The Experts below are selected from a list of 31080 Experts worldwide ranked by ideXlab platform
Caroline C. Whitacre - One of the best experts on this subject based on the ideXlab platform.
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The thymus plays a role in oral tolerance induction in Experimental Autoimmune Encephalomyelitis.
Annals of the New York Academy of Sciences, 2004Co-Authors: Fei Song, Ingrid E. Gienapp, Todd Shawler, Zhen Guan, Caroline C. WhitacreAbstract:Mice are protected from Experimental Autoimmune Encephalomyelitis (EAE) when fed myelin basic protein (MBP). Thymectomized mice do not exhibit oral tolerance. We found evidence for two mechanisms to explain the role of the thymus in oral tolerance: a site for deletion of autoreactive T cells and a source of regulatory T cells.
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Oral tolerance as therapy for Experimental Autoimmune Encephalomyelitis and multiple sclerosis: Demonstration of T cell anergy
Immunology and cell biology, 1998Co-Authors: Scott Jewell, Ingrid E. Gienapp, Karen Cox, Caroline C. WhitacreAbstract:Oral tolerance as therapy for Experimental Autoimmune Encephalomyelitis and multiple sclerosis: Demonstration of T cell anergy
Ingrid E. Gienapp - One of the best experts on this subject based on the ideXlab platform.
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The thymus plays a role in oral tolerance induction in Experimental Autoimmune Encephalomyelitis.
Annals of the New York Academy of Sciences, 2004Co-Authors: Fei Song, Ingrid E. Gienapp, Todd Shawler, Zhen Guan, Caroline C. WhitacreAbstract:Mice are protected from Experimental Autoimmune Encephalomyelitis (EAE) when fed myelin basic protein (MBP). Thymectomized mice do not exhibit oral tolerance. We found evidence for two mechanisms to explain the role of the thymus in oral tolerance: a site for deletion of autoreactive T cells and a source of regulatory T cells.
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Oral tolerance as therapy for Experimental Autoimmune Encephalomyelitis and multiple sclerosis: Demonstration of T cell anergy
Immunology and cell biology, 1998Co-Authors: Scott Jewell, Ingrid E. Gienapp, Karen Cox, Caroline C. WhitacreAbstract:Oral tolerance as therapy for Experimental Autoimmune Encephalomyelitis and multiple sclerosis: Demonstration of T cell anergy
William J. Karpus - One of the best experts on this subject based on the ideXlab platform.
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Cytokines and Chemokines in the Pathogenesis of Experimental Autoimmune Encephalomyelitis
Journal of immunology (Baltimore Md. : 1950), 2020Co-Authors: William J. KarpusAbstract:Experimental Autoimmune Encephalomyelitis is a CD4+ T cell-mediated demyelinating disease of the CNS that serves as a model for multiple sclerosis. Cytokines and chemokines shape Th1 and Th17 effector responses as well as regulate migration of leukocytes to the CNS during disease. The CNS cellular infiltrate consists of Ag-specific and nonspecific CD4+ and CD8+ T cells, neutrophils, B cells, monocytes, macrophages, and dendritic cells. The mechanism of immune-mediated inflammation in Experimental Autoimmune Encephalomyelitis has been extensively studied in an effort to develop therapeutic modalities for multiple sclerosis and, indeed, has provided insight in modern drug discovery. The present Brief Review highlights critical pathogenic aspects of cytokines and chemokines involved in generation of effector T cell responses and migration of inflammatory cells to the CNS. Select cytokines and chemokines are certainly important in the regulatory response, which involves T regulatory, B regulatory, and myeloid-derived suppressor cells. However, that discussion is beyond the scope of this brief review.
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Inflammatory macrophage migration in Experimental Autoimmune Encephalomyelitis.
Methods in molecular biology (Clifton N.J.), 2013Co-Authors: William J. KarpusAbstract:Abstract Experimental Autoimmune Encephalomyelitis (EAE) is a CD4 T cell-mediated demyelinating disease of the central nervous system (CNS) where macrophages are the end-stage effector cell. EAE serves as a model for multiple sclerosis where it has been instructive in delineating the Autoimmune cellular response in the CNS for the purpose of developing more effective therapies. Understanding the nature of how cytokine and chemokine networks regulate the migration of leukocytes to the CNS requires the ability to track subpopulations of those cells in vivo. We describe a flow cytometric technique to monitor the migration of macrophages during EAE development.
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Experimental Autoimmune Encephalomyelitis in the mouse.
Current protocols in immunology, 2010Co-Authors: Stephen D. Miller, William J. Karpus, Todd Scott DavidsonAbstract:This unit details the materials and methods required for both active induction and adoptive transfer of Experimental Autoimmune Encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Detailed materials and methods required for the purification of both PLP and MBP are also described. A protocol for isolating CNS-infiltrating lymphocytes in EAE mice is included. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains.
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UNIT 15.1 Experimental Autoimmune Encephalomyelitis in the Mouse
Current Protocols in Immunology, 2007Co-Authors: Stephen D. Miller, William J. KarpusAbstract:This unit details the materials and methods required for both active induction and adoptive transfer of Experimental Autoimmune Encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Detailed materials and methods required for the purification of both PLP and MBP are also described. A protocol for isolating CNS-infiltrating lymphocytes in EAE mice is included. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains. Curr. Protoc. Immunol. 88:15.1.1-15.1.20. © 2010 by John Wiley & Sons, Inc. Keywords: CFA; complete Freund's adjuvant; CNS; central nervous system; EAE; Experimental Autoimmune Encephalomyelitis; MBP; myelin basic protein; MOG; myelin oligodendrocyte glycoprotein; PLP; proteolipid protein
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Regulation of Experimental Autoimmune Encephalomyelitis by chemokines and chemokine receptors.
Immunologic research, 2002Co-Authors: Adam Elhofy, Kevin J. Kennedy, Brian T. Fife, William J. KarpusAbstract:Experimental Autoimmune Encephalomyelitis (EAE) is a T cell mediated demyelinating disease of the central nervous system (CNS) that serves as a model for multiple sclerosis (MS). Insights into the pathogenesis of this model may help scientists understand the human disease and aid in rational drug discovery. In this review we summarize the role of chemokines and chemokine receptors in disease pathogenesis and suggest a pathway of events that leads to demyelination and subsequent clinical disease manifestation.
Fei Song - One of the best experts on this subject based on the ideXlab platform.
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The thymus plays a role in oral tolerance induction in Experimental Autoimmune Encephalomyelitis.
Annals of the New York Academy of Sciences, 2004Co-Authors: Fei Song, Ingrid E. Gienapp, Todd Shawler, Zhen Guan, Caroline C. WhitacreAbstract:Mice are protected from Experimental Autoimmune Encephalomyelitis (EAE) when fed myelin basic protein (MBP). Thymectomized mice do not exhibit oral tolerance. We found evidence for two mechanisms to explain the role of the thymus in oral tolerance: a site for deletion of autoreactive T cells and a source of regulatory T cells.
Jolie A. Stepaniak - One of the best experts on this subject based on the ideXlab platform.
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Experimental Autoimmune Encephalomyelitis in the rat.
Current protocols in immunology, 2009Co-Authors: Mark Mannie, Robert H. Swanborg, Jolie A. StepaniakAbstract:There are several diverse rat models of Experimental Autoimmune Encephalomyelitis (EAE) that can be used to investigate the pathogenesis and regulation of autoimmunity against CNS myelin. The disease course of these models ranges from an acute monophasic disease with limited demyelination to a chronic relapsing or chronic progressive course marked by severe demyelination. These models enable the study of encephalitogenic T cells and demyelinating antibody specific for major neuroantigens such as myelin basic protein (MBP), myelin oligodendrocyte glycoprotein (MOG), or proteolipid protein (PLP), among other important CNS autoantigens. Overall, this unit provides an overview of common methods for induction of active and passive EAE, assessment and analysis of clinical disease, preparation and purification of myelin basic protein, and derivation of neuroantigen-specific rat T cell lines. This unit also provides a brief discussion of the basic characteristics of these models.
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Current Protocols in Immunology - Experimental Autoimmune Encephalomyelitis in the Rat
Current Protocols in Immunology, 1996Co-Authors: Robert H. Swanborg, Jolie A. StepaniakAbstract:This unit details the materials and methods required for both active induction and adoptive transfer of Experimental Autoimmune Encephalomyelitis (EAE) in the SJL mouse strain using intact proteins or peptides from the two major myelin proteins: proteolipid protein (PLP) and myelin basic protein (MBP). Detailed materials and methods required for the purification of both PLP and MBP are also described. Modifications of the specified protocols may be necessary for efficient induction of active or adoptive EAE in other mouse strains.
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Variable susceptibility of Lewis rats to Experimental Autoimmune Encephalomyelitis
Journal of neuroimmunology, 1994Co-Authors: Karen E. Gould, Jolie A. Stepaniak, Robert H. SwanborgAbstract:Abstract This report is to alert other investigators that Lewis rats, which are widely employed in studies of Autoimmune diseases, very considerably with respect to susceptibility to Experimental Autoimmune Encephalomyelitis (EAE), depending upon commercial source.
