The Experts below are selected from a list of 5595 Experts worldwide ranked by ideXlab platform

Anita Franczak - One of the best experts on this subject based on the ideXlab platform.

  • electromagnetic field emf radiation alters Estrogen Release from the pig myometrium during the peri implantation period
    2021
    Co-Authors: Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Pawel Jozef Wydorski, Anita Franczak
    Abstract:

    An electromagnetic field (EMF) may affect the functions of uterine tissues. This study hypothesized that EMF changes the Estrogenic activity of pig myometrium during the peri-implantation period. Tissue was collected on days 15–16 of the gestation and incubated in the presence of EMF (50 and 120 Hz, 2 and 4 h). The cytochrome P450 aromatase type 3 (CYP19A3) and hydroxysteroid 17β dehydrogenase type 4 (HSD17B4) mRNA transcript abundance, cytochrome P450arom (aromatase), and 17β hydroxysteroid dehydrogenase 17βHSD) protein abundance and estrone (E1) and estradiol-17β (E2) Release were examined using Real-Time PCR, Western blot and radioimmunoassay. Selected myometrial slices were treated with progesterone (P4) to determine whether it functions as a protector against EMF. CYP19A3 mRNA transcript abundance in slices treated with EMF was less at 50 Hz (2 h) and greater at 120 Hz (2 and 4 h). HSD17B4 mRNA transcript was greater in slices treated with EMF at 120 Hz (2 h). Progesterone diminished EMF-related effects on CYP19A3 and HSD17B4. When P4 was added, EMF had suppressive (50 and 120 Hz, 2 h) or enhancing (50 Hz, 4 h) effects on aromatase abundance. The E1 Release was lower after 4 h of EMF treatment at 50 Hz and P4 did not protect myometrial E1 Release. In conclusion, EMF alters the synthesis and Release of E1 and did not affect E2 Release in the myometrium during the peri-implantation period.

  • expression of insulin like growth factor 1 igf 1 and epidermal growth factor egf receptors and the effect of igf 1 and egf on androgen and Estrogen Release in the myometrium of pigs in vitro study
    2020
    Co-Authors: Ewa Monika Waszkiewicz, Wiktoria Kozlowska, Agata Zmijewska, Anita Franczak
    Abstract:

    Porcine myometrium possesses steroidogenic activity but its regulation is not well understood. It was hypothesized that the regulators of myometrial steroidogenesis are insulin-like growth factor 1 (IGF-1) and epidermal growth factor (EGF), which were found to modulate the steroidogenic activity of the endometrium and embryos. Myometrial slices were collected from gravid and nongravid pigs on days 10 or 11, 12 or 13 and 15 or 16 and studied for: (1) the relative abundance of IGF-1R and EGFR mRNA transcripts and proteins, to determine myometrial readiness to response growth factors treatment and (2) the effect of IGF-1 or EGF on the myometrial Release of androstenedione (A4), testosterone (T), estrone (E1) and estradiol-17β (E2). The results showed that the relative expression and abundance of IGF-1R and EGFR in the myometrium were altered regarding the female reproductive status. During the estrous cycle, EGF increased myometrial Release of A4 on days 12-13 and E2 on days 15-16. In gravid pigs (days 15-16), IGF-1 and EGF increased the E1 Release. In conclusion: (1) porcine myometrium possesses the potential to respond to IGF-1 and EGF treatment, (2) EGF significantly increases myometrial A4 and E2 Release in cyclic pigs, while IGF-1 and EGF increase the E1 Release in gravid pigs.

Fei Sun - One of the best experts on this subject based on the ideXlab platform.

  • microrna 224 is involved in transforming growth factor β mediated mouse granulosa cell proliferation and granulosa cell function by targeting smad4
    2010
    Co-Authors: Guidong Yao, Mianmian Yin, Jie Lian, Hui Tian, Lin Liu, Fei Sun
    Abstract:

    Many members of the TGF-β superfamily are indicated to play important roles in ovarian follicular development, such as affecting granulosa cell function and oocyte maturation. Abnormalities associated with TGF-β1 signaling transduction could result in female infertility. MicroRNAs (miRNAs), as small noncoding RNAs, were recently found to regulate gene expression at posttranscriptional levels. However, little is known about the role of miRNAs in TGF-β-mediated granulosa cell proliferation and granulosa cell function. In this study, the miRNA expression profiling was identified from TGF-β1-treated mouse preantral granulosa cells (GCs), and three miRNAs were found to be significantly up-regulated and 13 miRNAs were down-regulated. Among up-regulated miRNAs, miR-224 was the second most significantly elevated miRNA. This up-regulation was attenuated by treatment of GCs with SB431542 (an inhibitor of TGFβ superfamily type I receptors, thus blocking phosphorylation of the downstream effectors Smad2/3), indicating that miR-224 expression was regulated by TGF-β1/Smads pathway. The ectopic expression of miR-224 can enhance TGF-β1-induced GC proliferation through targeting Smad4. Inhibition of endogenous miR-224 partially suppressed GC proliferation induced by TGF-β1. In addition, both miR-224 and TGF-β1 can promote estradiol Release from GC, at least in part, through increasing CYP19A1 mRNA levels. This is the first demonstration that miRNAs can control reproductive functions resulting in promoting TGF-β1-induced GC proliferation and ovarian Estrogen Release. Such miRNA-mediated effects could be potentially used for regulation of reproductive processes or for treatment of reproductive disorders.

Agata Zmijewska - One of the best experts on this subject based on the ideXlab platform.

  • electromagnetic field emf radiation alters Estrogen Release from the pig myometrium during the peri implantation period
    2021
    Co-Authors: Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Pawel Jozef Wydorski, Anita Franczak
    Abstract:

    An electromagnetic field (EMF) may affect the functions of uterine tissues. This study hypothesized that EMF changes the Estrogenic activity of pig myometrium during the peri-implantation period. Tissue was collected on days 15–16 of the gestation and incubated in the presence of EMF (50 and 120 Hz, 2 and 4 h). The cytochrome P450 aromatase type 3 (CYP19A3) and hydroxysteroid 17β dehydrogenase type 4 (HSD17B4) mRNA transcript abundance, cytochrome P450arom (aromatase), and 17β hydroxysteroid dehydrogenase 17βHSD) protein abundance and estrone (E1) and estradiol-17β (E2) Release were examined using Real-Time PCR, Western blot and radioimmunoassay. Selected myometrial slices were treated with progesterone (P4) to determine whether it functions as a protector against EMF. CYP19A3 mRNA transcript abundance in slices treated with EMF was less at 50 Hz (2 h) and greater at 120 Hz (2 and 4 h). HSD17B4 mRNA transcript was greater in slices treated with EMF at 120 Hz (2 h). Progesterone diminished EMF-related effects on CYP19A3 and HSD17B4. When P4 was added, EMF had suppressive (50 and 120 Hz, 2 h) or enhancing (50 Hz, 4 h) effects on aromatase abundance. The E1 Release was lower after 4 h of EMF treatment at 50 Hz and P4 did not protect myometrial E1 Release. In conclusion, EMF alters the synthesis and Release of E1 and did not affect E2 Release in the myometrium during the peri-implantation period.

  • expression of insulin like growth factor 1 igf 1 and epidermal growth factor egf receptors and the effect of igf 1 and egf on androgen and Estrogen Release in the myometrium of pigs in vitro study
    2020
    Co-Authors: Ewa Monika Waszkiewicz, Wiktoria Kozlowska, Agata Zmijewska, Anita Franczak
    Abstract:

    Porcine myometrium possesses steroidogenic activity but its regulation is not well understood. It was hypothesized that the regulators of myometrial steroidogenesis are insulin-like growth factor 1 (IGF-1) and epidermal growth factor (EGF), which were found to modulate the steroidogenic activity of the endometrium and embryos. Myometrial slices were collected from gravid and nongravid pigs on days 10 or 11, 12 or 13 and 15 or 16 and studied for: (1) the relative abundance of IGF-1R and EGFR mRNA transcripts and proteins, to determine myometrial readiness to response growth factors treatment and (2) the effect of IGF-1 or EGF on the myometrial Release of androstenedione (A4), testosterone (T), estrone (E1) and estradiol-17β (E2). The results showed that the relative expression and abundance of IGF-1R and EGFR in the myometrium were altered regarding the female reproductive status. During the estrous cycle, EGF increased myometrial Release of A4 on days 12-13 and E2 on days 15-16. In gravid pigs (days 15-16), IGF-1 and EGF increased the E1 Release. In conclusion: (1) porcine myometrium possesses the potential to respond to IGF-1 and EGF treatment, (2) EGF significantly increases myometrial A4 and E2 Release in cyclic pigs, while IGF-1 and EGF increase the E1 Release in gravid pigs.

Wiktoria Kozlowska - One of the best experts on this subject based on the ideXlab platform.

  • electromagnetic field emf radiation alters Estrogen Release from the pig myometrium during the peri implantation period
    2021
    Co-Authors: Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Pawel Jozef Wydorski, Anita Franczak
    Abstract:

    An electromagnetic field (EMF) may affect the functions of uterine tissues. This study hypothesized that EMF changes the Estrogenic activity of pig myometrium during the peri-implantation period. Tissue was collected on days 15–16 of the gestation and incubated in the presence of EMF (50 and 120 Hz, 2 and 4 h). The cytochrome P450 aromatase type 3 (CYP19A3) and hydroxysteroid 17β dehydrogenase type 4 (HSD17B4) mRNA transcript abundance, cytochrome P450arom (aromatase), and 17β hydroxysteroid dehydrogenase 17βHSD) protein abundance and estrone (E1) and estradiol-17β (E2) Release were examined using Real-Time PCR, Western blot and radioimmunoassay. Selected myometrial slices were treated with progesterone (P4) to determine whether it functions as a protector against EMF. CYP19A3 mRNA transcript abundance in slices treated with EMF was less at 50 Hz (2 h) and greater at 120 Hz (2 and 4 h). HSD17B4 mRNA transcript was greater in slices treated with EMF at 120 Hz (2 h). Progesterone diminished EMF-related effects on CYP19A3 and HSD17B4. When P4 was added, EMF had suppressive (50 and 120 Hz, 2 h) or enhancing (50 Hz, 4 h) effects on aromatase abundance. The E1 Release was lower after 4 h of EMF treatment at 50 Hz and P4 did not protect myometrial E1 Release. In conclusion, EMF alters the synthesis and Release of E1 and did not affect E2 Release in the myometrium during the peri-implantation period.

  • expression of insulin like growth factor 1 igf 1 and epidermal growth factor egf receptors and the effect of igf 1 and egf on androgen and Estrogen Release in the myometrium of pigs in vitro study
    2020
    Co-Authors: Ewa Monika Waszkiewicz, Wiktoria Kozlowska, Agata Zmijewska, Anita Franczak
    Abstract:

    Porcine myometrium possesses steroidogenic activity but its regulation is not well understood. It was hypothesized that the regulators of myometrial steroidogenesis are insulin-like growth factor 1 (IGF-1) and epidermal growth factor (EGF), which were found to modulate the steroidogenic activity of the endometrium and embryos. Myometrial slices were collected from gravid and nongravid pigs on days 10 or 11, 12 or 13 and 15 or 16 and studied for: (1) the relative abundance of IGF-1R and EGFR mRNA transcripts and proteins, to determine myometrial readiness to response growth factors treatment and (2) the effect of IGF-1 or EGF on the myometrial Release of androstenedione (A4), testosterone (T), estrone (E1) and estradiol-17β (E2). The results showed that the relative expression and abundance of IGF-1R and EGFR in the myometrium were altered regarding the female reproductive status. During the estrous cycle, EGF increased myometrial Release of A4 on days 12-13 and E2 on days 15-16. In gravid pigs (days 15-16), IGF-1 and EGF increased the E1 Release. In conclusion: (1) porcine myometrium possesses the potential to respond to IGF-1 and EGF treatment, (2) EGF significantly increases myometrial A4 and E2 Release in cyclic pigs, while IGF-1 and EGF increase the E1 Release in gravid pigs.

Guidong Yao - One of the best experts on this subject based on the ideXlab platform.

  • microrna 224 is involved in transforming growth factor β mediated mouse granulosa cell proliferation and granulosa cell function by targeting smad4
    2010
    Co-Authors: Guidong Yao, Mianmian Yin, Jie Lian, Hui Tian, Lin Liu, Fei Sun
    Abstract:

    Many members of the TGF-β superfamily are indicated to play important roles in ovarian follicular development, such as affecting granulosa cell function and oocyte maturation. Abnormalities associated with TGF-β1 signaling transduction could result in female infertility. MicroRNAs (miRNAs), as small noncoding RNAs, were recently found to regulate gene expression at posttranscriptional levels. However, little is known about the role of miRNAs in TGF-β-mediated granulosa cell proliferation and granulosa cell function. In this study, the miRNA expression profiling was identified from TGF-β1-treated mouse preantral granulosa cells (GCs), and three miRNAs were found to be significantly up-regulated and 13 miRNAs were down-regulated. Among up-regulated miRNAs, miR-224 was the second most significantly elevated miRNA. This up-regulation was attenuated by treatment of GCs with SB431542 (an inhibitor of TGFβ superfamily type I receptors, thus blocking phosphorylation of the downstream effectors Smad2/3), indicating that miR-224 expression was regulated by TGF-β1/Smads pathway. The ectopic expression of miR-224 can enhance TGF-β1-induced GC proliferation through targeting Smad4. Inhibition of endogenous miR-224 partially suppressed GC proliferation induced by TGF-β1. In addition, both miR-224 and TGF-β1 can promote estradiol Release from GC, at least in part, through increasing CYP19A1 mRNA levels. This is the first demonstration that miRNAs can control reproductive functions resulting in promoting TGF-β1-induced GC proliferation and ovarian Estrogen Release. Such miRNA-mediated effects could be potentially used for regulation of reproductive processes or for treatment of reproductive disorders.