The Experts below are selected from a list of 1995 Experts worldwide ranked by ideXlab platform
Joseph Wang - One of the best experts on this subject based on the ideXlab platform.
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In situ Electrochemical Monitoring: from remote sensors to submersible microlaboratories
Laboratory Robotics and Automation, 2020Co-Authors: Joseph WangAbstract:This article reviews new concepts for in situ environmental analysis based on novel Electrochemical sensing strategies. Several new strategies are covered, including the use of remotely deployed sensors and submersible (“lab-on-cable”) analyzers. These devices offer a fast return of the chemical information in a safe and timely manner. The new concepts are discussed in connection to the in situ Monitoring of priority organic and inorganic contaminants. Such devices should have an enormous impact upon pollution control and prevention, because they should lead to a substantially more effective and economic Monitoring of priority pollutants. © 2000 John Wiley & Sons, Inc. Lab Robotics and Automation 12:178–182, 2000
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real time Electrochemical Monitoring of drug release from therapeutic nanoparticles
Journal of Controlled Release, 2009Co-Authors: Laura Mora, Karin Y Chumbimunitorres, Corbin Clawson, L Hernandez, Liangfang Zhang, Joseph WangAbstract:An Electrochemical protocol for real-time Monitoring of drug release kinetics from therapeutic nanoparticles (NPs) is described. The method is illustrated for repetitive square-wave voltammetric measurements of the reduction of doxorubicin released from liposomes at a glassy-carbon electrode. Such operation couples high sensitivity down to 20 nM doxorubicin with high speed and stability. It can thus monitor in real time the drug release from NP carriers, including continuous measurements in diluted serum. Such direct and continuous Monitoring of the drug release kinetics from therapeutic NPs holds great promise for designing new drug delivery NPs with optimal drug release properties. These NPs can potentially be used to deliver many novel compounds such as marine-life derived drugs and hydrophobic drugs with limited water solubility that are usually difficult to be characterized by traditional analytical tools.
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Direct Electrochemical Monitoring of RNase Activity
Electroanalysis, 2008Co-Authors: Yongkang Ye, Yun Xiang, Xiaodong Qi, Jeffrey T. La Belle, Julian J.-l. Chen, Joseph WangAbstract:Here we present a highly sensitive, rapid and simple Electrochemical assay of RNase based on coupling magnetic separation of the enzymatically treated RNA with stripping potentiometric detection of the purine nucleobases. A detection limit of 1 � 10 � 8 U RNase (ca. 4 pg/mL) is obtained in connection to a 60 min enzymatic digestion. The attractive performance of this direct indicator-free Electrochemical assay offers great promise for a wide range of molecular biology and water quality applications.
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real time Electrochemical Monitoring toward green analytical chemistry
Accounts of Chemical Research, 2002Co-Authors: Joseph WangAbstract:This Account presents a survey of recent advances in Electrochemical sensing technology relevant to green analytical chemistry and examines the potential advantages, limitations, and applications of these Monitoring devices. Stricter environmental control and effective process Monitoring have created considerable demands for innovative analytical methodologies. New devices and protocols, with negligible waste generation or no hazardous substances, and in situ real-time Monitoring capability are particularly needed for addressing the challenges of green analytical chemistry. The coupling of modern Electrochemical detection principles with recent advances in molecular recognition, microelectronics, and microfabrication has led to powerful, compact, and “user-friendly” analytical devices. The unique features of such Electrochemical Monitoring systems make them particularly attractive for addressing environmental and industrial problems and the challenges of green chemistry. These developments allow the instr...
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Real-time Electrochemical Monitoring: Toward green analytical chemistry
Accounts of Chemical Research, 2002Co-Authors: Joseph WangAbstract:This Account presents a survey of recent advances in Electrochemical sensing technology relevant to green analytical chemistry and examines the potential advantages, limitations, and applications of these Monitoring devices. Stricter environmental control and effective process Monitoring have created considerable demands for innovative analytical methodologies. New devices and protocols, with negligible waste generation or no hazardous substances, and in situ real-time Monitoring capability are particularly needed for addressing the challenges of green analytical chemistry. The coupling of modern Electrochemical detection principles with recent advances in molecular recognition, microelectronics, and microfabrication has led to powerful, compact, and "user-friendly" analytical devices. The unique features of such Electrochemical Monitoring systems make them particularly attractive for addressing environmental and industrial problems and the challenges of green chemistry. These developments allow the instrument to be taken to the sample (rather than the traditional way of bringing the sample to the laboratory) and hence to ensure effective process or pollution control.
Mohammed Zourob - One of the best experts on this subject based on the ideXlab platform.
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high throughput real time Electrochemical Monitoring of lamp for pathogenic bacteria detection
Biosensors and Bioelectronics, 2014Co-Authors: Mohammadali Safavieh, Minhaz Uddin Ahmed, Andy Ng, Mohammed ZourobAbstract:abstract One of the significant challenges in healthcare is the development of point-of-care (POC) diagnostics.POC diagnostics require low-cost devices that offer portability, simplicity in operation and the ability forhigh-throughput and quantitative analysis. Here, we present a novel roll-to-roll ribbon fluid-handlingdevice for Electrochemical real-time Monitoring of nucleic acid (NA) amplification and bacteria detection.The device rendered loop-mediated isothermal amplification (LAMP) and real-time Electrochemicaldetection based on the interaction between LAMP amplicon and the redox-reactive osmium complex.We have shown the detection of 30 CFU/ml of Escherichia coli (in the range between 30 and 3 710 CFU/ml) and 200 CFU/ml of Staphylococcus aureus (in the range of 200–2 10 5 CFU/ml) cultured samples inboth real-time and end point detection. This device can be used for the detection of various Gram-negative and a number of Gram-positive bacterial pathogens with high sensitivity and specificity in ahigh-throughput format. Using a roll-to-roll cassette approach, we could detect 12 samples in one assay.Since the LAMP and Electrochemical analysis are implemented within sealed flexible biochips, time-consuming processing steps are not required and the risk of contamination is significantly reduced.& 2014 Elsevier B.V. All rights reserved.
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High-throughput real-time Electrochemical Monitoring of LAMP for pathogenic bacteria detection
Biosensors and Bioelectronics, 2014Co-Authors: Mohammadali Safavieh, Minhaz Uddin Ahmed, Andy Ng, Mohammed ZourobAbstract:One of the significant challenges in healthcare is the development of point-of-care (POC) diagnostics. POC diagnostics require low-cost devices that offer portability, simplicity in operation and the ability for high-throughput and quantitative analysis. Here, we present a novel roll-to-roll ribbon fluid-handling device for Electrochemical real-time Monitoring of nucleic acid (NA) amplification and bacteria detection. The device rendered loop-mediated isothermal amplification (LAMP) and real-time Electrochemical detection based on the interaction between LAMP amplicon and the redox-reactive osmium complex. We have shown the detection of 30CFU/ml of Escherichia coli (in the range between 30 and 3×107CFU/ml) and 200CFU/ml of Staphylococcus aureus (in the range of 200-2×105CFU/ml) cultured samples in both real-time and end point detection. This device can be used for the detection of various Gram-negative and a number of Gram-positive bacterial pathogens with high sensitivity and specificity in a high-throughput format. Using a roll-to-roll cassette approach, we could detect 12 samples in one assay. Since the LAMP and Electrochemical analysis are implemented within sealed flexible biochips, time-consuming processing steps are not required and the risk of contamination is significantly reduced. © 2014 Elsevier B.V.
Arzum Erdem - One of the best experts on this subject based on the ideXlab platform.
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Carbon quantum dot modified electrodes developed for Electrochemical Monitoring of Daunorubicin-DNA interaction
Journal of Electroanalytical Chemistry, 2020Co-Authors: Ece Eksin, Haluk Bingol, Huseyin Senturk, Arzum ErdemAbstract:Abstract The carbon quantum dot (cQD) modified disposable pencil graphite electrodes were developed for the first time in this study for Electrochemical Monitoring of drug-DNA interaction. The biomolecular interaction between calf thymus double stranded DNA (ctDNA) and an anthracycline antineoplastic drug, Daunorubicin (DNR) was investigated by differential pulse voltammetry (DPV) technique. For this purpose, experimental conditions, such as, the concentration of ctDNA, concentration of DNR and interaction time were optimized. Under optimum conditions, the detection limits of DNR and ctDNA were found to be 0.02 μg/mL and 0.89 μg/mL, respectively. The effect of interaction time between DNR and ctDNA was explored upon to the changes at both DNR and guanine oxidation signals. Drug-DNA interaction process was also examined by Electrochemical impedance spectroscopy (EIS) technique.
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Chitosan modified graphite electrodes developed for Electrochemical Monitoring of interaction between daunorubicin and DNA
Sensing and bio-sensing research, 2019Co-Authors: Gulsah Congur, Ece Eksin, Arzum ErdemAbstract:Abstract Chitosan (CHIT) modified single-use electrodes were developed for the first time in the present study and applied for Electrochemical Monitoring of anticancer drug-DNA interaction. Under this aim, pencil graphite electrode (PGE) was used as the biosensor platform and the modification of PGEs using biopolymer, CHIT was performed by passive adsorption process. Microscopic and Electrochemical characterizations of CHIT modified PGEs were performed. An anticancer drug, Daunorubicin (DNR) was analyzed by using both PGEs and CHIT-PGEs. Moreover, the effect of CHIT modification on biosensor development upon to the sensitivity of voltammetric detection of DNR and DNA was also investigated. The interaction between DNA and DNR was then performed and accordingly, the voltammetric measurements were performed before/after interaction process by using differential pulse voltammetry (DPV) technique.
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Electrochemical Monitoring of biointeraction by graphene-based material modified pencil graphite electrode.
Biosensors and Bioelectronics, 2017Co-Authors: Ece Eksin, Arzum Erdem, Haluk BingolAbstract:Abstract Recently, the low-cost effective biosensing systems based on advanced nanomaterials have received a key attention for development of novel assays for rapid and sequence-specific nucleic acid detection. The Electrochemical biosensor based on reduced graphene oxide (rGO) modified disposable pencil graphite electrodes (PGEs) were developed herein for Electrochemical Monitoring of DNA, and also for Monitoring of biointeraction occurred between anticancer drug, Daunorubicin (DNR), and DNA. First, rGO was synthesized chemically and characterized by using UV–Vis, TGA, FT-IR, Raman Spectroscopy and SEM techniques. Then, the quantity of rGO assembling onto the surface of PGE by passive adsorption was optimized. The Electrochemical behavior of rGO–PGEs was examined by cyclic voltammetry (CV). rGO-PGEs were then utilized for Electrochemical Monitoring of surface-confined interaction between DNR and DNA using differential pulse voltammetry (DPV) technique. Additionally, voltammetric results were complemented with Electrochemical impedance spectroscopy (EIS) technique. Electrochemical Monitoring of DNR and DNA was resulted with satisfying detection limits 0.55 µM and 2.71 µg/mL, respectively.
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Multiwalled Carbon Nanotubes-Chitosan Modified Single-Use Biosensors for Electrochemical Monitoring of Drug-DNA Interactions
Electroanalysis, 2015Co-Authors: Ceren Sengiz, Ece Eksin, Gulsah Congur, Arzum ErdemAbstract:A multiwalled carbon nanotubes (CNT)-chitosan (CHIT) modified pencil graphite electrode (CNT-CHIT/PGE) was developed for the first time herein for Electrochemical Monitoring of the interaction of an anticancer drug, mitomycin C (MC) and DNA. The characterization of unmodified PGE, CHIT/PGE, CNT/PGE and CHIT-CNT/PGE were performed by scanning electron microscopy and cyclic voltammetry techniques. The oxidation signals of MC and guanine were measured before and after interaction at the surface of CNT-CHIT/PGEs using differential pulse voltammetry. Electrochemical impedance spectroscopy technique was also successfully utilized for Monitoring of the interaction process at the surface of CNT-CHIT/PGEs in different interaction times.
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Electrochemical Monitoring of surface confined interaction between 6-Thioguanine and DNA by using single-use graphite electrode
Journal of Electroanalytical Chemistry, 2014Co-Authors: Ece Eksin, Gulsah Congur, Fehmi Mese, Arzum ErdemAbstract:Abstract In the present work, the Electrochemical detection of interaction between 6-TG and calf thymus double stranded DNA (dsDNA) was explored by using single-use pencil graphite electrode (PGE) in combination with differential pulse voltammetry (DPV) and Electrochemical impedance spectroscopy (EIS) techniques. Firstly, the Electrochemical behaviour of 6-TG was investigated voltammetrically by using PGE, and the two oxidation signals of 6-TG were measured at +0.25 V and +0.78 V. Experimental parameters, such as 6-TG concentration and interaction time were optimized based on the changes at the 6-TG signal observed at +0.25 V since the latter one overlapped with the oxidation signal of guanine measured at +0.95 V. The results based on the impedimetric analysis for interaction between 6-TG and dsDNA were consistent with the voltammetric results. The Electrochemical Monitoring of surface confined interaction between 6-TG and single stranded Oligo (A) 25 , or double stranded Oligo (A) 25 –Oligo (T) 25 was explored based on the changes at the oxidation signals of 6-TG and adenine by using DPV. The single-use graphite sensor represented a sensitive, rapid and cost effective detection protocol for Electrochemical Monitoring of the interaction between 6-TG and DNA.
Laura Mora - One of the best experts on this subject based on the ideXlab platform.
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real time Electrochemical Monitoring of drug release from therapeutic nanoparticles
Journal of Controlled Release, 2009Co-Authors: Laura Mora, Karin Y Chumbimunitorres, Corbin Clawson, L Hernandez, Liangfang Zhang, Joseph WangAbstract:An Electrochemical protocol for real-time Monitoring of drug release kinetics from therapeutic nanoparticles (NPs) is described. The method is illustrated for repetitive square-wave voltammetric measurements of the reduction of doxorubicin released from liposomes at a glassy-carbon electrode. Such operation couples high sensitivity down to 20 nM doxorubicin with high speed and stability. It can thus monitor in real time the drug release from NP carriers, including continuous measurements in diluted serum. Such direct and continuous Monitoring of the drug release kinetics from therapeutic NPs holds great promise for designing new drug delivery NPs with optimal drug release properties. These NPs can potentially be used to deliver many novel compounds such as marine-life derived drugs and hydrophobic drugs with limited water solubility that are usually difficult to be characterized by traditional analytical tools.
Minhaz Uddin Ahmed - One of the best experts on this subject based on the ideXlab platform.
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high throughput real time Electrochemical Monitoring of lamp for pathogenic bacteria detection
Biosensors and Bioelectronics, 2014Co-Authors: Mohammadali Safavieh, Minhaz Uddin Ahmed, Andy Ng, Mohammed ZourobAbstract:abstract One of the significant challenges in healthcare is the development of point-of-care (POC) diagnostics.POC diagnostics require low-cost devices that offer portability, simplicity in operation and the ability forhigh-throughput and quantitative analysis. Here, we present a novel roll-to-roll ribbon fluid-handlingdevice for Electrochemical real-time Monitoring of nucleic acid (NA) amplification and bacteria detection.The device rendered loop-mediated isothermal amplification (LAMP) and real-time Electrochemicaldetection based on the interaction between LAMP amplicon and the redox-reactive osmium complex.We have shown the detection of 30 CFU/ml of Escherichia coli (in the range between 30 and 3 710 CFU/ml) and 200 CFU/ml of Staphylococcus aureus (in the range of 200–2 10 5 CFU/ml) cultured samples inboth real-time and end point detection. This device can be used for the detection of various Gram-negative and a number of Gram-positive bacterial pathogens with high sensitivity and specificity in ahigh-throughput format. Using a roll-to-roll cassette approach, we could detect 12 samples in one assay.Since the LAMP and Electrochemical analysis are implemented within sealed flexible biochips, time-consuming processing steps are not required and the risk of contamination is significantly reduced.& 2014 Elsevier B.V. All rights reserved.
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High-throughput real-time Electrochemical Monitoring of LAMP for pathogenic bacteria detection
Biosensors and Bioelectronics, 2014Co-Authors: Mohammadali Safavieh, Minhaz Uddin Ahmed, Andy Ng, Mohammed ZourobAbstract:One of the significant challenges in healthcare is the development of point-of-care (POC) diagnostics. POC diagnostics require low-cost devices that offer portability, simplicity in operation and the ability for high-throughput and quantitative analysis. Here, we present a novel roll-to-roll ribbon fluid-handling device for Electrochemical real-time Monitoring of nucleic acid (NA) amplification and bacteria detection. The device rendered loop-mediated isothermal amplification (LAMP) and real-time Electrochemical detection based on the interaction between LAMP amplicon and the redox-reactive osmium complex. We have shown the detection of 30CFU/ml of Escherichia coli (in the range between 30 and 3×107CFU/ml) and 200CFU/ml of Staphylococcus aureus (in the range of 200-2×105CFU/ml) cultured samples in both real-time and end point detection. This device can be used for the detection of various Gram-negative and a number of Gram-positive bacterial pathogens with high sensitivity and specificity in a high-throughput format. Using a roll-to-roll cassette approach, we could detect 12 samples in one assay. Since the LAMP and Electrochemical analysis are implemented within sealed flexible biochips, time-consuming processing steps are not required and the risk of contamination is significantly reduced. © 2014 Elsevier B.V.