The Experts below are selected from a list of 288 Experts worldwide ranked by ideXlab platform

Sándor Pongor - One of the best experts on this subject based on the ideXlab platform.

  • Distribution of sequence‐dependent curvature in genomic DNA sequences
    FEBS Letters, 1997
    Co-Authors: Andrei Gabrielian, Kristian Vlahoviček, Sándor Pongor
    Abstract:

    The distribution of inherent, sequence-dependent curvature was calculated for a number of prokaryotic (M. genitalium, H. influenzae, M. jannaschii), viral (adenovirus 2, Equine Herpes virus 1), phage (M13, lambda), eukaryotic (S. cerevisiae) and mitochondrial genomes as well as E. coli and human genomic fragments. The genomic averages are in the range of 6–8°/helical turn and only about 20% of DNA is curved less than 3°/helical turn. The prokaryotes and phages appear to have a consistently higher frequency of curved DNA in their genomes than the other genomes tested. Long, highly curved segments, similar to artificially designed curved DNA, are apparently absent from the genomes. Short, curved segments, differing in G+C content may provide environmentally modulated conformational signals for gene regulation. A WWW-server was constructed for the prediction of curved sites from DNA sequences 〈http://icgeb.trieste.it/dna/curve_it.html/〉.

  • Distribution of sequence-dependent curvature in genomic DNA sequences.
    FEBS letters, 1997
    Co-Authors: Andrei Gabrielian, Kristian Vlahoviček, Sándor Pongor
    Abstract:

    The distribution of inherent, sequence-dependent curvature was calculated for a number of prokaryotic (M. genitalium, H. influenzae, M. jannaschii), viral (adenovirus 2, Equine Herpes virus 1), phage (M13, lambda), eukaryotic (S. cerevisiae) and mitochondrial genomes as well as E. coli and human genomic fragments. The genomic averages are in the range of 6-8 degrees/helical turn and only about 20% of DNA is curved less than 3 degrees/helical turn. The prokaryotes and phages appear to have a consistently higher frequency of curved DNA in their genomes than the other genomes tested. Long, highly curved segments, similar to artificially designed curved DNA, are apparently absent from the genomes. Short, curved segments, differing in G+C content may provide environmentally modulated conformational signals for gene regulation. A WWW-server was constructed for the prediction of curved sites from DNA sequences (http://icgeb.trieste.it/dna/curve_it.html/)..

D Klatzmann - One of the best experts on this subject based on the ideXlab platform.

  • The Equine Herpes virus 4 thymidine kinase is a better suicide gene than the human Herpes virus 1 thymidine kinase
    Gene Therapy, 1999
    Co-Authors: L Loubière, M Tiraby, C Cazaux, E Brisson, M Grisoni, J C Zhao-emonet, G Tiraby, D Klatzmann
    Abstract:

    The Herpes simplex virus type 1 thymidine kinase suicide gene (HSV1tk) together with ganciclovir (GCV) have been successfully used for in vivo treatment of various experimental tumors, and many clinical trials using this system have been launched. With the aim to improve this therapeutic system, we compared the potential efficacy of different Herpes virus derived thymidine kinases (HSV1, varicella-zoster virus, Equine Herpes virus type-4 and Epstein–Barr virus) as suicide genes in association with the nucleoside analogs acyclovir, ganciclovir and bromovinyldeoxyur- idine. Using various murine and human cell lines expressing these viral tk, we show that HSV1- and EHV4tk are the more efficient suicide genes for the different nucleoside analogs tested. Moreover, EHV4tk expressing murine and human cells were three- to 12-fold more sensitive to GCV than HSV1tk expressing cells. This was correlated with the presence of five-fold higher amounts of the toxic triphosphated-GCV in EHV4- versus HSV1tk expressing cells. Altogether, these experiments underline the potential advantages of the EHV4tk as a suicide gene.

Andrei Gabrielian - One of the best experts on this subject based on the ideXlab platform.

  • Distribution of sequence‐dependent curvature in genomic DNA sequences
    FEBS Letters, 1997
    Co-Authors: Andrei Gabrielian, Kristian Vlahoviček, Sándor Pongor
    Abstract:

    The distribution of inherent, sequence-dependent curvature was calculated for a number of prokaryotic (M. genitalium, H. influenzae, M. jannaschii), viral (adenovirus 2, Equine Herpes virus 1), phage (M13, lambda), eukaryotic (S. cerevisiae) and mitochondrial genomes as well as E. coli and human genomic fragments. The genomic averages are in the range of 6–8°/helical turn and only about 20% of DNA is curved less than 3°/helical turn. The prokaryotes and phages appear to have a consistently higher frequency of curved DNA in their genomes than the other genomes tested. Long, highly curved segments, similar to artificially designed curved DNA, are apparently absent from the genomes. Short, curved segments, differing in G+C content may provide environmentally modulated conformational signals for gene regulation. A WWW-server was constructed for the prediction of curved sites from DNA sequences 〈http://icgeb.trieste.it/dna/curve_it.html/〉.

  • Distribution of sequence-dependent curvature in genomic DNA sequences.
    FEBS letters, 1997
    Co-Authors: Andrei Gabrielian, Kristian Vlahoviček, Sándor Pongor
    Abstract:

    The distribution of inherent, sequence-dependent curvature was calculated for a number of prokaryotic (M. genitalium, H. influenzae, M. jannaschii), viral (adenovirus 2, Equine Herpes virus 1), phage (M13, lambda), eukaryotic (S. cerevisiae) and mitochondrial genomes as well as E. coli and human genomic fragments. The genomic averages are in the range of 6-8 degrees/helical turn and only about 20% of DNA is curved less than 3 degrees/helical turn. The prokaryotes and phages appear to have a consistently higher frequency of curved DNA in their genomes than the other genomes tested. Long, highly curved segments, similar to artificially designed curved DNA, are apparently absent from the genomes. Short, curved segments, differing in G+C content may provide environmentally modulated conformational signals for gene regulation. A WWW-server was constructed for the prediction of curved sites from DNA sequences (http://icgeb.trieste.it/dna/curve_it.html/)..

L Loubière - One of the best experts on this subject based on the ideXlab platform.

  • The Equine Herpes virus 4 thymidine kinase is a better suicide gene than the human Herpes virus 1 thymidine kinase
    Gene Therapy, 1999
    Co-Authors: L Loubière, M Tiraby, C Cazaux, E Brisson, M Grisoni, J C Zhao-emonet, G Tiraby, D Klatzmann
    Abstract:

    The Herpes simplex virus type 1 thymidine kinase suicide gene (HSV1tk) together with ganciclovir (GCV) have been successfully used for in vivo treatment of various experimental tumors, and many clinical trials using this system have been launched. With the aim to improve this therapeutic system, we compared the potential efficacy of different Herpes virus derived thymidine kinases (HSV1, varicella-zoster virus, Equine Herpes virus type-4 and Epstein–Barr virus) as suicide genes in association with the nucleoside analogs acyclovir, ganciclovir and bromovinyldeoxyur- idine. Using various murine and human cell lines expressing these viral tk, we show that HSV1- and EHV4tk are the more efficient suicide genes for the different nucleoside analogs tested. Moreover, EHV4tk expressing murine and human cells were three- to 12-fold more sensitive to GCV than HSV1tk expressing cells. This was correlated with the presence of five-fold higher amounts of the toxic triphosphated-GCV in EHV4- versus HSV1tk expressing cells. Altogether, these experiments underline the potential advantages of the EHV4tk as a suicide gene.

Kristian Vlahoviček - One of the best experts on this subject based on the ideXlab platform.

  • Distribution of sequence‐dependent curvature in genomic DNA sequences
    FEBS Letters, 1997
    Co-Authors: Andrei Gabrielian, Kristian Vlahoviček, Sándor Pongor
    Abstract:

    The distribution of inherent, sequence-dependent curvature was calculated for a number of prokaryotic (M. genitalium, H. influenzae, M. jannaschii), viral (adenovirus 2, Equine Herpes virus 1), phage (M13, lambda), eukaryotic (S. cerevisiae) and mitochondrial genomes as well as E. coli and human genomic fragments. The genomic averages are in the range of 6–8°/helical turn and only about 20% of DNA is curved less than 3°/helical turn. The prokaryotes and phages appear to have a consistently higher frequency of curved DNA in their genomes than the other genomes tested. Long, highly curved segments, similar to artificially designed curved DNA, are apparently absent from the genomes. Short, curved segments, differing in G+C content may provide environmentally modulated conformational signals for gene regulation. A WWW-server was constructed for the prediction of curved sites from DNA sequences 〈http://icgeb.trieste.it/dna/curve_it.html/〉.

  • Distribution of sequence-dependent curvature in genomic DNA sequences.
    FEBS letters, 1997
    Co-Authors: Andrei Gabrielian, Kristian Vlahoviček, Sándor Pongor
    Abstract:

    The distribution of inherent, sequence-dependent curvature was calculated for a number of prokaryotic (M. genitalium, H. influenzae, M. jannaschii), viral (adenovirus 2, Equine Herpes virus 1), phage (M13, lambda), eukaryotic (S. cerevisiae) and mitochondrial genomes as well as E. coli and human genomic fragments. The genomic averages are in the range of 6-8 degrees/helical turn and only about 20% of DNA is curved less than 3 degrees/helical turn. The prokaryotes and phages appear to have a consistently higher frequency of curved DNA in their genomes than the other genomes tested. Long, highly curved segments, similar to artificially designed curved DNA, are apparently absent from the genomes. Short, curved segments, differing in G+C content may provide environmentally modulated conformational signals for gene regulation. A WWW-server was constructed for the prediction of curved sites from DNA sequences (http://icgeb.trieste.it/dna/curve_it.html/)..