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X Liu - One of the best experts on this subject based on the ideXlab platform.
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Genetic diagnosis and cochlear implantation for patients with nonsyndromic hearing loss and Enlarged Vestibular Aqueduct.
The Journal of Laryngology & Otology, 2012Co-Authors: R Lai, F Zhu, G Zhu, R Vivero, A Peng, Z Xiao, X Liu, D XieAbstract:Objective: To review the genotype and cochlear implantation outcome of patients with nonsyndromic hearing loss and Enlarged Vestibular Aqueduct. Methods: Twenty-one Chinese children with nonsyndromic hearing loss and Enlarged Vestibular Aqueduct underwent genetic examination. A DNA microarray was used to screen for the IVS7-2A>G and H723R mutations. Any DNA samples with one or none of the two mutant alleles were sequenced to detect other mutations in the SLC26A4 and FOXI1 genes. Results: Twelve SLC26A4 mutations were detected, including three novel mutations. The most common mutations detected were IVS7-2A>G and H723R. Twelve patients received cochlear implants, and subsequently demonstrated excellent speech perception. Conclusion: Three novel mutations were detected in Chinese patients with nonsyndromic hearing loss and Enlarged Vestibular Aqueduct. The SLC26A4 mutation spectrum in the Chinese population is similar to that in other East Asian populations. Cochlear implantation is a safe and effective treatment in patients with Enlarged Vestibular Aqueduct.
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original research otologymutation analysis of slc26a4 in mainland chinese patients with Enlarged Vestibular Aqueduct
Otolaryngology-Head and Neck Surgery, 2009Co-Authors: Samuel Reyes, Guojian Wang, Pu Dai, Xiaomei Ouyang, Bing Han, Hui Jun Yuan, Denise Yan, X LiuAbstract:Objective We have characterized the spectrum of SLC26A4 mutations and clinical features in a population of mainland Chinese patients with nonsyndromic sensorineural hearing loss (SNHL) and Enlarged Vestibular Aqueduct (EVA).
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mutation analysis of slc26a4 in mainland chinese patients with Enlarged Vestibular Aqueduct
Otolaryngology-Head and Neck Surgery, 2009Co-Authors: Samuel Reyes, Guojian Wang, Pu Dai, Xiaomei Ouyang, Bing Han, Hui Jun Yuan, Denise Yan, X LiuAbstract:OBJECTIVE: We have characterized the spectrum of SLC26A4 mutations and clinical features in a population of mainland Chinese patients with nonsyndromic sensorineural hearing loss (SNHL) and Enlarged Vestibular Aqueduct (EVA).STUDY DESIGN: Cross-sectional clinical genetic study.SETTING: Tertiary care outpatient otolaryngology clinic.METHODS: A total of 32 subjects identified with bilateral EVA using high-resolution CT were screened for mutations in SLC26A4 by denaturing high-performance liquid chromatography and direct sequencing methods.RESULTS: A total of 13 different mutations were identified in the SLC26A4 gene, five of which are novel. A total of 88 percent of the patients harbored biallelic mutations, 11 patients were homozygotes, and 17 were compound heterozygotes. Four patients were found to carry a single SLC26A4 mutation. The IVS7-2A>G mutation was the most frequent, accounting for 60 percent of the mutant alleles. We have not found any correlations between the type of SLC26A4 mutations and the t...
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Original research–otologyMutation analysis of SLC26A4 in mainland Chinese patients with Enlarged Vestibular Aqueduct
Otolaryngology–Head and Neck Surgery, 2009Co-Authors: Samuel Reyes, Guojian Wang, Pu Dai, Xiaomei Ouyang, Bing Han, Hui Jun Yuan, Denise Yan, X LiuAbstract:Objective We have characterized the spectrum of SLC26A4 mutations and clinical features in a population of mainland Chinese patients with nonsyndromic sensorineural hearing loss (SNHL) and Enlarged Vestibular Aqueduct (EVA).
Pu Dai - One of the best experts on this subject based on the ideXlab platform.
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SLC26A4 gene copy number variations in Chinese patients with non-syndromic Enlarged Vestibular Aqueduct.
Journal of Translational Medicine, 2012Co-Authors: Jiandong Zhao, Shasha Huang, Dongyi Han, Yongyi Yuan, Guojian Wang, Jing Chen, Pu DaiAbstract:Background Many patients with Enlarged Vestibular Aqueduct (EVA) have either only one allelic mutant of the SLC26A4 gene or lack any detectable mutation. In this study, multiplex ligation-dependent probe amplification (MLPA) was used to screen for copy number variations (CNVs) of SLC26A4 and to reveal the pathogenic mechanisms of non-syndromic EVA (NSEVA).
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Extremely discrepant mutation spectrum of SLC26A4 between Chinese patients with isolated Mondini deformity and Enlarged Vestibular Aqueduct
Journal of Translational Medicine, 2011Co-Authors: Shasha Huang, Dongyi Han, Yongyi Yuan, Guojian Wang, Dongyang Kang, Xin Zhang, Xiaofei Yan, Xiaoxiao Meng, Min Dong, Pu DaiAbstract:Background Mutations in SLC26A4 cause Pendred syndrome (hearing loss with goiter) or DFNB4 (non-syndromic hearing loss with inner ear malformation, such as Enlarged Vestibular Aqueduct or Mondini deformity). The relationship between mutations in SLC26A4 and Mondini deformity without Enlarged Vestibular Aqueduct has not been studied in any Chinese deaf population. The purpose of this study was to assess whether mutations in the SLC26A4 gene cause Mondini deformity without an Enlarged Vestibular Aqueduct (isolated Mondini deformity) in a Chinese population.
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Diagnostic function of SLC26A4 hot spot mutations screening to Enlarged Vestibular Aqueduct syndrome
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery, 2010Co-Authors: Ru-ping Fang, Yiwen You, Yong Wang, Pu DaiAbstract:OBJECTIVE To investigate the frequencies of SLC26A4 hot spot mutations by genetic testing method in non-syndromic hearing loss children. The feasibility of genetic screening method in finding Enlarged Vestibular Aqueduct syndrome was confirmed by temporal bone CT scan. METHOD Ninety-two children with moderate-profound hearing loss were enrolled and DNA were extracted from peripheral blood. SLC26A4 IVS7-2A > G and H723R mutations were analyzed by direct sequencing. The individual with homozygous, compound heterozygous or heterozygous SLC26A4 mutations was given further temporal CT scan. RESULT The sequencing results revealed 11 (12.0%) cases carrying SLC26A4 mutations, including 5 cases of bi-allelic mutation and 6 cases of single allelic mutation. CONCLUSION The SLC26A4 mutations has a high carrying rate in non-syndromic hearing loss children. The screening for the SLC26A4 gene mutations is useful in the diagnosis of EVAS.
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original research otologymutation analysis of slc26a4 in mainland chinese patients with Enlarged Vestibular Aqueduct
Otolaryngology-Head and Neck Surgery, 2009Co-Authors: Samuel Reyes, Guojian Wang, Pu Dai, Xiaomei Ouyang, Bing Han, Hui Jun Yuan, Denise Yan, X LiuAbstract:Objective We have characterized the spectrum of SLC26A4 mutations and clinical features in a population of mainland Chinese patients with nonsyndromic sensorineural hearing loss (SNHL) and Enlarged Vestibular Aqueduct (EVA).
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mutation analysis of slc26a4 in mainland chinese patients with Enlarged Vestibular Aqueduct
Otolaryngology-Head and Neck Surgery, 2009Co-Authors: Samuel Reyes, Guojian Wang, Pu Dai, Xiaomei Ouyang, Bing Han, Hui Jun Yuan, Denise Yan, X LiuAbstract:OBJECTIVE: We have characterized the spectrum of SLC26A4 mutations and clinical features in a population of mainland Chinese patients with nonsyndromic sensorineural hearing loss (SNHL) and Enlarged Vestibular Aqueduct (EVA).STUDY DESIGN: Cross-sectional clinical genetic study.SETTING: Tertiary care outpatient otolaryngology clinic.METHODS: A total of 32 subjects identified with bilateral EVA using high-resolution CT were screened for mutations in SLC26A4 by denaturing high-performance liquid chromatography and direct sequencing methods.RESULTS: A total of 13 different mutations were identified in the SLC26A4 gene, five of which are novel. A total of 88 percent of the patients harbored biallelic mutations, 11 patients were homozygotes, and 17 were compound heterozygotes. Four patients were found to carry a single SLC26A4 mutation. The IVS7-2A>G mutation was the most frequent, accounting for 60 percent of the mutant alleles. We have not found any correlations between the type of SLC26A4 mutations and the t...
Samuel Reyes - One of the best experts on this subject based on the ideXlab platform.
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mutation analysis of slc26a4 in mainland chinese patients with Enlarged Vestibular Aqueduct
Otolaryngology-Head and Neck Surgery, 2009Co-Authors: Samuel Reyes, Guojian Wang, Pu Dai, Xiaomei Ouyang, Bing Han, Hui Jun Yuan, Denise Yan, X LiuAbstract:OBJECTIVE: We have characterized the spectrum of SLC26A4 mutations and clinical features in a population of mainland Chinese patients with nonsyndromic sensorineural hearing loss (SNHL) and Enlarged Vestibular Aqueduct (EVA).STUDY DESIGN: Cross-sectional clinical genetic study.SETTING: Tertiary care outpatient otolaryngology clinic.METHODS: A total of 32 subjects identified with bilateral EVA using high-resolution CT were screened for mutations in SLC26A4 by denaturing high-performance liquid chromatography and direct sequencing methods.RESULTS: A total of 13 different mutations were identified in the SLC26A4 gene, five of which are novel. A total of 88 percent of the patients harbored biallelic mutations, 11 patients were homozygotes, and 17 were compound heterozygotes. Four patients were found to carry a single SLC26A4 mutation. The IVS7-2A>G mutation was the most frequent, accounting for 60 percent of the mutant alleles. We have not found any correlations between the type of SLC26A4 mutations and the t...
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original research otologymutation analysis of slc26a4 in mainland chinese patients with Enlarged Vestibular Aqueduct
Otolaryngology-Head and Neck Surgery, 2009Co-Authors: Samuel Reyes, Guojian Wang, Pu Dai, Xiaomei Ouyang, Bing Han, Hui Jun Yuan, Denise Yan, X LiuAbstract:Objective We have characterized the spectrum of SLC26A4 mutations and clinical features in a population of mainland Chinese patients with nonsyndromic sensorineural hearing loss (SNHL) and Enlarged Vestibular Aqueduct (EVA).
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Original research–otologyMutation analysis of SLC26A4 in mainland Chinese patients with Enlarged Vestibular Aqueduct
Otolaryngology–Head and Neck Surgery, 2009Co-Authors: Samuel Reyes, Guojian Wang, Pu Dai, Xiaomei Ouyang, Bing Han, Hui Jun Yuan, Denise Yan, X LiuAbstract:Objective We have characterized the spectrum of SLC26A4 mutations and clinical features in a population of mainland Chinese patients with nonsyndromic sensorineural hearing loss (SNHL) and Enlarged Vestibular Aqueduct (EVA).
Shin-ichi Usami - One of the best experts on this subject based on the ideXlab platform.
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Distribution and frequencies of PDS (SLC26A4) mutations in Pendred syndrome and nonsyndromic hearing loss associated with Enlarged Vestibular Aqueduct: a unique spectrum of mutations in Japanese
European Journal of Human Genetics, 2003Co-Authors: Koji Tsukamoto, Satoko Abe, Hiroaki Suzuki, Daisuke Harada, Atsushi Namba, Shin-ichi UsamiAbstract:Molecular diagnosis makes a substantial contribution to precise diagnosis, subclassification, prognosis, and selection of therapy. Mutations in the PDS (SLC26A4) gene are known to be responsible for both Pendred syndrome and nonsyndromic hearing loss associated with Enlarged Vestibular Aqueduct, and the molecular confirmation of the PDS gene has become important in the diagnosis of these conditions. In the present study, PDS mutation analysis confirmed that PDS mutations were present and significantly responsible in 90% of Pendred families, and in 78.1% of families with nonsyndromic hearing loss associated with Enlarged Vestibular Aqueduct. Furthermore, variable phenotypic expression by the same combination of mutations indicated that these two conditions are part of a continuous category of disease. Interestingly, the PDS mutation spectrum in Japanese, including the seven novel mutations revealed by this study, is very different from that found in Caucasians. Of the novel mutations detected, 53% were the H723R mutation, suggesting a possible founder effect. Ethnic background is therefore presumably important and should be noted when genetic testing is being performed. The PDS gene mutation spectrum in Japanese may be representative of those in Eastern Asian populations and its elucidation is expected to facilitate the molecular diagnosis of a variety of diseases.
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Non-syndromic hearing loss associated with Enlarged Vestibular Aqueduct is caused by PDS mutations
Human Genetics, 1999Co-Authors: Shin-ichi Usami, Satoko Abe, Michael D. Weston, Hideichi Shinkawa, Guy Van Camp, William J. KimberlingAbstract:Enlarged Vestibular Aqueduct (EVA), known as the most common form of inner ear abnormality, has recently been of particular genetic interest because this anomaly is inherited in a recessive manner. The locus for non-syndromic sensorineural hearing loss with EVA has been mapped to the same chromosomal region, 7q31, as the Pendred syndrome locus. In the present study, seven mutations in the PDS gene (PDS), the gene responsible for Pendred syndrome, have been found in families of non-syndromic sensorineural hearing loss with EVA. One family is homozygous, three families are compound heterozygotes, and two families are heterozygous but with no other mutation detected. The present results provide evidence that mutations in PDS cause both syndromic and non-syndromic hearing loss.
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fluctuating sensorineural hearing loss associated with Enlarged Vestibular Aqueduct maps to 7q31 the region containing the pendred gene
American Journal of Medical Genetics, 1999Co-Authors: Satoko Abe, Shin-ichi Usami, Hideichi Shinkawa, Denise M Hoover, Edward S Cohn, William J. KimberlingAbstract:The most common form of inner ear abnormality, Enlarged Vestibular Aqueduct (EVA), is of particular interest because it is associated with characteristic clinical findings, including fluctuating and sometimes progressive sensorineural hearing loss and disequilibrium symptoms. Although EVA has been reported to be inherited in a recessive manner, nothing else is known about the genetic basis of this hearing loss. Here we report on the localization of the gene responsible for sensorineural hearing loss associated with EVA to chromosomal region 7q31, with maximum multipoint LOD score of 3.647. The EVA candidate gene region lies in a 1.7-cM interval between the flanking markers D7S501 and D7S2425. Interestingly, this region overlaps the region containing the gene responsible for Pendred syndrome, called PDS, which was identified recently. However, the present subjects did not fulfill the criteria for Pendred syndrome. It is hypothesized that different mutations within the PDS gene may cause different phenotypes ranging from EVA to the Mondini deformity seen in Pendred syndrome.
Guojian Wang - One of the best experts on this subject based on the ideXlab platform.
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SLC26A4 gene copy number variations in Chinese patients with non-syndromic Enlarged Vestibular Aqueduct.
Journal of Translational Medicine, 2012Co-Authors: Jiandong Zhao, Shasha Huang, Dongyi Han, Yongyi Yuan, Guojian Wang, Jing Chen, Pu DaiAbstract:Background Many patients with Enlarged Vestibular Aqueduct (EVA) have either only one allelic mutant of the SLC26A4 gene or lack any detectable mutation. In this study, multiplex ligation-dependent probe amplification (MLPA) was used to screen for copy number variations (CNVs) of SLC26A4 and to reveal the pathogenic mechanisms of non-syndromic EVA (NSEVA).
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Extremely discrepant mutation spectrum of SLC26A4 between Chinese patients with isolated Mondini deformity and Enlarged Vestibular Aqueduct
Journal of Translational Medicine, 2011Co-Authors: Shasha Huang, Dongyi Han, Yongyi Yuan, Guojian Wang, Dongyang Kang, Xin Zhang, Xiaofei Yan, Xiaoxiao Meng, Min Dong, Pu DaiAbstract:Background Mutations in SLC26A4 cause Pendred syndrome (hearing loss with goiter) or DFNB4 (non-syndromic hearing loss with inner ear malformation, such as Enlarged Vestibular Aqueduct or Mondini deformity). The relationship between mutations in SLC26A4 and Mondini deformity without Enlarged Vestibular Aqueduct has not been studied in any Chinese deaf population. The purpose of this study was to assess whether mutations in the SLC26A4 gene cause Mondini deformity without an Enlarged Vestibular Aqueduct (isolated Mondini deformity) in a Chinese population.
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original research otologymutation analysis of slc26a4 in mainland chinese patients with Enlarged Vestibular Aqueduct
Otolaryngology-Head and Neck Surgery, 2009Co-Authors: Samuel Reyes, Guojian Wang, Pu Dai, Xiaomei Ouyang, Bing Han, Hui Jun Yuan, Denise Yan, X LiuAbstract:Objective We have characterized the spectrum of SLC26A4 mutations and clinical features in a population of mainland Chinese patients with nonsyndromic sensorineural hearing loss (SNHL) and Enlarged Vestibular Aqueduct (EVA).
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mutation analysis of slc26a4 in mainland chinese patients with Enlarged Vestibular Aqueduct
Otolaryngology-Head and Neck Surgery, 2009Co-Authors: Samuel Reyes, Guojian Wang, Pu Dai, Xiaomei Ouyang, Bing Han, Hui Jun Yuan, Denise Yan, X LiuAbstract:OBJECTIVE: We have characterized the spectrum of SLC26A4 mutations and clinical features in a population of mainland Chinese patients with nonsyndromic sensorineural hearing loss (SNHL) and Enlarged Vestibular Aqueduct (EVA).STUDY DESIGN: Cross-sectional clinical genetic study.SETTING: Tertiary care outpatient otolaryngology clinic.METHODS: A total of 32 subjects identified with bilateral EVA using high-resolution CT were screened for mutations in SLC26A4 by denaturing high-performance liquid chromatography and direct sequencing methods.RESULTS: A total of 13 different mutations were identified in the SLC26A4 gene, five of which are novel. A total of 88 percent of the patients harbored biallelic mutations, 11 patients were homozygotes, and 17 were compound heterozygotes. Four patients were found to carry a single SLC26A4 mutation. The IVS7-2A>G mutation was the most frequent, accounting for 60 percent of the mutant alleles. We have not found any correlations between the type of SLC26A4 mutations and the t...
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Original research–otologyMutation analysis of SLC26A4 in mainland Chinese patients with Enlarged Vestibular Aqueduct
Otolaryngology–Head and Neck Surgery, 2009Co-Authors: Samuel Reyes, Guojian Wang, Pu Dai, Xiaomei Ouyang, Bing Han, Hui Jun Yuan, Denise Yan, X LiuAbstract:Objective We have characterized the spectrum of SLC26A4 mutations and clinical features in a population of mainland Chinese patients with nonsyndromic sensorineural hearing loss (SNHL) and Enlarged Vestibular Aqueduct (EVA).