The Experts below are selected from a list of 12489 Experts worldwide ranked by ideXlab platform
Pawan Kumar Maurya - One of the best experts on this subject based on the ideXlab platform.
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Epigallocatechin-3-Gallate Protects Erythrocyte Ca(2+)-ATPase and Na(+)/K(+)-ATPase Against Oxidative Induced Damage During Aging in Humans.
Advanced Pharmaceutical Bulletin, 2014Co-Authors: Prabhanshu Kumar, Pawan Kumar MauryaAbstract:Purpose: The main purpose of this study was to investigate the protective role of epigallocatechin-3-Gallate on tertiary butyl hydroperoxide induced oxidative damage in erythrocyte during aging in humans. Methods: Human erythrocyte membrane bound Ca2+-ATPase and Na+/K+-ATPase activities were determined as a function of human age. Protective role of epigallocatechin-3-Gallate was evaluated by in vitro experiments by adding epigallocatechin-3-Gallate in concentration dependent manner (final concentration range 10-7M to 10-4M) to the enzyme assay medium. Oxidative stress was induced in vitro by incubating washed erythrocyte ghosts with tertiary butyl hydroperoxide (10-5 M final concentration). Results: We have reported concentration dependent effect of epigallocatechin-3-Gallate on tertiary butyl hydroperoxide induced damage on activities of Ca2+-ATPase and Na+/K+-ATPase during aging in humans. We have detected a significant (p < 0.001) decreased activity of Ca2+-ATPase and Na+/K+ -ATPase as a function of human age. Epigallocatechin-3-Gallate protected ATPases against tertiary butyl hydroperoxide induced damage in concentration dependent manner during aging in humans. Conclusion: Epigallocatechin-3-Gallate is a powerful antioxidant that is capable of protecting erythrocyte Ca2+-ATPase and Na+/K+ -ATPase against oxidative stress during aging in humans. We may propose hypothesis that a high intake of catechin rich diet may provide some protection against development of aging and age related diseases.
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Epigallocatechin-3-Gallate Protects Erythrocyte Ca(2+)-ATPase and Na(+)/K(+)-ATPase Against Oxidative Induced Damage During Aging in Humans.
Advanced pharmaceutical bulletin, 2014Co-Authors: Prabhanshu Kumar, Pawan Kumar MauryaAbstract:The main purpose of this study was to investigate the protective role of epigallocatechin-3-Gallate on tertiary butyl hydroperoxide induced oxidative damage in erythrocyte during aging in humans. Human erythrocyte membrane bound Ca(2+)-ATPase and Na(+)/K(+)-ATPase activities were determined as a function of human age. Protective role of epigallocatechin-3-Gallate was evaluated by in vitro experiments by adding epigallocatechin-3-Gallate in concentration dependent manner (final concentration range 10(-7)M to 10(-4)M) to the enzyme assay medium. Oxidative stress was induced in vitro by incubating washed erythrocyte ghosts with tertiary butyl hydroperoxide (10(-5) M final concentration). We have reported concentration dependent effect of epigallocatechin-3-Gallate on tertiary butyl hydroperoxide induced damage on activities of Ca(2+)-ATPase and Na(+)/K(+)-ATPase during aging in humans. We have detected a significant (p < 0.001) decreased activity of Ca(2+)-ATPase and Na(+)/K(+) -ATPase as a function of human age. Epigallocatechin-3-Gallate protected ATPases against tertiary butyl hydroperoxide induced damage in concentration dependent manner during aging in humans. Epigallocatechin-3-Gallate is a powerful antioxidant that is capable of protecting erythrocyte Ca(2+)-ATPase and Na(+)/K(+) -ATPase against oxidative stress during aging in humans. We may propose hypothesis that a high intake of catechin rich diet may provide some protection against development of aging and age related diseases.
Hasan Mukhtar - One of the best experts on this subject based on the ideXlab platform.
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inhibition of ultraviolet b mediated activation of nuclear factor κb in normal human epidermal keratinocytes by green tea constituent epigallocatechin 3 gallate
Oncogene, 2003Co-Authors: Farrukh Afaq, Vaqar M Adhami, Nihal Ahmad, Hasan MukhtarAbstract:Inhibition of ultraviolet B-mediated activation of nuclear factor κ B in normal human epidermal keratinocytes by green tea Constituent (-)-epigallocatechin-3-Gallate
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Inhibition of ultraviolet B-mediated activation of nuclear factor κB in normal human epidermal keratinocytes by green tea Constituent (-)-epigallocatechin-3-Gallate
Oncogene, 2003Co-Authors: Farrukh Afaq, Vaqar M Adhami, Nihal Ahmad, Hasan MukhtarAbstract:Inhibition of ultraviolet B-mediated activation of nuclear factor κ B in normal human epidermal keratinocytes by green tea Constituent (-)-epigallocatechin-3-Gallate
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green tea constituent epigallocatechin 3 gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells
Journal of the National Cancer Institute, 1997Co-Authors: Nihal Ahmad, Denise K Feyes, Hasan Mukhtar, Rajesh Agarwal, Anna Liisa NieminenAbstract:Background and Purpose: The polyphenolic compounds present in green tea show cancer chemopreventive effects in many animal tumor models. Epidemiologic studies have also suggested that green tea consumption might be effective in the prevention of certain human cancers. We investigated the effect of green tea polyphenols and the major constituent, epigallocatechin-3-Gallate, on the induction of apoptosis (programmed cell death) and regulation of cell cycle in human and mouse carcinoma cells. Methods: Human epidermoid carcinoma cells (cell line A431), human carcinoma keratinocyte (cell line HaCaT), human prostate carcinoma cells (cell line DU145), mouse lymphoma cells (cell line L5178Y), and normal human epidermal keratinocytes (NHEKs) were used. Apoptosis was assessed by 1) the formation of internucleosomal DNA fragments by agarose gel electrophoresis, 2) confocal microscopy, and 3) flow cytometry after tagging the DNA fragments by fluorescence label. The distribution of cells in different phases of the cell cycle was analyzed by flow cytometry. Results: Treatment of A431 cells with green tea polyphenols and its components, epigallocatechin-3-Gallate, epigallocatechin, and epicatechin-3-Gallate, resulted in the formation of internucleo-somal DNA fragments, characteristic of apoptosis. Treatment with epigallocatechin-3-Gallate also resulted in apoptosis in HaCaT, L5178Y, and DU145 cells, but not in NHEK. Confocal microscopy and flow cytometry confirmed the findings. The DNA cell cycle analysis showed that in A431 cells, epigallocatechin-3-Gallate treatment resulted in arrest in the G 0 -G 1 phase of the cell cycle and a dose-dependent apoptosis. Conclusions: Green tea may protect against cancer by causing cell cycle arrest and inducing apoptosis. It needs to be evaluated in human trials.
Prabhanshu Kumar - One of the best experts on this subject based on the ideXlab platform.
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Epigallocatechin-3-Gallate Protects Erythrocyte Ca(2+)-ATPase and Na(+)/K(+)-ATPase Against Oxidative Induced Damage During Aging in Humans.
Advanced Pharmaceutical Bulletin, 2014Co-Authors: Prabhanshu Kumar, Pawan Kumar MauryaAbstract:Purpose: The main purpose of this study was to investigate the protective role of epigallocatechin-3-Gallate on tertiary butyl hydroperoxide induced oxidative damage in erythrocyte during aging in humans. Methods: Human erythrocyte membrane bound Ca2+-ATPase and Na+/K+-ATPase activities were determined as a function of human age. Protective role of epigallocatechin-3-Gallate was evaluated by in vitro experiments by adding epigallocatechin-3-Gallate in concentration dependent manner (final concentration range 10-7M to 10-4M) to the enzyme assay medium. Oxidative stress was induced in vitro by incubating washed erythrocyte ghosts with tertiary butyl hydroperoxide (10-5 M final concentration). Results: We have reported concentration dependent effect of epigallocatechin-3-Gallate on tertiary butyl hydroperoxide induced damage on activities of Ca2+-ATPase and Na+/K+-ATPase during aging in humans. We have detected a significant (p < 0.001) decreased activity of Ca2+-ATPase and Na+/K+ -ATPase as a function of human age. Epigallocatechin-3-Gallate protected ATPases against tertiary butyl hydroperoxide induced damage in concentration dependent manner during aging in humans. Conclusion: Epigallocatechin-3-Gallate is a powerful antioxidant that is capable of protecting erythrocyte Ca2+-ATPase and Na+/K+ -ATPase against oxidative stress during aging in humans. We may propose hypothesis that a high intake of catechin rich diet may provide some protection against development of aging and age related diseases.
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Epigallocatechin-3-Gallate Protects Erythrocyte Ca(2+)-ATPase and Na(+)/K(+)-ATPase Against Oxidative Induced Damage During Aging in Humans.
Advanced pharmaceutical bulletin, 2014Co-Authors: Prabhanshu Kumar, Pawan Kumar MauryaAbstract:The main purpose of this study was to investigate the protective role of epigallocatechin-3-Gallate on tertiary butyl hydroperoxide induced oxidative damage in erythrocyte during aging in humans. Human erythrocyte membrane bound Ca(2+)-ATPase and Na(+)/K(+)-ATPase activities were determined as a function of human age. Protective role of epigallocatechin-3-Gallate was evaluated by in vitro experiments by adding epigallocatechin-3-Gallate in concentration dependent manner (final concentration range 10(-7)M to 10(-4)M) to the enzyme assay medium. Oxidative stress was induced in vitro by incubating washed erythrocyte ghosts with tertiary butyl hydroperoxide (10(-5) M final concentration). We have reported concentration dependent effect of epigallocatechin-3-Gallate on tertiary butyl hydroperoxide induced damage on activities of Ca(2+)-ATPase and Na(+)/K(+)-ATPase during aging in humans. We have detected a significant (p < 0.001) decreased activity of Ca(2+)-ATPase and Na(+)/K(+) -ATPase as a function of human age. Epigallocatechin-3-Gallate protected ATPases against tertiary butyl hydroperoxide induced damage in concentration dependent manner during aging in humans. Epigallocatechin-3-Gallate is a powerful antioxidant that is capable of protecting erythrocyte Ca(2+)-ATPase and Na(+)/K(+) -ATPase against oxidative stress during aging in humans. We may propose hypothesis that a high intake of catechin rich diet may provide some protection against development of aging and age related diseases.
Nihal Ahmad - One of the best experts on this subject based on the ideXlab platform.
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inhibition of ultraviolet b mediated activation of nuclear factor κb in normal human epidermal keratinocytes by green tea constituent epigallocatechin 3 gallate
Oncogene, 2003Co-Authors: Farrukh Afaq, Vaqar M Adhami, Nihal Ahmad, Hasan MukhtarAbstract:Inhibition of ultraviolet B-mediated activation of nuclear factor κ B in normal human epidermal keratinocytes by green tea Constituent (-)-epigallocatechin-3-Gallate
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Inhibition of ultraviolet B-mediated activation of nuclear factor κB in normal human epidermal keratinocytes by green tea Constituent (-)-epigallocatechin-3-Gallate
Oncogene, 2003Co-Authors: Farrukh Afaq, Vaqar M Adhami, Nihal Ahmad, Hasan MukhtarAbstract:Inhibition of ultraviolet B-mediated activation of nuclear factor κ B in normal human epidermal keratinocytes by green tea Constituent (-)-epigallocatechin-3-Gallate
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green tea constituent epigallocatechin 3 gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells
Journal of the National Cancer Institute, 1997Co-Authors: Nihal Ahmad, Denise K Feyes, Hasan Mukhtar, Rajesh Agarwal, Anna Liisa NieminenAbstract:Background and Purpose: The polyphenolic compounds present in green tea show cancer chemopreventive effects in many animal tumor models. Epidemiologic studies have also suggested that green tea consumption might be effective in the prevention of certain human cancers. We investigated the effect of green tea polyphenols and the major constituent, epigallocatechin-3-Gallate, on the induction of apoptosis (programmed cell death) and regulation of cell cycle in human and mouse carcinoma cells. Methods: Human epidermoid carcinoma cells (cell line A431), human carcinoma keratinocyte (cell line HaCaT), human prostate carcinoma cells (cell line DU145), mouse lymphoma cells (cell line L5178Y), and normal human epidermal keratinocytes (NHEKs) were used. Apoptosis was assessed by 1) the formation of internucleosomal DNA fragments by agarose gel electrophoresis, 2) confocal microscopy, and 3) flow cytometry after tagging the DNA fragments by fluorescence label. The distribution of cells in different phases of the cell cycle was analyzed by flow cytometry. Results: Treatment of A431 cells with green tea polyphenols and its components, epigallocatechin-3-Gallate, epigallocatechin, and epicatechin-3-Gallate, resulted in the formation of internucleo-somal DNA fragments, characteristic of apoptosis. Treatment with epigallocatechin-3-Gallate also resulted in apoptosis in HaCaT, L5178Y, and DU145 cells, but not in NHEK. Confocal microscopy and flow cytometry confirmed the findings. The DNA cell cycle analysis showed that in A431 cells, epigallocatechin-3-Gallate treatment resulted in arrest in the G 0 -G 1 phase of the cell cycle and a dose-dependent apoptosis. Conclusions: Green tea may protect against cancer by causing cell cycle arrest and inducing apoptosis. It needs to be evaluated in human trials.
Maria Luisa García - One of the best experts on this subject based on the ideXlab platform.
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Epigallocatechin-3-Gallate loaded PEGylated-PLGA nanoparticles: A new anti-seizure strategy for temporal lobe epilepsy
Nanomedicine : nanotechnology biology and medicine, 2018Co-Authors: Amanda Cano, Miren Ettcheto, Marta Espina, C. Auladell, Ana C. Calpena, Jaume Folch, Marta Barenys, Elena Sánchez-lópez, Antoni Camins, Maria Luisa GarcíaAbstract:Abstract Temporal lobe epilepsy is the most common type of pharmacoresistant epilepsy in adults. Epigallocatechin-3-Gallate has aroused much interest because of its multiple therapeutic effects, but its instability compromises the potential effectiveness. PEGylated-PLGA nanoparticles of Epigallocatechin-3-Gallate were designed to protect the drug and to increase the brain delivery. Nanoparticles were prepared by the double emulsion method and cytotoxicity, behavioral, Fluoro-Jade C, Iba1 and GFAP immunohistochemistry studies were carried out to determine their effectiveness. Nanoparticles showed an average size of 169 nm, monodisperse population, negative surface charge, encapsulation efficiency of 95% and sustained release profile. Cytotoxicity assays exhibited that these nanocarriers were non-toxic. Behavioral test showed that nanoparticles reduced most than free drug the number of epileptic episodes and their intensity. Neurotoxicity and immunohistochemistry studies confirmed a decrease in neuronal death and neuroinflammation. In conclusion, Epigallocatechin-3-Gallate PEGylated-PLGA nanoparticles could be a suitable strategy for the treatment of temporal lobe epilepsy.
