The Experts below are selected from a list of 21 Experts worldwide ranked by ideXlab platform
I A Morozov - One of the best experts on this subject based on the ideXlab platform.
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permeability of the gastrointestinal tract to macromolecules in Experimental Anaphylaxis
Patologicheskaia fiziologiia i èksperimental'naia terapiia, 1995Co-Authors: I V Gmoshinskiĭ, Iu A Lysikov, V K Mazo, I A MorozovAbstract:Rats were sensitized with single or multiple intraperitoneal administration of chick's egg ovalbumin (OA) or DNP-conjugated bovine serum albumin (DNP-BSA) on aluminium. The animals were challenged 14-28 days thereafter by intragastric or intravenous administration of respective antigens. Gastrointestinal barrier permeability was tested with macromolecular probes: OA and polyethylene glycol (PEG)-4000. The increase of permeability was revealed in animals sensitized with DNP-BSA and challenged intragastrically. The morphological view of intestinal damage included epithelial edema, exfoliation on the upper third part of the villi and also increases in the count of transepithelial lymphocytes. In OA-sensitized rats, the gastrointestinal permeability to PEG-4000 grew only following multiple sensitization and intravenous (or multiple intragastric) challenge with this antigen.
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effects of dietary fat on permeability of the protective intestinal barrier to macromolecules in Experimental Anaphylaxis
Voprosy pitaniia, 1995Co-Authors: R A Ermekpaeva, V K Mazo, Lysikov Iua, S N Kulakova, I A MorozovAbstract:Wistar rats were sensitized with hen egg ovalbumin (OVA) intraperitoneally and were fed with diets that contained 12% fish oil (FO) or sunflower oil for 3 weeks with a purpose to study the influence of dietary fat source on the macromolecular permeability of small intestine in systemic Anaphylaxis. 29 days later the systemic Anaphylaxis was challenged by i.v. administration of OVA. This reaction was aggravated in animals fed with FO this being supported by morphofunctional changes pattern in small intestine. The deleterious effect on intestinal protective barrier efficiency against polyethyleneglycol 4000 macromolecules was noticed in rats fed with FO containing large amounts of n-3 PUFA. It's suggested that the prevention of deleterious effect of FO is possible by increase of dietary antioxidative factors consumption that might be taken into consideration during some kinds of dietary treatment.
V K Mazo - One of the best experts on this subject based on the ideXlab platform.
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the selenium haemostasis during Experimental Anaphylaxis reaction in rats treated with reduced glutathione and selenium enriched spirulina
Voprosy medit︠s︡inskoĭ khimii, 2000Co-Authors: N A Golubkina, I V Gmoshinskiĭ, V K Mazo, S N Zorin, Kh A Tambiev, N N KirikovaAbstract:The main events caused by anaphilaxis in selenium haemostasis in rats include significant increase of selenium excretion with urine (6.36 +/- 1.18 nM Se/18 h., n = 10, compared with 1.72 +/- 0.38 nM Se/18 h., n = 10) and decrease of selenium plasma/selenium erythrocytes ratio from 0.939 to 0.791. Reduced glutathione (G-SH) administration led to 1.5-fold decrease of plasma selenium level and 1.3-fold increase of selenium concentration in intestinal walls of sensitized rats (r = -0.720, P < 0.001). Chromatographic separation of plasma proteins showed that intragastric intubation of G-SH to sensibilized rats significantly decreased the protein P content and did not influence the concentration of Se-GSHPx, thus indicating the local selenium acceptor role of G-SH. G-SH administration did not influence the intestinal permeability in sensitised rats while use of complex additive: G-SH and selenium enriched spirulina--normalized the latter parameter and the ratio of protein P/Se-GSHPx in plasma.
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permeability of the gastrointestinal tract to macromolecules in Experimental Anaphylaxis
Patologicheskaia fiziologiia i èksperimental'naia terapiia, 1995Co-Authors: I V Gmoshinskiĭ, Iu A Lysikov, V K Mazo, I A MorozovAbstract:Rats were sensitized with single or multiple intraperitoneal administration of chick's egg ovalbumin (OA) or DNP-conjugated bovine serum albumin (DNP-BSA) on aluminium. The animals were challenged 14-28 days thereafter by intragastric or intravenous administration of respective antigens. Gastrointestinal barrier permeability was tested with macromolecular probes: OA and polyethylene glycol (PEG)-4000. The increase of permeability was revealed in animals sensitized with DNP-BSA and challenged intragastrically. The morphological view of intestinal damage included epithelial edema, exfoliation on the upper third part of the villi and also increases in the count of transepithelial lymphocytes. In OA-sensitized rats, the gastrointestinal permeability to PEG-4000 grew only following multiple sensitization and intravenous (or multiple intragastric) challenge with this antigen.
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effects of dietary fat on permeability of the protective intestinal barrier to macromolecules in Experimental Anaphylaxis
Voprosy pitaniia, 1995Co-Authors: R A Ermekpaeva, V K Mazo, Lysikov Iua, S N Kulakova, I A MorozovAbstract:Wistar rats were sensitized with hen egg ovalbumin (OVA) intraperitoneally and were fed with diets that contained 12% fish oil (FO) or sunflower oil for 3 weeks with a purpose to study the influence of dietary fat source on the macromolecular permeability of small intestine in systemic Anaphylaxis. 29 days later the systemic Anaphylaxis was challenged by i.v. administration of OVA. This reaction was aggravated in animals fed with FO this being supported by morphofunctional changes pattern in small intestine. The deleterious effect on intestinal protective barrier efficiency against polyethyleneglycol 4000 macromolecules was noticed in rats fed with FO containing large amounts of n-3 PUFA. It's suggested that the prevention of deleterious effect of FO is possible by increase of dietary antioxidative factors consumption that might be taken into consideration during some kinds of dietary treatment.
Hisashi Mihara - One of the best experts on this subject based on the ideXlab platform.
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a rapid and strong increase of plasminogen activator induced by Experimental Anaphylaxis in rabbits
Enzyme, 1992Co-Authors: Kazuhiro Shimaya, Hiroyuki Sumi, Masugi Maruyama, Hisashi MiharaAbstract:Anaphylactic shock was induced in rabbits by injecting bovine serum albumin (BSA) as an antigen. Measurements of the enzyme activities in the fibrinolytic system confirmed that a rapid and strong increase of plasminogen activator (PA) was induced during Anaphylaxis. The euglobulin fibrinolytic activity (EFA) as estimated by the plasminogen-rich fibrin plate method rose significantly, peaking at 15 min after the BSA injection (when the arterial pressure was minimum). However, EFA was not detected by the plasminogen-poor fibrin plate method. The tissue-type PA (t-PA) activity using the natural substrate plasminogen increased significantly with a peak at 15 min. The amidolytic activity also simultaneously increased significantly using the t-PA substrate, H-D-Ile-Pro-Arg-pNA. The plasminogen activator inhibitor (PAI) activity remained at baseline levels until 30 min, but rose fourfold at 90 min. The main plasma fibrinolytic enzyme which increased in Anaphylaxis was proved by zymography to be t-PA with a molecular weight (MW) of 69,000.
R A Ermekpaeva - One of the best experts on this subject based on the ideXlab platform.
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effects of dietary fat on permeability of the protective intestinal barrier to macromolecules in Experimental Anaphylaxis
Voprosy pitaniia, 1995Co-Authors: R A Ermekpaeva, V K Mazo, Lysikov Iua, S N Kulakova, I A MorozovAbstract:Wistar rats were sensitized with hen egg ovalbumin (OVA) intraperitoneally and were fed with diets that contained 12% fish oil (FO) or sunflower oil for 3 weeks with a purpose to study the influence of dietary fat source on the macromolecular permeability of small intestine in systemic Anaphylaxis. 29 days later the systemic Anaphylaxis was challenged by i.v. administration of OVA. This reaction was aggravated in animals fed with FO this being supported by morphofunctional changes pattern in small intestine. The deleterious effect on intestinal protective barrier efficiency against polyethyleneglycol 4000 macromolecules was noticed in rats fed with FO containing large amounts of n-3 PUFA. It's suggested that the prevention of deleterious effect of FO is possible by increase of dietary antioxidative factors consumption that might be taken into consideration during some kinds of dietary treatment.
I V Gmoshinskiĭ - One of the best experts on this subject based on the ideXlab platform.
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the selenium haemostasis during Experimental Anaphylaxis reaction in rats treated with reduced glutathione and selenium enriched spirulina
Voprosy medit︠s︡inskoĭ khimii, 2000Co-Authors: N A Golubkina, I V Gmoshinskiĭ, V K Mazo, S N Zorin, Kh A Tambiev, N N KirikovaAbstract:The main events caused by anaphilaxis in selenium haemostasis in rats include significant increase of selenium excretion with urine (6.36 +/- 1.18 nM Se/18 h., n = 10, compared with 1.72 +/- 0.38 nM Se/18 h., n = 10) and decrease of selenium plasma/selenium erythrocytes ratio from 0.939 to 0.791. Reduced glutathione (G-SH) administration led to 1.5-fold decrease of plasma selenium level and 1.3-fold increase of selenium concentration in intestinal walls of sensitized rats (r = -0.720, P < 0.001). Chromatographic separation of plasma proteins showed that intragastric intubation of G-SH to sensibilized rats significantly decreased the protein P content and did not influence the concentration of Se-GSHPx, thus indicating the local selenium acceptor role of G-SH. G-SH administration did not influence the intestinal permeability in sensitised rats while use of complex additive: G-SH and selenium enriched spirulina--normalized the latter parameter and the ratio of protein P/Se-GSHPx in plasma.
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permeability of the gastrointestinal tract to macromolecules in Experimental Anaphylaxis
Patologicheskaia fiziologiia i èksperimental'naia terapiia, 1995Co-Authors: I V Gmoshinskiĭ, Iu A Lysikov, V K Mazo, I A MorozovAbstract:Rats were sensitized with single or multiple intraperitoneal administration of chick's egg ovalbumin (OA) or DNP-conjugated bovine serum albumin (DNP-BSA) on aluminium. The animals were challenged 14-28 days thereafter by intragastric or intravenous administration of respective antigens. Gastrointestinal barrier permeability was tested with macromolecular probes: OA and polyethylene glycol (PEG)-4000. The increase of permeability was revealed in animals sensitized with DNP-BSA and challenged intragastrically. The morphological view of intestinal damage included epithelial edema, exfoliation on the upper third part of the villi and also increases in the count of transepithelial lymphocytes. In OA-sensitized rats, the gastrointestinal permeability to PEG-4000 grew only following multiple sensitization and intravenous (or multiple intragastric) challenge with this antigen.
