The Experts below are selected from a list of 10584 Experts worldwide ranked by ideXlab platform
Germain Trugnan - One of the best experts on this subject based on the ideXlab platform.
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Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo. Use of a "lectin assay" to survey in vivo glycosylation changes.
Histochemistry and cell biology, 2005Co-Authors: Miguel Freitas, Lars-göran Axelsson, Chantal Cayuela, Tore Midtvedt, Germain TrugnanAbstract:It has been shown that Bacteroides thetaiotaomicron, a representative member of the gut microflora, signals intestinal epithelial cells both in vivo and in vitro and modulate specific glycosylation processes that may mediate intestinal functions. However it is not known whether these modulations depend on the presence of live Bacteria or may be elicited by soluble factors produced in vitro by this bacterium. We used lectins and an histochemical approach to survey tissue sections prepared from various cellular compartments of the small and large intestine of NRMI/KI mice grown under gnotobiotic conditions. We compared the results obtained with Bacterial culture supernatant and live B. thetaiotaomicron to those obtained from germ-free mice or mice having a conventional microflora. This approach allowed us to conclude that (1) a small but specific number of glycan patterns were restored after treatment with Bacterial culture supernatant and (2) the B. thetaiotaomicron associated mice restored a larger number of patterns, however, the complete conventional mice pattern must be a function of the whole microflora in the gut. The possibility to modulate this complex glycosylation pattern by introducing Exogenous Bacteria and Bacterial products should be considered as a promising approach towards understanding the molecular basis of microbial-host interactions.
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Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo
Histochemistry and Cell Biology, 2005Co-Authors: Miguel Freitas, Lars-göran Axelsson, Chantal Cayuela, Tore Midtvedt, Germain TrugnanAbstract:It has been shown that Bacteroides thetaiotaomicron , a representative member of the gut microflora, signals intestinal epithelial cells both in vivo and in vitro and modulate specific glycosylation processes that may mediate intestinal functions. However it is not known whether these modulations depend on the presence of live Bacteria or may be elicited by soluble factors produced in vitro by this bacterium. We used lectins and an histochemical approach to survey tissue sections prepared from various cellular compartments of the small and large intestine of NRMI/KI mice grown under gnotobiotic conditions. We compared the results obtained with Bacterial culture supernatant and live B. thetaiotaomicron to those obtained from germ-free mice or mice having a conventional microflora. This approach allowed us to conclude that (1) a small but specific number of glycan patterns were restored after treatment with Bacterial culture supernatant and (2) the B. thetaiotaomicron associated mice restored a larger number of patterns, however, the complete conventional mice pattern must be a function of the whole microflora in the gut. The possibility to modulate this complex glycosylation pattern by introducing Exogenous Bacteria and Bacterial products should be considered as a promising approach towards understanding the molecular basis of microbial-host interactions.
Miguel Freitas - One of the best experts on this subject based on the ideXlab platform.
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Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo. Use of a "lectin assay" to survey in vivo glycosylation changes.
Histochemistry and cell biology, 2005Co-Authors: Miguel Freitas, Lars-göran Axelsson, Chantal Cayuela, Tore Midtvedt, Germain TrugnanAbstract:It has been shown that Bacteroides thetaiotaomicron, a representative member of the gut microflora, signals intestinal epithelial cells both in vivo and in vitro and modulate specific glycosylation processes that may mediate intestinal functions. However it is not known whether these modulations depend on the presence of live Bacteria or may be elicited by soluble factors produced in vitro by this bacterium. We used lectins and an histochemical approach to survey tissue sections prepared from various cellular compartments of the small and large intestine of NRMI/KI mice grown under gnotobiotic conditions. We compared the results obtained with Bacterial culture supernatant and live B. thetaiotaomicron to those obtained from germ-free mice or mice having a conventional microflora. This approach allowed us to conclude that (1) a small but specific number of glycan patterns were restored after treatment with Bacterial culture supernatant and (2) the B. thetaiotaomicron associated mice restored a larger number of patterns, however, the complete conventional mice pattern must be a function of the whole microflora in the gut. The possibility to modulate this complex glycosylation pattern by introducing Exogenous Bacteria and Bacterial products should be considered as a promising approach towards understanding the molecular basis of microbial-host interactions.
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Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo
Histochemistry and Cell Biology, 2005Co-Authors: Miguel Freitas, Lars-göran Axelsson, Chantal Cayuela, Tore Midtvedt, Germain TrugnanAbstract:It has been shown that Bacteroides thetaiotaomicron , a representative member of the gut microflora, signals intestinal epithelial cells both in vivo and in vitro and modulate specific glycosylation processes that may mediate intestinal functions. However it is not known whether these modulations depend on the presence of live Bacteria or may be elicited by soluble factors produced in vitro by this bacterium. We used lectins and an histochemical approach to survey tissue sections prepared from various cellular compartments of the small and large intestine of NRMI/KI mice grown under gnotobiotic conditions. We compared the results obtained with Bacterial culture supernatant and live B. thetaiotaomicron to those obtained from germ-free mice or mice having a conventional microflora. This approach allowed us to conclude that (1) a small but specific number of glycan patterns were restored after treatment with Bacterial culture supernatant and (2) the B. thetaiotaomicron associated mice restored a larger number of patterns, however, the complete conventional mice pattern must be a function of the whole microflora in the gut. The possibility to modulate this complex glycosylation pattern by introducing Exogenous Bacteria and Bacterial products should be considered as a promising approach towards understanding the molecular basis of microbial-host interactions.
Chantal Cayuela - One of the best experts on this subject based on the ideXlab platform.
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Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo. Use of a "lectin assay" to survey in vivo glycosylation changes.
Histochemistry and cell biology, 2005Co-Authors: Miguel Freitas, Lars-göran Axelsson, Chantal Cayuela, Tore Midtvedt, Germain TrugnanAbstract:It has been shown that Bacteroides thetaiotaomicron, a representative member of the gut microflora, signals intestinal epithelial cells both in vivo and in vitro and modulate specific glycosylation processes that may mediate intestinal functions. However it is not known whether these modulations depend on the presence of live Bacteria or may be elicited by soluble factors produced in vitro by this bacterium. We used lectins and an histochemical approach to survey tissue sections prepared from various cellular compartments of the small and large intestine of NRMI/KI mice grown under gnotobiotic conditions. We compared the results obtained with Bacterial culture supernatant and live B. thetaiotaomicron to those obtained from germ-free mice or mice having a conventional microflora. This approach allowed us to conclude that (1) a small but specific number of glycan patterns were restored after treatment with Bacterial culture supernatant and (2) the B. thetaiotaomicron associated mice restored a larger number of patterns, however, the complete conventional mice pattern must be a function of the whole microflora in the gut. The possibility to modulate this complex glycosylation pattern by introducing Exogenous Bacteria and Bacterial products should be considered as a promising approach towards understanding the molecular basis of microbial-host interactions.
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Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo
Histochemistry and Cell Biology, 2005Co-Authors: Miguel Freitas, Lars-göran Axelsson, Chantal Cayuela, Tore Midtvedt, Germain TrugnanAbstract:It has been shown that Bacteroides thetaiotaomicron , a representative member of the gut microflora, signals intestinal epithelial cells both in vivo and in vitro and modulate specific glycosylation processes that may mediate intestinal functions. However it is not known whether these modulations depend on the presence of live Bacteria or may be elicited by soluble factors produced in vitro by this bacterium. We used lectins and an histochemical approach to survey tissue sections prepared from various cellular compartments of the small and large intestine of NRMI/KI mice grown under gnotobiotic conditions. We compared the results obtained with Bacterial culture supernatant and live B. thetaiotaomicron to those obtained from germ-free mice or mice having a conventional microflora. This approach allowed us to conclude that (1) a small but specific number of glycan patterns were restored after treatment with Bacterial culture supernatant and (2) the B. thetaiotaomicron associated mice restored a larger number of patterns, however, the complete conventional mice pattern must be a function of the whole microflora in the gut. The possibility to modulate this complex glycosylation pattern by introducing Exogenous Bacteria and Bacterial products should be considered as a promising approach towards understanding the molecular basis of microbial-host interactions.
Tore Midtvedt - One of the best experts on this subject based on the ideXlab platform.
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Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo. Use of a "lectin assay" to survey in vivo glycosylation changes.
Histochemistry and cell biology, 2005Co-Authors: Miguel Freitas, Lars-göran Axelsson, Chantal Cayuela, Tore Midtvedt, Germain TrugnanAbstract:It has been shown that Bacteroides thetaiotaomicron, a representative member of the gut microflora, signals intestinal epithelial cells both in vivo and in vitro and modulate specific glycosylation processes that may mediate intestinal functions. However it is not known whether these modulations depend on the presence of live Bacteria or may be elicited by soluble factors produced in vitro by this bacterium. We used lectins and an histochemical approach to survey tissue sections prepared from various cellular compartments of the small and large intestine of NRMI/KI mice grown under gnotobiotic conditions. We compared the results obtained with Bacterial culture supernatant and live B. thetaiotaomicron to those obtained from germ-free mice or mice having a conventional microflora. This approach allowed us to conclude that (1) a small but specific number of glycan patterns were restored after treatment with Bacterial culture supernatant and (2) the B. thetaiotaomicron associated mice restored a larger number of patterns, however, the complete conventional mice pattern must be a function of the whole microflora in the gut. The possibility to modulate this complex glycosylation pattern by introducing Exogenous Bacteria and Bacterial products should be considered as a promising approach towards understanding the molecular basis of microbial-host interactions.
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Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo
Histochemistry and Cell Biology, 2005Co-Authors: Miguel Freitas, Lars-göran Axelsson, Chantal Cayuela, Tore Midtvedt, Germain TrugnanAbstract:It has been shown that Bacteroides thetaiotaomicron , a representative member of the gut microflora, signals intestinal epithelial cells both in vivo and in vitro and modulate specific glycosylation processes that may mediate intestinal functions. However it is not known whether these modulations depend on the presence of live Bacteria or may be elicited by soluble factors produced in vitro by this bacterium. We used lectins and an histochemical approach to survey tissue sections prepared from various cellular compartments of the small and large intestine of NRMI/KI mice grown under gnotobiotic conditions. We compared the results obtained with Bacterial culture supernatant and live B. thetaiotaomicron to those obtained from germ-free mice or mice having a conventional microflora. This approach allowed us to conclude that (1) a small but specific number of glycan patterns were restored after treatment with Bacterial culture supernatant and (2) the B. thetaiotaomicron associated mice restored a larger number of patterns, however, the complete conventional mice pattern must be a function of the whole microflora in the gut. The possibility to modulate this complex glycosylation pattern by introducing Exogenous Bacteria and Bacterial products should be considered as a promising approach towards understanding the molecular basis of microbial-host interactions.
Lars-göran Axelsson - One of the best experts on this subject based on the ideXlab platform.
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Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo. Use of a "lectin assay" to survey in vivo glycosylation changes.
Histochemistry and cell biology, 2005Co-Authors: Miguel Freitas, Lars-göran Axelsson, Chantal Cayuela, Tore Midtvedt, Germain TrugnanAbstract:It has been shown that Bacteroides thetaiotaomicron, a representative member of the gut microflora, signals intestinal epithelial cells both in vivo and in vitro and modulate specific glycosylation processes that may mediate intestinal functions. However it is not known whether these modulations depend on the presence of live Bacteria or may be elicited by soluble factors produced in vitro by this bacterium. We used lectins and an histochemical approach to survey tissue sections prepared from various cellular compartments of the small and large intestine of NRMI/KI mice grown under gnotobiotic conditions. We compared the results obtained with Bacterial culture supernatant and live B. thetaiotaomicron to those obtained from germ-free mice or mice having a conventional microflora. This approach allowed us to conclude that (1) a small but specific number of glycan patterns were restored after treatment with Bacterial culture supernatant and (2) the B. thetaiotaomicron associated mice restored a larger number of patterns, however, the complete conventional mice pattern must be a function of the whole microflora in the gut. The possibility to modulate this complex glycosylation pattern by introducing Exogenous Bacteria and Bacterial products should be considered as a promising approach towards understanding the molecular basis of microbial-host interactions.
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Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo
Histochemistry and Cell Biology, 2005Co-Authors: Miguel Freitas, Lars-göran Axelsson, Chantal Cayuela, Tore Midtvedt, Germain TrugnanAbstract:It has been shown that Bacteroides thetaiotaomicron , a representative member of the gut microflora, signals intestinal epithelial cells both in vivo and in vitro and modulate specific glycosylation processes that may mediate intestinal functions. However it is not known whether these modulations depend on the presence of live Bacteria or may be elicited by soluble factors produced in vitro by this bacterium. We used lectins and an histochemical approach to survey tissue sections prepared from various cellular compartments of the small and large intestine of NRMI/KI mice grown under gnotobiotic conditions. We compared the results obtained with Bacterial culture supernatant and live B. thetaiotaomicron to those obtained from germ-free mice or mice having a conventional microflora. This approach allowed us to conclude that (1) a small but specific number of glycan patterns were restored after treatment with Bacterial culture supernatant and (2) the B. thetaiotaomicron associated mice restored a larger number of patterns, however, the complete conventional mice pattern must be a function of the whole microflora in the gut. The possibility to modulate this complex glycosylation pattern by introducing Exogenous Bacteria and Bacterial products should be considered as a promising approach towards understanding the molecular basis of microbial-host interactions.