The Experts below are selected from a list of 219 Experts worldwide ranked by ideXlab platform
Gbo Guilherme Benfatti Olivato - One of the best experts on this subject based on the ideXlab platform.
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p448infective endocarditis in patent ductus arteriosus by bartonella sp case report and literature review
Cardiovascular Research, 2014Co-Authors: Gbo Guilherme Benfatti Olivato, Frf Fernando Roberto De Fazzio, Tms Thaysa Moreira Santos, Mrp Milena Ribeiro Paixao, Wfg Wilton Francisco Gomes, Drgd Daniel Renato Goncalves Duarte, Tjgt Thais Juliano Garcia Tosta, Nmi Nana Miura IkariAbstract:Case report: APS, 19 years, female, outpatients with a diagnosis of patent ductus arteriosus in planning surgical correction sought emergency room presenting episodes of fever measured 2 weeks ago, asthenia and fatigue. Additional research showed pancytopenia and hepatosplenomegaly. Prompted transthoracic echocardiography showing orifice of the ductus arteriosus thicked image hiperrefringente, mobile, looks filamentous pedunculated measuring 13 mm in length, the topography of the ductus arteriosus, pulmonary attached to the end. Requested three pairs of blood cultures without detectable bacterial growth. Patient admitted for investigation and treatment of infective Endarteritis probable subacute seronegative blood channel, and also request surveys by indirect immunofluorescence for Bartonella sp, with positive results. Patient admitted for treatment with antibiotics and surgery scheduled for correction of patent ductus arteriosus, and started intravenous penicillin and gentamicin. During admission had septic symptomatic pulmonary embolization, followed by emergency surgery for correction of patent ductus arteriosus. Favorable outcome after high end drug treatment for 28 days. Discussion: Bartonella sp is a common cause of infectious endocarditis seronegative, with few reports in the literature. However, there are no reports in the literature National endarterites ductus arteriosus caused by such bacteria. Since this diagnosis is considered difficult at the beginning of the investigation, it should search request Bartonella sp in all cases of infective endocarditis and Endarteritis in patent ductus ateriosus with negative blood cultures, since the prevalence of this microorganism has been increasing in Brazil.
David J. Kerr - One of the best experts on this subject based on the ideXlab platform.
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Small bowel fistula secondary to Endarteritis obliterans, complicating chemotherapy for small bowel lymphoma.
The British journal of radiology, 1992Co-Authors: S. Knowles, H. E. Smart, A. Al-dawoud, David J. KerrAbstract:Endarteritis obliterans, pathological thickening of the intima associated with luminal stenosis of small and medium sized arteries, is a relatively common late tissue effect following radiotherapy, but it is not associated with cytotoxic drug administration. We report a patient treated with chemotherapy for recurrent lymphoma of the sigmoid colon, who developed a fistula between the large and small bowel which was associated with severe Endarteritis obliterans. A 66-year-old man presented to the surgeons with intermittent abdominal pain and diarrhoea. A barium enema demonstrated an annular narrowing in the sigmoid colon and the patient had a laparotomy which revealed a large tumour of the sigmoid colon invading the bladder and retroperitoneal structures.
C. J. M. Stephens - One of the best experts on this subject based on the ideXlab platform.
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Sneddon's syndrome.
Clinical and Experimental Rheumatology, 1992Co-Authors: C. J. M. StephensAbstract:: Sneddon's syndrome refers to the rare association of extensive livedo reticularis with multiple ischaemic cerebrovascular episodes. Endarteritis obliterans is the most common cutaneous pathology. It is likely that several pathogenic mechanisms may give rise to Sneddon's syndrome, as the condition is associated with a high incidence of generalised atherosclerosis, hypertension, valvular heart disease and the presence of antiphospholipid antibodies.
Rodrigo Morchón - One of the best experts on this subject based on the ideXlab platform.
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RESEARCH ARTICLE Fibrinolysis and Proliferative Endarteritis: Two Related Processes in Chronic Infections? The Model of the Blood-Borne Pathogen
2016Co-Authors: Dirofilaria Immitis, Javier González-miguel, Rodrigo Morchón, Mar Siles-lucas, O SimónAbstract:The interaction between blood-borne pathogens and fibrinolysis is one of the most impor-tant mechanisms that mediate invasion and the establishment of infectious agents in their hosts. However, overproduction of plasmin (final product of the route) has been related in other contexts to proliferation and migration of the arterial wall cells and degradation of the extracellular matrix. We have recently identified fibrinolysis-activating antigens from Dirofi-laria immitis, a blood-borne parasite whose key pathological event (proliferative Endarteritis) is produced by similar mechanisms to those indicated above. The objective of this work is to study how two of this antigens [actin (ACT) and fructose-bisphosphate aldolase (FBAL)] highly conserved in pathogens, activate fibrinolysis and to establish a relationship between this activation and the development of proliferative Endarteritis during cardiopulmonary diro-filariasis. We demonstrate that both proteins bind plasminogen, enhance plasmin genera-tion, stimulate the expression of the fibrinolytic activators tPA and uPA in endothelial cell cultures and are located on the surface of the worm in contact with the host’s blood. ELISA, western blot and immunofluorescence techniques were employed for this purpose. Addi
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glyceraldehyde 3 phosphate dehydrogenase and galectin from dirofilaria immitis participate in heartworm disease Endarteritis via plasminogen plasmin system
Veterinary Parasitology, 2016Co-Authors: Javier Gonzalezmiguel, Carmen Larrazabal, Diana Loameson, Mar Sileslucas, F Simon, Rodrigo MorchónAbstract:The interaction between parasitic protozoa and helminths, both in the blood and in tissues and the fibrinolytic system of their hosts is usually considered as a survival parasite mechanism since this system is the physiological route responsible for degrading fibrin clots. The broad-range proteolytic activity of plasmin, the final enzyme of the route, implies that its recruitment by these parasites is an important mechanism that mediates their invasion and establishment in the hosts. However, recent studies have proposed a dual role for plasmin by linking its over-production with pathological mechanisms at vascular level. Most of these studies have been conducted in Dirofilaria immitis, a blood-borne parasite that survives in the pulmonary arteries of its host for years while it produces a chronic inflammatory disease, whose main pathogenic mechanism is the appearance of proliferative Endarteritis. Recently, the participation of two proteins from D. immitis, glyceraldehyde 3-phosphate dehydrogenase (DiGAPDH) and galectin (DiGAL), in the activation of the fibrinolytic system of its host has been demonstrated, which has been a priori associated with parasite survival mechanisms. The aim of the present paper was to study the role of plasmin generated by these proteins in the emergence of proliferative Endarteritis. An in vitro model of canine endothelial and smooth muscle cells, as well as the two parasitic recombinant proteins were employed. The results show that DiGAPDH and DiGAL stimulate the proliferation and migration of both cell types, as well as the degradation of the extracellular matrix (ECM) via plasminogen (PLG)/plasmin system, being all of these mechanisms related to the appearance of proliferative Endarteritis. Due to the high degree of evolutionary conservation of these antigens, these data support the hypothesis of the survival/pathology ambivalence in the interactions between parasites and the fibrinolytic system of their hosts and represent an advance in the knowledge of the mechanisms involved in the emergence of proliferative Endarteritis in the cardiopulmonary dirofilariosis (heartworm disease).
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Glyceraldehyde 3-phosphate dehydrogenase and galectin from Dirofilaria immitis participate in heartworm disease Endarteritis via plasminogen/plasmin system.
Veterinary parasitology, 2016Co-Authors: Javier González-miguel, Fernando Simón, Carmen Larrazabal, Diana Loa-mesón, Mar Siles-lucas, Rodrigo MorchónAbstract:The interaction between parasitic protozoa and helminths, both in the blood and in tissues and the fibrinolytic system of their hosts is usually considered as a survival parasite mechanism since this system is the physiological route responsible for degrading fibrin clots. The broad-range proteolytic activity of plasmin, the final enzyme of the route, implies that its recruitment by these parasites is an important mechanism that mediates their invasion and establishment in the hosts. However, recent studies have proposed a dual role for plasmin by linking its over-production with pathological mechanisms at vascular level. Most of these studies have been conducted in Dirofilaria immitis, a blood-borne parasite that survives in the pulmonary arteries of its host for years while it produces a chronic inflammatory disease, whose main pathogenic mechanism is the appearance of proliferative Endarteritis. Recently, the participation of two proteins from D. immitis, glyceraldehyde 3-phosphate dehydrogenase (DiGAPDH) and galectin (DiGAL), in the activation of the fibrinolytic system of its host has been demonstrated, which has been a priori associated with parasite survival mechanisms. The aim of the present paper was to study the role of plasmin generated by these proteins in the emergence of proliferative Endarteritis. An in vitro model of canine endothelial and smooth muscle cells, as well as the two parasitic recombinant proteins were employed. The results show that DiGAPDH and DiGAL stimulate the proliferation and migration of both cell types, as well as the degradation of the extracellular matrix (ECM) via plasminogen (PLG)/plasmin system, being all of these mechanisms related to the appearance of proliferative Endarteritis. Due to the high degree of evolutionary conservation of these antigens, these data support the hypothesis of the survival/pathology ambivalence in the interactions between parasites and the fibrinolytic system of their hosts and represent an advance in the knowledge of the mechanisms involved in the emergence of proliferative Endarteritis in the cardiopulmonary dirofilariosis (heartworm disease).
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fibrinolysis and proliferative Endarteritis two related processes in chronic infections the model of the blood borne pathogen dirofilaria immitis
PLOS ONE, 2015Co-Authors: Javier Gonzalezmiguel, Rodrigo Morchón, Mar Sileslucas, F SimonAbstract:The interaction between blood-borne pathogens and fibrinolysis is one of the most important mechanisms that mediate invasion and the establishment of infectious agents in their hosts. However, overproduction of plasmin (final product of the route) has been related in other contexts to proliferation and migration of the arterial wall cells and degradation of the extracellular matrix. We have recently identified fibrinolysis-activating antigens from Dirofilaria immitis, a blood-borne parasite whose key pathological event (proliferative Endarteritis) is produced by similar mechanisms to those indicated above. The objective of this work is to study how two of this antigens [actin (ACT) and fructose-bisphosphate aldolase (FBAL)] highly conserved in pathogens, activate fibrinolysis and to establish a relationship between this activation and the development of proliferative Endarteritis during cardiopulmonary dirofilariasis. We demonstrate that both proteins bind plasminogen, enhance plasmin generation, stimulate the expression of the fibrinolytic activators tPA and uPA in endothelial cell cultures and are located on the surface of the worm in contact with the host’s blood. ELISA, western blot and immunofluorescence techniques were employed for this purpose. Additionally, the implication of lysine residues in this interaction was analyzed by bioinformatics. The involvement of plasmin generated by the ACT/FBAL and plasminogen binding in cell proliferation and migration, and degradation of the extracellular matrix were shown in an “in vitro” model of endothelial and smooth muscle cells in culture. The obtained results indicate that ACT and FBAL from D. immitis activate fibrinolysis, which could be used by the parasite like a survival mechanism to avoid the clot formation. However, long-term overproduction of plasmin can trigger pathological events similar to those described in the emergence of proliferative Endarteritis. Due to the high degree of evolutionary conservation of these antigens, similar processes may occur in other blood-borne pathogens.
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Can the activation of plasminogen/plasmin system of the host by metabolic products of Dirofilaria immitis participate in heartworm disease Endarteritis?
Parasites & vectors, 2015Co-Authors: Javier González-miguel, Rodrigo Morchón, Elena Carretón, José Alberto Montoya-alonso, Fernando SimónAbstract:Background Proliferative Endarteritis is one of the key pathological mechanisms of cardiopulmonary dirofilariosis, a cosmopolitan parasitosis caused by Dirofilaria immitis affecting dogs and cats around the world. It has been shown that the excretory/secretory antigens from D. immitis adult worms (DiES) bind plasminogen (PLG) and activate fibrinolysis, which can lead to a survival mechanism for the parasite in its intravascular environment. However, overproduction of plasmin (final product of the route) has been related to pathological processes similar to those described in proliferative Endarteritis. The aim of this study is to relate the appearance of this pathological condition with the activation of the PLG/plasmin system of the host by DiES.
Nmi Nana Miura Ikari - One of the best experts on this subject based on the ideXlab platform.
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p448infective endocarditis in patent ductus arteriosus by bartonella sp case report and literature review
Cardiovascular Research, 2014Co-Authors: Gbo Guilherme Benfatti Olivato, Frf Fernando Roberto De Fazzio, Tms Thaysa Moreira Santos, Mrp Milena Ribeiro Paixao, Wfg Wilton Francisco Gomes, Drgd Daniel Renato Goncalves Duarte, Tjgt Thais Juliano Garcia Tosta, Nmi Nana Miura IkariAbstract:Case report: APS, 19 years, female, outpatients with a diagnosis of patent ductus arteriosus in planning surgical correction sought emergency room presenting episodes of fever measured 2 weeks ago, asthenia and fatigue. Additional research showed pancytopenia and hepatosplenomegaly. Prompted transthoracic echocardiography showing orifice of the ductus arteriosus thicked image hiperrefringente, mobile, looks filamentous pedunculated measuring 13 mm in length, the topography of the ductus arteriosus, pulmonary attached to the end. Requested three pairs of blood cultures without detectable bacterial growth. Patient admitted for investigation and treatment of infective Endarteritis probable subacute seronegative blood channel, and also request surveys by indirect immunofluorescence for Bartonella sp, with positive results. Patient admitted for treatment with antibiotics and surgery scheduled for correction of patent ductus arteriosus, and started intravenous penicillin and gentamicin. During admission had septic symptomatic pulmonary embolization, followed by emergency surgery for correction of patent ductus arteriosus. Favorable outcome after high end drug treatment for 28 days. Discussion: Bartonella sp is a common cause of infectious endocarditis seronegative, with few reports in the literature. However, there are no reports in the literature National endarterites ductus arteriosus caused by such bacteria. Since this diagnosis is considered difficult at the beginning of the investigation, it should search request Bartonella sp in all cases of infective endocarditis and Endarteritis in patent ductus ateriosus with negative blood cultures, since the prevalence of this microorganism has been increasing in Brazil.