available fractions of iodine and bromine species from Edible Seaweed. Iodide, iodate, 3-iodo-tyrosine (MIT), 3,5-diiodo-tyrosine (DIT), bromide and bromate were separated by anion exchange chromatography under a gradient elution mode (175 mM ammonium nitrate plus 15% (v/v) methanol, pH 3.8, as a mobile phase, and flow rates within the 0.5-1.5 mL min(-1) range). Inductively coupled plasma-mass spectrometry (ICP-MS) was used as a selective detector for iodine ((127)I) and bromine ((79)Br). Low dialyzability ratios (within the 2.0-18% range) were found for iodine species; whereas, moderate dialyzability percentages (from 9.0 to 40%) were obtained for bromine species. Iodide and bromide were the major species found in the dialyzates from Seaweed, although MIT and bromate were also found in the dialyzates from most of the Seaweed samples analysed. However, DIT was only found in dialyzates from Wakame, Kombu, and NIES 09 (Sargasso) certified reference material; whereas, iodate was not found in any dialyzate. Iodine dialyzability was found to be dependent on the protein content (negative correlation), and on the carbohydrate and dietary fibre levels (positive correlation). However, bromine dialyzability was only dependent on the protein amount in Seaweed (negative correlation).
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development of anion exchange reversed phase high performance liquid chromatography inductively coupled plasma mass spectrometry methods for the speciation of bio available iodine and bromine from Edible Seaweed
Journal of Chromatography A, 2012Co-Authors: Vanessa Romarishortas, Pilar Bermejobarrera, Antonio MoredapineiroAbstract:Abstract Anion exchange high performance liquid chromatography hyphenated with inductively coupled plasma-mass spectrometry has been novelly applied to assess inorganic (iodide and iodate) and organic (3-iodotyrosine – MIT, and 3,5-diiodotyrosine – DIT) iodine species in a single chromatographic run. The optimized operating conditions (Dionex IonPac AS7, gradient elution with 175 mM ammonium nitrate plus 15% (v/v) methanol, pH 3.8, as a mobile phase and flow rates within the 0.5–1.5 mL min−1 range) have also been used to perform inorganic bromine speciation analysis (bromide and bromate). The developed method has been applied for determining the bio-available contents of iodine and bromine species in dialyzates from Edible Seaweed. Reverse phase high performance liquid chromatography (Zorbax Eclipse XDB-C8, gradient elution with 0.2% (m/m) acetic acid, and 0.2% (m/m) acetic acid in methanol, as mobile phases, and a constant flow rate of 0.75 mL min−1) also hyphenated with inductively coupled plasma-mass spectrometry was used to confirm the presence of organic iodine species (MIT and DIT) in the dialyzates. The verification of the presence of iodinated amino acids (MIT and DIT) in the extracts was also performed by reverse phase high performance liquid chromatography–electrospray ionization-mass spectrometry (LTQ Orbitrap). The developed methods have provided good repeatability (RSD values lower than 10% for both anion exchange and reverse phase separations) and analytical recoveries within the 90–105% range for all cases. The in vitro bio-availability method consisted of a simulated gastric and an intestinal digestion/dialysis (10 kDa molecular weight cut-off – MWCO) two-stage procedure. Iodide and MIT were the main bio-available species quantified, whereas bromide was the major bromine species found in the extracts.
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microwave assisted extraction of iodine and bromine from Edible Seaweed for inductively coupled plasma mass spectrometry determination
Talanta, 2009Co-Authors: Vanessa Romarishortas, Antonio Moredapineiro, Pilar BermejobarreraAbstract:The feasibility of microwave energy to assist the solubilisation of Edible Seaweed samples by tetramethylammonium hydroxide (TMAH) has been investigated to extract iodine and bromine. Inductively coupled plasma-mass spectrometry (ICP-MS) has been used as a multi-element detector. Variables affecting the microwave assisted extraction/solubilisation (temperature, TMAH volume, ramp time and hold time) were firstly screened by applying a fractional factorial design (2(5-1)+2), resolution V and 2 centre points. When extracting both halogens, results showed statistical significance (confidence interval of 95%) for TMAH volume and temperature, and also for the two order interaction between both variables. Therefore, these two variables were finally optimized by a 2(2)+star orthogonal central composite design with 5 centre points and 2 replicates, and optimum values of 200 degrees C and 10 mL for temperature and TMAH volume, respectively, were found. The extraction time (ramp and hold times) was found statistically non-significant, and values of 10 and 5 min were chosen for the ramp time and the hold time, respectively. This means a fast microwave heating cycle. Repeatability of the over-all procedure has been found to be 6% for both elements, while iodine and bromine concentrations of 24.6 and 19.9 ng g(-1), respectively, were established for the limit of detection. Accuracy of the method was assessed by analyzing the NIES-09 (Sargasso, Sargassum fulvellum) certified reference material (CRM) and the iodine and bromine concentrations found have been in good agreement with the indicative values for this CRM. Finally, the method was applied to several Edible dried and canned Seaweed samples.
Kwanghyun Baek - One of the best experts on this subject based on the ideXlab platform.
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Chemical characterization and antioxidant potential of volatile oil from an Edible Seaweed Porphyra tenera (Kjellman, 1897)
Chemistry Central Journal, 2017Co-Authors: Jayanta Kumar Patra, Seweon Lee, Jae Gyu Park, Yong-suk Kwon, Kwanghyun BaekAbstract:Background Porphyra tenera (Kjellman, 1897) is the most common eatable red Seaweed in Asia. In the present study, P. tenera volatile oil (PTVO) was extracted from dried P. tenera sheets that were used as food by the microwave hydrodistillation procedure, after which the characterization of its chemical constituents was done by gas chromatography and mass spectroscopy and its antioxidant potential was evaluated by a number of in vitro biochemical assays such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, nitric oxide (NO) scavenging, superoxide radical scavenging, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging, hydroxyl radical scavenging and reducing power assay and inhibition of lipid peroxidation. Results A total of 30 volatile compounds comprising about 99.4% of the total volume were identified, of which trans-beta-ionone (20.9%), hexadecanoic acid (9.2%) and 2,6-nonadienal (8.7%) were present in higher quantities. PTVO exhibited strong free radical scavenging activity by DPPH scavenging (44.62%), NO scavenging (28.45%) and superoxide scavenging (54.27%) at 500 µg/mL. Similarly, it displayed strong ABTS radical scavenging (IC_50 value of 177.83 µg/mL), hydroxyl radical scavenging (IC_50 value of 109.70 µg/mL), and moderate lipid peroxidation inhibition activity (IC_50 value of 231.80 µg/mL) and reducing power (IC_0.5 value of 126.58 µg/mL). PTVO exhibited strong antioxidant potential in a concentration dependent manner and the results were comparable with the BHT and α-tocopherol, taken as the reference standard compounds (positive controls). Conclusions Taken together, PTVO with potential bioactive chemical compounds and strong antioxidant activity could be utilized in the cosmetic industries for making antioxidant rich anti-aging and sun-screen lotion and in the food sector industries as food additives and preservatives.
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chemical characterization and antioxidant potential of volatile oil from an Edible Seaweed porphyra tenera kjellman 1897
Chemistry Central Journal, 2017Co-Authors: Jayanta Kumar Patra, Seweon Lee, Jae Gyu Park, Yong-suk Kwon, Kwanghyun BaekAbstract:Porphyra tenera (Kjellman, 1897) is the most common eatable red Seaweed in Asia. In the present study, P. tenera volatile oil (PTVO) was extracted from dried P. tenera sheets that were used as food by the microwave hydrodistillation procedure, after which the characterization of its chemical constituents was done by gas chromatography and mass spectroscopy and its antioxidant potential was evaluated by a number of in vitro biochemical assays such as 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, nitric oxide (NO) scavenging, superoxide radical scavenging, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging, hydroxyl radical scavenging and reducing power assay and inhibition of lipid peroxidation. A total of 30 volatile compounds comprising about 99.4% of the total volume were identified, of which trans-beta-ionone (20.9%), hexadecanoic acid (9.2%) and 2,6-nonadienal (8.7%) were present in higher quantities. PTVO exhibited strong free radical scavenging activity by DPPH scavenging (44.62%), NO scavenging (28.45%) and superoxide scavenging (54.27%) at 500 µg/mL. Similarly, it displayed strong ABTS radical scavenging (IC50 value of 177.83 µg/mL), hydroxyl radical scavenging (IC50 value of 109.70 µg/mL), and moderate lipid peroxidation inhibition activity (IC50 value of 231.80 µg/mL) and reducing power (IC0.5 value of 126.58 µg/mL). PTVO exhibited strong antioxidant potential in a concentration dependent manner and the results were comparable with the BHT and α-tocopherol, taken as the reference standard compounds (positive controls). Taken together, PTVO with potential bioactive chemical compounds and strong antioxidant activity could be utilized in the cosmetic industries for making antioxidant rich anti-aging and sun-screen lotion and in the food sector industries as food additives and preservatives.
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antioxidant and antibacterial properties of essential oil extracted from an Edible Seaweed undaria pinnatifida
Journal of Food Biochemistry, 2017Co-Authors: Jayanta Kumar Patra, Seweon Lee, Jae Gyu Park, Kwanghyun BaekAbstract:This study was conducted to extract essential oil from an Edible Seaweed, Undaria pinnatifida, and determine its chemical constituents, antibacterial and antioxidant potential. Gas chromatography and mass spectroscopy analysis of the U. pinnatifida essential oil (UPEO) revealed 26 volatile compounds comprising a total of 97.03%. Fatty acids such as tetradecanoic acid (31.32%) and hexadecanoic acid (22.39%) were present in the highest amount, followed by 3-hexen-1-ol (5.67%), erythritol (4.73%), 4-imidazolidinone (4.40%) and (9Z)-hexadec-9-enoic acid (4.37%). UPEO displayed potent antibacterial activity (9.5–11.0 mm inhibition zone), an MIC of 12.5–25.0 mg/mL and an MBC of 25.0 mg/mL. UPEO also exhibited strong hydroxyl radical scavenging (IC50 85.54 µg/mL), reducing power (IC0.5 178.82 µg/mL) and superoxide radical scavenging (IC50 260.89 µg/mL) potential. Taken together, these results indicate that UPEO could be an economical and safe resource for natural antioxidant and antibacterial compounds that can be utilized in food processing, cosmetics and pharmaceutical sectors. Practical Applications Seaweeds are a rich source of natural bioactive compounds with various medicinal properties. The essential oils extracted from an Edible Seaweed, U. pinnatifida, could serve as an alternative source of natural antioxidant compounds with the potential for use as food additives and in food preservation and cosmetics. Use of this compound could minimize the use of synthetic antioxidant compounds that trigger health hazards. Furthermore, the bioactive compounds present in the UPEO could serve as a source of natural antibiotics against both Gram positive and Gram negative pathogenic bacteria.
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antibacterial activity and action mechanism of the essential oil from enteromorpha linza l against foodborne pathogenic bacteria
Molecules, 2016Co-Authors: Jayanta Kumar Patra, Kwanghyun BaekAbstract:Foodborne illness and disease caused by foodborne pathogenic bacteria is continuing to increase day by day and it has become an important topic of concern among various food industries. Many types of synthetic antibacterial agents have been used in food processing and food preservation; however, they are not safe and have resulted in various health-related issues. Therefore, in the present study, essential oil from an Edible Seaweed, Enteromorpha linza (AEO), was evaluated for its antibacterial activity against foodborne pathogens, along with the mechanism of its antibacterial action. AEO at 25 mg/disc was highly active against Bacillus cereus (12.3-12.7 mm inhibition zone) and Staphylococcus aureus (12.7-13.3 mm inhibition zone). The minimum inhibitory concentration and minimum bactericidal concentration values of AEO ranged from 12.5-25 mg/mL. Further investigation of the mechanism of action of AEO revealed its strong impairing effect on the viability of bacterial cells and membrane permeability, as indicated by a significant increase in leakage of 260 nm absorbing materials and K⁺ ions from the cell membrane and loss of high salt tolerance. Taken together, these data suggest that AEO has the potential for use as an effective antibacterial agent that functions by impairing cell membrane permeability via morphological alternations, resulting in cellular lysis and cell death.
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chemical composition and antioxidant and antibacterial activities of an essential oil extracted from an Edible Seaweed laminaria japonica l
Molecules, 2015Co-Authors: Jayanta Kumar Patra, Gitishree Das, Kwanghyun BaekAbstract:Laminaria japonica L. is among the most commonly consumed Seaweeds in northeast Asia. In the present study, L. japonica essential oil (LJEO) was extracted by microwave-hydrodistillation and analyzed by gas chromatography and mass spectroscopy. LJEO contained 21 volatile compounds, comprising 99.76% of the total volume of the essential oil, primarily tetradeconoic acid (51.75%), hexadecanoic acid (16.57%), (9Z,12Z)-9,12-Octadecadienoic acid (12.09%), and (9Z)-hexadec-9-enoic acid (9.25%). Evaluation of the antibacterial potential against three foodborne pathogens, Bacillus cereus ATCC 10876, Escherichia coli O157:H7 ATCC 43890, and Staphylococcus aureus ATCC 49444, revealed that LJEO at a concentration of 25 mg/paper disc exerted high antibacterial activity against S. aureus (11.5 ± 0.58 mm inhibition zone) and B. cereus (10.5 ± 0.57 mm inhibition zone), but no inhibition of E. coli O157:H7. LJEO also displayed DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical scavenging activity (80.45%), superoxide anion scavenging activity (54.03%), and ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical and hydroxyl radical scavenging at 500 µg/mL. Finally, LJEO showed high inhibition of lipid peroxidation with strong reducing power. In conclusion, LJEO from Edible Seaweed is an inexpensive but favorable resource with strong antibacterial capacity as well as free radical scavenging and antioxidant activity; therefore, it has the potential for use in the food, cosmetics, and pharmaceutical industries.
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phlorotannins from ecklonia cava phaeophyceae biological activities and potential health benefits
Biofactors, 2010Co-Authors: Isuru Wijesekara, Na Young YoonAbstract:The importance of bioactive derivatives as functional ingredients has been well recognized due to their valuable health beneficial effects. Therefore, isolation and characterization of novel functional ingredients with biological activities from Seaweeds have gained much attention. Ecklonia cava Kjellman is an Edible Seaweed, which has been recognized as a rich source of bioactive derivatives mainly, phlorotannins. These phlorotannins exhibit various beneficial biological activities such as antioxidant, anticancer, antidiabetic, anti-human immunodeficiency virus, antihypertensive, matrix metalloproteinase enzyme inhibition, hyaluronidase enzyme inhibition, radioprotective, and antiallergic activities. This review focuses on biological activities of phlorotannins with potential health beneficial applications in functional foods, pharmaceuticals, and cosmeceuticals. © 2010 International Union of Biochemistry and Molecular Biology, Inc.
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Phlorotannins from Ecklonia cava (Phaeophyceae): biological activities and potential health benefits.
BioFactors, 2010Co-Authors: Isuru Wijesekara, Na Young YoonAbstract:The importance of bioactive derivatives as functional ingredients has been well recognized due to their valuable health beneficial effects. Therefore, isolation and characterization of novel functional ingredients with biological activities from Seaweeds have gained much attention. Ecklonia cava Kjellman is an Edible Seaweed, which has been recognized as a rich source of bioactive derivatives mainly, phlorotannins. These phlorotannins exhibit various beneficial biological activities such as antioxidant, anticancer, antidiabetic, anti-human immunodeficiency virus, antihypertensive, matrix metalloproteinase enzyme inhibition, hyaluronidase enzyme inhibition, radioprotective, and antiallergic activities. This review focuses on biological activities of phlorotannins with potential health beneficial applications in functional foods, pharmaceuticals, and cosmeceuticals.
