The Experts below are selected from a list of 1944 Experts worldwide ranked by ideXlab platform
F. Martin-laurent - One of the best experts on this subject based on the ideXlab platform.
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Microalgae community structure analysis based on 18S rDNA amplification from DNA extracted directly from soil as a potential soil bioindicator
Agronomy for Sustainable Development, 2005Co-Authors: A. Bérard, U. Dorigo, J.f. Humbert, F. Martin-laurentAbstract:Soil algae are photosynthetically active microorganisms showing changeable community structure, depending on the soil type, the agricultural practices and the application of pesticides. To characterise algal community structure, molecular approaches complementary to classical microbiological approaches based on the isolation and the culture of soil algae are required. Our study describes a polymerase chain reaction (PCR) approach targeting algal 18S rDNA sequences of desoxyribonucleic acid (DNA) samples extracted either from unialgal Eukaryotic Microalgae culture, complex assemblages of Microalgae populations or natural soil communities. Our first results showed that Microalgae rDNA can be amplified by PCR from soil DNA samples. They also indicated difficulties extracting DNA from diatoms directly from soils, probably because of the presence of robust silicate valves. An 18S rDNA library has been established and preliminary phylogenetic analysis showed the feasibility of applying molecular methods to studying edaphic algae community structure. This is promising for soil algae ecology and for developing soil biological indicators.
Kyle J. Lauersen - One of the best experts on this subject based on the ideXlab platform.
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Eukaryotic Microalgae as hosts for light-driven heterologous isoprenoid production
Planta, 2019Co-Authors: Kyle J. LauersenAbstract:Main conclusionsEukaryotic Microalgae hold incredible metabolic potential for the sustainable production of heterologous isoprenoid products. Recent advances in algal engineering have enabled the demonstration of prominent examples of heterologous isoprenoid production. Isoprenoids, also known as terpenes or terpenoids, are the largest class of natural chemicals, with a vast diversity of structures and biological roles. Some have high-value in human-use applications, although may be found in their native contexts in low abundance or be difficult to extract and purify. Heterologous production of isoprenoid compounds in heterotrophic microbial hosts such as bacteria or yeasts has been an active area of research for some time and is now a mature technology. Eukaryotic Microalgae represent sustainable alternatives to these hosts for biotechnological production processes as their cultivation can be driven by light and freely available CO_2 as a carbon source. Their photosynthetic lifestyles require metabolic architectures structured towards the generation of associated isoprenoids (carotenoids, phytol) which participate in photon capture, energy dissipation, and electron transfer. Eukaryotic Microalgae should, therefore, contain inherently high capacities for the generation of heterologous isoprenoid products. Although engineering strategies in Eukaryotic Microalgae have lagged behind the more genetically tractable bacteria and yeasts, recent advances in algal engineering concepts have demonstrated prominent examples of light-driven heterologous isoprenoid production from these photosynthetic hosts. This work seeks to provide practical insights into the choice of Eukaryotic Microalgae as biotechnological chassis. Recent reports of advances in algal engineering for heterologous isoprenoid production are highlighted as encouraging examples that promote their expanded use as sustainable green-cell factories. Current state of the art, limitations, and future challenges are also discussed.Graphical abstract
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Engineered Fusion Proteins for Efficient Protein Secretion and Purification of a Human Growth Factor from the Green Microalga Chlamydomonas reinhardtii.
ACS Synthetic Biology, 2018Co-Authors: Thomas Baier, Kyle J. Lauersen, Dana Kros, Rebecca C. Feiner, Kristian M. Müller, Olaf KruseAbstract:Light-driven recombinant protein (RP) production in Eukaryotic Microalgae offers a sustainable alternative to other established cell-culture systems. RP production via secretion into the culture medium enables simple product separation from the cells adding a layer of process value in addition to the algal biomass, which can be separately harvested. For the model microalga Chlamydomonas reinhardtii, a broad range of molecular tools have been established to enable heterologous gene expression; however, low RP production levels and unreliable purification from secretion concepts have been reported. Domesticated C. reinhardtii strains used for genetic engineering are often cell-wall deficient. These strains nevertheless secrete cell-wall components such as insoluble (hydroxy)proline-rich glycoproteins into the culture media, which hinder downstream purification processes. Here, we attempted to overcome limitations in secretion titers and improve protein purification by combining fusion partners that enhance ...
Klaas J Hellingwerf - One of the best experts on this subject based on the ideXlab platform.
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potential of industrial biotechnology with cyanobacteria and Eukaryotic Microalgae
Current Opinion in Biotechnology, 2013Co-Authors: Rene H Wijffels, Olaf Kruse, Klaas J HellingwerfAbstract:Both cyanobacteria and Eukaryotic Microalgae are promising organisms for sustainable production of bulk products such as food, feed, materials, chemicals and fuels. In this review we will summarize the potential and current biotechnological developments. Cyanobacteria are promising host organisms for the production of small molecules that can be secreted such as ethanol, butanol, fatty acids and other organic acids. Eukaryotic Microalgae are interesting for products for which cellular storage is important such as proteins, lipids, starch and alkanes. For the development of new and promising lines of production, strains of both cyanobacteria and Eukaryotic Microalgae have to be improved. Transformation systems have been much better developed in cyanobacteria. However, several products would be preferably produced with Eukaryotic Microalgae. In the case of cyanobacteria a synthetic-systems biology approach has a great potential to exploit cyanobacteria as cell factories. For Eukaryotic Microalgae transformation systems need to be further developed. A promising strategy is transformation of heterologous (prokaryotic and Eukaryotic) genes in established Eukaryotic hosts such as Chlamydomonas reinhardtii. Experimental outdoor pilots under containment for the production of genetically modified cyanobacteria and Microalgae are in progress. For full scale production risks of release of genetically modified organisms need to be assessed.
Olaf Kruse - One of the best experts on this subject based on the ideXlab platform.
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Engineered Fusion Proteins for Efficient Protein Secretion and Purification of a Human Growth Factor from the Green Microalga Chlamydomonas reinhardtii.
ACS Synthetic Biology, 2018Co-Authors: Thomas Baier, Kyle J. Lauersen, Dana Kros, Rebecca C. Feiner, Kristian M. Müller, Olaf KruseAbstract:Light-driven recombinant protein (RP) production in Eukaryotic Microalgae offers a sustainable alternative to other established cell-culture systems. RP production via secretion into the culture medium enables simple product separation from the cells adding a layer of process value in addition to the algal biomass, which can be separately harvested. For the model microalga Chlamydomonas reinhardtii, a broad range of molecular tools have been established to enable heterologous gene expression; however, low RP production levels and unreliable purification from secretion concepts have been reported. Domesticated C. reinhardtii strains used for genetic engineering are often cell-wall deficient. These strains nevertheless secrete cell-wall components such as insoluble (hydroxy)proline-rich glycoproteins into the culture media, which hinder downstream purification processes. Here, we attempted to overcome limitations in secretion titers and improve protein purification by combining fusion partners that enhance ...
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CHAPTER 6:Sustaining Hydrogen Production in Eukaryotic Microalgae Through Genetic Approaches
Comprehensive Series in Photochemical & Photobiological Sciences, 2018Co-Authors: Olaf Kruse, Deepak VenkannaAbstract:The efficiency of H2 production with Microalgae is generally hampered by the fact that under in vivo conditions the bio-catalytic conversion of protons and electrons to hydrogen is only used as a limited valve system, reacting to severe stress conditions to prevent cell damage caused by an imbalance of the cellular redox-homeostasis. The participating hydrogenase enzyme in Eukaryotic Microalgae has a large enzymatic capacity; however, high H2-production rates are mainly prevented by insufficient delivery of protons and electrons. To overcome this bottleneck, molecular engineering of specific parts of the cellular metabolism has been considered as a promising approach. Recent achievements using forward and reverse genetic approaches for the identification and construction of efficient, Eukaryotic-microalgal H2 producers resulted in the isolation of high H2 producers with production rates up to 850 ml H2 l−1 cell culture. Application strategies include systematic genomics to identify and isolate new, high H2, GMO-cell lines through different screening programs from libraries constructed by random mutagenesis, as well as targeted mutagenesis approaches combined with systems biology required for the localization of potential bottlenecks.
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potential of industrial biotechnology with cyanobacteria and Eukaryotic Microalgae
Current Opinion in Biotechnology, 2013Co-Authors: Rene H Wijffels, Olaf Kruse, Klaas J HellingwerfAbstract:Both cyanobacteria and Eukaryotic Microalgae are promising organisms for sustainable production of bulk products such as food, feed, materials, chemicals and fuels. In this review we will summarize the potential and current biotechnological developments. Cyanobacteria are promising host organisms for the production of small molecules that can be secreted such as ethanol, butanol, fatty acids and other organic acids. Eukaryotic Microalgae are interesting for products for which cellular storage is important such as proteins, lipids, starch and alkanes. For the development of new and promising lines of production, strains of both cyanobacteria and Eukaryotic Microalgae have to be improved. Transformation systems have been much better developed in cyanobacteria. However, several products would be preferably produced with Eukaryotic Microalgae. In the case of cyanobacteria a synthetic-systems biology approach has a great potential to exploit cyanobacteria as cell factories. For Eukaryotic Microalgae transformation systems need to be further developed. A promising strategy is transformation of heterologous (prokaryotic and Eukaryotic) genes in established Eukaryotic hosts such as Chlamydomonas reinhardtii. Experimental outdoor pilots under containment for the production of genetically modified cyanobacteria and Microalgae are in progress. For full scale production risks of release of genetically modified organisms need to be assessed.
Karen P. Fawley - One of the best experts on this subject based on the ideXlab platform.
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Identification of Eukaryotic microalgal strains
Journal of Applied Phycology, 2020Co-Authors: Marvin W. Fawley, Karen P. FawleyAbstract:Proper identification and documentation of Microalgae is often lacking in publications of applied phycology, algal physiology and biochemistry. Identification of many Eukaryotic Microalgae can be very daunting to the nonspecialist. We present a systematic process for identifying Eukaryotic Microalgae using morphological evidence and DNA sequence analysis. Our intent was to provide an identification method that could be used by nontaxonomists, but which is grounded in the current techniques used by algal taxonomists. Central to the identification is database searches with DNA sequences of appropriate loci. We provide usable criteria for identification at the genus or species level, depending on the availability of sequence data in curated databases and repositories. Particular attention is paid to dealing with possible misidentifications in DNA databases and utilizing current taxonomy.