The Experts below are selected from a list of 234 Experts worldwide ranked by ideXlab platform
Clive P Duncan - One of the best experts on this subject based on the ideXlab platform.
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Elution characteristics of vancomycin and tobramycin combined in acrylic bone cement
Journal of Arthroplasty, 1996Co-Authors: Murray J Penner, Bassam A Masri, Clive P DuncanAbstract:Abstract Combining two antibiotics in antibiotic-loaded bone-cement is common in clinical practice. As the effect this has on Elution characteristics is unknown, an in vivo quantitative Elution study was carried out. Three groups of five antibiotic-loaded cement disks were prepared and placed in individual saline baths for 5 weeks. The Elution of tobramycin from the disks in the study group (containing 2.4 g tobramycin and 1.0 g vancomycin per 40-g packet of Palacos-R cement powder [Smith & Nephew Orthopaedics, Memphis, TN]) was increased by 68% over that of the tobramycin control disks (2.4 g tobramycin only) ( P = .024). The release of vancomycin from the study group disks was increased by 103% over the vancomycin control disks (1.0 g vancomycin only) ( P = .007). Combining two antibiotics in bone—cement improves Elution of both antibiotics in vitro and may translate into enhanced Elution in vivo .
Francesco La Torre - One of the best experts on this subject based on the ideXlab platform.
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high performance liquid chromatography enantioseparation of proton pump inhibitors using the immobilized amylose based chiralpak ia chiral stationary phase in normal phase polar organic and reversed phase conditions
Journal of Chromatography A, 2008Co-Authors: Roberto Cirilli, Rosella Ferretti, Bruno Gallinella, Emiliana De Santis, Leo Zanitti, Francesco La TorreAbstract:The chiral resolving ability of the amylose-based Chiralpak IA chiral stationary phase towards omeprazole and other proton pump inhibitors under reversed-phase conditions was investigated. Organic modifier-buffer demonstrated to be a valid alternative Elution mode with respect to conventional polar organic and normal-phases. No evidence of deterioration of performance of the enantioselective column after several multimodal cycles of Elution was observed. Mobile phase composition was systematically changed in order to modulate the enantiomer Elution order of set of compounds studied. A very simple method based on on-line detection of optical rotational sign during enantioselective HPLC was developed to assign the absolute configuration and enantiomeric Elution order.
Murray J Penner - One of the best experts on this subject based on the ideXlab platform.
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Elution characteristics of vancomycin and tobramycin combined in acrylic bone cement
Journal of Arthroplasty, 1996Co-Authors: Murray J Penner, Bassam A Masri, Clive P DuncanAbstract:Abstract Combining two antibiotics in antibiotic-loaded bone-cement is common in clinical practice. As the effect this has on Elution characteristics is unknown, an in vivo quantitative Elution study was carried out. Three groups of five antibiotic-loaded cement disks were prepared and placed in individual saline baths for 5 weeks. The Elution of tobramycin from the disks in the study group (containing 2.4 g tobramycin and 1.0 g vancomycin per 40-g packet of Palacos-R cement powder [Smith & Nephew Orthopaedics, Memphis, TN]) was increased by 68% over that of the tobramycin control disks (2.4 g tobramycin only) ( P = .024). The release of vancomycin from the study group disks was increased by 103% over the vancomycin control disks (1.0 g vancomycin only) ( P = .007). Combining two antibiotics in bone—cement improves Elution of both antibiotics in vitro and may translate into enhanced Elution in vivo .
Takeshi Tomonaga - One of the best experts on this subject based on the ideXlab platform.
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improved proteome and phosphoproteome analysis on a cation exchanger by a combined acid and salt gradient
Analytical Chemistry, 2016Co-Authors: Jun Adachi, Kazunari Hashiguchi, Maiko Nagano, Misako Sato, Ayako Sato, Kazuna Fukamizu, Yasushi Ishihama, Takeshi TomonagaAbstract:Currently used Elution methods for strong cation exchange (SCX) chromatography are based on two principles: salt and pH gradient. In this paper, we report the first observation of peptide Elution by acid gradient. The degree of peptide separation using C18-SCX StageTip was greatly improved by our acid and salt-based Elution method compared with a salt-based Elution method. This development enabled us to identify over 22 000 phosphopeptides from 2 mg of protein without labor-intensive sample preparation. Our method is simple, robust, scalable, and low-cost and can be easily implemented without any special equipment or techniques.
Roberto Cirilli - One of the best experts on this subject based on the ideXlab platform.
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high performance liquid chromatography enantioseparation of proton pump inhibitors using the immobilized amylose based chiralpak ia chiral stationary phase in normal phase polar organic and reversed phase conditions
Journal of Chromatography A, 2008Co-Authors: Roberto Cirilli, Rosella Ferretti, Bruno Gallinella, Emiliana De Santis, Leo Zanitti, Francesco La TorreAbstract:The chiral resolving ability of the amylose-based Chiralpak IA chiral stationary phase towards omeprazole and other proton pump inhibitors under reversed-phase conditions was investigated. Organic modifier-buffer demonstrated to be a valid alternative Elution mode with respect to conventional polar organic and normal-phases. No evidence of deterioration of performance of the enantioselective column after several multimodal cycles of Elution was observed. Mobile phase composition was systematically changed in order to modulate the enantiomer Elution order of set of compounds studied. A very simple method based on on-line detection of optical rotational sign during enantioselective HPLC was developed to assign the absolute configuration and enantiomeric Elution order.