The Experts below are selected from a list of 69 Experts worldwide ranked by ideXlab platform
J F Pays - One of the best experts on this subject based on the ideXlab platform.
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The painstaking discovery of the hidden face of the human plasmodia
Parasitology Research, 2012Co-Authors: J F PaysAbstract:The difficulties encountered in the discovery of the Exoerythrocytic Stage of asexual cycle of human plasmodia are described. These illustrate how deference towards scientific orthodoxy and a degree of reluctance to question and to criticise can delay advances in knowledge.
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Cycle exoérythrocytaire asexué des plasmodiums humains : une découverte laborieuse
Bulletin de la Société de pathologie exotique, 2010Co-Authors: J F PaysAbstract:L’auteur relate l’histoire de la découverte laborieuse du cycle exoérythrocytaire asexué des plasmodiums inféodés à l’homme en rappelant combien la déférence vis-à-vis de l’ establishment scientifique du moment et une certaine timidité dans la contestation et la critique peuvent être préjudiciables aux avancées de la connaissance. The difficulties encountered in the discovery of the Exoerythrocytic Stage of the asexual cycle of the human plasmodia are described. These illustrate how deference towards scientific orthodoxy and a degree of reluctance to question and to criticise can delay advances in knowledge.
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characterisation of the Exoerythrocytic Stage of the asexual cycle of the human plasmodia a painstaking process
Bulletin De La Societe De Pathologie Exotique, 2010Co-Authors: J F PaysAbstract:: The difficulties encountered in the discovery of the Exoerythrocytic Stage of the asexual cycle of the human plasmodia are described. These illustrate how deference towards scientific orthodoxy and a degree of reluctance to question and to criticize can delay advances in knowledge.
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Cycle exoérythrocytaire asexué des plasmodiums humains : une découverte laborieuse@@@Characterisation of the Exoerythrocytic Stage of the asexual cycle of the human plasmodia: a painstaking process
Bulletin De La Societe De Pathologie Exotique, 2010Co-Authors: J F PaysAbstract:: The difficulties encountered in the discovery of the Exoerythrocytic Stage of the asexual cycle of the human plasmodia are described. These illustrate how deference towards scientific orthodoxy and a degree of reluctance to question and to criticize can delay advances in knowledge.
Justine Swann - One of the best experts on this subject based on the ideXlab platform.
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Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium Exoerythrocytic infection.
Nature Communications, 2019Co-Authors: Gregory M. Lamonte, Justine Swann, Pamela Orjuela-sanchez, Jaeson Calla, Lawrence T. Wang, Shangzhong Li, Annie N. Cowell, Alyaa Mohamed, Zaira Hellen Villa GalarceAbstract:The Exoerythrocytic Stage of Plasmodium infection is a critical window for prophylactic intervention. Using genome-wide dual rna-sequencing/">RNA sequencing of flow-sorted infected and uninfected hepatoma cells we show that the human mucosal immunity gene, mucin-13 (MUC13), is strongly upregulated during Plasmodium Exoerythrocytic hepatic-Stage infection. We confirm MUC13 transcript increases in hepatoma cell lines and primary hepatocytes. In immunofluorescence assays, host MUC13 protein expression distinguishes infected cells from adjacent uninfected cells and shows similar colocalization with parasite biomarkers such as UIS4 and HSP70. We further show that localization patterns are species independent, marking both P. berghei and P. vivax infected cells, and that MUC13 can be used to identify compounds that inhibit parasite replication in hepatocytes. This data provides insights into host-parasite interactions in Plasmodium infection, and demonstrates that a component of host mucosal immunity is reprogrammed during the progression of infection.
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Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium Exoerythrocytic infection
Nature Communications, 2019Co-Authors: Gregory M. Lamonte, Justine Swann, Pamela Orjuela-sanchez, Jaeson Calla, Lawrence T. Wang, Shangzhong Li, Annie N. Cowell, Alyaa M. Abdel-haleem Mohamed, Zaira Hellen Villa Galarce, Marta MorenoAbstract:Host-parasite interactions during the Exoerythrocytic Stage of Plasmodium infection remains poorly understood. Using dual RNA-Seq, the authors show that human mucosal immunity protein mucin-13 is upregulated during Plasmodium hepatic-Stage infection and marks infected cells independent of tested Plasmodium species. The Exoerythrocytic Stage of Plasmodium infection is a critical window for prophylactic intervention. Using genome-wide dual rna-sequencing/">RNA sequencing of flow-sorted infected and uninfected hepatoma cells we show that the human mucosal immunity gene, mucin-13 (MUC13), is strongly upregulated during Plasmodium Exoerythrocytic hepatic-Stage infection. We confirm MUC13 transcript increases in hepatoma cell lines and primary hepatocytes. In immunofluorescence assays, host MUC13 protein expression distinguishes infected cells from adjacent uninfected cells and shows similar colocalization with parasite biomarkers such as UIS4 and HSP70. We further show that localization patterns are species independent, marking both P. berghei and P. vivax infected cells, and that MUC13 can be used to identify compounds that inhibit parasite replication in hepatocytes. This data provides insights into host-parasite interactions in Plasmodium infection, and demonstrates that a component of host mucosal immunity is reprogrammed during the progression of infection.
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high throughput luciferase based assay for the discovery of therapeutics that prevent malaria
ACS Infectious Diseases, 2016Co-Authors: Justine Swann, Victoria C Corey, Christina Scherer, Nobutaka Kato, Eamon Comer, Micah Maetani, Yevgeniya Antonovakoch, Christin Reimer, Kerstin Gagaring, Maureen IbanezAbstract:In order to identify the most attractive starting points for drugs that can be used to prevent malaria, a diverse chemical space comprising tens of thousands to millions of small molecules may need to be examined. Achieving this throughput necessitates the development of efficient ultra-high-throughput screening methods. Here, we report the development and evaluation of a luciferase-based phenotypic screen of malaria Exoerythrocytic-Stage parasites optimized for a 1536-well format. This assay uses the Exoerythrocytic Stage of the rodent malaria parasite, Plasmodium berghei, and a human hepatoma cell line. We use this assay to evaluate several biased and unbiased compound libraries, including two small sets of molecules (400 and 89 compounds, respectively) with known activity against malaria erythrocytic-Stage parasites and a set of 9886 diversity-oriented synthesis (DOS)-derived compounds. Of the compounds screened, we obtain hit rates of 12–13 and 0.6% in preselected and naive libraries, respectively, an...
Marta Moreno - One of the best experts on this subject based on the ideXlab platform.
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Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium Exoerythrocytic infection
Nature Communications, 2019Co-Authors: Gregory M. Lamonte, Justine Swann, Pamela Orjuela-sanchez, Jaeson Calla, Lawrence T. Wang, Shangzhong Li, Annie N. Cowell, Alyaa M. Abdel-haleem Mohamed, Zaira Hellen Villa Galarce, Marta MorenoAbstract:Host-parasite interactions during the Exoerythrocytic Stage of Plasmodium infection remains poorly understood. Using dual RNA-Seq, the authors show that human mucosal immunity protein mucin-13 is upregulated during Plasmodium hepatic-Stage infection and marks infected cells independent of tested Plasmodium species. The Exoerythrocytic Stage of Plasmodium infection is a critical window for prophylactic intervention. Using genome-wide dual rna-sequencing/">RNA sequencing of flow-sorted infected and uninfected hepatoma cells we show that the human mucosal immunity gene, mucin-13 (MUC13), is strongly upregulated during Plasmodium Exoerythrocytic hepatic-Stage infection. We confirm MUC13 transcript increases in hepatoma cell lines and primary hepatocytes. In immunofluorescence assays, host MUC13 protein expression distinguishes infected cells from adjacent uninfected cells and shows similar colocalization with parasite biomarkers such as UIS4 and HSP70. We further show that localization patterns are species independent, marking both P. berghei and P. vivax infected cells, and that MUC13 can be used to identify compounds that inhibit parasite replication in hepatocytes. This data provides insights into host-parasite interactions in Plasmodium infection, and demonstrates that a component of host mucosal immunity is reprogrammed during the progression of infection.
William E Collins - One of the best experts on this subject based on the ideXlab platform.
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Plasmodium ovale : observations on the parasite development in Saimiri monkey hepatocytes in vivo and in vitro in contrast with its inability to induce parasitemia
Experimental Parasitology, 1994Co-Authors: Pascal Millet, C. Nelson, G. Gale Galland, Joann S. Sullivan, Carla L. Morris, Bettye B. Richardson, William E CollinsAbstract:Abstract Exoerythrocytic Stage parasites of the human malaria parasite Plasmodium ovale were cultured in vitro by inoculating primary cultures of hepatocytes from Saimiri sciureus boliviensis monkeys with sporozoites. Morphology and size of the liver Stages were similar to previous in vivo descriptions in humans and chimpanzees. Saimiri monkeys did not develop parasitemia after repeated inoculations with P. ovale sporozoites. However, liver-Stage parasites were observed in liver biopsies performed 7 days after sporozoite inoculation. Together with observations on other parasite development, these results demonstrate that host specificity for many malaria parasites occurs at the blood-Stage level. Lack of host specificity of primary malaria parasite species for the liver forms the basis for the close relationship existing between human and nonhuman primate malaria species.
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In vitro cultivation of Exoerythrocytic Stages of the simian malaria parasites Plasmodium fieldi and Plasmodium simiovale in rhesus monkey hepatocytes.
Journal of Parasitology, 1994Co-Authors: Pascal Millet, Patrick Anderson, William E CollinsAbstract:Exoerythrocytic Stage parasites of Plasmodium fieldi and Plasmodium simiovale, 2 simian malaria parasites related to the human malaria parasite Plasmodium ovale, were cultured in vitro by inoculating primary cultures of hepatocytes from rhesus monkeys (Macaca mulatta) with sporozoites. Less than 1% of sporozoites developed into schizonts for either species. Structure and size of the liver Stages in both species were similar to previous in vivo descriptions, and the time required for in vitro maturation correlated well with the prepatent periods described for each species. Such monkey models could be very useful in conducting scientific investi- gations on the pre-erythrocytic Stages of P. ovale-like malaria parasites. In the life cycle of malaria parasites, exoery- throcytic (EE) Stages constitute an essential link between the sporozoites inoculated by a mos- quito bite and the blood Stages responsible for symptoms of the disease. However, investiga- tions on EE Stages are hampered by their short duration and by the fact that in mammalian ma- laria parasites, these Stages occur in a deep organ,
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in vitro maturation of the Exoerythrocytic Stage of plasmodium knowlesi observed under phase contrast microscopy
Journal of Parasitology, 1990Co-Authors: Pascal Millet, William E Collins, Claude E Monken, Bobby G BrownAbstract:: Exoerythrocytic Stages of Plasmodium knowlesi were obtained in primary culture of rhesus monkey hepatocytes. The development of a single parasite was followed with phase contrast microscopy until release of merozoites in slightly less than 5 days. This direct observation may offer opportunities to determine visually factors that may influence specific steps of schizont development.
Gregory M. Lamonte - One of the best experts on this subject based on the ideXlab platform.
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Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium Exoerythrocytic infection
Nature Communications, 2019Co-Authors: Gregory M. Lamonte, Justine Swann, Pamela Orjuela-sanchez, Jaeson Calla, Lawrence T. Wang, Shangzhong Li, Annie N. Cowell, Alyaa M. Abdel-haleem Mohamed, Zaira Hellen Villa Galarce, Marta MorenoAbstract:Host-parasite interactions during the Exoerythrocytic Stage of Plasmodium infection remains poorly understood. Using dual RNA-Seq, the authors show that human mucosal immunity protein mucin-13 is upregulated during Plasmodium hepatic-Stage infection and marks infected cells independent of tested Plasmodium species. The Exoerythrocytic Stage of Plasmodium infection is a critical window for prophylactic intervention. Using genome-wide dual rna-sequencing/">RNA sequencing of flow-sorted infected and uninfected hepatoma cells we show that the human mucosal immunity gene, mucin-13 (MUC13), is strongly upregulated during Plasmodium Exoerythrocytic hepatic-Stage infection. We confirm MUC13 transcript increases in hepatoma cell lines and primary hepatocytes. In immunofluorescence assays, host MUC13 protein expression distinguishes infected cells from adjacent uninfected cells and shows similar colocalization with parasite biomarkers such as UIS4 and HSP70. We further show that localization patterns are species independent, marking both P. berghei and P. vivax infected cells, and that MUC13 can be used to identify compounds that inhibit parasite replication in hepatocytes. This data provides insights into host-parasite interactions in Plasmodium infection, and demonstrates that a component of host mucosal immunity is reprogrammed during the progression of infection.
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Dual RNA-seq identifies human mucosal immunity protein Mucin-13 as a hallmark of Plasmodium Exoerythrocytic infection.
Nature Communications, 2019Co-Authors: Gregory M. Lamonte, Justine Swann, Pamela Orjuela-sanchez, Jaeson Calla, Lawrence T. Wang, Shangzhong Li, Annie N. Cowell, Alyaa Mohamed, Zaira Hellen Villa GalarceAbstract:The Exoerythrocytic Stage of Plasmodium infection is a critical window for prophylactic intervention. Using genome-wide dual rna-sequencing/">RNA sequencing of flow-sorted infected and uninfected hepatoma cells we show that the human mucosal immunity gene, mucin-13 (MUC13), is strongly upregulated during Plasmodium Exoerythrocytic hepatic-Stage infection. We confirm MUC13 transcript increases in hepatoma cell lines and primary hepatocytes. In immunofluorescence assays, host MUC13 protein expression distinguishes infected cells from adjacent uninfected cells and shows similar colocalization with parasite biomarkers such as UIS4 and HSP70. We further show that localization patterns are species independent, marking both P. berghei and P. vivax infected cells, and that MUC13 can be used to identify compounds that inhibit parasite replication in hepatocytes. This data provides insights into host-parasite interactions in Plasmodium infection, and demonstrates that a component of host mucosal immunity is reprogrammed during the progression of infection.