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Julio Alarcon - One of the best experts on this subject based on the ideXlab platform.
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phytochemical profile and the antioxidant activity of chilean wild black berry fruits aristotelia chilensis mol stuntz Elaeocarpaceae
Food Chemistry, 2010Co-Authors: Carlos L Cespedes, Mohammed Elhafidi, Julio Alarcon, Maribel Valdezmorales, Jose G Avila, Octavio ParedeslopezAbstract:Abstract From ethanolic, water extracts and their fractions of mature fruits of wild black-berry Aristotelia chilensis (Mol) Stuntz (Elaeocarpaceae), different phenolic compounds were identified by chromatographic (HPLC) and unequivocally assignments by spectroscopic (UV, NMR) data analysis. Anthocyanidins, flavonoids and phenolic acids fractions were obtained using flash and open column chromatography. The main compounds gentisic acid, ferulic acid, gallic acid, p-coumaric acid, sinapic acid, 4-hydroxybenzoic acid, delphinidin, cyanidin, vanillic acid, delphinidin gallate, gallocatechin gallate, quercetin, rutin, myricetin, catechin and epi-catechin as mixture 1:1, and several glycosides of anthocyanidins (delphinidin-3-sambubioside-5-glucoside, delphinidin-3,5-diglucoside, cyanidin-3-sambubioside-5-glucoside, cyanidin-3,5-diglucoside, delphinidin-3-sambubioside, delphinidin-3-glucoside, cyanidin-3-sambubioside, and cyanidin-3-glucoside), and proanthocyanidin B were detected. In addition to phytochemical analysis the antioxidant activities of extracts, partitions and fractions were strongly correlated with the highest polyphenol contents. The most active samples were the ethanolic and acetone extracts in all bioassays used and all samples were compared for activity against butylated hydroxy toluene (BHT), quercetin and tocopherol used as pattern samples. The juice (E), EtOH extract (A) and acetone partition (B) were found to have IC50 values of 4.7, 1.7 and 7.4 ppm, respectively against DPPH and 5.9, 2.1 and 3.9 ppm, respectively against TBARS formation. Additionally, the fraction F-4 showed a strong activity with IC50 of 4.9 and 6.5 ppm, against DPPH and TBARS respectively. Consistent with this finding, EtOH extract had the greatest ORAC and FRAP values as percentage of activity. On the other hand the IC50 values for the inhibitory activity against O 2 - of extract B, F-3 and F-4 were 9.7, 13.2 and 10.7 ppm, respectively and against OH − were 29.1, 7.0 and 6.3 ppm, respectively. The EtOH extract protects against stress oxidative reducing the concentration of the MDA a lipid peroxidation index. These results shows that this fruit could be useful as antioxidant and nutraceutical sources.
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antioxidant and cardioprotective activities of phenolic extracts from fruits of chilean blackberry aristotelia chilensis Elaeocarpaceae maqui
Food Chemistry, 2008Co-Authors: Carlos L Cespedes, Mohammed Elhafidi, Natalia Pavon, Julio AlarconAbstract:Abstract The methanol extract from mature fruits of Aristotelia chilensis (Mol) Stuntz (Elaeocarpaceae) showed antioxidant activities and cardioprotective effects on acute ischemia/reperfusion performed in rat heart in vivo. This extract protected animals from heart damage by the incidence of reperfusion dysrythmias, and the no-recovery of sinus rhythm. On the other hand, the MeOH extract of the fruit was able to prevent these harmful events in the animal’s heart by diminishing lipid oxidation and reducing the concentration of thiobarbituric acid reactive substances (TBARS), a lipid peroxidation index. In addition, MeOH extract of A. chilensis was evaluated for DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging, crocin radical scavenging, oxygen radical absorption capacity (ORAC), ferric reducing antioxidant power (FRAP), an estimation of lipid peroxidation in liposomes through the inhibition of formation of TBARS. MeOH extract was found to have IC50 of 1.62 ppm against DPPH and 2.51 ppm against TBARS, compared with the juice, whose IC50 was 12.1 ppm and 9.58 ppm against DPPH and TBARS formation, respectively. Antioxidant activities of MeOH extract were strongly correlated with total polyphenol content. Consistent with this finding, MeOH had the greatest ORAC and FRAP values as percentage of activity. These results show that these fruits could be useful as antioxidant, cardioprotective and nutraceutical sources.
Carlos L Cespedes - One of the best experts on this subject based on the ideXlab platform.
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phytochemical profile and the antioxidant activity of chilean wild black berry fruits aristotelia chilensis mol stuntz Elaeocarpaceae
Food Chemistry, 2010Co-Authors: Carlos L Cespedes, Mohammed Elhafidi, Julio Alarcon, Maribel Valdezmorales, Jose G Avila, Octavio ParedeslopezAbstract:Abstract From ethanolic, water extracts and their fractions of mature fruits of wild black-berry Aristotelia chilensis (Mol) Stuntz (Elaeocarpaceae), different phenolic compounds were identified by chromatographic (HPLC) and unequivocally assignments by spectroscopic (UV, NMR) data analysis. Anthocyanidins, flavonoids and phenolic acids fractions were obtained using flash and open column chromatography. The main compounds gentisic acid, ferulic acid, gallic acid, p-coumaric acid, sinapic acid, 4-hydroxybenzoic acid, delphinidin, cyanidin, vanillic acid, delphinidin gallate, gallocatechin gallate, quercetin, rutin, myricetin, catechin and epi-catechin as mixture 1:1, and several glycosides of anthocyanidins (delphinidin-3-sambubioside-5-glucoside, delphinidin-3,5-diglucoside, cyanidin-3-sambubioside-5-glucoside, cyanidin-3,5-diglucoside, delphinidin-3-sambubioside, delphinidin-3-glucoside, cyanidin-3-sambubioside, and cyanidin-3-glucoside), and proanthocyanidin B were detected. In addition to phytochemical analysis the antioxidant activities of extracts, partitions and fractions were strongly correlated with the highest polyphenol contents. The most active samples were the ethanolic and acetone extracts in all bioassays used and all samples were compared for activity against butylated hydroxy toluene (BHT), quercetin and tocopherol used as pattern samples. The juice (E), EtOH extract (A) and acetone partition (B) were found to have IC50 values of 4.7, 1.7 and 7.4 ppm, respectively against DPPH and 5.9, 2.1 and 3.9 ppm, respectively against TBARS formation. Additionally, the fraction F-4 showed a strong activity with IC50 of 4.9 and 6.5 ppm, against DPPH and TBARS respectively. Consistent with this finding, EtOH extract had the greatest ORAC and FRAP values as percentage of activity. On the other hand the IC50 values for the inhibitory activity against O 2 - of extract B, F-3 and F-4 were 9.7, 13.2 and 10.7 ppm, respectively and against OH − were 29.1, 7.0 and 6.3 ppm, respectively. The EtOH extract protects against stress oxidative reducing the concentration of the MDA a lipid peroxidation index. These results shows that this fruit could be useful as antioxidant and nutraceutical sources.
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antioxidant and cardioprotective activities of phenolic extracts from fruits of chilean blackberry aristotelia chilensis Elaeocarpaceae maqui
Food Chemistry, 2008Co-Authors: Carlos L Cespedes, Mohammed Elhafidi, Natalia Pavon, Julio AlarconAbstract:Abstract The methanol extract from mature fruits of Aristotelia chilensis (Mol) Stuntz (Elaeocarpaceae) showed antioxidant activities and cardioprotective effects on acute ischemia/reperfusion performed in rat heart in vivo. This extract protected animals from heart damage by the incidence of reperfusion dysrythmias, and the no-recovery of sinus rhythm. On the other hand, the MeOH extract of the fruit was able to prevent these harmful events in the animal’s heart by diminishing lipid oxidation and reducing the concentration of thiobarbituric acid reactive substances (TBARS), a lipid peroxidation index. In addition, MeOH extract of A. chilensis was evaluated for DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging, crocin radical scavenging, oxygen radical absorption capacity (ORAC), ferric reducing antioxidant power (FRAP), an estimation of lipid peroxidation in liposomes through the inhibition of formation of TBARS. MeOH extract was found to have IC50 of 1.62 ppm against DPPH and 2.51 ppm against TBARS, compared with the juice, whose IC50 was 12.1 ppm and 9.58 ppm against DPPH and TBARS formation, respectively. Antioxidant activities of MeOH extract were strongly correlated with total polyphenol content. Consistent with this finding, MeOH had the greatest ORAC and FRAP values as percentage of activity. These results show that these fruits could be useful as antioxidant, cardioprotective and nutraceutical sources.
Cesar Oswaldo - One of the best experts on this subject based on the ideXlab platform.
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Floristic composition of a riverine forest in the Brazilian Atlantic rain forest, Ubatuba, SP
Sociedade Botânica de São Paulo, 2015Co-Authors: Sanchez Maryland, Pedroni Fernando, LeitÃo-filho, HermÓgenes De Freitas, Cesar OswaldoAbstract:A floristic survey was carried out in a forest tract alongside the Fazenda river, in the Picinguaba Division of the Serra do Mar State Park, northern coast of the State of São Paulo, municipality of Ubatuba (44°48' W and 23°22' S). A total of 40 quadrats (10 m x 10 m) was located along the river margins. All trees with a minimum of 20 cm perimeter at breast height (DBH = 6.36 cm) were recorded. The 673 sampled trees were distributed in 120 species, 83 genera and 37 families. The greatest species richness was found for Myrtaceae (28), Fabaceae (11), Rubiaceae and Lauraceae (8). The most numerous populations were Euterpe edulis Mart. (Arecaceae), Chrysophyllum flexuosum Mart. (Sapotaceae), Coussarea nodosa Müll. Arg. (Rubiaceae) and Sloanea guianensis Benth. (Elaeocarpaceae). The Shannon diversity index was H' = 4.07 nats/individual, showing the great diversity of the area studied. The data emphasize the high complexity of the vegetation studied and are used for comparisons with similar forests.O presente trabalho trata do estudo florístico da vegetação arbórea que ocorre ao longo de um trecho das margens do rio da Fazenda, localizado no Parque Estadual da Serra do Mar, Núcleo de Picinguaba, no litoral norte do estado de São Paulo, município de Ubatuba (44°48' W e 23°22' S). Para a realização do levantamento, foram alocadas 40 parcelas de 10 m x 10 m, ao longo de um trecho do rio, sendo amostrados todos indivíduos com perímetro à altura do peito maior ou igual a 20 cm (DAP = 6,36 cm). Os 673 indivíduos amostrados distribuíram-se entre 120 espécies, 83 gêneros e 37 famílias. Myrtaceae foi a família de maior riqueza, com 28 espécies, seguida por Fabaceae (11), Rubiaceae e Lauraceae (8 cada uma). Euterpe edulis Mart. (Arecaceae), Chrysophyllum flexuosum Mart. (Sapotaceae), Coussarea nodosa Müll. Arg. (Rubiaceae) e Sloanea guianensis Benth. (Elaeocarpaceae) foram as populações com maior número de indivíduos. O índice de diversidade de Shannon encontrado foi H' = 4,07 nats/indivíduo, mostrando a grande diversidade da vegetação na área de estudo. As informações obtidas neste trabalho ressaltam a alta complexidade da Floresta Pluvial Tropical da Encosta Atlântica ao longo de sua extensão e fornecem dados para estudos comparativos com outros trechos de Mata Atlântica ou mesmo com outras formações florestais.3142Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
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Floristic composition of a riverine forest in the Brazilian Atlantic rain forest, Ubatuba, SP
Sociedade Botânica de São Paulo, 2015Co-Authors: Sanchez Maryland, Pedroni Fernando, LeitÃo-filho, HermÓgenes De Freitas, Cesar OswaldoAbstract:A floristic survey was carried out in a forest tract alongside the Fazenda river, in the Picinguaba Division of the Serra do Mar State Park, northern coast of the State of São Paulo, municipality of Ubatuba (44°48' W and 23°22' S). A total of 40 quadrats (10 m x 10 m) was located along the river margins. All trees with a minimum of 20 cm perimeter at breast height (DBH = 6.36 cm) were recorded. The 673 sampled trees were distributed in 120 species, 83 genera and 37 families. The greatest species richness was found for Myrtaceae (28), Fabaceae (11), Rubiaceae and Lauraceae (8). The most numerous populations were Euterpe edulis Mart. (Arecaceae), Chrysophyllum flexuosum Mart. (Sapotaceae), Coussarea nodosa Müll. Arg. (Rubiaceae) and Sloanea guianensis Benth. (Elaeocarpaceae). The Shannon diversity index was H' = 4.07 nats/individual, showing the great diversity of the area studied. The data emphasize the high complexity of the vegetation studied and are used for comparisons with similar forests.O presente trabalho trata do estudo florístico da vegetação arbórea que ocorre ao longo de um trecho das margens do rio da Fazenda, localizado no Parque Estadual da Serra do Mar, Núcleo de Picinguaba, no litoral norte do estado de São Paulo, município de Ubatuba (44°48' W e 23°22' S). Para a realização do levantamento, foram alocadas 40 parcelas de 10 m x 10 m, ao longo de um trecho do rio, sendo amostrados todos indivíduos com perímetro à altura do peito maior ou igual a 20 cm (DAP = 6,36 cm). Os 673 indivíduos amostrados distribuíram-se entre 120 espécies, 83 gêneros e 37 famílias. Myrtaceae foi a família de maior riqueza, com 28 espécies, seguida por Fabaceae (11), Rubiaceae e Lauraceae (8 cada uma). Euterpe edulis Mart. (Arecaceae), Chrysophyllum flexuosum Mart. (Sapotaceae), Coussarea nodosa Müll. Arg. (Rubiaceae) e Sloanea guianensis Benth. (Elaeocarpaceae) foram as populações com maior número de indivíduos. O índice de diversidade de Shannon encontrado foi H' = 4,07 nats/indivíduo, mostrando a grande diversidade da vegetação na área de estudo. As informações obtidas neste trabalho ressaltam a alta complexidade da Floresta Pluvial Tropical da Encosta Atlântica ao longo de sua extensão e fornecem dados para estudos comparativos com outros trechos de Mata Atlântica ou mesmo com outras formações florestais
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Composição florística de um trecho de floresta ripária na Mata Atlântica em Picinguaba, Ubatuba, SP
Sociedade Botânica de São Paulo, 1999Co-Authors: Sanchez Maryland, Pedroni Fernando, LeitÃo-filho, HermÓgenes De Freitas, Cesar OswaldoAbstract:O presente trabalho trata do estudo florístico da vegetação arbórea que ocorre ao longo de um trecho das margens do rio da Fazenda, localizado no Parque Estadual da Serra do Mar, Núcleo de Picinguaba, no litoral norte do estado de São Paulo, município de Ubatuba (44°48' W e 23°22' S). Para a realização do levantamento, foram alocadas 40 parcelas de 10 m x 10 m, ao longo de um trecho do rio, sendo amostrados todos indivíduos com perímetro à altura do peito maior ou igual a 20 cm (DAP = 6,36 cm). Os 673 indivíduos amostrados distribuíram-se entre 120 espécies, 83 gêneros e 37 famílias. Myrtaceae foi a família de maior riqueza, com 28 espécies, seguida por Fabaceae (11), Rubiaceae e Lauraceae (8 cada uma). Euterpe edulis Mart. (Arecaceae), Chrysophyllum flexuosum Mart. (Sapotaceae), Coussarea nodosa Müll. Arg. (Rubiaceae) e Sloanea guianensis Benth. (Elaeocarpaceae) foram as populações com maior número de indivíduos. O índice de diversidade de Shannon encontrado foi H' = 4,07 nats/indivíduo, mostrando a grande diversidade da vegetação na área de estudo. As informações obtidas neste trabalho ressaltam a alta complexidade da Floresta Pluvial Tropical da Encosta Atlântica ao longo de sua extensão e fornecem dados para estudos comparativos com outros trechos de Mata Atlântica ou mesmo com outras formações florestais.A floristic survey was carried out in a forest tract alongside the Fazenda river, in the Picinguaba Division of the Serra do Mar State Park, northern coast of the State of São Paulo, municipality of Ubatuba (44°48' W and 23°22' S). A total of 40 quadrats (10 m x 10 m) was located along the river margins. All trees with a minimum of 20 cm perimeter at breast height (DBH = 6.36 cm) were recorded. The 673 sampled trees were distributed in 120 species, 83 genera and 37 families. The greatest species richness was found for Myrtaceae (28), Fabaceae (11), Rubiaceae and Lauraceae (8). The most numerous populations were Euterpe edulis Mart. (Arecaceae), Chrysophyllum flexuosum Mart. (Sapotaceae), Coussarea nodosa Müll. Arg. (Rubiaceae) and Sloanea guianensis Benth. (Elaeocarpaceae). The Shannon diversity index was H' = 4.07 nats/individual, showing the great diversity of the area studied. The data emphasize the high complexity of the vegetation studied and are used for comparisons with similar forests.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES
Mohammed Elhafidi - One of the best experts on this subject based on the ideXlab platform.
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phytochemical profile and the antioxidant activity of chilean wild black berry fruits aristotelia chilensis mol stuntz Elaeocarpaceae
Food Chemistry, 2010Co-Authors: Carlos L Cespedes, Mohammed Elhafidi, Julio Alarcon, Maribel Valdezmorales, Jose G Avila, Octavio ParedeslopezAbstract:Abstract From ethanolic, water extracts and their fractions of mature fruits of wild black-berry Aristotelia chilensis (Mol) Stuntz (Elaeocarpaceae), different phenolic compounds were identified by chromatographic (HPLC) and unequivocally assignments by spectroscopic (UV, NMR) data analysis. Anthocyanidins, flavonoids and phenolic acids fractions were obtained using flash and open column chromatography. The main compounds gentisic acid, ferulic acid, gallic acid, p-coumaric acid, sinapic acid, 4-hydroxybenzoic acid, delphinidin, cyanidin, vanillic acid, delphinidin gallate, gallocatechin gallate, quercetin, rutin, myricetin, catechin and epi-catechin as mixture 1:1, and several glycosides of anthocyanidins (delphinidin-3-sambubioside-5-glucoside, delphinidin-3,5-diglucoside, cyanidin-3-sambubioside-5-glucoside, cyanidin-3,5-diglucoside, delphinidin-3-sambubioside, delphinidin-3-glucoside, cyanidin-3-sambubioside, and cyanidin-3-glucoside), and proanthocyanidin B were detected. In addition to phytochemical analysis the antioxidant activities of extracts, partitions and fractions were strongly correlated with the highest polyphenol contents. The most active samples were the ethanolic and acetone extracts in all bioassays used and all samples were compared for activity against butylated hydroxy toluene (BHT), quercetin and tocopherol used as pattern samples. The juice (E), EtOH extract (A) and acetone partition (B) were found to have IC50 values of 4.7, 1.7 and 7.4 ppm, respectively against DPPH and 5.9, 2.1 and 3.9 ppm, respectively against TBARS formation. Additionally, the fraction F-4 showed a strong activity with IC50 of 4.9 and 6.5 ppm, against DPPH and TBARS respectively. Consistent with this finding, EtOH extract had the greatest ORAC and FRAP values as percentage of activity. On the other hand the IC50 values for the inhibitory activity against O 2 - of extract B, F-3 and F-4 were 9.7, 13.2 and 10.7 ppm, respectively and against OH − were 29.1, 7.0 and 6.3 ppm, respectively. The EtOH extract protects against stress oxidative reducing the concentration of the MDA a lipid peroxidation index. These results shows that this fruit could be useful as antioxidant and nutraceutical sources.
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antioxidant and cardioprotective activities of phenolic extracts from fruits of chilean blackberry aristotelia chilensis Elaeocarpaceae maqui
Food Chemistry, 2008Co-Authors: Carlos L Cespedes, Mohammed Elhafidi, Natalia Pavon, Julio AlarconAbstract:Abstract The methanol extract from mature fruits of Aristotelia chilensis (Mol) Stuntz (Elaeocarpaceae) showed antioxidant activities and cardioprotective effects on acute ischemia/reperfusion performed in rat heart in vivo. This extract protected animals from heart damage by the incidence of reperfusion dysrythmias, and the no-recovery of sinus rhythm. On the other hand, the MeOH extract of the fruit was able to prevent these harmful events in the animal’s heart by diminishing lipid oxidation and reducing the concentration of thiobarbituric acid reactive substances (TBARS), a lipid peroxidation index. In addition, MeOH extract of A. chilensis was evaluated for DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging, crocin radical scavenging, oxygen radical absorption capacity (ORAC), ferric reducing antioxidant power (FRAP), an estimation of lipid peroxidation in liposomes through the inhibition of formation of TBARS. MeOH extract was found to have IC50 of 1.62 ppm against DPPH and 2.51 ppm against TBARS, compared with the juice, whose IC50 was 12.1 ppm and 9.58 ppm against DPPH and TBARS formation, respectively. Antioxidant activities of MeOH extract were strongly correlated with total polyphenol content. Consistent with this finding, MeOH had the greatest ORAC and FRAP values as percentage of activity. These results show that these fruits could be useful as antioxidant, cardioprotective and nutraceutical sources.
Octavio Paredeslopez - One of the best experts on this subject based on the ideXlab platform.
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phytochemical profile and the antioxidant activity of chilean wild black berry fruits aristotelia chilensis mol stuntz Elaeocarpaceae
Food Chemistry, 2010Co-Authors: Carlos L Cespedes, Mohammed Elhafidi, Julio Alarcon, Maribel Valdezmorales, Jose G Avila, Octavio ParedeslopezAbstract:Abstract From ethanolic, water extracts and their fractions of mature fruits of wild black-berry Aristotelia chilensis (Mol) Stuntz (Elaeocarpaceae), different phenolic compounds were identified by chromatographic (HPLC) and unequivocally assignments by spectroscopic (UV, NMR) data analysis. Anthocyanidins, flavonoids and phenolic acids fractions were obtained using flash and open column chromatography. The main compounds gentisic acid, ferulic acid, gallic acid, p-coumaric acid, sinapic acid, 4-hydroxybenzoic acid, delphinidin, cyanidin, vanillic acid, delphinidin gallate, gallocatechin gallate, quercetin, rutin, myricetin, catechin and epi-catechin as mixture 1:1, and several glycosides of anthocyanidins (delphinidin-3-sambubioside-5-glucoside, delphinidin-3,5-diglucoside, cyanidin-3-sambubioside-5-glucoside, cyanidin-3,5-diglucoside, delphinidin-3-sambubioside, delphinidin-3-glucoside, cyanidin-3-sambubioside, and cyanidin-3-glucoside), and proanthocyanidin B were detected. In addition to phytochemical analysis the antioxidant activities of extracts, partitions and fractions were strongly correlated with the highest polyphenol contents. The most active samples were the ethanolic and acetone extracts in all bioassays used and all samples were compared for activity against butylated hydroxy toluene (BHT), quercetin and tocopherol used as pattern samples. The juice (E), EtOH extract (A) and acetone partition (B) were found to have IC50 values of 4.7, 1.7 and 7.4 ppm, respectively against DPPH and 5.9, 2.1 and 3.9 ppm, respectively against TBARS formation. Additionally, the fraction F-4 showed a strong activity with IC50 of 4.9 and 6.5 ppm, against DPPH and TBARS respectively. Consistent with this finding, EtOH extract had the greatest ORAC and FRAP values as percentage of activity. On the other hand the IC50 values for the inhibitory activity against O 2 - of extract B, F-3 and F-4 were 9.7, 13.2 and 10.7 ppm, respectively and against OH − were 29.1, 7.0 and 6.3 ppm, respectively. The EtOH extract protects against stress oxidative reducing the concentration of the MDA a lipid peroxidation index. These results shows that this fruit could be useful as antioxidant and nutraceutical sources.