The Experts below are selected from a list of 213 Experts worldwide ranked by ideXlab platform

Pingdong Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Embryo Sac chromosome doubling in populus alba p glandulosa induced by high temperature exposure to produce triploids
    Breeding Science, 2017
    Co-Authors: Yanjie Li, Mengdi Tian, Pingdong Zhang
    Abstract:

    To determine the effects of the hours after pollination and the treatment durations on triploid production and reveal the effective stages of Embryo Sac chromosome doubling by high temperature exposure. At least three catkins were sampled, and 80 ovules were used for the determination of the Embryo Sac developmental process. Catkins (2-74 h after pollination) were treated to induce Embryo Sac chromosome doubling. Cytological observations revealed that the Embryo Sac development was a consecutive and asynchronous process. Fertilization occurred 50 h after pollination. In the offspring seedlings, 167 triploids were detected and the highest efficiency of triploid production was 87.0%. Among all the induced triploids, the most effective treatment period of inducing Embryo Sac chromosome doubling is from 26 to 50 h after pollination, and 121 triploids were obtained, representing 72.46% of the sum of all triploids. GLM-Univariate analysis indicated significant differences among the hours after pollination (F = 4.516, p = 0.045). However, the differences between the treatment durations (F = 0.077, p = 0.791) were not significant. Correlation analysis between the proportion of each Embryo Sac's developmental stage and the percentage of triploid production indicated that the third mitotic division may be the most effective stage for 2n female gamete induction.

  • Embryo Sac chromosome doubling in Populus alba × P. glandulosa induced by high temperature exposure to produce triploids
    Breeding Science, 2017
    Co-Authors: Yanjie Li, Mengdi Tian, Pingdong Zhang
    Abstract:

    To determine the effects of the hours after pollination and the treatment durations on triploid production and reveal the effective stages of Embryo Sac chromosome doubling by high temperature exposure. At least three catkins were sampled, and 80 ovules were used for the determination of the Embryo Sac developmental process. Catkins (2-74 h after pollination) were treated to induce Embryo Sac chromosome doubling. Cytological observations revealed that the Embryo Sac development was a consecutive and asynchronous process. Fertilization occurred 50 h after pollination. In the offspring seedlings, 167 triploids were detected and the highest efficiency of triploid production was 87.0%. Among all the induced triploids, the most effective treatment period of inducing Embryo Sac chromosome doubling is from 26 to 50 h after pollination, and 121 triploids were obtained, representing 72.46% of the sum of all triploids. GLM-Univariate analysis indicated significant differences among the hours after pollination (F = 4.516, p = 0.045). However, the differences between the treatment durations (F = 0.077, p = 0.791) were not significant. Correlation analysis between the proportion of each Embryo Sac's developmental stage and the percentage of triploid production indicated that the third mitotic division may be the most effective stage for 2n female gamete induction.

Xian Sheng Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Transcript profiles of maize Embryo Sacs and preliminary identification of genes involved in the Embryo Sac–pollen tube interaction
    Frontiers in plant science, 2014
    Co-Authors: Shuai Shuai Wang, Fang Wang, Su Jian Tan, Ming Xiu Wang, Na Sui, Xian Sheng Zhang
    Abstract:

    The Embryo Sac, the female gametophyte of flowering plants, plays important roles in the pollination and fertilization process. Maize (Zea mays L.) is a model monocot, but little is known about the interactions between its Embryo Sac and the pollen tube. In this study, we compared the transcript profiles of mature Embryo Sacs, mature Embryo Sacs 14–16 h after pollination, and mature nucelli. Comparing the transcript profiles of the Embryo Sac before and after the entry of the pollen tube, we identified 3,467 differentially expressed transcripts (3,382 differentially expressed genes; DEGs). The DEGs were grouped into 22 functional categories. Among the DEGs, 221genes were induced upon the entry of the pollen tube, and many of them encoded proteins involved in RNA binding, processing, and transcription, signaling, miscellaneous enzyme family processes, and lipid metabolism processes. Genes in the DEG dataset were grouped into 17 classes in a gene ontology enrichment analysis. The DEGs included many genes encoding proteins involved in protein amino acid phosphorylation and protein ubiquitination, implying that these processes might play important roles in the Embryo Sac–pollen tube interaction. Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization. The CRPs likely regulate pollen tube guidance and Embryo Sac development. These results provide important information on the genes involved in the Embryo Sac–pollen tube interaction in maize.

  • transcript profiles of maize Embryo Sacs and preliminary identification of genes involved in the Embryo Sac pollen tube interaction
    Frontiers in Plant Science, 2014
    Co-Authors: Shuai Shuai Wang, Fang Wang, Su Jian Tan, Ming Xiu Wang, Na Sui, Xian Sheng Zhang
    Abstract:

    The Embryo Sac, the female gametophyte of flowering plants, plays important roles in the pollination and fertilization process. Maize (Zea mays L.) is a model monocot, but little is known about the interactions between its Embryo Sac and the pollen tube. In this study, we compared the transcript profiles of mature Embryo Sacs, mature Embryo Sacs 14–16 h after pollination, and mature nucelli. Comparing the transcript profiles of the Embryo Sac before and after the entry of the pollen tube, we identified 3,467 differentially expressed transcripts (3,382 differentially expressed genes; DEGs). The DEGs were grouped into 22 functional categories. Among the DEGs, 221genes were induced upon the entry of the pollen tube, and many of them encoded proteins involved in RNA binding, processing, and transcription, signaling, miscellaneous enzyme family processes, and lipid metabolism processes. Genes in the DEG dataset were grouped into 17 classes in a gene ontology enrichment analysis. The DEGs included many genes encoding proteins involved in protein amino acid phosphorylation and protein ubiquitination, implying that these processes might play important roles in the Embryo Sac–pollen tube interaction. Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization. The CRPs likely regulate pollen tube guidance and Embryo Sac development. These results provide important information on the genes involved in the Embryo Sac–pollen tube interaction in maize.

Yanjie Li - One of the best experts on this subject based on the ideXlab platform.

  • Embryo Sac chromosome doubling in populus alba p glandulosa induced by high temperature exposure to produce triploids
    Breeding Science, 2017
    Co-Authors: Yanjie Li, Mengdi Tian, Pingdong Zhang
    Abstract:

    To determine the effects of the hours after pollination and the treatment durations on triploid production and reveal the effective stages of Embryo Sac chromosome doubling by high temperature exposure. At least three catkins were sampled, and 80 ovules were used for the determination of the Embryo Sac developmental process. Catkins (2-74 h after pollination) were treated to induce Embryo Sac chromosome doubling. Cytological observations revealed that the Embryo Sac development was a consecutive and asynchronous process. Fertilization occurred 50 h after pollination. In the offspring seedlings, 167 triploids were detected and the highest efficiency of triploid production was 87.0%. Among all the induced triploids, the most effective treatment period of inducing Embryo Sac chromosome doubling is from 26 to 50 h after pollination, and 121 triploids were obtained, representing 72.46% of the sum of all triploids. GLM-Univariate analysis indicated significant differences among the hours after pollination (F = 4.516, p = 0.045). However, the differences between the treatment durations (F = 0.077, p = 0.791) were not significant. Correlation analysis between the proportion of each Embryo Sac's developmental stage and the percentage of triploid production indicated that the third mitotic division may be the most effective stage for 2n female gamete induction.

  • Embryo Sac chromosome doubling in Populus alba × P. glandulosa induced by high temperature exposure to produce triploids
    Breeding Science, 2017
    Co-Authors: Yanjie Li, Mengdi Tian, Pingdong Zhang
    Abstract:

    To determine the effects of the hours after pollination and the treatment durations on triploid production and reveal the effective stages of Embryo Sac chromosome doubling by high temperature exposure. At least three catkins were sampled, and 80 ovules were used for the determination of the Embryo Sac developmental process. Catkins (2-74 h after pollination) were treated to induce Embryo Sac chromosome doubling. Cytological observations revealed that the Embryo Sac development was a consecutive and asynchronous process. Fertilization occurred 50 h after pollination. In the offspring seedlings, 167 triploids were detected and the highest efficiency of triploid production was 87.0%. Among all the induced triploids, the most effective treatment period of inducing Embryo Sac chromosome doubling is from 26 to 50 h after pollination, and 121 triploids were obtained, representing 72.46% of the sum of all triploids. GLM-Univariate analysis indicated significant differences among the hours after pollination (F = 4.516, p = 0.045). However, the differences between the treatment durations (F = 0.077, p = 0.791) were not significant. Correlation analysis between the proportion of each Embryo Sac's developmental stage and the percentage of triploid production indicated that the third mitotic division may be the most effective stage for 2n female gamete induction.

Shuai Shuai Wang - One of the best experts on this subject based on the ideXlab platform.

  • Transcript profiles of maize Embryo Sacs and preliminary identification of genes involved in the Embryo Sac–pollen tube interaction
    Frontiers in plant science, 2014
    Co-Authors: Shuai Shuai Wang, Fang Wang, Su Jian Tan, Ming Xiu Wang, Na Sui, Xian Sheng Zhang
    Abstract:

    The Embryo Sac, the female gametophyte of flowering plants, plays important roles in the pollination and fertilization process. Maize (Zea mays L.) is a model monocot, but little is known about the interactions between its Embryo Sac and the pollen tube. In this study, we compared the transcript profiles of mature Embryo Sacs, mature Embryo Sacs 14–16 h after pollination, and mature nucelli. Comparing the transcript profiles of the Embryo Sac before and after the entry of the pollen tube, we identified 3,467 differentially expressed transcripts (3,382 differentially expressed genes; DEGs). The DEGs were grouped into 22 functional categories. Among the DEGs, 221genes were induced upon the entry of the pollen tube, and many of them encoded proteins involved in RNA binding, processing, and transcription, signaling, miscellaneous enzyme family processes, and lipid metabolism processes. Genes in the DEG dataset were grouped into 17 classes in a gene ontology enrichment analysis. The DEGs included many genes encoding proteins involved in protein amino acid phosphorylation and protein ubiquitination, implying that these processes might play important roles in the Embryo Sac–pollen tube interaction. Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization. The CRPs likely regulate pollen tube guidance and Embryo Sac development. These results provide important information on the genes involved in the Embryo Sac–pollen tube interaction in maize.

  • transcript profiles of maize Embryo Sacs and preliminary identification of genes involved in the Embryo Sac pollen tube interaction
    Frontiers in Plant Science, 2014
    Co-Authors: Shuai Shuai Wang, Fang Wang, Su Jian Tan, Ming Xiu Wang, Na Sui, Xian Sheng Zhang
    Abstract:

    The Embryo Sac, the female gametophyte of flowering plants, plays important roles in the pollination and fertilization process. Maize (Zea mays L.) is a model monocot, but little is known about the interactions between its Embryo Sac and the pollen tube. In this study, we compared the transcript profiles of mature Embryo Sacs, mature Embryo Sacs 14–16 h after pollination, and mature nucelli. Comparing the transcript profiles of the Embryo Sac before and after the entry of the pollen tube, we identified 3,467 differentially expressed transcripts (3,382 differentially expressed genes; DEGs). The DEGs were grouped into 22 functional categories. Among the DEGs, 221genes were induced upon the entry of the pollen tube, and many of them encoded proteins involved in RNA binding, processing, and transcription, signaling, miscellaneous enzyme family processes, and lipid metabolism processes. Genes in the DEG dataset were grouped into 17 classes in a gene ontology enrichment analysis. The DEGs included many genes encoding proteins involved in protein amino acid phosphorylation and protein ubiquitination, implying that these processes might play important roles in the Embryo Sac–pollen tube interaction. Additionally, our analyses indicate that the expression of 112 genes encoding cysteine-rich proteins (CRPs) is induced during pollination and fertilization. The CRPs likely regulate pollen tube guidance and Embryo Sac development. These results provide important information on the genes involved in the Embryo Sac–pollen tube interaction in maize.

Mengdi Tian - One of the best experts on this subject based on the ideXlab platform.

  • Embryo Sac chromosome doubling in populus alba p glandulosa induced by high temperature exposure to produce triploids
    Breeding Science, 2017
    Co-Authors: Yanjie Li, Mengdi Tian, Pingdong Zhang
    Abstract:

    To determine the effects of the hours after pollination and the treatment durations on triploid production and reveal the effective stages of Embryo Sac chromosome doubling by high temperature exposure. At least three catkins were sampled, and 80 ovules were used for the determination of the Embryo Sac developmental process. Catkins (2-74 h after pollination) were treated to induce Embryo Sac chromosome doubling. Cytological observations revealed that the Embryo Sac development was a consecutive and asynchronous process. Fertilization occurred 50 h after pollination. In the offspring seedlings, 167 triploids were detected and the highest efficiency of triploid production was 87.0%. Among all the induced triploids, the most effective treatment period of inducing Embryo Sac chromosome doubling is from 26 to 50 h after pollination, and 121 triploids were obtained, representing 72.46% of the sum of all triploids. GLM-Univariate analysis indicated significant differences among the hours after pollination (F = 4.516, p = 0.045). However, the differences between the treatment durations (F = 0.077, p = 0.791) were not significant. Correlation analysis between the proportion of each Embryo Sac's developmental stage and the percentage of triploid production indicated that the third mitotic division may be the most effective stage for 2n female gamete induction.

  • Embryo Sac chromosome doubling in Populus alba × P. glandulosa induced by high temperature exposure to produce triploids
    Breeding Science, 2017
    Co-Authors: Yanjie Li, Mengdi Tian, Pingdong Zhang
    Abstract:

    To determine the effects of the hours after pollination and the treatment durations on triploid production and reveal the effective stages of Embryo Sac chromosome doubling by high temperature exposure. At least three catkins were sampled, and 80 ovules were used for the determination of the Embryo Sac developmental process. Catkins (2-74 h after pollination) were treated to induce Embryo Sac chromosome doubling. Cytological observations revealed that the Embryo Sac development was a consecutive and asynchronous process. Fertilization occurred 50 h after pollination. In the offspring seedlings, 167 triploids were detected and the highest efficiency of triploid production was 87.0%. Among all the induced triploids, the most effective treatment period of inducing Embryo Sac chromosome doubling is from 26 to 50 h after pollination, and 121 triploids were obtained, representing 72.46% of the sum of all triploids. GLM-Univariate analysis indicated significant differences among the hours after pollination (F = 4.516, p = 0.045). However, the differences between the treatment durations (F = 0.077, p = 0.791) were not significant. Correlation analysis between the proportion of each Embryo Sac's developmental stage and the percentage of triploid production indicated that the third mitotic division may be the most effective stage for 2n female gamete induction.