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Edison Osorio - One of the best experts on this subject based on the ideXlab platform.
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characterization of polyphenol oxidase from cape gooseberry physalis peruviana l Fruit
Food Chemistry, 2016Co-Authors: Karent Bravo, Edison OsorioAbstract:Abstract Cape gooseberry ( Physalis peruviana ) is an Exotic Fruit highly valued, however it is a very rich source of polyphenol oxidase (PPO). In this study, Cape gooseberry PPO was isolated and biochemically characterized. The enzyme was extracted and purified using acetone and aqueous two-phase systems. The data indicated that PPO had the highest substrate affinity for chlorogenic acid, 4-methylcatechol and catechol. Chlorogenic acid was the most suitable substrate ( K m = 0.56 ± 0.07 mM and V max = 53.15 ± 2.03 U PPO mL −1 min −1 ). The optimal pH values were 5.5 for catechol and 4-methylcatechol and 5.0 for chlorogenic acid. Optimal temperatures were 40 °C for catechol, 25 °C for 4-methylcatechol and 20 °C for chlorogenic acid. In inhibition tests, the most potent inhibitor was found to be ascorbic acid followed by l -cysteine and quercetin. This study shows possible treatments that can be implemented during the processing of Cape gooseberry Fruits to prevent browning.
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influence of cultivar and ripening time on bioactive compounds and antioxidant properties in cape gooseberry physalis peruviana l
Journal of the Science of Food and Agriculture, 2015Co-Authors: Karent Bravo, Stella Sepulvedaortega, Oscar J Laraguzman, Alejandro A Navasarboleda, Edison OsorioAbstract:BACKGROUND Cape gooseberry (Physalis peruviana) is an Exotic Fruit highly valued for its organoleptic properties and bioactive compounds. Considering that the presence of phenolics and ascorbic acid could contribute to its functional capacity, it is important to investigate the quality parameters, bioactive contents and functional properties with respect to genotype and ripening time. In this study the genotype effect was evaluated in 15 cultivars for two different harvest times. Changes during maturation were recorded in two commercial cultivars within seven levels of maturity. RESULTS Multivariate statistical analysis suggested that phenolic content and ORAC value were mainly affected by harvest time and that ascorbic acid content and DPPH level were mainly affected by genotype. In addition, acidity, phenolic content, ORAC value and inhibition of LDL oxidation decreased with maturity, but soluble solids content, ascorbic acid content, β-carotene content and DPPH-scavenging activity were higher in mature Fruits. CONCLUSION The phenolic content, ascorbic acid content and antioxidant properties of Cape gooseberry Fruit were strongly affected by cultivar, harvest time and maturity state. Consequently, the harvest time must be scheduled carefully to gain the highest proportion of bioactive compounds according to the specific cultivar and the environment where it is grown. © 2014 Society of Chemical Industry
Ruales Jenny - One of the best experts on this subject based on the ideXlab platform.
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Banana passion Fruit (Passiflora mollissima (Kunth) L.H. Bailey): microencapsulation, phytochemical composition and antioxidant capacity
'MDPI AG', 2017Co-Authors: García Ruiz Rosa, Girones Vilaplana Amadeo, León Paola, Moreno, Diego A., Stinco Scanarotti, Carla Maria, Meléndez Martínez, Antonio Jesús, Ruales JennyAbstract:Passiflora mollissima (Kunth) L.H. Bailey is an Exotic Fruit native to South America, known as taxo in Ecuador. This paper characterizes its flavonoid and carotenoid composition and antioxidant capacity and evaluates the effect of the spray-drying process on its phytochemical composition and antioxidant capacity. A total of 18 flavonoid compounds, nine proanthocyanidins and nine flavan-3-ol monomers, were identified and quantified. Glycosides of (epi)-afzelechin stood out as the most abundant flavonoid. Three carotenoids were identified, with -carotene having the highest concentration. The DPPH and ORAC assay methods indicated a high antioxidant capacity. Furthermore, the bioactive content showed a positive and direct correlation with antioxidant capacity. On the other hand, the spray-drying process produced a stable phytochemical composition and antioxidant activity of taxo. These results demonstrate the potential applicability of microencapsulated taxo as a functional ingredient in the food industry.Ministerio de Economía y Competitividad (I-COOP+2014 (Ref. COOPB20125))CSIC-VITRI (I-COOP+2014 (Ref. COOPB20125))Escuela Politécnica Nacional de Ecuador (PIS 12-21) and PIMI (14-14)CYTED Program (Refs. 112RT0460-CORNUCOPIA and 112RT0445-IBERCAROT, Thematic Networks
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Banana Passion Fruit (Passiflora mollissima (Kunth) L.H. Bailey): Microencapsulation, Phytochemical Composition and Antioxidant Capacity
'MDPI AG', 2017Co-Authors: García-ruiz Almudena, Girones Vilaplana Amadeo, León Paola, Moreno, Diego A., Stinco, Carla M., Meléndez-martínez, Antonio J., Ruales JennyAbstract:Passiflora mollissima (Kunth) L.H. Bailey is an Exotic Fruit native to South America, known as taxo in Ecuador. This paper characterizes its flavonoid and carotenoid composition and antioxidant capacity and evaluates the effect of the spray-drying process on its phytochemical composition and antioxidant capacity. A total of 18 flavonoid compounds, nine proanthocyanidins and nine flavan-3-ol monomers, were identified and quantified. Glycosides of (epi)-afzelechin stood out as the most abundant flavonoid. Three carotenoids were identified, with β-carotene having the highest concentration. The DPPH· and ORAC assay methods indicated a high antioxidant capacity. Furthermore, the bioactive content showed a positive and direct correlation with antioxidant capacity. On the other hand, the spray-drying process produced a stable phytochemical composition and antioxidant activity of taxo. These results demonstrate the potential applicability of microencapsulated taxo as a functional ingredient in the food industry.We acknowledge support by the CSIC Open Access Publication Initiative through its Unit of Information Resources for Research (URICI).: Quality technical work from Ana Benítez González (Universidad de Sevilla) is acknowledged. María Torres reviewed the English edition of this study. The authors would like to thank the Spanish Ministry of Economy and Competitiveness (MINECO) and CSIC-VITRI with the Project I-COOP+2014 (Ref. COOPB20125) for funding this project. This study was financed by PIS 12-21 and PIMI 14-14 sponsored by Escuela Politécnica Nacional. Part of this work was carried out in international research collaboration within the CYTED Program (Refs. 112RT0460-CORNUCOPIA and 112RT0445-IBERCAROT, Thematic Networks). AGR also thanks the Ecuadorian Secretary of Higher Education, Science, Technology and Innovation for the Prometeo Postdoctoral Grant
Fernando A Cabral - One of the best experts on this subject based on the ideXlab platform.
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composition of supercritical carbon dioxide extracts of pitanga eugenia uniflora l leaves
Journal of Food Process Engineering, 2009Co-Authors: Camila Arantes Peixoto, Alessandra Lopes De Oliveira, Fernando A CabralAbstract:The leaves of the Pitanga bush (Eugenia uniflora L.) are considered to be effective against many diseases. Extracts from Pitanga leaves have been found to show pronounced anti-inflammatory action and to have antimicrobial and antifungal activities, among other properties. In this work, extracts from Pitanga leaves were obtained by hydrodistillation and by extraction with supercritical carbon dioxide (SC-CO2) at three conditions of temperature and pressure. In the SC-CO2 extractions also were collected the components that are lost with the CO2 in the exit of the system using Porapak-Q polymer trap. All extracts were analyzed by gas chromatography-mass spectrometry (GC-MS). Thirty-nine compounds were found in the extracts and twenty-six were identified. The main components identified in the extracts in decreasing quantitative order were: curzerene, germacrene B, C15H20O2 andβ-elemene for hydrodistillation; C15H20O2 and curzerene for SC-CO2 extracts and 3-hexen-1-ol, curzerene, C15H20O2,β-elemene and germacrene B for SC-CO2 extracts captured in Porapak-Q. PRACTICAL APPLICATIONS The natural extracts are a potential source of compounds possessing biological activities. They can be used in foods, pharmaceutics and cosmetics. Pitanga is an Exotic Fruit from Brazil and extracts from its leaves have been used against many diseases in Brazilian folk medicine. Supercritical extraction is an interesting process for the production of natural extracts because it is a clean process and the knowledge of composition of extracts is crucial for the identification of the probable active components.
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supercritical co2 extraction of carotenoids from pitanga Fruits eugenia uniflora l
Journal of Supercritical Fluids, 2008Co-Authors: Genival Lopes Filho, Veridiana Vera De Rosso, Alessandra Lopes De Oliveira, Adriana Zerlotti Mercadante, Paulo T V Rosa, Angela M A Meireles, Fernando A CabralAbstract:Supercritical carbon dioxide (SC-CO2) extraction was employed to extract carotenoids from the freezedried pulp of pitanga Fruits (Eugenia uniflora L.), an Exotic Fruit, rich in carotenoids and still little explored commercially. The SC-CO2 extraction was carried out at two temperatures, 40 and 60 ◦C, and seven pressures, 100, 150, 200, 250, 300, 350 and 400 bar. The carotenoids were determined by high-performance liquid chromatography connected to photodiode array and mass spectrometry detectors. Lycopene, rubixanthin and -cryptoxanthin were the main carotenoids present in the freeze-dried pitanga pulp, whereas -cryptoxanthin concentration was negligible in the SC-CO2 extracts, for all the investigated state conditions. The maximum recovery of carotenoids was obtained at 60 ◦ C and 250 bar, extracting 55% of the total carotenoid content, 74% of the rubixanthin and 78% of the lycopene from the pulp. Under these state conditions, the total carotenoid concentration in the extract was 5474g/g, represented by 66% lycopene and 32% rubixanthin. The experimental state conditions produced different SC-CO2 extracts with respect to the extraction yield and concentration of different carotenoids, indicating that the supercritical carbon dioxide was selective in the extraction of the pitanga carotenoids as a function of temperature and pressure.
Karent Bravo - One of the best experts on this subject based on the ideXlab platform.
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characterization of polyphenol oxidase from cape gooseberry physalis peruviana l Fruit
Food Chemistry, 2016Co-Authors: Karent Bravo, Edison OsorioAbstract:Abstract Cape gooseberry ( Physalis peruviana ) is an Exotic Fruit highly valued, however it is a very rich source of polyphenol oxidase (PPO). In this study, Cape gooseberry PPO was isolated and biochemically characterized. The enzyme was extracted and purified using acetone and aqueous two-phase systems. The data indicated that PPO had the highest substrate affinity for chlorogenic acid, 4-methylcatechol and catechol. Chlorogenic acid was the most suitable substrate ( K m = 0.56 ± 0.07 mM and V max = 53.15 ± 2.03 U PPO mL −1 min −1 ). The optimal pH values were 5.5 for catechol and 4-methylcatechol and 5.0 for chlorogenic acid. Optimal temperatures were 40 °C for catechol, 25 °C for 4-methylcatechol and 20 °C for chlorogenic acid. In inhibition tests, the most potent inhibitor was found to be ascorbic acid followed by l -cysteine and quercetin. This study shows possible treatments that can be implemented during the processing of Cape gooseberry Fruits to prevent browning.
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influence of cultivar and ripening time on bioactive compounds and antioxidant properties in cape gooseberry physalis peruviana l
Journal of the Science of Food and Agriculture, 2015Co-Authors: Karent Bravo, Stella Sepulvedaortega, Oscar J Laraguzman, Alejandro A Navasarboleda, Edison OsorioAbstract:BACKGROUND Cape gooseberry (Physalis peruviana) is an Exotic Fruit highly valued for its organoleptic properties and bioactive compounds. Considering that the presence of phenolics and ascorbic acid could contribute to its functional capacity, it is important to investigate the quality parameters, bioactive contents and functional properties with respect to genotype and ripening time. In this study the genotype effect was evaluated in 15 cultivars for two different harvest times. Changes during maturation were recorded in two commercial cultivars within seven levels of maturity. RESULTS Multivariate statistical analysis suggested that phenolic content and ORAC value were mainly affected by harvest time and that ascorbic acid content and DPPH level were mainly affected by genotype. In addition, acidity, phenolic content, ORAC value and inhibition of LDL oxidation decreased with maturity, but soluble solids content, ascorbic acid content, β-carotene content and DPPH-scavenging activity were higher in mature Fruits. CONCLUSION The phenolic content, ascorbic acid content and antioxidant properties of Cape gooseberry Fruit were strongly affected by cultivar, harvest time and maturity state. Consequently, the harvest time must be scheduled carefully to gain the highest proportion of bioactive compounds according to the specific cultivar and the environment where it is grown. © 2014 Society of Chemical Industry
Christian Borgemeister - One of the best experts on this subject based on the ideXlab platform.
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taxonomy ecology and management of native and Exotic Fruit fly species in africa
Annual Review of Entomology, 2016Co-Authors: Sunday Ekesi, Marc De Meyer, Samira A Mohamed, M Virgilio, Christian BorgemeisterAbstract:Horticulture is one of the most important agricultural subsectors in Africa, providing income, creating employment opportunities, and enhancing food and nutritional security. However, tephritid Fruit flies are responsible for both direct and indirect losses, with alien invasive species often having the most severe ecological and economic impact. In the past 20 years, systematic analysis of tephritids has provided comparative information on taxonomy, synonymy, and character-state differentiation. New molecular techniques are now available for identifying species, reconstructing phylogenies, and studying population genetic structures. Research on biology, host range and shifts, thermotolerance, and demography has provided useful information for developing predictive and ecological niche models to guide management methods. In recent years, the responses of various species to attractants have been documented. Several suppression methods, including the release of coevolved parasitoid species targeting invasives, have been promoted within the context of integrated pest management, leading to improvement in the quality and quantity of Fruits and vegetables produced. However, there is still the need for wide-scale availability of these technologies to smallholder growers across Africa.