Thiobarbituric Acid

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Kevin Robards - One of the best experts on this subject based on the ideXlab platform.

  • liquid chromatography mass spectrometry lc ms investigation of the Thiobarbituric Acid reactive substances tbars reaction
    Journal of Agricultural and Food Chemistry, 2002
    Co-Authors: Daniel Jardine, Michael Antolovich, Paul D Prenzle, Kevin Robards
    Abstract:

    The Thiobarbituric Acid reactive substances (TBARS) assay is a commonly used method for the detection of lipid peroxidation. Malondialdehyde is formed as a result of lipid peroxidation and reacts with Thiobarbituric Acid to form a pink pigment that has an absorption maximum at 532 nm. Other compounds also react with Thiobarbituric Acid to form colored species that can interfere with this assay, but little is known about these interfering species. This is the first investigation using LC-MS and MS-MS to study the structures of the pink adduct as well as a common unstable yellow interference compound, which absorbs at 455 nm. Also, the presence of barbituric Acid impurities in the Thiobarbituric Acid reagent was found to produce 1:1:1 Thiobarbituric Acid/malondialdehyde/barbituric Acid and 2:1 barbituric Acid/malondialdehyde adducts that absorbed at 513 and 490 nm, respectively, indicating that Thiobarbituric Acid should be purified before use. Keywords: TBARS; mass spectrometry; interferences

  • Liquid chromatography-mass spectrometry (LC-MS) investigation of the Thiobarbituric Acid reactive substances (TBARS) reaction.
    Journal of Agricultural and Food Chemistry, 2002
    Co-Authors: Daniel Jardine, Michael Antolovich, Paul D. Prenzler, Kevin Robards
    Abstract:

    The Thiobarbituric Acid reactive substances (TBARS) assay is a commonly used method for the detection of lipid peroxidation. Malondialdehyde is formed as a result of lipid peroxidation and reacts with Thiobarbituric Acid to form a pink pigment that has an absorption maximum at 532 nm. Other compounds also react with Thiobarbituric Acid to form colored species that can interfere with this assay, but little is known about these interfering species. This is the first investigation using LC-MS and MS-MS to study the structures of the pink adduct as well as a common unstable yellow interference compound, which absorbs at 455 nm. Also, the presence of barbituric Acid impurities in the Thiobarbituric Acid reagent was found to produce 1:1:1 Thiobarbituric Acid/malondialdehyde/barbituric Acid and 2:1 barbituric Acid/malondialdehyde adducts that absorbed at 513 and 490 nm, respectively, indicating that Thiobarbituric Acid should be purified before use.

Daniel Jardine - One of the best experts on this subject based on the ideXlab platform.

  • liquid chromatography mass spectrometry lc ms investigation of the Thiobarbituric Acid reactive substances tbars reaction
    Journal of Agricultural and Food Chemistry, 2002
    Co-Authors: Daniel Jardine, Michael Antolovich, Paul D Prenzle, Kevin Robards
    Abstract:

    The Thiobarbituric Acid reactive substances (TBARS) assay is a commonly used method for the detection of lipid peroxidation. Malondialdehyde is formed as a result of lipid peroxidation and reacts with Thiobarbituric Acid to form a pink pigment that has an absorption maximum at 532 nm. Other compounds also react with Thiobarbituric Acid to form colored species that can interfere with this assay, but little is known about these interfering species. This is the first investigation using LC-MS and MS-MS to study the structures of the pink adduct as well as a common unstable yellow interference compound, which absorbs at 455 nm. Also, the presence of barbituric Acid impurities in the Thiobarbituric Acid reagent was found to produce 1:1:1 Thiobarbituric Acid/malondialdehyde/barbituric Acid and 2:1 barbituric Acid/malondialdehyde adducts that absorbed at 513 and 490 nm, respectively, indicating that Thiobarbituric Acid should be purified before use. Keywords: TBARS; mass spectrometry; interferences

  • Liquid chromatography-mass spectrometry (LC-MS) investigation of the Thiobarbituric Acid reactive substances (TBARS) reaction.
    Journal of Agricultural and Food Chemistry, 2002
    Co-Authors: Daniel Jardine, Michael Antolovich, Paul D. Prenzler, Kevin Robards
    Abstract:

    The Thiobarbituric Acid reactive substances (TBARS) assay is a commonly used method for the detection of lipid peroxidation. Malondialdehyde is formed as a result of lipid peroxidation and reacts with Thiobarbituric Acid to form a pink pigment that has an absorption maximum at 532 nm. Other compounds also react with Thiobarbituric Acid to form colored species that can interfere with this assay, but little is known about these interfering species. This is the first investigation using LC-MS and MS-MS to study the structures of the pink adduct as well as a common unstable yellow interference compound, which absorbs at 455 nm. Also, the presence of barbituric Acid impurities in the Thiobarbituric Acid reagent was found to produce 1:1:1 Thiobarbituric Acid/malondialdehyde/barbituric Acid and 2:1 barbituric Acid/malondialdehyde adducts that absorbed at 513 and 490 nm, respectively, indicating that Thiobarbituric Acid should be purified before use.

Antonios G. Trakatellis - One of the best experts on this subject based on the ideXlab platform.

  • Rapid, Sensitive, and Specific Thiobarbituric Acid Method for Measuring Lipid Peroxidation in Animal Tissue, Food, and Feedstuff Samples
    Journal of Agricultural and Food Chemistry, 1994
    Co-Authors: Nickos A Botsoglou, D.j. Fletouris, Georgios E. Papageorgiou, Vassilios N. Vassilopoulos, Antonios J. Mantis, Antonios G. Trakatellis
    Abstract:

    A rapid aqueous Acid extraction Thiobarbituric Acid method for measuring malondialdehyde as a marker of lipid peroxidation in animal tissue, food, and feedstuff samples has been developed. Sample is homogenized with aqueous trichloroacetic Acid in the presence of hexane and butylated hydroxytoluene, and the homogenate is centrifuged. Following reaction with Thiobarbituric Acid reagent, malondialdehyde is directly quantified on the basis of the third-derivative absorption spectrum of the pink complex formed. Further purification is not required because the derivative transformation of the conventional analytical band at around 532 nm virtually eliminates spectral interferences arising from other compounds. The effect of antioxidants and the optimum conditions for the reaction have been established, and the analytical performance of the new method has been evaluated. The applicability of the method on various animal tissue, food, and feedstuff samples has been also tested. Owing to its simplicity and increased sensitivity and specificity, the method may be preferred over other methods for estimating the extent of lipid peroxidation.

Michael Antolovich - One of the best experts on this subject based on the ideXlab platform.

  • liquid chromatography mass spectrometry lc ms investigation of the Thiobarbituric Acid reactive substances tbars reaction
    Journal of Agricultural and Food Chemistry, 2002
    Co-Authors: Daniel Jardine, Michael Antolovich, Paul D Prenzle, Kevin Robards
    Abstract:

    The Thiobarbituric Acid reactive substances (TBARS) assay is a commonly used method for the detection of lipid peroxidation. Malondialdehyde is formed as a result of lipid peroxidation and reacts with Thiobarbituric Acid to form a pink pigment that has an absorption maximum at 532 nm. Other compounds also react with Thiobarbituric Acid to form colored species that can interfere with this assay, but little is known about these interfering species. This is the first investigation using LC-MS and MS-MS to study the structures of the pink adduct as well as a common unstable yellow interference compound, which absorbs at 455 nm. Also, the presence of barbituric Acid impurities in the Thiobarbituric Acid reagent was found to produce 1:1:1 Thiobarbituric Acid/malondialdehyde/barbituric Acid and 2:1 barbituric Acid/malondialdehyde adducts that absorbed at 513 and 490 nm, respectively, indicating that Thiobarbituric Acid should be purified before use. Keywords: TBARS; mass spectrometry; interferences

  • Liquid chromatography-mass spectrometry (LC-MS) investigation of the Thiobarbituric Acid reactive substances (TBARS) reaction.
    Journal of Agricultural and Food Chemistry, 2002
    Co-Authors: Daniel Jardine, Michael Antolovich, Paul D. Prenzler, Kevin Robards
    Abstract:

    The Thiobarbituric Acid reactive substances (TBARS) assay is a commonly used method for the detection of lipid peroxidation. Malondialdehyde is formed as a result of lipid peroxidation and reacts with Thiobarbituric Acid to form a pink pigment that has an absorption maximum at 532 nm. Other compounds also react with Thiobarbituric Acid to form colored species that can interfere with this assay, but little is known about these interfering species. This is the first investigation using LC-MS and MS-MS to study the structures of the pink adduct as well as a common unstable yellow interference compound, which absorbs at 455 nm. Also, the presence of barbituric Acid impurities in the Thiobarbituric Acid reagent was found to produce 1:1:1 Thiobarbituric Acid/malondialdehyde/barbituric Acid and 2:1 barbituric Acid/malondialdehyde adducts that absorbed at 513 and 490 nm, respectively, indicating that Thiobarbituric Acid should be purified before use.

Ashok Kumar - One of the best experts on this subject based on the ideXlab platform.

  • synthesis of some newer derivatives of substituted quinazolinonyl 2 oxo Thiobarbituric Acid as potent anticonvulsant agents
    Bioorganic & Medicinal Chemistry, 2004
    Co-Authors: V K Srivastava, Ashok Kumar
    Abstract:

    Abstract 5-{1′-[3″-Aminoacetyl-2″-methyl-6″,8″-dihalosubstitutedquinazolin-4″(3″H)-onyl]-thiosemicarbazido}-2-oxo/Thiobarbituric Acids 3a – 3h and 5-{2′-amino-5′-[3″-aminomethylene-2″-methyl-6″,8″-dihalosubstitutedquinazolin-4″(3″H)-onyl]-1′,3′,4′-thiadiazol-2′-yl}-2-oxo/Thiobarbituric Acid 5a – 5h were prepared by incorporating 1-[3′-aminoacetyl-2′-methyl-6″,8″-dihalosubstituted-quinazolin-4′(3′H)-onyl]-thiosemicarbazides 2a – 2d and 2-amino-5-[3′-aminomethylene-2′-methyl-6′,8′-dihalosubstituted-quinazolin-4′(3′H)-onyl]-1,3,4-thiadiazoles 4a-4 h respectively at 5 th position of 2-oxo/Thiobarbituric Acids (via Mannich reaction). All the newly synthesized compounds were screened for their anti-convulsant activity in MES and PTZ models and were compared with standard drugs phenytoin sodium and sodium valproate. Interestingly, these compounds were found to be devoid of sedative and hypnotic activities when tested. Out of the compounds studied, the most active compound 5h , that is 5-{2′-amino-5′-[3″-aminomethylene-2″-methyl-6″,8″-dibromoquinazolin-4″(3″H)-onyl]-1′,3′,4′-thiadiazol-2′-yl}-2-Thiobarbituric Acid showed activity (90%) more potent than the standard drug.

  • Synthesis of some newer derivatives of substituted quinazolinonyl-2-oxo/Thiobarbituric Acid as potent anticonvulsant agents.
    Bioorganic & Medicinal Chemistry, 2004
    Co-Authors: Archana, Vishnu Srivastava, Ashok Kumar
    Abstract:

    Abstract 5-{1′-[3″-Aminoacetyl-2″-methyl-6″,8″-dihalosubstitutedquinazolin-4″(3″H)-onyl]-thiosemicarbazido}-2-oxo/Thiobarbituric Acids 3a – 3h and 5-{2′-amino-5′-[3″-aminomethylene-2″-methyl-6″,8″-dihalosubstitutedquinazolin-4″(3″H)-onyl]-1′,3′,4′-thiadiazol-2′-yl}-2-oxo/Thiobarbituric Acid 5a – 5h were prepared by incorporating 1-[3′-aminoacetyl-2′-methyl-6″,8″-dihalosubstituted-quinazolin-4′(3′H)-onyl]-thiosemicarbazides 2a – 2d and 2-amino-5-[3′-aminomethylene-2′-methyl-6′,8′-dihalosubstituted-quinazolin-4′(3′H)-onyl]-1,3,4-thiadiazoles 4a-4 h respectively at 5 th position of 2-oxo/Thiobarbituric Acids (via Mannich reaction). All the newly synthesized compounds were screened for their anti-convulsant activity in MES and PTZ models and were compared with standard drugs phenytoin sodium and sodium valproate. Interestingly, these compounds were found to be devoid of sedative and hypnotic activities when tested. Out of the compounds studied, the most active compound 5h , that is 5-{2′-amino-5′-[3″-aminomethylene-2″-methyl-6″,8″-dibromoquinazolin-4″(3″H)-onyl]-1′,3′,4′-thiadiazol-2′-yl}-2-Thiobarbituric Acid showed activity (90%) more potent than the standard drug.