The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
Robert H Edwards - One of the best experts on this subject based on the ideXlab platform.
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the mechanism and regulation of vesicular glutamate transport coordination with the synaptic vesicle cycle
Biochimica et Biophysica Acta, 2020Co-Authors: Jacob Eriksen, Fei Li, Robert H EdwardsAbstract:The transport of classical neurotransmitters into synaptic vesicles generally relies on a H+ electrochemical gradient (∆μH+). Synaptic vesicle uptake of glutamate depends primarily on the Electrical Component ∆ψ as the driving force, rather than the chemical Component ∆pH. However, the vesicular glutamate transporters (VGLUTs) belong to the solute carrier 17 (SLC17) family, which includes closely related members that function as H+ cotransporters. Recent work has also shown that the VGLUTs undergo allosteric regulation by H+ and Cl-, and exhibit an associated Cl- conductance. These properties appear to coordinate VGLUT activity with the large ionic shifts that accompany the rapid recycling of synaptic vesicles driven by neural activity. Recent structural information also suggests common mechanisms that underlie the apparently divergent function of SLC17 family members, and that confer allosteric regulation.
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Presynaptic regulation of quantal size: K^+/H^+ exchange stimulates vesicular glutamate transport
Nature Neuroscience, 2011Co-Authors: Germaine Y Goh, Hai Huang, Julie Ullman, Lars Borre, Thomas S Hnasko, Laurence O Trussell, Robert H EdwardsAbstract:The authors report that rat brain glutamatergic synaptic vesicles express monovalent cation/H^+ exchangers that convert the Δψ of the proton electrochemical gradient into Δψ. They find that this K^+/H^+ exchange stimulates the accumulation of glutamate into vesicles, regulating glutamate release and thus synaptic transmission. The amount of neurotransmitter stored in a single synaptic vesicle can determine the size of the postsynaptic response, but the factors that regulate vesicle filling are poorly understood. A proton electrochemical gradient (Δμ_H+) generated by the vacuolar H^+-ATPase drives the accumulation of classical transmitters into synaptic vesicles. The chemical Component of Δμ_H+ (ΔpH) has received particular attention for its role in the vesicular transport of cationic transmitters as well as in protein sorting and degradation. Thus, considerable work has addressed the factors that promote ΔpH. However, synaptic vesicle uptake of the principal excitatory transmitter glutamate depends on the Electrical Component of Δμ_H+ (Δψ). We found that rat brain synaptic vesicles express monovalent cation/H^+ exchange activity that converts ΔpH into Δψ, and that this promotes synaptic vesicle filling with glutamate. Manipulating presynaptic K^+ at a glutamatergic synapse influenced quantal size, indicating that synaptic vesicle K^+/H^+ exchange regulates glutamate release and synaptic transmission.
Jacob Eriksen - One of the best experts on this subject based on the ideXlab platform.
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the mechanism and regulation of vesicular glutamate transport coordination with the synaptic vesicle cycle
Biochimica et Biophysica Acta, 2020Co-Authors: Jacob Eriksen, Fei Li, Robert H EdwardsAbstract:The transport of classical neurotransmitters into synaptic vesicles generally relies on a H+ electrochemical gradient (∆μH+). Synaptic vesicle uptake of glutamate depends primarily on the Electrical Component ∆ψ as the driving force, rather than the chemical Component ∆pH. However, the vesicular glutamate transporters (VGLUTs) belong to the solute carrier 17 (SLC17) family, which includes closely related members that function as H+ cotransporters. Recent work has also shown that the VGLUTs undergo allosteric regulation by H+ and Cl-, and exhibit an associated Cl- conductance. These properties appear to coordinate VGLUT activity with the large ionic shifts that accompany the rapid recycling of synaptic vesicles driven by neural activity. Recent structural information also suggests common mechanisms that underlie the apparently divergent function of SLC17 family members, and that confer allosteric regulation.
Dragana Jovanovic - One of the best experts on this subject based on the ideXlab platform.
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GdVO4:Er3+/Yb3+ nanocrystalline powder as fluorescence temperature sensor. Application to monitor the temperature of an Electrical Component
Sensors and Actuators A: Physical, 2019Co-Authors: Franzette Paz-buclatin, F. Rivera-lópez, O. Gonzalez, Inocencio R. Martín, Leopoldo L. Martin, Dragana JovanovicAbstract:Abstract The temperature sensing properties of an Er3+/Yb3+ co-doped GdVO4 nanocrystalline powder were studied. The down-conversion emission spectrum of the sample was observed under excitation at 457 nm. Two methods were used to calibrate the temperature of the sample: one based on the Fluorescence Intensity Ratio (FIR) technique and the other using the fluorescence lifetime of the thermally-coupled energy levels of Er3+. The relative sensitivities for each method were calculated and it was found out that the FIR-based temperature sensor has higher sensitivity (1.17% K−1) than the lifetime-based sensor (0.24% K−1). Furthermore, a temperature uncertainty of 0.37 K was obtained for the FIR-based sensor. The GdVO4 nanoparticles were also used to study the change in temperature of an Electrical Component when it is operating and not.
Budi Susilo - One of the best experts on this subject based on the ideXlab platform.
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Development of Maintenance-Reparation Guidance, and Material Specification for Green Building’s Electrical Component in Government Building Based on Work Breakdown Structure (WBS)
IOP Conference Series: Materials Science and Engineering, 2018Co-Authors: Resha Mochamad Ilham, Yusuf Latief, Leni Sagita Riantini, Budi SusiloAbstract:Initial quality of building depands on quality of design and construction phase of the building, while maintenance and spare part quality keep the decline of the quality during operational phase of building within level of service needed. Electrical aspect of building has an impact of the building's purpose, safety, and comfort. Several finding in operation phase have been found regarding lack of quality of Electrical maintenance process and spare part material such as fire incident, waste of electricity and exesive stock of spare part. Thus, it's important to not only develop maintenance and reparation guidelines of Electrical work but also it's material specification to keep the required level of service. The purpose of this study is to use work breakdown structure (WBS) methods as a proven method in decomposition to develop maintenance and reparation guidelines, as well as the material specifications in green building. The research methodology covers several stages: literature studies, information from previous projects, and delphi techniques through expert validation in green building Electrical Components. The results of this study are expected to create applicable guidelines for maintenance and reparation work of Electrical Component and it's material specification to eliminate or decrease finding of Electrical issues in building operational.
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development of maintenance reparation guidance and material specification for green building s Electrical Component in government building based on work breakdown structure wbs
IOP Conference Series: Materials Science and Engineering, 2018Co-Authors: Resha Mochamad Ilham, Yusuf Latief, Leni Sagita Riantini, Budi SusiloAbstract:Initial quality of building depands on quality of design and construction phase of the building, while maintenance and spare part quality keep the decline of the quality during operational phase of building within level of service needed. Electrical aspect of building has an impact of the building's purpose, safety, and comfort. Several finding in operation phase have been found regarding lack of quality of Electrical maintenance process and spare part material such as fire incident, waste of electricity and exesive stock of spare part. Thus, it's important to not only develop maintenance and reparation guidelines of Electrical work but also it's material specification to keep the required level of service. The purpose of this study is to use work breakdown structure (WBS) methods as a proven method in decomposition to develop maintenance and reparation guidelines, as well as the material specifications in green building. The research methodology covers several stages: literature studies, information from previous projects, and delphi techniques through expert validation in green building Electrical Components. The results of this study are expected to create applicable guidelines for maintenance and reparation work of Electrical Component and it's material specification to eliminate or decrease finding of Electrical issues in building operational.
Germaine Y Goh - One of the best experts on this subject based on the ideXlab platform.
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Presynaptic regulation of quantal size: K^+/H^+ exchange stimulates vesicular glutamate transport
Nature Neuroscience, 2011Co-Authors: Germaine Y Goh, Hai Huang, Julie Ullman, Lars Borre, Thomas S Hnasko, Laurence O Trussell, Robert H EdwardsAbstract:The authors report that rat brain glutamatergic synaptic vesicles express monovalent cation/H^+ exchangers that convert the Δψ of the proton electrochemical gradient into Δψ. They find that this K^+/H^+ exchange stimulates the accumulation of glutamate into vesicles, regulating glutamate release and thus synaptic transmission. The amount of neurotransmitter stored in a single synaptic vesicle can determine the size of the postsynaptic response, but the factors that regulate vesicle filling are poorly understood. A proton electrochemical gradient (Δμ_H+) generated by the vacuolar H^+-ATPase drives the accumulation of classical transmitters into synaptic vesicles. The chemical Component of Δμ_H+ (ΔpH) has received particular attention for its role in the vesicular transport of cationic transmitters as well as in protein sorting and degradation. Thus, considerable work has addressed the factors that promote ΔpH. However, synaptic vesicle uptake of the principal excitatory transmitter glutamate depends on the Electrical Component of Δμ_H+ (Δψ). We found that rat brain synaptic vesicles express monovalent cation/H^+ exchange activity that converts ΔpH into Δψ, and that this promotes synaptic vesicle filling with glutamate. Manipulating presynaptic K^+ at a glutamatergic synapse influenced quantal size, indicating that synaptic vesicle K^+/H^+ exchange regulates glutamate release and synaptic transmission.