The Experts below are selected from a list of 42294 Experts worldwide ranked by ideXlab platform

Martina Mackova - One of the best experts on this subject based on the ideXlab platform.

  • whole cell maldi tof rapid screening method in Environmental Microbiology
    International Biodeterioration & Biodegradation, 2012
    Co-Authors: Jiri Koubek, Ondrej Uhlik, Katerina Jecna, Petra Junkova, Jana Vrkoslavova, Jan Lipov, Veronika Kurzawova, Tomas Macek, Martina Mackova
    Abstract:

    Abstract The rapidly developing field of Environmental Microbiology seeks reliable and fast methods for the identification of microorganisms. This work compared three methods for taxonomical identification of bacterial isolates obtained from sediment samples contaminated with polychlorinated biphenyls (PCBs). The methods compared were: biochemical set NEFERMtest 24, MALDI-TOF MS, and the sequencing of 16S rRNA genes. Use of NEFERMtest 24, which belongs to classical biochemical tests, showed a limited application. The MALDI-TOF method of mass spectrometry inferred existence of four different bacterial groups among the selected isolates. All the isolates were identified after sequencing of 16S rRNA genes in the Ribosomal Database Project. The sequencing method also proved the existence of four different taxonomical units among the isolates. This work showed a high potential of the mass spectrometry MALDI-TOF for screening and discrimination of different bacterial isolates with minimal time demand, reduced costs, and handling of the samples.

Jiri Koubek - One of the best experts on this subject based on the ideXlab platform.

  • whole cell maldi tof rapid screening method in Environmental Microbiology
    International Biodeterioration & Biodegradation, 2012
    Co-Authors: Jiri Koubek, Ondrej Uhlik, Katerina Jecna, Petra Junkova, Jana Vrkoslavova, Jan Lipov, Veronika Kurzawova, Tomas Macek, Martina Mackova
    Abstract:

    Abstract The rapidly developing field of Environmental Microbiology seeks reliable and fast methods for the identification of microorganisms. This work compared three methods for taxonomical identification of bacterial isolates obtained from sediment samples contaminated with polychlorinated biphenyls (PCBs). The methods compared were: biochemical set NEFERMtest 24, MALDI-TOF MS, and the sequencing of 16S rRNA genes. Use of NEFERMtest 24, which belongs to classical biochemical tests, showed a limited application. The MALDI-TOF method of mass spectrometry inferred existence of four different bacterial groups among the selected isolates. All the isolates were identified after sequencing of 16S rRNA genes in the Ribosomal Database Project. The sequencing method also proved the existence of four different taxonomical units among the isolates. This work showed a high potential of the mass spectrometry MALDI-TOF for screening and discrimination of different bacterial isolates with minimal time demand, reduced costs, and handling of the samples.

Veronika Kurzawova - One of the best experts on this subject based on the ideXlab platform.

  • whole cell maldi tof rapid screening method in Environmental Microbiology
    International Biodeterioration & Biodegradation, 2012
    Co-Authors: Jiri Koubek, Ondrej Uhlik, Katerina Jecna, Petra Junkova, Jana Vrkoslavova, Jan Lipov, Veronika Kurzawova, Tomas Macek, Martina Mackova
    Abstract:

    Abstract The rapidly developing field of Environmental Microbiology seeks reliable and fast methods for the identification of microorganisms. This work compared three methods for taxonomical identification of bacterial isolates obtained from sediment samples contaminated with polychlorinated biphenyls (PCBs). The methods compared were: biochemical set NEFERMtest 24, MALDI-TOF MS, and the sequencing of 16S rRNA genes. Use of NEFERMtest 24, which belongs to classical biochemical tests, showed a limited application. The MALDI-TOF method of mass spectrometry inferred existence of four different bacterial groups among the selected isolates. All the isolates were identified after sequencing of 16S rRNA genes in the Ribosomal Database Project. The sequencing method also proved the existence of four different taxonomical units among the isolates. This work showed a high potential of the mass spectrometry MALDI-TOF for screening and discrimination of different bacterial isolates with minimal time demand, reduced costs, and handling of the samples.

Tomas Macek - One of the best experts on this subject based on the ideXlab platform.

  • whole cell maldi tof rapid screening method in Environmental Microbiology
    International Biodeterioration & Biodegradation, 2012
    Co-Authors: Jiri Koubek, Ondrej Uhlik, Katerina Jecna, Petra Junkova, Jana Vrkoslavova, Jan Lipov, Veronika Kurzawova, Tomas Macek, Martina Mackova
    Abstract:

    Abstract The rapidly developing field of Environmental Microbiology seeks reliable and fast methods for the identification of microorganisms. This work compared three methods for taxonomical identification of bacterial isolates obtained from sediment samples contaminated with polychlorinated biphenyls (PCBs). The methods compared were: biochemical set NEFERMtest 24, MALDI-TOF MS, and the sequencing of 16S rRNA genes. Use of NEFERMtest 24, which belongs to classical biochemical tests, showed a limited application. The MALDI-TOF method of mass spectrometry inferred existence of four different bacterial groups among the selected isolates. All the isolates were identified after sequencing of 16S rRNA genes in the Ribosomal Database Project. The sequencing method also proved the existence of four different taxonomical units among the isolates. This work showed a high potential of the mass spectrometry MALDI-TOF for screening and discrimination of different bacterial isolates with minimal time demand, reduced costs, and handling of the samples.

Jana Vrkoslavova - One of the best experts on this subject based on the ideXlab platform.

  • whole cell maldi tof rapid screening method in Environmental Microbiology
    International Biodeterioration & Biodegradation, 2012
    Co-Authors: Jiri Koubek, Ondrej Uhlik, Katerina Jecna, Petra Junkova, Jana Vrkoslavova, Jan Lipov, Veronika Kurzawova, Tomas Macek, Martina Mackova
    Abstract:

    Abstract The rapidly developing field of Environmental Microbiology seeks reliable and fast methods for the identification of microorganisms. This work compared three methods for taxonomical identification of bacterial isolates obtained from sediment samples contaminated with polychlorinated biphenyls (PCBs). The methods compared were: biochemical set NEFERMtest 24, MALDI-TOF MS, and the sequencing of 16S rRNA genes. Use of NEFERMtest 24, which belongs to classical biochemical tests, showed a limited application. The MALDI-TOF method of mass spectrometry inferred existence of four different bacterial groups among the selected isolates. All the isolates were identified after sequencing of 16S rRNA genes in the Ribosomal Database Project. The sequencing method also proved the existence of four different taxonomical units among the isolates. This work showed a high potential of the mass spectrometry MALDI-TOF for screening and discrimination of different bacterial isolates with minimal time demand, reduced costs, and handling of the samples.