The Experts below are selected from a list of 2076 Experts worldwide ranked by ideXlab platform
Chun Yang - One of the best experts on this subject based on the ideXlab platform.
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cells sensing mechanical cues stiffness influences the lifetime of cell extracellular matrix interactions by affecting the loading rate
ACS Nano, 2016Co-Authors: Li Jiang, Zhenglong Sun, Xiaofei Chen, Dong Han, Chun YangAbstract:The question of how cells sense substrate mechanical cues has gained increasing attention among biologists. By introducing contour-based data analysis to single-cell force spectroscopy, we identified a loading-rate threshold for the integrin α2β1–DGEA bond beyond which a dramatic increase in bond lifetime was observed. On the basis of mechanical cues (elasticity or topography), the Effective Spring Constant of substrates k is mapped to the loading rate r under actomyosin pulling speed v, which, in turn, affects the lifetime of the integrin–ligand bond. Additionally, downregulating v with a low-dose blebbistatin treatment promotes the neuronal lineage specification of mesenchymal stem cells on osteogenic stiff substrates. Thus, sensing of the loading rate is central to how cells sense mechanical cues that affect cell–extracellular matrix interactions and stem cell differentiation.
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Cells Sensing Mechanical Cues: Stiffness Influences the Lifetime of Cell–Extracellular Matrix Interactions by Affecting the Loading Rate
2016Co-Authors: Li Jiang, Zhenglong Sun, Xiaofei Chen, Dong Han, Chun YangAbstract:The question of how cells sense substrate mechanical cues has gained increasing attention among biologists. By introducing contour-based data analysis to single-cell force spectroscopy, we identified a loading-rate threshold for the integrin α2β1–DGEA bond beyond which a dramatic increase in bond lifetime was observed. On the basis of mechanical cues (elasticity or topography), the Effective Spring Constant of substrates k is mapped to the loading rate r under actomyosin pulling speed v, which, in turn, affects the lifetime of the integrin–ligand bond. Additionally, downregulating v with a low-dose blebbistatin treatment promotes the neuronal lineage specification of mesenchymal stem cells on osteogenic stiff substrates. Thus, sensing of the loading rate is central to how cells sense mechanical cues that affect cell–extracellular matrix interactions and stem cell differentiation
Zhenglong Sun - One of the best experts on this subject based on the ideXlab platform.
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cells sensing mechanical cues stiffness influences the lifetime of cell extracellular matrix interactions by affecting the loading rate
ACS Nano, 2016Co-Authors: Li Jiang, Zhenglong Sun, Xiaofei Chen, Dong Han, Chun YangAbstract:The question of how cells sense substrate mechanical cues has gained increasing attention among biologists. By introducing contour-based data analysis to single-cell force spectroscopy, we identified a loading-rate threshold for the integrin α2β1–DGEA bond beyond which a dramatic increase in bond lifetime was observed. On the basis of mechanical cues (elasticity or topography), the Effective Spring Constant of substrates k is mapped to the loading rate r under actomyosin pulling speed v, which, in turn, affects the lifetime of the integrin–ligand bond. Additionally, downregulating v with a low-dose blebbistatin treatment promotes the neuronal lineage specification of mesenchymal stem cells on osteogenic stiff substrates. Thus, sensing of the loading rate is central to how cells sense mechanical cues that affect cell–extracellular matrix interactions and stem cell differentiation.
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Cells Sensing Mechanical Cues: Stiffness Influences the Lifetime of Cell–Extracellular Matrix Interactions by Affecting the Loading Rate
2016Co-Authors: Li Jiang, Zhenglong Sun, Xiaofei Chen, Dong Han, Chun YangAbstract:The question of how cells sense substrate mechanical cues has gained increasing attention among biologists. By introducing contour-based data analysis to single-cell force spectroscopy, we identified a loading-rate threshold for the integrin α2β1–DGEA bond beyond which a dramatic increase in bond lifetime was observed. On the basis of mechanical cues (elasticity or topography), the Effective Spring Constant of substrates k is mapped to the loading rate r under actomyosin pulling speed v, which, in turn, affects the lifetime of the integrin–ligand bond. Additionally, downregulating v with a low-dose blebbistatin treatment promotes the neuronal lineage specification of mesenchymal stem cells on osteogenic stiff substrates. Thus, sensing of the loading rate is central to how cells sense mechanical cues that affect cell–extracellular matrix interactions and stem cell differentiation
John E Sader - One of the best experts on this subject based on the ideXlab platform.
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effect of surface stress on the stiffness of thin elastic plates and beams
Physical Review B, 2012Co-Authors: Michael J Lachut, John E SaderAbstract:Nanomechanical doubly-clamped beams and cantilever plates are often used to sense a host of environmental effects, including biomolecular interations, mass measurements, and responses to chemical stimuli. Understanding the effects of surface stress on the stiffness of such nanoscale devices is essential for rigorous analysis of experimental data. Recently, we explored the effects of surface stress on cantilever plates and presented a theoretical framework valid for thin plate structures. Here, we generalize this framework and apply it to cantilever plates and doubly-clamped beams, exploring in detail the relative physical mechanisms causing a stiffness change in each case. Specifically, Poisson’s ratio is found to exert a dramatically different effect in cantilevers than in doubly-clamped beams, and here we explain why. The relative change in Effective Spring Constant is also examined, and its connection to the relative frequency shift is discussed. Interestingly, this differs from what is naively expected from elementary mechanics. Finally, a discussion of the practical implications of our theoretical findings is presented, which includes an assessment of available experimental results and potential future measurements on nanoscale devices.
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effect of surface stress on the stiffness of thin elastic plates and beams
Physical Review B, 2012Co-Authors: Michael J Lachut, John E SaderAbstract:Nanomechanical doubly-clamped beams and cantilever plates are often used to sense a host of environmental effects, including biomolecular interations, mass measurements, and responses to chemical stimuli. Understanding the effects of surface stress on the stiffness of such nanoscale devices is essential for rigorous analysis of experimental data. Recently, we explored the effects of surface stress on cantilever plates and presented a theoretical framework valid for thin plate structures. Here, we generalize this framework and apply it to cantilever plates and doubly-clamped beams, exploring in detail the relative physical mechanisms causing a stiffness change in each case. Specifically, Poisson's ratio is found to exert a dramatically different effect in cantilevers than in doubly-clamped beams, and here we explain why. The relative change in Effective Spring Constant is also examined, and its connection to the relative frequency shift is discussed. Interestingly, this differs from what is naively expected from elementary mechanics. Finally, a discussion of the practical implications of our theoretical findings is presented, which includes an assessment of available experimental results and potential future measurements on nanoscale devices. © 2012 American Physical Society.
Li Jiang - One of the best experts on this subject based on the ideXlab platform.
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cells sensing mechanical cues stiffness influences the lifetime of cell extracellular matrix interactions by affecting the loading rate
ACS Nano, 2016Co-Authors: Li Jiang, Zhenglong Sun, Xiaofei Chen, Dong Han, Chun YangAbstract:The question of how cells sense substrate mechanical cues has gained increasing attention among biologists. By introducing contour-based data analysis to single-cell force spectroscopy, we identified a loading-rate threshold for the integrin α2β1–DGEA bond beyond which a dramatic increase in bond lifetime was observed. On the basis of mechanical cues (elasticity or topography), the Effective Spring Constant of substrates k is mapped to the loading rate r under actomyosin pulling speed v, which, in turn, affects the lifetime of the integrin–ligand bond. Additionally, downregulating v with a low-dose blebbistatin treatment promotes the neuronal lineage specification of mesenchymal stem cells on osteogenic stiff substrates. Thus, sensing of the loading rate is central to how cells sense mechanical cues that affect cell–extracellular matrix interactions and stem cell differentiation.
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Cells Sensing Mechanical Cues: Stiffness Influences the Lifetime of Cell–Extracellular Matrix Interactions by Affecting the Loading Rate
2016Co-Authors: Li Jiang, Zhenglong Sun, Xiaofei Chen, Dong Han, Chun YangAbstract:The question of how cells sense substrate mechanical cues has gained increasing attention among biologists. By introducing contour-based data analysis to single-cell force spectroscopy, we identified a loading-rate threshold for the integrin α2β1–DGEA bond beyond which a dramatic increase in bond lifetime was observed. On the basis of mechanical cues (elasticity or topography), the Effective Spring Constant of substrates k is mapped to the loading rate r under actomyosin pulling speed v, which, in turn, affects the lifetime of the integrin–ligand bond. Additionally, downregulating v with a low-dose blebbistatin treatment promotes the neuronal lineage specification of mesenchymal stem cells on osteogenic stiff substrates. Thus, sensing of the loading rate is central to how cells sense mechanical cues that affect cell–extracellular matrix interactions and stem cell differentiation
St́phanie P. Lacour - One of the best experts on this subject based on the ideXlab platform.
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engineering reversible elasticity in ductile and brittle thin films supported by a plastic foil
Extreme Mechanics Letters, 2017Co-Authors: Nicolas Vachicouras, Christina M Tringides, Philippe B Campiche, St́phanie P. LacourAbstract:Abstract Reversible deformation is a unique property of elastic materials. Here, we design and fabricate highly stretchable multilayered films by patterning Y-shaped motifs through films of non-elastic materials, e.g. plastics, metals, ceramics. By adjusting the geometry and density of the motif, as well as the thickness of the film(s), the Effective Spring Constant of the engineered film(s) can be tuned. Three-dimensional bending of the patterned film(s) enables macroscopic stretchability and minimizes local film strain fields. The engineered films demonstrate no preferential direction of stretching and the proposed design is versatile. Furthermore our approach is compatible with thin-film processing. We demonstrate the Y-shaped motifs allow for the design of stretchable plastic foils coated with metallic and metal oxide conductors. We anticipate the patterned motifs can be scaled down to offer a wider range of elastic electronic materials to use in stretchable electronics and to create soft bioelectronics.
