The Experts below are selected from a list of 579 Experts worldwide ranked by ideXlab platform
Richard D.m. Nash - One of the best experts on this subject based on the ideXlab platform.
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Linking spawning ground extent to environmental factors - patterns and dispersal during the egg phase of four North Sea fishes
Canadian Journal of Fisheries and Aquatic Sciences, 2018Co-Authors: Hannes Höffle, Richard D.m. Nash, Clive Fox, Sandrine Vaz, Cindy J. G. Van Damme, Stephanie Lelievre, Christophe Loots, Peter J. Wright, Peter MunkAbstract:Previous studies have shown that four commercially important demersal species, namely Atlantic cod (Gadus morhua), haddock (Melanogrammus aeglefinus), whiting (Merlangius merlangus), and European Plaice (Pleuronectes platessa), spawn in distinct areas across the North Sea. Based on two comprehensive ichthyoplankton surveys in 2004 and 2009, the present study uses generalized additive mixed models to delimit these spawning grounds using the distribution of recently spawned eggs, investigates their relationship to specific environmental conditions, and examines egg dispersal during their development. Results indicate that presence–absence of early stage eggs is more related to temporal and topographic variables, while egg densities are closely linked with hydrography. Egg distribution patterns were relatively consistent during development and only changed near hatching. Compared with historic observations, the location of the spawning grounds appeared stable on the broad scale but centres of egg abundance v...
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Linking spawning ground extent to environmental factors - patterns and dispersal during the egg phase of four North Sea fishes
Canadian Journal of Fisheries and Aquatic Sciences, 2018Co-Authors: Hannes Höffle, Richard D.m. Nash, Clive Fox, Sandrine Vaz, Cindy J. G. Van Damme, Stephanie Lelievre, Christophe Loots, Peter J. Wright, Peter MunkAbstract:Previous studies have shown that four commercially important demersal species, namely Atlantic cod (Gadus morhua), haddock (Melanogrammus aeglefinus), whiting (Merlangius merlangus), and European Plaice (Pleuronectes platessa), spawn in distinct areas across the North Sea. Based on two comprehensive ichthyoplankton surveys in 2004 and 2009, the present study uses generalized additive mixed models to delimit these spawning grounds using the distribution of recently spawned eggs, investigates their relationship to specific environmental conditions, and examines egg dispersal during their development. Results indicate that presence-absence of early stage eggs is more related to temporal and topographic variables, while egg densities are closely linked with hydrography. Egg distribution patterns were relatively consistent during development and only changed near hatching. Compared with historic observations, the location of the spawning grounds appeared stable on the broad scale but centres of egg abundance varied between the surveyed years. Potential effects of long-term climate change and anthropogenic short-term disturbances, such as seismic surveys, on fish reproduction are discussed, pointing out the demand for multispecies studies on these issues.
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Growth dynamics of European Plaice Pleuronectes platessa L. in nursery areas: A review
Journal of Sea Research, 2014Co-Authors: Benjamin J. Ciotti, Richard D.m. Nash, Timothy E. Targett, Audrey J GeffenAbstract:Young-of-the-year European Plaice Pleuronectes platessa L. (hereafter: ‘YOY Plaice’) in shallow, sandy areas is a long- and intensively-studied species and an ideal model for understanding growth dynamics in fish nurseries. In order to provide an overview of and access point to this rich literature and to guide future research on juvenile fish growth dynamics, we review patterns of growth variation in YOY Plaice following settlement and evaluate evidence for underlying causes, including maximum growth, temperature, prey conditions and competition. A decline in growth rate during late summer and autumn was the clearest and most widespread pattern, but was not clearly related to any of the potential causes previously considered. Interannual growth variation was substantial and despite evidence that intraspecific competition was responsible, other possible causes were also supported and others were only rarely assessed. Growth also varied considerably at a range of spatial scales (100s of m–100s of km). Causes of small-scale ( 200 km) growth variation remain poorly understood and while intermediate-scale growth variation has been related to prey conditions and intraspecific competition, the role of interspecific competition requires further investigation. Therefore, despite clear evidence for growth heterogeneity at numerous spatiotemporal scales, underlying causes remain elusive. We highlight some principal challenges to measuring and understanding the complex and scale-dependent causes of growth variation. To overcome these challenges, and therefore resolve important nursery processes for juvenile fish, we recommend more detailed and spatiotemporally explicit investigations of growth, metabolic processes and physiological energetics in situ; a focus on possible proximate and ultimate factors driving these dynamics; and development of new hypotheses to explain growth variation starting with general physical features that define nursery environments.
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Small-scale spatial and temporal heterogeneity in growth and condition of juvenile fish on sandy beaches
Journal of Experimental Marine Biology and Ecology, 2013Co-Authors: Benjamin J. Ciotti, Richard D.m. Nash, Timothy E. Targett, Michael T. BurrowsAbstract:To characterize fine-grained heterogeneity in the functional role of sandy beaches as fish nurseries, we measured RNA concentration, DNA concentration and RNA-predicted growth rate in young-of-the-year European Plaice Pleuronectes platessa L. (‘YOY Plaice’) at beaches on the western side of the British Isles at small alongshore (100–500 m), temporal (days to weeks), depth (0.5–2 m below waterline) and inter-individual scales. We compared variations in these nucleic-acid-based metrics of condition and growth with a null metric (total length) considered to be less responsive to short-term changes in environmental conditions. All four metrics varied at small spatial and temporal scales, particularly RNA-based metrics of short-term condition and growth. A decline in condition and growth during summer was the most important source of temporal variation. Condition and growth also increased with depth below waterline. Alongshore variations within a beach were relatively small, but variance among stations on the same beach (500 m scale) was similar in magnitude to variance among beaches separated by 100 km. High variance in nucleic acid concentrations among samples resulted not only from technical error but also from inter-individual variation. Our application of high-resolution biochemical indices therefore demonstrates that functional roles of sandy beaches can be structured at remarkably small spatial (500 m) and temporal (days) scales, within a single beach.
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Mortality through the early life-history of fish: What can we learn from European Plaice (Pleuronectes platessa L.)?
Journal of Marine Systems, 2012Co-Authors: Richard D.m. Nash, Audrey J GeffenAbstract:Abstract It is difficult to estimate natural mortality for many marine fish populations, especially during the transition period from larvae to juveniles, because the appropriate data are scarce. Plaice (Pleuronectes platessa) is an exception since it has been studied extensively. The study of mortality rates in juveniles is made easier because the nursery grounds are inshore and generally less than 5 m deep. This contribution considers the factors affecting mortality rates for eggs and larvae, and for settlement and nursery ground phases. There are problems associated with estimating mortality rates, especially for juveniles because immigration into nursery areas at the beginning of the season and emigration of larger individuals off nursery grounds in the latter part of the season confound losses due to mortality. The shifts in mortality schedules and the causes through the early life history are investigated in relation to concepts such as ‘nursery ground carrying capacity’ and ‘self-thinning’. Predation in the pelagic phase is probably density independent and a source of inter-annual variability in survival of early life-history stages. Predation mortality during the nursery ground phase is most likely density dependent. However, there is a need for further in-depth study, especially during the period of settlement.
T L Catchpole - One of the best experts on this subject based on the ideXlab platform.
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survival of European Plaice discarded from coastal otter trawl fisheries in the english channel
Journal of Environmental Management, 2017Co-Authors: Marie Morfin, Dorothee Kopp, Hugues P Benoit, Sonia Mehault, Peter Randall, Robert Foster, T L CatchpoleAbstract:Abstract Species that have a high likelihood of surviving the discarding process have become great concern since the European Union reformed the Common Fisheries Policy and enacted a landing obligation prohibiting the discarding any individuals of species under quota. Among species presenting an elevated survival potential, Plaice ( Pleuronectes platessa ) is one of the most discarded in the coastal otter trawl fishery in the English Channel. The objective of this study is to provide the most reliable estimates of Plaice survival after release in commercial conditions, and to identify the factors that influence survival rates. A captivity experiment was conducted in January–February in the English fishery to assess the survival of discarded Plaice as a function of a semi-quantitative index of fish vitality, which has been demonstrated to be a good proxy of fish survival in comparable fishing and environmental conditions. This study examined the potential of this index to estimate discard survival in three trials from the English and French fisheries and at three different seasons. The vitality index was then used to analyse the influence of several factors (fishing practices, environmental conditions and fish biological characteristics) on the discard survival. The survival rates for Plaice were accurately estimated at 62.8% in January–February, 66.6% in November and 45.2% in July. While these rates remained substantial whatever the fishing, environmental or fish biological conditions, the time fish spent on the deck, the bottom and air temperatures, the tow depth and the fish length had a significant influence on Plaice survival. In practice, Plaice survival could be enhanced by releasing the fish early during catch sorting and avoiding exposure to extreme air temperatures.
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Survival of European Plaice discarded from coastal otter trawl fisheries in the English Channel.
Journal of Environmental Management, 2017Co-Authors: Marie Morfin, Dorothee Kopp, Hugues P Benoit, Sonia Mehault, Peter Randall, Robert Foster, T L CatchpoleAbstract:Abstract Species that have a high likelihood of surviving the discarding process have become great concern since the European Union reformed the Common Fisheries Policy and enacted a landing obligation prohibiting the discarding any individuals of species under quota. Among species presenting an elevated survival potential, Plaice ( Pleuronectes platessa ) is one of the most discarded in the coastal otter trawl fishery in the English Channel. The objective of this study is to provide the most reliable estimates of Plaice survival after release in commercial conditions, and to identify the factors that influence survival rates. A captivity experiment was conducted in January–February in the English fishery to assess the survival of discarded Plaice as a function of a semi-quantitative index of fish vitality, which has been demonstrated to be a good proxy of fish survival in comparable fishing and environmental conditions. This study examined the potential of this index to estimate discard survival in three trials from the English and French fisheries and at three different seasons. The vitality index was then used to analyse the influence of several factors (fishing practices, environmental conditions and fish biological characteristics) on the discard survival. The survival rates for Plaice were accurately estimated at 62.8% in January–February, 66.6% in November and 45.2% in July. While these rates remained substantial whatever the fishing, environmental or fish biological conditions, the time fish spent on the deck, the bottom and air temperatures, the tow depth and the fish length had a significant influence on Plaice survival. In practice, Plaice survival could be enhanced by releasing the fish early during catch sorting and avoiding exposure to extreme air temperatures.
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Discarded fish in European waters: general patterns and contrasts
Ices Journal of Marine Science, 2013Co-Authors: S.s. Uhlmann, T L Catchpole, Aloysius T. M. Van Helmond, Elísabet Kemp Stefánsdóttir, Sigríður Sigurðardóttir, John Haralabous, Jose M. Bellido, Aina Carbonell, Dimitrios Damalas, Laurence FauconnetAbstract:To reduce the practice of discarding commercially fished organisms, several measures such as a discard ban and extra allowances on top of landings quotas (“catch quota”) have been proposed by the European Commission. However, for their development and successful implementation, an understanding of discard patterns on a European scale is needed. In this study, we present an inter-national synthesis of discard data collected on board commercial, towed-gear equipped vessels operating under six different national flags spanning from the Baltic to the Mediterranean Seas mainly between 2003 and 2008. We considered discarded species of commercial value such as Atlantic cod (Gadus morhua), haddock (Melanogrammus aeglefinus), European hake (Merluccius merluccius), and European Plaice (Pleuronectes platessa). Comparisons of discard per unit effort rates expressed as numbers per hour of fishing revealed that in the Mediterranean Sea minimum size-regulated species such as hake are generally discarded in much lower numbers than elsewhere. For most species examined, variability in discard rates across regions was greater than across fisheries, suggesting that a region-by-region approach to discard reduction would be more relevant. The high uncertainty in discard rate estimates suggests that current sampling regimes should be either expanded or complemented by other data sources, if they are to be used for setting catch quotas.
Clive Fox - One of the best experts on this subject based on the ideXlab platform.
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Linking spawning ground extent to environmental factors - patterns and dispersal during the egg phase of four North Sea fishes
Canadian Journal of Fisheries and Aquatic Sciences, 2018Co-Authors: Hannes Höffle, Richard D.m. Nash, Clive Fox, Sandrine Vaz, Cindy J. G. Van Damme, Stephanie Lelievre, Christophe Loots, Peter J. Wright, Peter MunkAbstract:Previous studies have shown that four commercially important demersal species, namely Atlantic cod (Gadus morhua), haddock (Melanogrammus aeglefinus), whiting (Merlangius merlangus), and European Plaice (Pleuronectes platessa), spawn in distinct areas across the North Sea. Based on two comprehensive ichthyoplankton surveys in 2004 and 2009, the present study uses generalized additive mixed models to delimit these spawning grounds using the distribution of recently spawned eggs, investigates their relationship to specific environmental conditions, and examines egg dispersal during their development. Results indicate that presence-absence of early stage eggs is more related to temporal and topographic variables, while egg densities are closely linked with hydrography. Egg distribution patterns were relatively consistent during development and only changed near hatching. Compared with historic observations, the location of the spawning grounds appeared stable on the broad scale but centres of egg abundance varied between the surveyed years. Potential effects of long-term climate change and anthropogenic short-term disturbances, such as seismic surveys, on fish reproduction are discussed, pointing out the demand for multispecies studies on these issues.
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Linking spawning ground extent to environmental factors - patterns and dispersal during the egg phase of four North Sea fishes
Canadian Journal of Fisheries and Aquatic Sciences, 2018Co-Authors: Hannes Höffle, Richard D.m. Nash, Clive Fox, Sandrine Vaz, Cindy J. G. Van Damme, Stephanie Lelievre, Christophe Loots, Peter J. Wright, Peter MunkAbstract:Previous studies have shown that four commercially important demersal species, namely Atlantic cod (Gadus morhua), haddock (Melanogrammus aeglefinus), whiting (Merlangius merlangus), and European Plaice (Pleuronectes platessa), spawn in distinct areas across the North Sea. Based on two comprehensive ichthyoplankton surveys in 2004 and 2009, the present study uses generalized additive mixed models to delimit these spawning grounds using the distribution of recently spawned eggs, investigates their relationship to specific environmental conditions, and examines egg dispersal during their development. Results indicate that presence–absence of early stage eggs is more related to temporal and topographic variables, while egg densities are closely linked with hydrography. Egg distribution patterns were relatively consistent during development and only changed near hatching. Compared with historic observations, the location of the spawning grounds appeared stable on the broad scale but centres of egg abundance v...
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Temporal patterns of spatial genetic structure and effective population size in European Plaice (Pleuronectes platessa) along the west coast of Scotland and in the Irish Sea
Ices Journal of Marine Science, 2009Co-Authors: Phillip C. Watts, Audrey J Geffen, Stephen J. Kemp, Suzanne Kay, Drew Wolfenden, Clive Fox, Richard D.m. NashAbstract:Watts, P. C., Kay, S. M., Wolfenden, D., Fox, C. J., Geffen, A. J., Kemp, S. J., and Nash, R. D. M. 2010. Temporal patterns of spatial genetic structure and effective population size in European Plaice (Pleuronectes platessa) along the west coast of Scotland and in the Irish Sea. - ICES Journal of Marine Science, 67: 607-616.The European Plaice (Pleuronectes platessa) is a relatively mobile flatfish species, and previous studies have reported broad-scale genetic homogeneity among samples distributed throughout much of its northern European range, with no evidence for isolation-by-distance (IBD) population structure. Using microsatellite loci, the pattern of spatial genetic structure and effective population size is characterized for >800 Plaice collected from locations off the west coast of Great Britain over a 3-year period (2001-2003). The Plaice populations are characterized by weak spatial genetic structure, consistent with tagging data, and relatively low effective population sizes. In contrast to previous work, a pattern of isolation by distance is present among pairs of Plaice from within each sampling period. However, IBD spatial structure was not observed for comparisons of Plaice from different sampling years or using the entire dataset, indicating a patchy temporal genetic structure. Therefore, pooling the data from several years can mask subtle patterns of population structure and potentially confound estimation of other important demographic parameters, such as effective population size.
Bart Ampe - One of the best experts on this subject based on the ideXlab platform.
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Inter-rater reliability of categorical versus continuous scoring of fish vitality: Does it affect the utility of the reflex action mortality predictor (RAMP) approach?
PLOS ONE, 2017Co-Authors: Pieter Meeremans, Noëlle Yochum, Marc Kochzius, Bart Ampe, Frank Tuyttens, Sven Sebastian UhlmannAbstract:Scoring reflex responsiveness and injury of aquatic organisms has gained popularity as predictors of discard survival. Given this method relies upon the individual interpretation of scoring criteria, an evaluation of its robustness is done here to test whether protocol-instructed, multiple raters with diverse backgrounds (research scientist, technician, and student) are able to produce similar or the same reflex and injury score for one of the same flatfish (European Plaice, Pleuronectes platessa) after experiencing commercial fishing stressors. Inter-rater reliability for three raters was assessed by using a 3-point categorical scale ('absent', 'weak', 'strong') and a tagged visual analogue continuous scale (tVAS, a 10 cm bar split in three labelled sections: 0 for 'absent', 'weak', 'moderate', and 'strong') for six reflex responses, and a 4-point scale for four injury types. Plaice (n = 304) were sampled from 17 research beam-trawl deployments during four trips. Fleiss kappa (categorical scores) and intra-class correlation coefficients (ICC, continuous scores) indicated variable inter-rater agreement by reflex type (ranging between 0.55 and 0.88, and 67% and 91% for Fleiss kappa and ICC, respectively), with least agreement among raters on extent of injury (Fleiss kappa between 0.08 and 0.27). Despite differences among raters, which did not significantly influence the relationship between impairment and predicted survival, combining categorical reflex and injury scores always produced a close relationship of such vitality indices and observed delayed mortality. The use of the continuous scale did not improve fit of these models compared with using the reflex impairment index based on categorical scores. Given these findings, we recommend using a 3-point categorical over a continuous scale. We also determined that training rather than experience of raters minimised inter-rater differences. Our results suggest that cost-efficient reflex impairment and injury scoring may be considered a robust technique to evaluate lethal stress and damage of this flatfish species on-board commercial beam-trawl vessels.
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Each rater’s most parsimonious logistic regression model for survival of European Plaice (Pleuronectes platessa), with co-variates: Reflex impairment and injury index based on categorical scores (R&I.cat), and total length (TL).
2017Co-Authors: Pieter Meeremans, Noëlle Yochum, Marc Kochzius, Bart Ampe, Frank A. M. Tuyttens, Sven Sebastian UhlmannAbstract:Each rater’s most parsimonious logistic regression model for survival of European Plaice (Pleuronectes platessa), with co-variates: Reflex impairment and injury index based on categorical scores (R&I.cat), and total length (TL).
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Short-term effect of pulsed direct current on various species of adult fish and its implication in pulse trawling for brown shrimp in the North Sea
Fisheries Research, 2016Co-Authors: Marieke Desender, Bart Ampe, Koen Chiers, Hans Polet, Bart Verschueren, Jimmy Saunders, Atle Mortensen, Velmurugu Puvanendran, Annemie DecostereAbstract:Abstract Electric pulses in fishing gear are increasingly used in the North Sea and are considered a promising alternative to ameliorate the sustainability of demersal trawl fisheries. The electrotrawl for brown shrimp employing low frequency pulsed direct current (PDC) selectively induces a startle response in shrimp engendering decreased environmental impact and reduced by-catch. Prior to commercially introducing this fishing technique, data on its impact on marine organisms are crucial. The aim of this study was to evaluate the short-term effects of this pulse used for electrotrawling for brown shrimp on five marine fish species inhabiting shrimp fishery areas. For this purpose, 25 European Plaice ( Pleuronectes platessa ), 30 Dover sole ( Solea solea ), 20 Atlantic cod ( Gadus morhua ), 19 bull-rout ( Myoxocephalus scorpius ) and 20 armed bullhead ( Agonus cataphractus ) were exposed to the shrimp pulse for 5 s. Before, during and till 20 min following exposure, the behaviour of the fish was monitored. Twenty-four hours post-exposure, all fish were sacrificed, inspected and samples for histological analysis were taken from the gills, dorsal muscle and internal organs. To investigate possible spinal injuries radiographs were taken. Behavioural responses were variable and species dependant. Roundfish species, cod in particular, were displaying more active and agitated fast swimming activity during exposure. The majority of flatfish showed only minor reactions and remained close to the bottom throughout the observation period. However, 15% of the exposed sole actively swam upwards during exposure. Mild multifocal petechial haemorrhages and suffusion, encountered mainly in Plaice and sole, were not significantly different between exposed and control groups. Upon histological examination, in two exposed Plaice, a focal small haemorrhage between muscle fibres was found, which was not encountered in control animals. In addition, the number of melanomacrophage centres in the spleen of exposed cod was significantly higher than in the non-exposed animals. In conclusion, under the circumstances as adopted in this study, the electrical field seemed to have only limited immediate impact on the exposed animals.
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Injury, reflex impairment, and survival of beam-trawled flatfish
Ices Journal of Marine Science, 2016Co-Authors: Sebastian S. Uhlmann, Bart Ampe, Marieke Desender, Ruben Theunynck, Maarten Soetaert, J. DepesteleAbstract:Abstract Under the “high survival” exemption of the European landing obligation or discard ban, monitoring vitality and survival of European flatfish becomes relevant to a discard-intensive beam trawl fishery. The reflex action mortality predictor (RAMP) method may be useful in this context. It involves scoring for the presence or absence of natural animal reflexes to generate an impairment score which is then correlated with post-release or discard mortality. In our first experiment, we determined suitable candidate reflexes for acclimated, laboratory-held European Plaice (Pleuronectes platessa) and common sole (Solea solea). In a second experiment, we quantified reflex impairment of commercially trawled-and-handled Plaice and sole in response to commercial fishing stressors. In a third experiment, we tested whether a combined reflex impairment and injury (vitality) score of Plaice was correlated with delayed post-release mortality to establish RAMP. Five-hundred fourteen trawled-and-discarded Plaice and 176 sole were assessed for experimentally confirmed reflexes such as righting, evasion, stabilise, and tail grab, among others. Of these fish, 316 Plaice were monitored for at least 14 d in captivity, alongside 60 control Plaice. All control fish survived, together with an average of 50% (±29 SD) Plaice after being trawled from conventional, 60 min trawls and sorted on-board a coastal beam trawler. Stressors such as trawl duration, wave height, air, and seawater temperature were not as relevant as a vitality score and total length in predicting post-release survival probability. In the second experiment where survival was not assessed, reflex impairment of Plaice became more frequent with prolonged air exposure. For sole, a researcher handling-and-reflex scoring bias rather than a fishing stressor may have confounded results. Scoring a larger number of individuals for injuries and reflexes from a representative selection of trawls and trips may allow for a fleet-scale discard survival estimate to facilitate implementation of the discard ban.
Rosario Martín - One of the best experts on this subject based on the ideXlab platform.
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identification of flatfish species using polymerase chain reaction pcr amplification and restriction analysis of the cytochrome b gene
Journal of Food Science, 2008Co-Authors: Ana Céspedes, Teresa García, Esther Carrera, Isabel González, Pablo E. Hernández, Bernabé Sanz, Rosario MartínAbstract:ANA CESPEDES, TERESA GARCIA, ESTHER CARRERA, ISABEL GONZALEZ,BERNABE SANZ, PABLO E. HERNANDEZ and ROSARIO MARTINABSTRACTRestriction site analysis of PCR products from a conservedregion of the cytochrome b gene has been used for the spe-cific identification of sole (Solea solea), European Plaice(Pleuronectes platessa) and flounder (Platichthys flesus).Polymerase chain reaction (PCR) amplification of the cyto-chrome b gene using universal primers produced a 359 bpfragment in all species analyzed. Digestion of the PCR prod-ucts with Nci I, Sau 3AI and Hinf I endonucleases, followed byagarose gel electrophoresis of the digested PCR products,yielded specific profiles that enabled direct identification ofthe fish species. This methodology should prove useful forenforcing labeling regulations in the authentication of flatfishspecies.Key Words: species, identification, flatfish, PCR, cyto-chrome b
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Indirect enzyme-linked immunosorbent assay for the identification of sole (Solea solea), European Plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides).
Journal of Food Protection, 1999Co-Authors: Ana Céspedes, Teresa García, Esther Carrera, Isabel González, Alicia Fernández, Luis Asensio, Pablo E. Hernández, Rosario MartínAbstract:Polyclonal antibodies produced against soluble muscle protein extracts from sole (Solea solea), European Plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides) were used in an indirect enzyme-linked immunosorbent assay for the specific identification of fillets from these flatfish species. The assay was performed in two different formats: microtiter plates and immunostick tubes. Immunorecognition of antibodies adsorbed to their specific fish samples was made with goat antirabbit immunoglobulins conjugated to the enzyme horseradish peroxidase. Subsequent enzymatic conversion of the substrate allowed unequivocal identification of all flatfish species studied.
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Application of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) to identification of flatfish species.
Journal of AOAC International, 1999Co-Authors: Ana Céspedes, Teresa García, Esther Carrera, Isabel González, Alicia Fernández, Pablo E. Hernández, Rosario MartínAbstract:A method of DNA analysis based on polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) was developed to verify the authenticity of labeled raw and frozen fillets of some flatfish species. PCR was used to amplify a short fragment (201 bp) of the mitochondrial cytochrome b gene, which was denatured and analyzed by native polyacrylamide gel electrophoresis for detection of SSCPs. Species-specific patterns of DNA bands were obtained for sole (Solea solea), European Plaice (Pleuronectes platessa), flounder (Platlchthys flesus), and Greenland halibut (Reinhardtius hippoglossoldes).
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Polymerase chain reaction-restriction fragment length polymorphism analysis of a short fragment of the cytochrome b gene for identification of flatfish species.
Journal of Food Protection, 1998Co-Authors: Ana Céspedes, Teresa García, Esther Carrera, Isabel González, Pablo E. Hernández, Bernabé Sanz, Rosario MartínAbstract:Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the cytochrome b gene has been used for the specific identification of sole (Solea solea), European Plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides). PCR amplification of the cytochrome b gene using a universal primer together with a primer specifically designed as a part of this study produced a 201-bp fragment in all species analyzed. Digestions of the PCR products with Sau3Al, BsmAl, Rsal, and Mn/l endonucleases, followed by agarose gel electrophoresis of the digested PCR products, yielded specific profiles that enabled direct identification of each species analyzed.
