The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform

Hermann Bujard - One of the best experts on this subject based on the ideXlab platform.

  • control of gene activity in higher Eukaryotic Cells by prokaryotic regulatory elements
    Trends in Biotechnology, 1994
    Co-Authors: Manfred Gossen, Angelika L Bonin, Hermann Bujard
    Abstract:

    Abstract Regulated gene expression systems for the study of gene function in prokaryotes and yeast have been available for several years. By contrast, comparable systems in higher organisms have proven to be problematic due to their high complexity. Recently, regulatory proteins from distant species have been used to establish highly specific control systems in Eukaryotic Cells. This is possible because their action can be modulated by effectors that are inert to the physiology of the cell or organism and therefore do not elicit pleiotropic effects. Such monospecific regulatory circuits open up new possibilities for the study of gene function in vivo.

  • control of gene activity in higher Eukaryotic Cells by prokaryotic regulatory elements
    Trends in Biochemical Sciences, 1993
    Co-Authors: Manfred Gossen, Angelika L Bonin, Hermann Bujard
    Abstract:

    Regulated gene expression systems for the study of gene function in prokaryotes and yeast have been available for several years. However, comparable systems in higher organisms are more complex and problematic. Recently, regulatory proteins from distant species have been used to establish highly specific control systems in Eukaryotic Cells. This is possible because their action can be modulated by effectors that are inert to the physiology of the organism or cell and therefore do not elicit pleiotropic effects. Such monospecific regulatory circuits open up new possibilities for the study of gene function in vivo.

Manfred Gossen - One of the best experts on this subject based on the ideXlab platform.

  • control of gene activity in higher Eukaryotic Cells by prokaryotic regulatory elements
    Trends in Biotechnology, 1994
    Co-Authors: Manfred Gossen, Angelika L Bonin, Hermann Bujard
    Abstract:

    Abstract Regulated gene expression systems for the study of gene function in prokaryotes and yeast have been available for several years. By contrast, comparable systems in higher organisms have proven to be problematic due to their high complexity. Recently, regulatory proteins from distant species have been used to establish highly specific control systems in Eukaryotic Cells. This is possible because their action can be modulated by effectors that are inert to the physiology of the cell or organism and therefore do not elicit pleiotropic effects. Such monospecific regulatory circuits open up new possibilities for the study of gene function in vivo.

  • control of gene activity in higher Eukaryotic Cells by prokaryotic regulatory elements
    Trends in Biochemical Sciences, 1993
    Co-Authors: Manfred Gossen, Angelika L Bonin, Hermann Bujard
    Abstract:

    Regulated gene expression systems for the study of gene function in prokaryotes and yeast have been available for several years. However, comparable systems in higher organisms are more complex and problematic. Recently, regulatory proteins from distant species have been used to establish highly specific control systems in Eukaryotic Cells. This is possible because their action can be modulated by effectors that are inert to the physiology of the organism or cell and therefore do not elicit pleiotropic effects. Such monospecific regulatory circuits open up new possibilities for the study of gene function in vivo.

Graham D Pavitt - One of the best experts on this subject based on the ideXlab platform.

  • protein synthesis initiation in Eukaryotic Cells
    Cold Spring Harbor Perspectives in Biology, 2018
    Co-Authors: William C Merrick, Graham D Pavitt
    Abstract:

    This review summarizes our current understanding of the major pathway for the initiation phase of protein synthesis in Eukaryotic Cells, with a focus on recent advances. We describe the major scanning or messenger RNA (mRNA) m7G cap-dependent mechanism, which is a highly coordinated and stepwise regulated process that requires the combined action of at least 12 distinct translation factors with initiator transfer RNA (tRNA), ribosomes, and mRNAs. We limit our review to studies involving either mammalian or budding yeast Cells and factors, as these represent the two best-studied experimental systems, and only include a reference to other organisms where particular insight has been gained. We close with a brief description of what we feel are some of the major unknowns in Eukaryotic initiation.

Virginia M Pain - One of the best experts on this subject based on the ideXlab platform.

  • initiation of protein synthesis in Eukaryotic Cells
    FEBS Journal, 1996
    Co-Authors: Virginia M Pain
    Abstract:

    It is becoming increasingly apparent that translational control plays an important role in the regulation of gene expression in Eukaryotic Cells. Most of the known physiological effects on translation are exerted at the level of polypeptide chain initiation. Research on initiation of translation over the past five years has yielded much new information, which can be divided into three main areas: (a) structure and function of initiation factors (including identification by sequencing studies of consensus domains and motifs) and investigation of protein-protein and protein-RNA interactions during initiation; (b) physiological regulation of initiation factor activities and (c) identification of features in the 5′ and 3′ untranslated regions of messenger RNA molecules that regulate the selection of these mRNAs for translation. This review aims to assess recent progress in these three areas and to explore their interrelationships.

Angelika L Bonin - One of the best experts on this subject based on the ideXlab platform.

  • control of gene activity in higher Eukaryotic Cells by prokaryotic regulatory elements
    Trends in Biotechnology, 1994
    Co-Authors: Manfred Gossen, Angelika L Bonin, Hermann Bujard
    Abstract:

    Abstract Regulated gene expression systems for the study of gene function in prokaryotes and yeast have been available for several years. By contrast, comparable systems in higher organisms have proven to be problematic due to their high complexity. Recently, regulatory proteins from distant species have been used to establish highly specific control systems in Eukaryotic Cells. This is possible because their action can be modulated by effectors that are inert to the physiology of the cell or organism and therefore do not elicit pleiotropic effects. Such monospecific regulatory circuits open up new possibilities for the study of gene function in vivo.

  • control of gene activity in higher Eukaryotic Cells by prokaryotic regulatory elements
    Trends in Biochemical Sciences, 1993
    Co-Authors: Manfred Gossen, Angelika L Bonin, Hermann Bujard
    Abstract:

    Regulated gene expression systems for the study of gene function in prokaryotes and yeast have been available for several years. However, comparable systems in higher organisms are more complex and problematic. Recently, regulatory proteins from distant species have been used to establish highly specific control systems in Eukaryotic Cells. This is possible because their action can be modulated by effectors that are inert to the physiology of the organism or cell and therefore do not elicit pleiotropic effects. Such monospecific regulatory circuits open up new possibilities for the study of gene function in vivo.