The Experts below are selected from a list of 135852 Experts worldwide ranked by ideXlab platform
Corrado Girmenia - One of the best experts on this subject based on the ideXlab platform.
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Aspergillus galactomannan Enzyme-Linked Immunosorbent Assay cross-reactivity caused by invasive Geotrichum capitatum
2006Co-Authors: Mareva Giacchino, Nadia Chiapello, Stefania Bezzio, Paola Saracco, Vincenza Martini, Franca Fagioli, Giuseppe Cimino, Aida Alfarano, Pietro Martino, Corrado GirmeniaAbstract:We report three cases of invasive Geotrichum capitatum infection in patients with acute leukemia for which an Enzyme-Linked Immunosorbent Assay (ELISA) for Aspergillus galactomannan was positive, with no evidence of aspergillosis. Supernatants obtained from suspensions of 17 G. capitatum strains gave positive reactions with the Aspergillus galactomannan ELISA. These clinical and laboratory data seem to suggest that G. capitatum produces a soluble antigen that is cross-reactive with Aspergillus galactomannan.
G. Lo Cascio - One of the best experts on this subject based on the ideXlab platform.
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Cross-reactivity of Fusarium spp. in the Aspergillus galactomannan Enzyme-Linked Immunosorbent Assay
2012Co-Authors: Anna Maria Tortorano, Cristina Ossi, Maria Carmela Esposto, C Cavanna, Anna Prigitano, Anna Grancini, G. Lo CascioAbstract:Nine of 11 hematological patients with disseminated/deep-seated Fusarium infection tested at least twice for Aspergillus galactomannan (GM) had repeated positive results in the absence of Aspergillus isolation in culture. The centrifuged supernatants of 12 Fusarium isolates were tested by a GM Enzyme-Linked Immunosorbent Assay (EIA). All the isolates produced positive reactions when tested undiluted. These results show cross-reactivity of Fusarium spp. with Aspergillus GM that may constitute a drawback with respect to the specificity of the Platelia EIA.
Paul E Verweij - One of the best experts on this subject based on the ideXlab platform.
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Specificity of a sandwich Enzyme-Linked Immunosorbent Assay for detecting Aspergillus galactomannan
1997Co-Authors: C. M.a. Swanink, Jacques F.g.m. Meis, J. Peter Donnelly, Antonius J. M. M. Rijs, Paul E VerweijAbstract:The specificity of a sandwich Enzyme-Linked Immunosorbent Assay (ELISA) for detecting Aspergillus galactomannan was tested with exoantigens of 29 fungi cultured from clinical specimens. Cross-reactivity was observed with Penicillium chrysogenum, Penicillium digitatum, and Paecilomyces variotii. Furthermore, 40 serum samples obtained from bacteremic patients with hematologic malignancies were retrospectively tested by sandwich ELISA. False-positive reactions with the serum were reproducible but did not correspond with the results of culture of specific microorganisms. Moreover, the microorganisms cultured from the blood showed no reactivity by the sandwich ELISA.
Jean-paul Latgé - One of the best experts on this subject based on the ideXlab platform.
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A new sensitive sandwich Enzyme-Linked Immunosorbent Assay to detect galactofuran in patients with invasive Aspergillosis
1995Co-Authors: D Stynen, Annelies Goris, Jacqueline Sarfati, Jean-paul LatgéAbstract:A double-direct sandwich Enzyme-Linked Immunosorbent Assay that uses a rat anti-galactomannan monoclonal antibody as the acceptor and detector antibody was designed. This immunoAssay, which detects less than 1 ng of galactomannan per ml, was assessed in a retrospective study with samples from patients with invasive aspergillosis. Serum is more appropriate than urine for use in the search for circulating galactomannan. Antigenemia does not have a transient character. Galactomannan can be detected at least 39 days before the death of the patients.
Mareva Giacchino - One of the best experts on this subject based on the ideXlab platform.
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Aspergillus galactomannan Enzyme-Linked Immunosorbent Assay cross-reactivity caused by invasive Geotrichum capitatum
2006Co-Authors: Mareva Giacchino, Nadia Chiapello, Stefania Bezzio, Paola Saracco, Vincenza Martini, Franca Fagioli, Giuseppe Cimino, Aida Alfarano, Pietro Martino, Corrado GirmeniaAbstract:We report three cases of invasive Geotrichum capitatum infection in patients with acute leukemia for which an Enzyme-Linked Immunosorbent Assay (ELISA) for Aspergillus galactomannan was positive, with no evidence of aspergillosis. Supernatants obtained from suspensions of 17 G. capitatum strains gave positive reactions with the Aspergillus galactomannan ELISA. These clinical and laboratory data seem to suggest that G. capitatum produces a soluble antigen that is cross-reactive with Aspergillus galactomannan.