The Experts below are selected from a list of 147 Experts worldwide ranked by ideXlab platform

R.a Zvjagilskaya - One of the best experts on this subject based on the ideXlab platform.

Jozef Nosek - One of the best experts on this subject based on the ideXlab platform.

  • Development of a transformation system for the multinuclear yeast Dipodascus (Endomyces) magnusii.
    Yeast (Chichester England), 1998
    Co-Authors: L'ubica Adamíková, Peter Griač, Lubomir Tomaska, Jozef Nosek
    Abstract:

    We have developed the first system for genetic transformation of the multinuclear yeast Dipodascus magnusii. The system is based on a dominant selectable marker and an autonomously replicating sequence. We have constructed a plasmid vector which contains a marker conferring resistance to zeocin and the segment of non-transcribed spacer of D. magnusii ribosomal DNA which supports the autonomous replication of plasmid DNA in yeast cells. Plasmid DNA has been transferred into D. magnusii cells by electroporation. The DNA sequence which is described in this article has been deposited in the EMBL data library under Accession Number Y14587. © 1998 John Wiley & Sons, Ltd.

  • Electrophoretic karyotype of Dipodascus (Endomyces) magnusii: two main intraspecific chromosomal polymorphisms associated with the difference in total genome size
    Current Genetics, 1995
    Co-Authors: Dominik Filipp, Jozef Nosek, Peter Filipp, Mária Hladká
    Abstract:

    This study describes the karyotype of strain 270 of the yeast-like fungus Endomyces magnusii . It consists of 13 chromosomal DNA molecules, the size of which range between 1.2 and 5.7 Mb producing a genome size of approximately 38 Mb. By comparing the karyotype of six strains of E. magnusii , we revealed two main chromosome length polymorphisms (CLPs) associated with a pronounced difference in the total genome size (roughly 50%). Karyotype heterogeneity between two main CLPs was demonstrated by Southern analysis with three heterologous probes. The same species affiliation of six E. magnusii strains was confirmed by morphological and cytological studies, protein fingerprint comparisons, as well as restriction analysis of mitochondrial DNA and genomic Southern analysis.

  • Isolation of a dsRNA virus from Dipodascus (Endomyces) magnusii
    Current Genetics, 1993
    Co-Authors: Jozef Nosek, Dominik Filipp, Klára Bederkova, Peter Griač
    Abstract:

    Virus-like particles (VLPs) of 40 nm diameter were isolated from the yeast-like fungus Dipodascus magnusii . These VLPs copurify with several linear double-stranded RNA molecules of different size. We have found some polymorphism in both the length and the number of these dsRNAs among six D. magnusii strains. Analysis of CsCl gradient-purified VLPs on PAGE/SDS electrophoresis showed one major protein component with an apparent molecular weight of 75 kDa.

Jan Korb - One of the best experts on this subject based on the ideXlab platform.

  • Isolation and characterization of the dsRNA virus from the yeast Endomyces magnusii.
    FEMS microbiology letters, 1994
    Co-Authors: Martin Pospíšek, Zdena Palková, B. Janderová, Jan Korb
    Abstract:

    Virus-like particles (VLPs) have been isolated from the yeast Endomyces magnusii. The VLPs measure 43 nm in diameter and contain six species of dsRNA (0.78, 0.83, 1.77, 1.84, 2.64, 4.30 kb respectively). E. magnusii produces a ‘toxic’ protein, which reduces the growth, and changes the colony morphology, of sensitive strains of Hansenula sp. growing on solid media. All strains of E. magnusii tested produced the ‘toxin’ and contained the VLPs. Current procedures of curing failed to destroy the ability to produce the ‘toxin’.

Meredith Blackwell - One of the best experts on this subject based on the ideXlab platform.

  • four new yeasts in the candida mesenterica clade associated with basidiocarp feeding beetles
    Mycologia, 2005
    Co-Authors: Meredith Blackwell
    Abstract:

    We isolated five yeasts related to Candida mesenterica from the digestive tract, frass, and habitat of beetles in six families inhabiting basidiocarps. Based on rDNA sequence comparisons and pheno- typic characters, the yeasts were identified as Koda- maea ohmeri and four undescribed taxa. Phylogenetic analysis of combined small and large subunit ribo- somal DNA sequences placed the five taxa in a statis- tically well supported clade with C. mesenterica, Can- dida suecica and other yeast species known from basidiocarps, including 'Endomyces scopularum' (CBS154.92 and 155.92), Candida fukazawae, Can- dida fungicola and Candida sagamina. Only one of the new taxa produced ascospores; the other three reproduced only asexually. The yeasts appear to be less closely associated with beetles than with the bee- tle habitat. The new species and their type strains are Kodamaea laetipori (type strain NRRL Y-27713 T ), Candida derodonti (type strain NRRL Y-27711 T ), Can- dida arcana (type strain NRRL Y-27712 T ) and Can- dida plutei (type strain NRRL Y-27715 T ).

  • four new yeasts in the candida mesenterica clade associated with basidiocarp feeding beetles
    Mycologia, 2005
    Co-Authors: Meredith Blackwell
    Abstract:

    We isolated five yeasts related to Candida mesenterica from the digestive tract, frass, and habitat of beetles in six families inhabiting basidiocarps. Based on rDNA sequence comparisons and pheno- typic characters, the yeasts were identified as Koda- maea ohmeri and four undescribed taxa. Phylogenetic analysis of combined small and large subunit ribo- somal DNA sequences placed the five taxa in a statis- tically well supported clade with C. mesenterica, Can- dida suecica and other yeast species known from basidiocarps, including 'Endomyces scopularum' (CBS154.92 and 155.92), Candida fukazawae, Can- dida fungicola and Candida sagamina. Only one of the new taxa produced ascospores; the other three reproduced only asexually. The yeasts appear to be less closely associated with beetles than with the bee- tle habitat. The new species and their type strains are Kodamaea laetipori (type strain NRRL Y-27713 T ), Candida derodonti (type strain NRRL Y-27711 T ), Can- dida arcana (type strain NRRL Y-27712 T ) and Can- dida plutei (type strain NRRL Y-27715 T ).

  • The status of Endomyces scopularum-a filamentous fungus and two yeasts
    Mycologia, 2001
    Co-Authors: Sung-oui Suh, Cletus P. Kurtzman, Meredith Blackwell
    Abstract:

    Given the clouded history of species of En- domyces and the Endomycetales, we determined par- tial sequences of nuclear small and large subunits of the ribosomal RNA gene for three cultures of Endo- myces scopularum, a parasitic fungus of agaric basidi- ocarps, in order to clarify the phylogenetic position of the species within the ascomycetes. As the result of sequence comparisons, the three cultures were di- vided into two distinct groups in phylogenetic trees. Strain CBS 131.86 is in an ophiostomatalean clade in the euascomycetes. By comparison, CBS 154.92 and CBS 155.92, which have identical sequences in both subunits of rDNA, are in the Saccharomycetales clade. Candida fukazawae, C. sagamina, and C. fun- gicola, inhabitants of basidiocarps, were the closest taxa to the two yeast cultures.

O.a Bezborodova - One of the best experts on this subject based on the ideXlab platform.