The Experts below are selected from a list of 3378 Experts worldwide ranked by ideXlab platform
Bruce E. Dale - One of the best experts on this subject based on the ideXlab platform.
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hemicellulases and auxiliary Enzymes for improved conversion of lignocellulosic biomass to monosaccharides
Biotechnology for Biofuels, 2011Co-Authors: Dahai Gao, Shishir P. S. Chundawat, Venkatesh Balan, Nirmal Uppugundla, Spencer Hermanson, Krishne Gowda, Phillip Brumm, David A Mead, Bruce E. DaleAbstract:Background High Enzyme loading is a major economic bottleneck for the commercial processing of pretreated lignocellulosic biomass to produce fermentable sugars. Optimizing the Enzyme Cocktail for specific types of pretreated biomass allows for a significant reduction in Enzyme loading without sacrificing hydrolysis yield. This is especially important for alkaline pretreatments such as Ammonia fiber expansion (AFEX) pretreated corn stover. Hence, a diverse set of hemicellulases supplemented along with cellulases is necessary for high recovery of monosaccharides.
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mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion afex pretreated corn stover
Bioresource Technology, 2010Co-Authors: Shishir P. S. Chundawat, Venkatesh Balan, Bruce E. Dale, Chandraraj KrishnanAbstract:In this work, six core glycosyl hydrolases (GH) were isolated and purified from various sources to help rationally optimize an Enzyme Cocktail to digest ammonia fiber expansion (AFEX) treated corn stover. The four core cellulases were endoglucanase I (EG I, GH family 7B), cellobiohydrolase I (CBH I, GH family 7A), cellobiohydrolase II (CBH II, GH family 6A) and β-glucosidase (βG, GH family 3). The two core hemicellulases were an endo-xylanase (EX, GH family 11) and a β-xylosidase (βX, GH family 3). Enzyme family and purity were confirmed by proteomics. Synergistic interactions among the six core Enzymes for varying relative and total protein loading (8.25, 16.5 and 33 mg/g glucan) during hydrolysis of AFEX-treated corn stover was studied using a high-throughput microplate based protocol. The optimal composition (based on% protein mass loading) of the Cocktail mixture was CBH I (28.4%): CBH II (18.0%): EG I (31.0%): EX (14.1%): βG (4.7%): βX (3.8%). These results demonstrate a rational strategy for the development of a minimal, synergistic Enzymes Cocktail that could reduce Enzyme usage and maximize the fermentable sugar yields from pretreated lignocellulosics.
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Mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion (AFEX) pretreated corn stover.
Bioresource technology, 2009Co-Authors: Dahai Gao, Shishir P. S. Chundawat, Venkatesh Balan, Chandraraj Krishnan, Bruce E. DaleAbstract:In this work, six core glycosyl hydrolases (GH) were isolated and purified from various sources to help rationally optimize an Enzyme Cocktail to digest ammonia fiber expansion (AFEX) treated corn stover. The four core cellulases were endoglucanase I (EG I, GH family 7B), cellobiohydrolase I (CBH I, GH family 7A), cellobiohydrolase II (CBH II, GH family 6A) and beta-glucosidase (betaG, GH family 3). The two core hemicellulases were an endo-xylanase (EX, GH family 11) and a beta-xylosidase (betaX, GH family 3). Enzyme family and purity were confirmed by proteomics. Synergistic interactions among the six core Enzymes for varying relative and total protein loading (8.25, 16.5 and 33 mg/g glucan) during hydrolysis of AFEX-treated corn stover was studied using a high-throughput microplate based protocol. The optimal composition (based on% protein mass loading) of the Cocktail mixture was CBH I (28.4%): CBH II (18.0%): EG I (31.0%): EX (14.1%): betaG (4.7%): betaX (3.8%). These results demonstrate a rational strategy for the development of a minimal, synergistic Enzymes Cocktail that could reduce Enzyme usage and maximize the fermentable sugar yields from pretreated lignocellulosics.
Jiao Jiao - One of the best experts on this subject based on the ideXlab platform.
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microwave assisted aqueous enzymatic extraction of oil from pumpkin seeds and evaluation of its physicochemical properties fatty acid compositions and antioxidant activities
Food Chemistry, 2014Co-Authors: Jiao Jiao, Zhugang Li, Xiaojuan Li, Yujie FuAbstract:Abstract Microwave-assisted aqueous enzymatic extraction (MAAEE) of pumpkin seed oil was performed in this study. An Enzyme Cocktail comprised of cellulase, pectinase and proteinase (w/w/w) was found to be the most effective in releasing oils. The highest oil recovery of 64.17% was achieved under optimal conditions of Enzyme concentration (1.4%, w/w), temperature (44 °C), time (66 min) and irradiation power (419 W). Moreover, there were no significant variations in physicochemical properties of MAAEE-extracted oil (MAAEEO) and Soxhlet-extracted oil (SEO), but MAAEEO exhibited better oxidation stability. Additionally, MAAEEO had a higher content of linoleic acid (57.33%) than SEO (53.72%), and it showed stronger antioxidant activities with the IC 50 values 123.93 and 152.84, mg/mL, according to DPPH radical scavenging assay and β-carotene/linoleic acid bleaching test. SEM results illustrated the destruction of cell walls and membranes by MAAEE. MAAEE is, therefore, a promising and environmental-friendly technique for oil extraction in the food industry.
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microwave assisted aqueous enzymatic extraction of oil from pumpkin seeds and evaluation of its physicochemical properties fatty acid compositions and antioxidant activities
Food Chemistry, 2014Co-Authors: Jiao Jiao, Qingyan Gai, Fuyao WeiAbstract:Abstract Microwave-assisted aqueous enzymatic extraction (MAAEE) of pumpkin seed oil was performed in this study. An Enzyme Cocktail comprised of cellulase, pectinase and proteinase (w/w/w) was found to be the most effective in releasing oils. The highest oil recovery of 64.17% was achieved under optimal conditions of Enzyme concentration (1.4%, w/w), temperature (44 °C), time (66 min) and irradiation power (419 W). Moreover, there were no significant variations in physicochemical properties of MAAEE-extracted oil (MAAEEO) and Soxhlet-extracted oil (SEO), but MAAEEO exhibited better oxidation stability. Additionally, MAAEEO had a higher content of linoleic acid (57.33%) than SEO (53.72%), and it showed stronger antioxidant activities with the IC 50 values 123.93 and 152.84, mg/mL, according to DPPH radical scavenging assay and β-carotene/linoleic acid bleaching test. SEM results illustrated the destruction of cell walls and membranes by MAAEE. MAAEE is, therefore, a promising and environmental-friendly technique for oil extraction in the food industry.
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Enzyme assisted aqueous extraction of oil from forsythia suspense seed and its physicochemical property and antioxidant activity
Industrial Crops and Products, 2013Co-Authors: Jiao Jiao, Yuangang Zu, Yujie FuAbstract:Abstract In order to exploit the byproduct of Forsythia suspense, its seed oil was first studied for the extraction, physicochemical property and antioxidant activity. The green and effective Enzyme-assisted aqueous extraction processing under microwave irradiation was applied to obtain the oil. The optimal oil yield of 21.62 ± 0.51% (dry weight of F. suspense seed basis) was achieved by the treatment of 1.5% (w/w) Enzyme Cocktail (cellulase/pectinase/proteinase = 1/1/1, w/w/w) under 90 min. The fatty acid composition of FSSO exhibited the predominance of linoleic acid (72.96%) along with oleic acid (18.66%) and palmitic acid (5.59%), which was quite similar to that of sunflower oil. Moreover, FSSO showed the notable concentration-dependent antioxidant activity with IC50 values of 22.35 and 29.51 mg/mL, according to the DPPH radical scavenging assay and β-carotene/linoleic acid bleaching test, respectively. Thus, FSSO may serve as a potential source of valuable product for the food, pharmaceutical, or cosmetics industries.
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microwave assisted aqueous enzymatic extraction of oil from isatis indigotica seeds and its evaluation of physicochemical properties fatty acid compositions and antioxidant activities
Industrial Crops and Products, 2013Co-Authors: Qingyan Gai, Jiao Jiao, Wei Wang, Meng Luo, Fuyao WeiAbstract:Abstract A novel and green microwave-assisted aqueous enzymatic extraction (MAAEE) of oil from Isatis indigotica seeds was investigated in the present study. Enzyme Cocktail (cellulase/proteinase/pectinase = 1/1/1, w/w/w) was found to be the most effective in releasing the oil. Under the optimal conditions as Enzyme concentration 1.82% (w/w), temperature 43 °C, time 83 min and irradiation power 375 W, the highest oil recovery of 59.27% was obtained using the emerging technique. As compared to conventional Soxhlet extraction-derived oil (SEO), MAAEE-derived oil (MAAEEO) revealed the similar physicochemical properties but exhibited better oxidation stability. In addition, MAAEEO had higher contents of valuable polyunsaturated fatty acids, tocopherols and phenolics as against SEO, and it exhibited considerable antioxidant activities with IC 50 values 9.58 and 15.03 mg/mL, according to DPPH radical scavenging assay and β -carotene/linoleic acid bleaching test, respectively. Scanning electron micrographs illustrated the severely structural disruptions of sample cell walls and membranes by MAAEE.
Fuyao Wei - One of the best experts on this subject based on the ideXlab platform.
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microwave assisted aqueous enzymatic extraction of oil from pumpkin seeds and evaluation of its physicochemical properties fatty acid compositions and antioxidant activities
Food Chemistry, 2014Co-Authors: Jiao Jiao, Qingyan Gai, Fuyao WeiAbstract:Abstract Microwave-assisted aqueous enzymatic extraction (MAAEE) of pumpkin seed oil was performed in this study. An Enzyme Cocktail comprised of cellulase, pectinase and proteinase (w/w/w) was found to be the most effective in releasing oils. The highest oil recovery of 64.17% was achieved under optimal conditions of Enzyme concentration (1.4%, w/w), temperature (44 °C), time (66 min) and irradiation power (419 W). Moreover, there were no significant variations in physicochemical properties of MAAEE-extracted oil (MAAEEO) and Soxhlet-extracted oil (SEO), but MAAEEO exhibited better oxidation stability. Additionally, MAAEEO had a higher content of linoleic acid (57.33%) than SEO (53.72%), and it showed stronger antioxidant activities with the IC 50 values 123.93 and 152.84, mg/mL, according to DPPH radical scavenging assay and β-carotene/linoleic acid bleaching test. SEM results illustrated the destruction of cell walls and membranes by MAAEE. MAAEE is, therefore, a promising and environmental-friendly technique for oil extraction in the food industry.
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microwave assisted aqueous enzymatic extraction of oil from isatis indigotica seeds and its evaluation of physicochemical properties fatty acid compositions and antioxidant activities
Industrial Crops and Products, 2013Co-Authors: Qingyan Gai, Jiao Jiao, Wei Wang, Meng Luo, Fuyao WeiAbstract:Abstract A novel and green microwave-assisted aqueous enzymatic extraction (MAAEE) of oil from Isatis indigotica seeds was investigated in the present study. Enzyme Cocktail (cellulase/proteinase/pectinase = 1/1/1, w/w/w) was found to be the most effective in releasing the oil. Under the optimal conditions as Enzyme concentration 1.82% (w/w), temperature 43 °C, time 83 min and irradiation power 375 W, the highest oil recovery of 59.27% was obtained using the emerging technique. As compared to conventional Soxhlet extraction-derived oil (SEO), MAAEE-derived oil (MAAEEO) revealed the similar physicochemical properties but exhibited better oxidation stability. In addition, MAAEEO had higher contents of valuable polyunsaturated fatty acids, tocopherols and phenolics as against SEO, and it exhibited considerable antioxidant activities with IC 50 values 9.58 and 15.03 mg/mL, according to DPPH radical scavenging assay and β -carotene/linoleic acid bleaching test, respectively. Scanning electron micrographs illustrated the severely structural disruptions of sample cell walls and membranes by MAAEE.
Yujie Fu - One of the best experts on this subject based on the ideXlab platform.
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microwave assisted aqueous enzymatic extraction of oil from pumpkin seeds and evaluation of its physicochemical properties fatty acid compositions and antioxidant activities
Food Chemistry, 2014Co-Authors: Jiao Jiao, Zhugang Li, Xiaojuan Li, Yujie FuAbstract:Abstract Microwave-assisted aqueous enzymatic extraction (MAAEE) of pumpkin seed oil was performed in this study. An Enzyme Cocktail comprised of cellulase, pectinase and proteinase (w/w/w) was found to be the most effective in releasing oils. The highest oil recovery of 64.17% was achieved under optimal conditions of Enzyme concentration (1.4%, w/w), temperature (44 °C), time (66 min) and irradiation power (419 W). Moreover, there were no significant variations in physicochemical properties of MAAEE-extracted oil (MAAEEO) and Soxhlet-extracted oil (SEO), but MAAEEO exhibited better oxidation stability. Additionally, MAAEEO had a higher content of linoleic acid (57.33%) than SEO (53.72%), and it showed stronger antioxidant activities with the IC 50 values 123.93 and 152.84, mg/mL, according to DPPH radical scavenging assay and β-carotene/linoleic acid bleaching test. SEM results illustrated the destruction of cell walls and membranes by MAAEE. MAAEE is, therefore, a promising and environmental-friendly technique for oil extraction in the food industry.
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Enzyme assisted aqueous extraction of oil from forsythia suspense seed and its physicochemical property and antioxidant activity
Industrial Crops and Products, 2013Co-Authors: Jiao Jiao, Yuangang Zu, Yujie FuAbstract:Abstract In order to exploit the byproduct of Forsythia suspense, its seed oil was first studied for the extraction, physicochemical property and antioxidant activity. The green and effective Enzyme-assisted aqueous extraction processing under microwave irradiation was applied to obtain the oil. The optimal oil yield of 21.62 ± 0.51% (dry weight of F. suspense seed basis) was achieved by the treatment of 1.5% (w/w) Enzyme Cocktail (cellulase/pectinase/proteinase = 1/1/1, w/w/w) under 90 min. The fatty acid composition of FSSO exhibited the predominance of linoleic acid (72.96%) along with oleic acid (18.66%) and palmitic acid (5.59%), which was quite similar to that of sunflower oil. Moreover, FSSO showed the notable concentration-dependent antioxidant activity with IC50 values of 22.35 and 29.51 mg/mL, according to the DPPH radical scavenging assay and β-carotene/linoleic acid bleaching test, respectively. Thus, FSSO may serve as a potential source of valuable product for the food, pharmaceutical, or cosmetics industries.
Venkatesh Balan - One of the best experts on this subject based on the ideXlab platform.
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novel thermostable endo xylanase cloned and expressed from bacterium geobacillus sp wsucf1
Bioresource Technology, 2014Co-Authors: Aditya Bhalla, Venkatesh Balan, Kenneth M Bischoff, Nirmal Uppugundla, Rajesh K SaniAbstract:A gene encoding a GH10 endo-xylanase from Geobacillus sp. WSUCF1 was cloned and expressed in Escherichia coli. Recombinant endo-xylanase (37kDa) exhibited high specific activity of 461.0U/mg of protein. Endo-xylanase was optimally active on birchwood xylan at 70°C and pH 6.5. The endo-xylanase was found to be highly thermostable at 50 and 60°C, retaining 82% and 50% of its original activity, respectively, after 60h. High xylan conversions (92%) were obtained with oat-spelt xylan hydrolysis. Higher glucan and xylan conversions were obtained on AFEX-treated corn stover with an Enzyme Cocktail containing WSUCF1 endo-xylanase (71% and 47%) as compared to Enzyme Cocktail containing commercial fungal endo-xylanase (64% and 41%). High specific activity, active at high pH's, wide substrate specificity, and higher hydrolytic activity on recalcitrant lignocellulose, make this endo-xylanase a suitable candidate for biofuel and bioprocess industries.
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hemicellulases and auxiliary Enzymes for improved conversion of lignocellulosic biomass to monosaccharides
Biotechnology for Biofuels, 2011Co-Authors: Dahai Gao, Shishir P. S. Chundawat, Venkatesh Balan, Nirmal Uppugundla, Spencer Hermanson, Krishne Gowda, Phillip Brumm, David A Mead, Bruce E. DaleAbstract:Background High Enzyme loading is a major economic bottleneck for the commercial processing of pretreated lignocellulosic biomass to produce fermentable sugars. Optimizing the Enzyme Cocktail for specific types of pretreated biomass allows for a significant reduction in Enzyme loading without sacrificing hydrolysis yield. This is especially important for alkaline pretreatments such as Ammonia fiber expansion (AFEX) pretreated corn stover. Hence, a diverse set of hemicellulases supplemented along with cellulases is necessary for high recovery of monosaccharides.
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mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion afex pretreated corn stover
Bioresource Technology, 2010Co-Authors: Shishir P. S. Chundawat, Venkatesh Balan, Bruce E. Dale, Chandraraj KrishnanAbstract:In this work, six core glycosyl hydrolases (GH) were isolated and purified from various sources to help rationally optimize an Enzyme Cocktail to digest ammonia fiber expansion (AFEX) treated corn stover. The four core cellulases were endoglucanase I (EG I, GH family 7B), cellobiohydrolase I (CBH I, GH family 7A), cellobiohydrolase II (CBH II, GH family 6A) and β-glucosidase (βG, GH family 3). The two core hemicellulases were an endo-xylanase (EX, GH family 11) and a β-xylosidase (βX, GH family 3). Enzyme family and purity were confirmed by proteomics. Synergistic interactions among the six core Enzymes for varying relative and total protein loading (8.25, 16.5 and 33 mg/g glucan) during hydrolysis of AFEX-treated corn stover was studied using a high-throughput microplate based protocol. The optimal composition (based on% protein mass loading) of the Cocktail mixture was CBH I (28.4%): CBH II (18.0%): EG I (31.0%): EX (14.1%): βG (4.7%): βX (3.8%). These results demonstrate a rational strategy for the development of a minimal, synergistic Enzymes Cocktail that could reduce Enzyme usage and maximize the fermentable sugar yields from pretreated lignocellulosics.
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Mixture optimization of six core glycosyl hydrolases for maximizing saccharification of ammonia fiber expansion (AFEX) pretreated corn stover.
Bioresource technology, 2009Co-Authors: Dahai Gao, Shishir P. S. Chundawat, Venkatesh Balan, Chandraraj Krishnan, Bruce E. DaleAbstract:In this work, six core glycosyl hydrolases (GH) were isolated and purified from various sources to help rationally optimize an Enzyme Cocktail to digest ammonia fiber expansion (AFEX) treated corn stover. The four core cellulases were endoglucanase I (EG I, GH family 7B), cellobiohydrolase I (CBH I, GH family 7A), cellobiohydrolase II (CBH II, GH family 6A) and beta-glucosidase (betaG, GH family 3). The two core hemicellulases were an endo-xylanase (EX, GH family 11) and a beta-xylosidase (betaX, GH family 3). Enzyme family and purity were confirmed by proteomics. Synergistic interactions among the six core Enzymes for varying relative and total protein loading (8.25, 16.5 and 33 mg/g glucan) during hydrolysis of AFEX-treated corn stover was studied using a high-throughput microplate based protocol. The optimal composition (based on% protein mass loading) of the Cocktail mixture was CBH I (28.4%): CBH II (18.0%): EG I (31.0%): EX (14.1%): betaG (4.7%): betaX (3.8%). These results demonstrate a rational strategy for the development of a minimal, synergistic Enzymes Cocktail that could reduce Enzyme usage and maximize the fermentable sugar yields from pretreated lignocellulosics.