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Shouzhuo Yao - One of the best experts on this subject based on the ideXlab platform.
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simultaneous analysis and identification of main bioactive constituents in extract of zizyphus jujuba var sapinosa zizyphi spinosi semen by high performance liquid chromatography photodiode array detection Electrospray Mass Spectrometry
Talanta, 2007Co-Authors: Jingjing Liu, Bo Chen, Shouzhuo YaoAbstract:A high-performance liquid chromatography (HPLC) coupled with UV photodiode array detection and Electrospray Mass Spectrometry (HPLC-DAD-ESI-MS) was developed for the simultaneous analysis and identification of main bioactive constituents in extract of zizyphi spinosi semen including three flavonoids, three alkaloids and four triterpene saponins. The separation was achieved on a C(18) column with a gradient solvent system of 0.08% aqueous formic acid solution-acetonitrile. The compounds were identified with MS-spectra and authentic standards. The contents of jujuboside A, jujuboside B, spinosin and 6'''-feruloyl spinosin were quantified by using DAD in different extract of zizyphi spinosi semen.
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simultaneous determination of water soluble and fat soluble synthetic colorants in foodstuff by high performance liquid chromatography diode array detection Electrospray Mass Spectrometry
Journal of Chromatography A, 2006Co-Authors: Xubiao Luo, Bo Chen, Shouzhuo YaoAbstract:An accurate method was developed for the simultaneous determination of water-Tartrazine, Amaranth, Ponceau 4R, Sunset Yellow FCF, and fat-Sudan (I-IV), synthetic soluble colorants in foodstuff. This method uses dimethylsulfoxide (DMSO) as the extraction solvent in the sample preparation process and high performance liquid chromatography (HPLC)-diode array detector (DAD)-Electrospray Mass Spectrometry (ESI-MS), applying selected ion recording in positive/negative alternate mode to acquire Mass spectral data, as the analytical technique. Linearity of around three orders in the magnitude of concentration was generally obtained. Detection and quantification limits of the investigated dyes, which were evaluated at signal to noise ratio of 3 for detection limit and 10 for quantification limit, were in the ranges of 0.01-4 and 0.03-11.2 ng, respectively. The recoveries of the eight synthetic colorants in four matrices ranged from 93.2 to 108.3%. Relative standard deviations of less than 8.2% were also achieved. This method has been applied successfully in the determination of water-soluble colorants in the soft drink and the delicious ginger, and fat-soluble dyes in chilli powders and chilli spices.
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simultaneous analysis of n nornuciferine o nornuciferine nuciferine and roemerine in leaves of nelumbo nucifera gaertn by high performance liquid chromatography photodiode array detection Electrospray Mass Spectrometry
Analytica Chimica Acta, 2005Co-Authors: Xubiao Luo, Bo Chen, Jingjing Liu, Shouzhuo YaoAbstract:Abstract A novel method has been developed for analysis of N -nornuciferine, O -nornuciferine, nuciferine, and roemerine in leaves of Nelumbo nucifera Gaertn by using high-performance liquid chromatography (HPLC)–photodiode array detection (DAD)–Electrospray Mass Spectrometry (ESI-MS). The method was carried out by using a Shimadzu VP-ODS column with a gradient solvent system of 0.1% triethylamine aqueous solution–acetonitrile. N -Nornuciferine, O -nornuciferine, nuciferine, and roemerine were identified with authentic standard compounds and with MS-spectra. The contents of these compounds were measured by employing DAD. Linearity of around three orders in the magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 30–90 pg.
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simultaneous determination of benazepril hydrochloride and benazeprilat in plasma by high performance liquid chromatography Electrospray Mass Spectrometry
Journal of Chromatography B, 2005Co-Authors: Weitao Xiao, Bo Chen, Shouzhuo Yao, Zeneng ChengAbstract:An analytical method for simultaneous determination of benazepril and its active metabolite, benazeprilat, in human plasma by high-performance liquid chromatography/Electrospray-Mass Spectrometry was developed and validated. Rutaecarpine was selected as the internal standard. The separation was achieved on a C(18) column with acetonitrile and aqueous solution (0.1% formic acid) as mobile phase with a gradient mode. The quantification of target compounds was using a selective ionization recording at m/z 425.5 for benazepril, m/z 397.5 for benzeprilat and m/z 288.3 for rutaecarpine. The correlation coefficients of the calibration curves were better than 0.992 (n = 6), in the range of 6.67-666.67 ng/ml for benazepril and benazeprilat. The inter- and intra-day accuracy, precision, linear range had been investigated in detail. The method can be used to assess the bioavailability and pharmacokinetics of the drug.
Bo Chen - One of the best experts on this subject based on the ideXlab platform.
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simultaneous analysis and identification of main bioactive constituents in extract of zizyphus jujuba var sapinosa zizyphi spinosi semen by high performance liquid chromatography photodiode array detection Electrospray Mass Spectrometry
Talanta, 2007Co-Authors: Jingjing Liu, Bo Chen, Shouzhuo YaoAbstract:A high-performance liquid chromatography (HPLC) coupled with UV photodiode array detection and Electrospray Mass Spectrometry (HPLC-DAD-ESI-MS) was developed for the simultaneous analysis and identification of main bioactive constituents in extract of zizyphi spinosi semen including three flavonoids, three alkaloids and four triterpene saponins. The separation was achieved on a C(18) column with a gradient solvent system of 0.08% aqueous formic acid solution-acetonitrile. The compounds were identified with MS-spectra and authentic standards. The contents of jujuboside A, jujuboside B, spinosin and 6'''-feruloyl spinosin were quantified by using DAD in different extract of zizyphi spinosi semen.
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simultaneous determination of water soluble and fat soluble synthetic colorants in foodstuff by high performance liquid chromatography diode array detection Electrospray Mass Spectrometry
Journal of Chromatography A, 2006Co-Authors: Xubiao Luo, Bo Chen, Shouzhuo YaoAbstract:An accurate method was developed for the simultaneous determination of water-Tartrazine, Amaranth, Ponceau 4R, Sunset Yellow FCF, and fat-Sudan (I-IV), synthetic soluble colorants in foodstuff. This method uses dimethylsulfoxide (DMSO) as the extraction solvent in the sample preparation process and high performance liquid chromatography (HPLC)-diode array detector (DAD)-Electrospray Mass Spectrometry (ESI-MS), applying selected ion recording in positive/negative alternate mode to acquire Mass spectral data, as the analytical technique. Linearity of around three orders in the magnitude of concentration was generally obtained. Detection and quantification limits of the investigated dyes, which were evaluated at signal to noise ratio of 3 for detection limit and 10 for quantification limit, were in the ranges of 0.01-4 and 0.03-11.2 ng, respectively. The recoveries of the eight synthetic colorants in four matrices ranged from 93.2 to 108.3%. Relative standard deviations of less than 8.2% were also achieved. This method has been applied successfully in the determination of water-soluble colorants in the soft drink and the delicious ginger, and fat-soluble dyes in chilli powders and chilli spices.
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simultaneous determination of water soluble and fat soluble synthetic colorants in foodstuff by high performance liquid chromatography diode array detection Electrospray Mass Spectrometry
Journal of Chromatography A, 2006Co-Authors: Bo Chen, Shengpei SuAbstract:An accurate method was developed for the simultaneous determination of water-Tartrazine, Amaranth, Ponceau 4R, Sunset Yellow FCF, and fat-Sudan (I–IV), synthetic soluble colorants in foodstuff. This method uses dimethylsulfoxide (DMSO) as the extraction solvent in the sample preparation process and high performance liquid chromatography (HPLC)–diode array detector (DAD)–Electrospray Mass Spectrometry (ESI-MS), applying selected ion recording in positive/negative alternate mode to acquire Mass spectral data, as the analytical technique. Linearity of aroun d three orders in the magnitude of concentration was generally obtained. Detection and quantification limits of the investigated dyes, which were evaluated at signal to noise ratio of 3 for detection limit and 10 for quantification limit, were in the ranges of 0.01–4 and 0.03–11.2 ng, respectively. The recoveries of the eight synthetic colorants in four matrices ranged from 93.2 to 108.3%. Relative standard deviations of less than 8.2% were also achieved. This method has been applied successfully in the determination of water-soluble colorants in the soft drink and the delicious ginger, and fat-soluble dyes in chilli powders and chilli spices. © 2005 Elsevier B.V. All rights reserved.
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simultaneous analysis of n nornuciferine o nornuciferine nuciferine and roemerine in leaves of nelumbo nucifera gaertn by high performance liquid chromatography photodiode array detection Electrospray Mass Spectrometry
Analytica Chimica Acta, 2005Co-Authors: Xubiao Luo, Bo Chen, Jingjing Liu, Shouzhuo YaoAbstract:Abstract A novel method has been developed for analysis of N -nornuciferine, O -nornuciferine, nuciferine, and roemerine in leaves of Nelumbo nucifera Gaertn by using high-performance liquid chromatography (HPLC)–photodiode array detection (DAD)–Electrospray Mass Spectrometry (ESI-MS). The method was carried out by using a Shimadzu VP-ODS column with a gradient solvent system of 0.1% triethylamine aqueous solution–acetonitrile. N -Nornuciferine, O -nornuciferine, nuciferine, and roemerine were identified with authentic standard compounds and with MS-spectra. The contents of these compounds were measured by employing DAD. Linearity of around three orders in the magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 30–90 pg.
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simultaneous analysis of protoberberine indolequinoline and quinolone alkaloids in coptis evodia herb couple and the chinese herbal preparations by high performance liquid chromatography Electrospray Mass Spectrometry
Talanta, 2005Co-Authors: Bo ChenAbstract:A new and sensitive method has been developed for analysis of protoberberine, indolequinoline and quinolone alkaloids in coptis–evodia herb couple and the Chinese herbal preparations by using HPLC in combination with Electrospray Mass Spectrometry (ESI-MS). Different LC and cone voltages have been optimized prior to this in order to obtain better results and sensitivity. The method was carried out by using a spherigel C18 analytical column with a gradient solvent system of acetate buffer–acetonitrile–methanol. The contents of alkaloids were measured by pseudomolecule ion on the selective ion recording (SIR) mode. Linearity of around three orders in the magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 9–30 pg. © 2004 Elsevier B.V. All rights reserved.
Xubiao Luo - One of the best experts on this subject based on the ideXlab platform.
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simultaneous determination of water soluble and fat soluble synthetic colorants in foodstuff by high performance liquid chromatography diode array detection Electrospray Mass Spectrometry
Journal of Chromatography A, 2006Co-Authors: Xubiao Luo, Bo Chen, Shouzhuo YaoAbstract:An accurate method was developed for the simultaneous determination of water-Tartrazine, Amaranth, Ponceau 4R, Sunset Yellow FCF, and fat-Sudan (I-IV), synthetic soluble colorants in foodstuff. This method uses dimethylsulfoxide (DMSO) as the extraction solvent in the sample preparation process and high performance liquid chromatography (HPLC)-diode array detector (DAD)-Electrospray Mass Spectrometry (ESI-MS), applying selected ion recording in positive/negative alternate mode to acquire Mass spectral data, as the analytical technique. Linearity of around three orders in the magnitude of concentration was generally obtained. Detection and quantification limits of the investigated dyes, which were evaluated at signal to noise ratio of 3 for detection limit and 10 for quantification limit, were in the ranges of 0.01-4 and 0.03-11.2 ng, respectively. The recoveries of the eight synthetic colorants in four matrices ranged from 93.2 to 108.3%. Relative standard deviations of less than 8.2% were also achieved. This method has been applied successfully in the determination of water-soluble colorants in the soft drink and the delicious ginger, and fat-soluble dyes in chilli powders and chilli spices.
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simultaneous analysis of n nornuciferine o nornuciferine nuciferine and roemerine in leaves of nelumbo nucifera gaertn by high performance liquid chromatography photodiode array detection Electrospray Mass Spectrometry
Analytica Chimica Acta, 2005Co-Authors: Xubiao Luo, Bo Chen, Jingjing Liu, Shouzhuo YaoAbstract:Abstract A novel method has been developed for analysis of N -nornuciferine, O -nornuciferine, nuciferine, and roemerine in leaves of Nelumbo nucifera Gaertn by using high-performance liquid chromatography (HPLC)–photodiode array detection (DAD)–Electrospray Mass Spectrometry (ESI-MS). The method was carried out by using a Shimadzu VP-ODS column with a gradient solvent system of 0.1% triethylamine aqueous solution–acetonitrile. N -Nornuciferine, O -nornuciferine, nuciferine, and roemerine were identified with authentic standard compounds and with MS-spectra. The contents of these compounds were measured by employing DAD. Linearity of around three orders in the magnitude of concentration was generally obtained and limits of detection for these compounds were in the range of 30–90 pg.
John Crosby - One of the best experts on this subject based on the ideXlab platform.
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a comparison of deconvolution methods as applied to high performance liquid chromatography diode array detector Electrospray Mass Spectrometry of 2 and 3 hydroxypyridine at varying ph in the presence of severely tailing peak shapes
Chemometrics and Intelligent Laboratory Systems, 1999Co-Authors: Samantha Dunkerley, Richard G Brereton, John CrosbyAbstract:Abstract Triply coupled high performance liquid chromatography–diode array detection–Electrospray Mass Spectrometry of a mixture of 2- and 3-hydroxypyridine were recorded at pH 5.0, 5.1 and 5.2. At each pH the resolution changes with almost complete overlap at pH 5.1. A variety of chemometric methods including raw, normalised and standardised principal component analysis (PCA), derivatives, evolving factor analysis (EFA), fixed size window EFA, spectral similarity indices (SSI) and peak ratios are applied to the data for both detectors and all pH. Methods of deconvolution are also applied to both the diode array and Mass spectral data.
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chemometric analysis of high performance liquid chromatography diode array detection Electrospray Mass Spectrometry of 2 and 3 hydroxypyridine
Analyst, 1998Co-Authors: Samantha Dunkerley, John Crosby, Richard G Brereton, Konstantinos D Zissis, Richard E A EscottAbstract:Triply coupled high performance liquid chromatography using diode array detection and positive ion Electrospray Mass Spectrometry of 2- and 3-hydroxypyridine is presented. Considerations of the physical method for coupling the two detectors, the influence of pH on retention times, the cone voltage of the Mass spectrometer and the linear concentration ranges are described. Data from both detectors are aligned and interpolated. The analyte Mass spectra are reduced to 20 significant Masses. Principal components plots on the raw, normalised and standardised data, derivatives to determine composition 1 regions, deconvolution and procrustes analysis to compare data from both detectors are discussed. Common trends in both Mass spectral and diode array chromatograms are interpreted. This paper represents a new approach to common processing of chromatographic data from two detectors.
Samantha Dunkerley - One of the best experts on this subject based on the ideXlab platform.
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a comparison of deconvolution methods as applied to high performance liquid chromatography diode array detector Electrospray Mass Spectrometry of 2 and 3 hydroxypyridine at varying ph in the presence of severely tailing peak shapes
Chemometrics and Intelligent Laboratory Systems, 1999Co-Authors: Samantha Dunkerley, Richard G Brereton, John CrosbyAbstract:Abstract Triply coupled high performance liquid chromatography–diode array detection–Electrospray Mass Spectrometry of a mixture of 2- and 3-hydroxypyridine were recorded at pH 5.0, 5.1 and 5.2. At each pH the resolution changes with almost complete overlap at pH 5.1. A variety of chemometric methods including raw, normalised and standardised principal component analysis (PCA), derivatives, evolving factor analysis (EFA), fixed size window EFA, spectral similarity indices (SSI) and peak ratios are applied to the data for both detectors and all pH. Methods of deconvolution are also applied to both the diode array and Mass spectral data.
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chemometric analysis of high performance liquid chromatography diode array detection Electrospray Mass Spectrometry of 2 and 3 hydroxypyridine
Analyst, 1998Co-Authors: Samantha Dunkerley, John Crosby, Richard G Brereton, Konstantinos D Zissis, Richard E A EscottAbstract:Triply coupled high performance liquid chromatography using diode array detection and positive ion Electrospray Mass Spectrometry of 2- and 3-hydroxypyridine is presented. Considerations of the physical method for coupling the two detectors, the influence of pH on retention times, the cone voltage of the Mass spectrometer and the linear concentration ranges are described. Data from both detectors are aligned and interpolated. The analyte Mass spectra are reduced to 20 significant Masses. Principal components plots on the raw, normalised and standardised data, derivatives to determine composition 1 regions, deconvolution and procrustes analysis to compare data from both detectors are discussed. Common trends in both Mass spectral and diode array chromatograms are interpreted. This paper represents a new approach to common processing of chromatographic data from two detectors.